Institut für Neuropathologie

[["dc.bibliographiccitation.artnumber","andr.13292"],["dc.bibliographiccitation.firstpage","1660"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Andrology"],["dc.bibliographiccitation.lastpage","1672"],["dc.bibliographiccitation.volume","10"],["dc.contributor.affiliation","Pinkert‐Leetsch, Diana; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Rost, John Uwe; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Schmiedeknecht, Max Ulrich Heiner; 3\r\nDepartment of Neuropathology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Stadelmann, Christine; 3\r\nDepartment of Neuropathology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.affiliation","Alves, Frauke; 1\r\nDepartment of Diagnostic and Interventional Radiology\r\nUniversity Medical Center Goettingen\r\nGoettingen Germany"],["dc.contributor.author","Pinkert‐Leetsch, Diana"],["dc.contributor.author","Rost, John Uwe"],["dc.contributor.author","Schmiedeknecht, Max Ulrich Heiner"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Alves, Frauke"],["dc.contributor.author","Missbach‐Guentner, Jeannine"],["dc.date.accessioned","2022-11-28T08:48:04Z"],["dc.date.available","2022-11-28T08:48:04Z"],["dc.date.issued","2022-09-15"],["dc.date.updated","2022-11-27T10:10:46Z"],["dc.description.abstract","Abstract\r\n\r\nBackground\r\nThe unique anatomy of the male reproductive organ reflects its complex function from sperm maturation to their storage for months until emission. Since light microscopy in two dimensions (2d) cannot sufficiently demonstrate its complex morphology, a comprehensive visualization is required to identify pathologic alterations in its entire anatomical context.\r\n\r\n\r\nObjectives\r\nAim of this study was to use three‐dimensional (3d) light sheet fluorescence microscopy (LSFM) to visualize entire murine testes in 3d, label‐free and at subcellular resolution, and to assign local autofluorescence to testicular and deferent structures.\r\n\r\n\r\nMaterials and methods\r\nMurine testes were fixed with four different fixatives and subsequently cleared with benzoic acid/benzyl benzoate. Hereafter, complete murine testes were scanned with LSFM with different fluorescence filter sets and subsequently embedded in paraffin for further conventional planar histology.\r\n\r\n\r\nResults\r\nAutofluorescence signals of the murine reproductive organ allowed the unambiguous identification of the testicular anatomy from the seminiferous tubules to the vas deferens with their specific stratification independent of the used fixative. Blood vessels were visualized from the pampiniform plexus to the small capillaries of single tubules. Moreover, due to the specific intrinsic fluorescence properties of the efferent ducts and the epididymis, luminal caliber, the epithelial stratification and retronuclear cytoplasmic inclusions gave a unique insight into the interface of both morphological structures. Subsequent 2d histology confirmed the identified morphological structures.\r\n\r\n\r\nDiscussion\r\nLSFM analysis of the murine reproductive organ allows due to its intrinsic fluorescence a simple, label‐free 3d assessment of its entire duct morphology, the epithelial composition, and the associated blood supply in its anatomical relation.\r\n\r\n\r\nConclusion\r\nLSFM provides the technical basis for comprehensive analyses of pathologically altered murine testes in its entirety by depicting specific autofluorescence. Thereby it facilitates mouse studies of testicular disease or their drug‐related alterations in more detail potentially for clinical translation assessing human testicular biopsies."],["dc.description.sponsorship","Bundesministerium fuer Bildung und Forschung, Deutschland"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/andr.13292"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/117280"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-600"],["dc.relation.eissn","2047-2927"],["dc.relation.issn","2047-2919"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made."],["dc.title","The murine male reproductive organ at a glance: Three‐dimensional insights and virtual histology using label‐free light sheet microcopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","923"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Acta Neuropathologica"],["dc.bibliographiccitation.lastpage","936"],["dc.bibliographiccitation.volume","142"],["dc.contributor.author","Schwabenland, Marius"],["dc.contributor.author","Brück, Wolfgang"],["dc.contributor.author","Priller, Josef"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Lassmann, Hans"],["dc.contributor.author","Prinz, Marco"],["dc.date.accessioned","2021-12-01T09:23:07Z"],["dc.date.available","2021-12-01T09:23:07Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract As extremely sensitive immune cells, microglia act as versatile watchdogs of the central nervous system (CNS) that tightly control tissue homeostasis. Therefore, microglial activation is an early and easily detectable hallmark of virtually all neuropsychiatric, neuro-oncological, neurodevelopmental, neurodegenerative and neuroinflammatory diseases. The recent introduction of novel high-throughput technologies and several single-cell methodologies as well as advances in epigenetic analyses helped to identify new microglia expression profiles, enhancer-landscapes and local signaling cues that defined diverse previously unappreciated microglia states in the healthy and diseased CNS. Here, we give an overview on the recent developments in the field of microglia biology and provide a practical guide to analyze disease-associated microglia phenotypes in both the murine and human CNS, on several morphological and molecular levels. Finally, technical limitations, potential pitfalls and data misinterpretations are discussed as well."],["dc.description.abstract","Abstract As extremely sensitive immune cells, microglia act as versatile watchdogs of the central nervous system (CNS) that tightly control tissue homeostasis. Therefore, microglial activation is an early and easily detectable hallmark of virtually all neuropsychiatric, neuro-oncological, neurodevelopmental, neurodegenerative and neuroinflammatory diseases. The recent introduction of novel high-throughput technologies and several single-cell methodologies as well as advances in epigenetic analyses helped to identify new microglia expression profiles, enhancer-landscapes and local signaling cues that defined diverse previously unappreciated microglia states in the healthy and diseased CNS. Here, we give an overview on the recent developments in the field of microglia biology and provide a practical guide to analyze disease-associated microglia phenotypes in both the murine and human CNS, on several morphological and molecular levels. Finally, technical limitations, potential pitfalls and data misinterpretations are discussed as well."],["dc.identifier.doi","10.1007/s00401-021-02370-8"],["dc.identifier.pii","2370"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94564"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","1432-0533"],["dc.relation.issn","0001-6322"],["dc.title","Analyzing microglial phenotypes across neuropathologies: a practical guide"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Journal of Neurology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Schirmer, Lucas"],["dc.contributor.author","Koenig, Fatima Barbara"],["dc.contributor.author","Merkler, Doron"],["dc.contributor.author","Brueck, Wolfgang"],["dc.contributor.author","Stadelmann, Christine"],["dc.date.accessioned","2018-11-07T08:39:40Z"],["dc.date.available","2018-11-07T08:39:40Z"],["dc.date.issued","2010"],["dc.format.extent","657"],["dc.identifier.isi","000293331101589"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19052"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.eventlocation","Geneva, SWITZERLAND"],["dc.relation.issn","1351-5101"],["dc.title","Comparative study of neuronal and axonal pathology in early multiple sclerosis and CNS trauma lesions"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","296"],["dc.bibliographiccitation.issue","7794"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","300"],["dc.bibliographiccitation.volume","578"],["dc.contributor.author","Yasuda, Sayaka"],["dc.contributor.author","Tsuchiya, Hikaru"],["dc.contributor.author","Kaiho, Ai"],["dc.contributor.author","Guo, Qiang"],["dc.contributor.author","Ikeuchi, Ken"],["dc.contributor.author","Endo, Akinori"],["dc.contributor.author","Arai, Naoko"],["dc.contributor.author","Ohtake, Fumiaki"],["dc.contributor.author","Murata, Shigeo"],["dc.contributor.author","Inada, Toshifumi"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Tanaka, Keiji"],["dc.contributor.author","Saeki, Yasushi"],["dc.date.accessioned","2020-12-10T18:10:02Z"],["dc.date.available","2020-12-10T18:10:02Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41586-020-1982-9"],["dc.identifier.pmid","32025036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73832"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/30"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","Stress- and ubiquitylation-dependent phase separation of the proteasome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1877"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Journal of Neuroscience Research"],["dc.bibliographiccitation.lastpage","1883"],["dc.bibliographiccitation.volume","87"],["dc.contributor.author","Tauber, Simone C."],["dc.contributor.author","Bunkowski, Stephanie"],["dc.contributor.author","Ebert, Sandra"],["dc.contributor.author","Schulz, Daniela"],["dc.contributor.author","Kellert, Benedikt"],["dc.contributor.author","Nau, Roland"],["dc.contributor.author","Gerber, Joachim"],["dc.date.accessioned","2018-11-07T08:29:27Z"],["dc.date.available","2018-11-07T08:29:27Z"],["dc.date.issued","2009"],["dc.description.abstract","An increase in adult neurogenesis was observed after exposure to enriched environment (EE) and during reconvalescence from experimental pneumococcal meningitis. This study investigated neurogenesis and spatial learning performance 5 weeks after bacterial meningitis and exposure to EE. C57BL/6 mice were infected by intracerebral injection of Streptococcus pneumoniae and treated with ceftriaxone for 5 days. Forty-eight hours after infection, one group (n = 22) was exposed to EE and the other group (n = 23) housed under standard conditions. Another set of mice was kept under either enriched (n = 16) or standard (n = 15) conditions without bacterial meningitis. Five weeks later, the Morris water maze was performed, and neurogenesis was evaluated by means of immunohistochemistry. Mice housed in EE without prior bacterial infection displayed both increased neurogenesis and improved water maze performance in comparison with uninfected control animals. Bacterial meningitis stimulated neurogenesis in the granular cell layer of the dentate gyrus: with standard housing conditions, we observed a higher density of BrdU-immunolabeled and TUC-4-expressing cells 5 weeks after induction of bacterial meningitis than in the noninfected control group. EE did not further increase progenitor cell proliferation and neuronal differentiation in the subgranular cell layer of the dentate gyrus after bacterial meningitis in comparison with infected mice housed under standard conditions. Moreover, the Morris water maze showed no significant differences between survivors of meningitis exposed to EE and animals kept in standard housing. In summary, exposure to EE after pneumococcal meningitis did not further increase meningitis-induced neurogenesis or improve spatial learning. (C) 2009 Wiley-Liss, Inc."],["dc.description.sponsorship","Else Kroner-Fresenius-Stiftung [1161/06//A70/06]"],["dc.identifier.doi","10.1002/jnr.22010"],["dc.identifier.isi","000265462800015"],["dc.identifier.pmid","19170185"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16657"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0360-4012"],["dc.title","Enriched Environment Fails To Increase Meningitis-Induced Neurogenesis and Spatial Memory in a Mouse Model of Pneumococcal Meningitis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","e1007962"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS Computational Biology"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Salfer, Maria"],["dc.contributor.author","Collado, Javier F."],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Martínez-Sánchez, Antonio"],["dc.date.accessioned","2021-04-14T08:23:54Z"],["dc.date.available","2021-04-14T08:23:54Z"],["dc.date.issued","2020"],["dc.description.abstract","Curvature is a fundamental morphological descriptor of cellular membranes. Cryo-electron tomography (cryo-ET) is particularly well-suited to visualize and analyze membrane morphology in a close-to-native state and molecular resolution. However, current curvature estimation methods cannot be applied directly to membrane segmentations in cryo-ET, as these methods cannot cope with some of the artifacts introduced during image acquisition and membrane segmentation, such as quantization noise and open borders. Here, we developed and implemented a Python package for membrane curvature estimation from tomogram segmentations, which we named PyCurv. From a membrane segmentation, a signed surface (triangle mesh) is first extracted. The triangle mesh is then represented by a graph, which facilitates finding neighboring triangles and the calculation of geodesic distances necessary for local curvature estimation. PyCurv estimates curvature based on tensor voting. Beside curvatures, this algorithm also provides robust estimations of surface normals and principal directions. We tested PyCurv and three well-established methods on benchmark surfaces and biological data. This revealed the superior performance of PyCurv not only for cryo-ET, but also for data generated by other techniques such as light microscopy and magnetic resonance imaging. Altogether, PyCurv is a versatile open-source software to reliably estimate curvature of membranes and other surfaces in a wide variety of applications."],["dc.identifier.doi","10.1371/journal.pcbi.1007962"],["dc.identifier.pmid","32776920"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81095"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/59"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","1553-7358"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.rights","CC BY 4.0"],["dc.title","Reliable estimation of membrane curvature for cryo-electron tomography"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","399"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Current Neurology and Neuroscience Reports"],["dc.bibliographiccitation.lastpage","404"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Wegner, Christiane"],["dc.contributor.author","Stadelmann, Christine"],["dc.date.accessioned","2018-11-07T11:24:39Z"],["dc.date.available","2018-11-07T11:24:39Z"],["dc.date.issued","2009"],["dc.description.abstract","Gray matter demyelination is frequent and extensive in most patients with multiple sclerosis (MS) and has recently received much attention in neuropathologic and imaging studies. Gray matter lesions show distinct pathologic features that make their detection difficult with conventional imaging techniques. Thus, despite their high prevalence, their impact on clinical symptoms has not been defined well so far. This review focuses on recent information from pathologic and imaging studies and summarizes our current knowledge on cortical pathology derived from human and experimental studies."],["dc.identifier.doi","10.1007/s11910-009-0058-x"],["dc.identifier.isi","000268572700008"],["dc.identifier.pmid","19664370"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56452"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1528-4042"],["dc.title","Gray matter pathology and multiple sclerosis"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","865"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Infection and Immunity"],["dc.bibliographiccitation.lastpage","871"],["dc.bibliographiccitation.volume","78"],["dc.contributor.author","Ribes, Sandra"],["dc.contributor.author","Ebert, Sandra"],["dc.contributor.author","Regen, Tommy"],["dc.contributor.author","Agarwal, Amit"],["dc.contributor.author","Tauber, Simone C."],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Spreer, Annette"],["dc.contributor.author","Bunkowski, Stephanie"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Hanisch, Uwe-Karsten"],["dc.contributor.author","Hammerschmidt, Sven"],["dc.contributor.author","Nau, Roland"],["dc.date.accessioned","2018-11-07T08:46:16Z"],["dc.date.available","2018-11-07T08:46:16Z"],["dc.date.issued","2010"],["dc.description.abstract","Toll-like receptors (TLRs) are crucial pattern recognition receptors in innate immunity that are expressed in microglia, the resident macrophages of the brain. TLR2, -4, and -9 are important in the responses against Streptococcus pneumoniae, the most common agent causing bacterial meningitis beyond the neonatal period. Murine microglial cultures were stimulated with agonists for TLR1/2 (Pam3CSK4), TLR4 (lipopolysaccharide), and TLR9 (CpG oligodeoxynucleotide) for 24 h and then exposed to either the encapsulated D39 (serotype 2) or the nonencapsulated R6 strain of S. pneumoniae. After stimulation, the levels of interleukin-6 and CCL5 (RANTES [regulated upon activation normal T-cell expressed and secreted]) were increased, confirming microglial activation. The TLR1/2, -4, and -9 agonist-stimulated microglia ingested significantly more bacteria than unstimulated cells (P < 0.05). The presence of cytochalasin D, an inhibitor of actin polymerizaton, blocked >90% of phagocytosis. Along with an increased phagocytic activity, the intracellular bacterial killing was also increased in TLR-stimulated cells compared to unstimulated cells. Together, our data suggest that microglial stimulation by these TLRs may increase the resistance of the brain against pneumococcal infections."],["dc.identifier.doi","10.1128/IAI.01110-09"],["dc.identifier.isi","000273855600033"],["dc.identifier.pmid","19933834"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20648"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0019-9567"],["dc.title","Toll-Like Receptor Stimulation Enhances Phagocytosis and Intracellular Killing of Nonencapsulated and Encapsulated Streptococcus pneumoniae by Murine Microglia"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","768"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Annals of clinical and translational neurology"],["dc.bibliographiccitation.lastpage","783"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Malviya, Manish"],["dc.contributor.author","Barman, Sumanta"],["dc.contributor.author","Golombeck, Kristin S."],["dc.contributor.author","Planagumà, Jesús"],["dc.contributor.author","Mannara, Francesco"],["dc.contributor.author","Strutz-Seebohm, Nathalie"],["dc.contributor.author","Wrzos, Claudia"],["dc.contributor.author","Demir, Fatih"],["dc.contributor.author","Baksmeier, Christine"],["dc.contributor.author","Steckel, Julia"],["dc.contributor.author","Falk, Kim Kristin"],["dc.contributor.author","Gross, Catharina C."],["dc.contributor.author","Kovac, Stjepana"],["dc.contributor.author","Bönte, Kathrin"],["dc.contributor.author","Johnen, Andreas"],["dc.contributor.author","Wandinger, Klaus-Peter"],["dc.contributor.author","Martín-García, Elena"],["dc.contributor.author","Becker, Albert J."],["dc.contributor.author","Elger, Christian E."],["dc.contributor.author","Klöcker, Nikolaj"],["dc.contributor.author","Wiendl, Heinz"],["dc.contributor.author","Meuth, Sven G."],["dc.contributor.author","Hartung, Hans-Peter"],["dc.contributor.author","Seebohm, Guiscard"],["dc.contributor.author","Leypoldt, Frank"],["dc.contributor.author","Maldonado, Rafael"],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Dalmau, Josep"],["dc.contributor.author","Melzer, Nico"],["dc.contributor.author","Goebels, Norbert"],["dc.date.accessioned","2019-07-09T11:44:45Z"],["dc.date.available","2019-07-09T11:44:45Z"],["dc.date.issued","2017-11"],["dc.description.abstract","Objective: Autoimmune encephalitis is most frequently associated with anti-NMDAR autoantibodies. Their pathogenic relevance has been suggested by passive transfer of patients' cerebrospinal fluid (CSF) in mice in vivo. We aimed to analyze the intrathecal plasma cell repertoire, identify autoantibody-producing clones, and characterize their antibody signatures in recombinant form. Methods: Patients with recent onset typical anti-NMDAR encephalitis were subjected to flow cytometry analysis of the peripheral and intrathecal immune response before, during, and after immunotherapy. Recombinant human monoclonal antibodies (rhuMab) were cloned and expressed from matching immunoglobulin heavy- (IgH) and light-chain (IgL) amplicons of clonally expanded intrathecal plasma cells (cePc) and tested for their pathogenic relevance. Results: Intrathecal accumulation of B and plasma cells corresponded to the clinical course. The presence of cePc with hypermutated antigen receptors indicated an antigen-driven intrathecal immune response. Consistently, a single recombinant human GluN1-specific monoclonal antibody, rebuilt from intrathecal cePc, was sufficient to reproduce NMDAR epitope specificity in vitro. After intraventricular infusion in mice, it accumulated in the hippocampus, decreased synaptic NMDAR density, and caused severe reversible memory impairment, a key pathogenic feature of the human disease, in vivo. Interpretation: A CNS-specific humoral immune response is present in anti-NMDAR encephalitis specifically targeting the GluN1 subunit of the NMDAR. Using reverse genetics, we recovered the typical intrathecal antibody signature in recombinant form, and proved its pathogenic relevance by passive transfer of disease symptoms from man to mouse, providing the critical link between intrathecal immune response and the pathogenesis of anti-NMDAR encephalitis as a humorally mediated autoimmune disease."],["dc.identifier.doi","10.1002/acn3.444"],["dc.identifier.pmid","29159189"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14885"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59083"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2328-9503"],["dc.rights","CC BY-NC 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc/4.0"],["dc.subject.ddc","610"],["dc.title","NMDAR encephalitis: passive transfer from man to mouse by a recombinant antibody."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","793"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Annals of Neurology"],["dc.bibliographiccitation.lastpage","796"],["dc.bibliographiccitation.volume","49"],["dc.contributor.author","Bitsch, Annette"],["dc.contributor.author","Kuhlmann, T."],["dc.contributor.author","Stadelmann, Christine"],["dc.contributor.author","Lassmann, Hans"],["dc.contributor.author","Lucchinetti, C."],["dc.contributor.author","Bruck, Wolfgang W."],["dc.date.accessioned","2018-11-07T08:59:26Z"],["dc.date.available","2018-11-07T08:59:26Z"],["dc.date.issued","2001"],["dc.description.abstract","Severe tissue destruction is the presumed histopathological correlate of hypointense multiple sclerosis (MS) lesions. In this study we correlated changes of lesion hypointensity over time with initial histopathological features in 14 biopsied MS lesions. The extent of hypointensity increased in initially demyelinated plaques and decreased in remyelinating lesions. The initial axonal loss determined the increase of hypointensity over time. In conclusion, both axonal loss and demyelinating activity determine the evolution of hypointensity over time."],["dc.identifier.doi","10.1002/ana.1053"],["dc.identifier.isi","000169091400016"],["dc.identifier.pmid","11409432"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23896"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0364-5134"],["dc.title","A longitudinal MRI study of histopathologically defined hypointense multiple sclerosis lesions"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]