Karbstein, Kevin

[["dc.bibliographiccitation.firstpage","14435"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Ecology and Evolution"],["dc.bibliographiccitation.lastpage","14450"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Rahmsdorf, Elisabeth"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-04-14T08:30:57Z"],["dc.date.available","2021-04-14T08:30:57Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract The larger distribution area of asexuals compared with their sexual relatives in geographical parthenogenesis (GP) scenarios has been widely attributed to the advantages of uniparental reproduction and polyploidy. However, potential disadvantages of sexuals due to their breeding system have received little attention so far. Here, we study the breeding system of five narrowly distributed sexual lineages of Ranunculus notabilis s.l. (R. auricomus complex) and its effects on outcrossing, inbreeding, female fitness, and heterozygosity. We performed selfing and intra‐ and interlineage crossings by bagging 481 flowers (59 garden individuals) followed by germination experiments. We compared seed set and germination rates, and related them to genetic distance and genome‐wide heterozygosity (thousands of RADseq loci). Selfings (2.5%) unveiled a significantly lower seed set compared with intra‐ (69.0%) and interlineage crossings (69.5%). Seed set of intra‐ (65%) compared to interpopulation crossings (78%) was significantly lower. In contrast, all treatments showed comparable germination rates (32%–43%). Generalized linear regressions between seed set and genetic distance revealed positive relationships in general and between lineages, and a negative one within lineages. Seed set was the main decisive factor for female fitness. Germination rates were not related to genetic distance at any level, but were positively associated with heterozygosity in interlineage crossings. Experiments confirmed full crossability and predominant outcrossing among sexual R. notabilis s.l. lineages. However, up to 5% (outliers 15%–31%) of seeds were formed by selfing, probably due to semi‐self‐compatibility in a multi‐locus gametophytic SI system. Less seed set in intrapopulation crossings, and higher seed set and germination rates from crossings of genetically more distant and heterozygous lineages (interlineage) indicate negative inbreeding and positive outbreeding effects. In GP scenarios, sexual species with small and/or isolated populations can suffer from decreased female fitness due to their breeding system. This factor, among others, probably limits range expansion of sexuals."],["dc.description.abstract","Potential disadvantages of sexual plant species due to their breeding system have received little attention in geographical parthenogenesis (GP) scenarios so far. We compared seed set and germination rates of five sexual lineages of Ranunculus notabilis s.l (R. auricomus complex) and related them to genetic distance and genome‐wide heterozygosity. Results suggest that inbreeding depression particularly affects intrapopulation (intralineage) crossings, and that positive outbreeding effects particularly influence interlineage crossings. In GP scenarios, sexual species with small and/or isolated populations can suffer from decreased female fitness due to their breeding system. image"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1002/ece3.7073"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17784"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83429"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.notes.intern","Merged from goescholar"],["dc.notes.intern","In goescholar not merged with http://resolver.sub.uni-goettingen.de/purl?gs-1/17835 but duplicate"],["dc.relation.eissn","2045-7758"],["dc.relation.issn","2045-7758"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","570"],["dc.title","Breeding system of diploid sexuals within the Ranunculus auricomus complex and its role in a geographical parthenogenesis scenario"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2031"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Molecular Ecology"],["dc.bibliographiccitation.lastpage","2049"],["dc.bibliographiccitation.volume","29"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Paetzold, Claudia"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-04-14T08:25:24Z"],["dc.date.available","2021-04-14T08:25:24Z"],["dc.date.issued","2020"],["dc.description.abstract","Abstract The time frame and geographical patterns of diversification processes in European temperate‐montane herbs are still not well understood. We used the sexual species of the Ranunculus auricomus complex as a model system to understand how vicariance versus dispersal processes in the context of Pleistocene climatic fluctuations have triggered speciation in temperate‐montane plant species. We used target enrichment sequence data from about 600 nuclear genes and coalescent‐based species tree inference methods to resolve phylogenetic relationships among the sexual taxa of the complex. We estimated absolute divergence times and, using ancestral range reconstruction, we tested if speciation was enhanced by vicariance or by dispersal processes. Phylogenetic relationships among taxa were fully resolved with some incongruence in the position of the tetraploid R. marsicus. Speciation events took place in a very short time at the end of the Mid‐Pleistocene Transition (830–580 thousand years ago [ka]). A second wave of intraspecific geographical differentiation occurred at the end of the Riss glaciation or during the Eemian interglacial between 200 and 100 ka. Ancestral range reconstruction suggests a widespread European ancestor of the R. auricomus complex. Vicariance has triggered allopatric speciation in temperate‐montane plant species during the climatic deterioration that occurred during the last phase of the Mid‐Pleistocene Transition. Vegetation restructuring from forest into tundra could have confined these forest species into isolated glacial macro‐ and microrefugia. During subsequent warming periods, range expansions of these species could have been hampered by apomictic derivatives and by other congeneric competitors in the same habitat."],["dc.description.sponsorship","German Research Foundation http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/mec.15458"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81617"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1365-294X"],["dc.relation.issn","0962-1083"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Phylogenomics unravels Quaternary vicariance and allopatric speciation patterns in temperate‐montane plant species: A case study on the Ranunculus auricomus species complex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","nph.18284"],["dc.bibliographiccitation.journal","New Phytologist"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Wagner, Natascha"],["dc.contributor.author","Marinček, Pia"],["dc.contributor.author","Barke, Birthe Hilkka"],["dc.contributor.author","Paetzold, Claudia"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2022-06-01T09:39:31Z"],["dc.date.available","2022-06-01T09:39:31Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1111/nph.18284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/108499"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-572"],["dc.relation.eissn","1469-8137"],["dc.relation.issn","0028-646X"],["dc.rights.uri","http://onlinelibrary.wiley.com/termsAndConditions#vor"],["dc.title","Untying Gordian knots: Unraveling reticulate polyploid plant evolution by genomic data using the large Ranunculus auricomus species complex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","14066"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Ecology and Evolution"],["dc.bibliographiccitation.lastpage","14084"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Prinz, Kathleen"],["dc.date.accessioned","2020-12-10T14:06:04Z"],["dc.date.available","2020-12-10T14:06:04Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1002/ece3.5844"],["dc.identifier.eissn","2045-7758"],["dc.identifier.issn","2045-7758"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/17035"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/69767"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Desert‐like badlands and surrounding (semi‐)dry grasslands of Central Germany promote small‐scale phenotypic and genetic differentiation in Thymus praecox"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2659"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Molecular Ecology"],["dc.bibliographiccitation.lastpage","2675"],["dc.bibliographiccitation.volume","30"],["dc.contributor.affiliation","Tomasello, Salvatore; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.affiliation","Hodač, Ladislav; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.affiliation","Lorberg, Ellen; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.affiliation","Daubert, Mareike; 1Department of Systematics, Biodiversity and Evolution of Plants (with Herbarium) Albrecht‐von‐Haller Institute for Plant Sciences University of Göttingen Göttingen Germany"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Lorberg, Ellen"],["dc.contributor.author","Daubert, Mareike"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-06-01T09:42:03Z"],["dc.date.available","2021-06-01T09:42:03Z"],["dc.date.issued","2021"],["dc.date.updated","2022-03-21T06:14:46Z"],["dc.description.abstract","Abstract Geographical parthenogenesis (GP) describes the phenomenon that apomicts tend to have larger distribution areas and/or occur at higher altitudes or latitudes compared to sexual relatives. However, the complex effects of genome‐wide heterozygosity, ploidy, reproduction mode (sexual vs. apomictic), and environment shaping GP of plants are still not well understood. We ascertained ploidy and reproduction mode by flow cytometry of 221 populations, and added genomic RADseq data (maximum 33,165 loci) of 80 taxa of the Ranunculus auricomus polyploid plant complex in temperate Europe. We observed 7% mainly diploid sexual, 28% facultative apomictic (mean sexuality 7.1%), and 65% obligate apomictic populations. Sexuals occupied a more southern, smaller distribution area, whereas apomicts expanded their range to higher latitudes. Within the complex, we detected three main genetic clusters and highly reticulate relationships. A genetically‐informed path analysis using GLMMs revealed several significant relationships. Sexuality of populations (percent of sexual seeds) was higher in diploids compared to polyploids, associated with more petals, and similar between forests and open habitats. In contrast to other apomictic plant complexes, sexuality was mainly positively correlated to solar radiation and isothermality, which fits the southern distribution. We found up to three times higher heterozygosity in polyploids compared to diploids, and generally more heterozygous individuals in forests compared with open habitats. Interestingly, we revealed a previously unknown positive association between heterozygosity and temperature seasonality, suggesting a higher resistance of polyploids to more extreme climatic conditions. We provide empirical evidence for intrinsic and extrinsic factors shaping the GP pattern in a polyploid plant complex."],["dc.description.sponsorship","German Research Foundation http://dx.doi.org/10.13039/501100001659"],["dc.identifier.doi","10.1111/mec.15919"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/85129"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","1365-294X"],["dc.relation.issn","0962-1083"],["dc.rights","This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited."],["dc.title","Moving beyond assumptions: Polyploidy and environmental effects explain a geographical parthenogenesis scenario in European plants"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1191"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","TAXON"],["dc.bibliographiccitation.lastpage","1220"],["dc.bibliographiccitation.volume","69"],["dc.contributor.author","Karbstein, Kevin"],["dc.contributor.author","Tomasello, Salvatore"],["dc.contributor.author","Hodač, Ladislav"],["dc.contributor.author","Dunkel, Franz G."],["dc.contributor.author","Daubert, Mareike"],["dc.contributor.author","Hörandl, Elvira"],["dc.date.accessioned","2021-04-14T08:32:17Z"],["dc.date.available","2021-04-14T08:32:17Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1002/tax.12365"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83872"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1996-8175"],["dc.relation.issn","0040-0262"],["dc.title","Phylogenomics supported by geometric morphometrics reveals delimitation of sexual species within the polyploid apomictic Ranunculus auricomus complex (Ranunculaceae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]