Mannan, Ashraf U.

[["dc.bibliographiccitation.firstpage","788"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biology of Reproduction"],["dc.bibliographiccitation.lastpage","796"],["dc.bibliographiccitation.volume","69"],["dc.contributor.author","Mannan, Ashraf U."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Mueller, C."],["dc.contributor.author","Burfeind, Peter"],["dc.contributor.author","Adham, Ibrahim M."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:36:20Z"],["dc.date.available","2018-11-07T10:36:20Z"],["dc.date.issued","2003"],["dc.description.abstract","The testicular haploid expressed gene (Theg) encodes for a novel similar to42.0-kDa nuclear protein, which is specifically expressed in spermatid cells. Its expression is upregulated by some unknown factor(s) from Sertoli cells. To elucidate the function of Theg protein and its role in spermatogenesis, we disrupted the Theg locus in mouse by homologous recombination. For functional dissection of the domain structure of the Theg protein, two different knockout approaches were undertaken. In the first knockout mouse (Th14), the C-terminal region of the Theg protein (amino acids 137-376) was deleted. Both Th14(+/-) and Th14(-/-) mice from genetic backgrounds of C57BL/6J X 129X1/ SvJ hybrid and 129X1/SvJ inbred exhibited a normal phenotype and were fertile. The testes of Th14(-/-) mice were smaller than those of Th14(+/-) and Th14(+/+) mice; however, the testicular morphology and the properties of sperm, including morphology and motility, from Th14(-/-) mice were similar to those of Th14(+/-) and Th14(+/+) mice. These results demonstrate that the C-terminal region of Theg (amino acids 137-376) does not play an important role in progression of spermatogenesis. In the second knockout mouse (Th15), we deleted the N-terminal domain of the Theg protein, which resulted in complete loss of Theg transcripts. Both Th15(+/-) and Th15(-/-) mice from genetic backgrounds C57BL/6J x 129X1/SvJ hybrid, C3H/J congenic, and 129X1/SvJ inbred appeared normal and were fertile, with no gross abnormalities detected in testicular morphology or sperm properties. Our results from both knockout mouse model systems clearly illustrate that Theg is not essential for spermatogenesis in the mouse."],["dc.identifier.doi","10.1095/biolreprod.103.017400"],["dc.identifier.isi","000184989100007"],["dc.identifier.pmid","12748127"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45299"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Study Reproduction"],["dc.relation.issn","0006-3363"],["dc.title","Male mice lacking the theg (Testicular haploid expressed gene) protein undergo normal spermatogenesis and are fertile"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","229"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Neurogenetics"],["dc.bibliographiccitation.lastpage","238"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Mannan, Ashraf U."],["dc.contributor.author","Roussa, Eleni"],["dc.contributor.author","Kraus, Cornelia"],["dc.contributor.author","Rickmann, Michael"],["dc.contributor.author","Maenner, Joerg"],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Krieglstein, Kerstin"],["dc.contributor.author","Reis, A."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:43:34Z"],["dc.date.available","2018-11-07T10:43:34Z"],["dc.date.issued","2004"],["dc.description.abstract","We report a novel spontaneous mutation named nax in mice, which exhibit delayed hair appearance and ataxia in a homozygote state. Histological analyses of nax brain revealed an overall impairment of the cerebellar cortex. The classical cortical cytoarchitecture was disrupted, the inner granule cell layer was not obvious, the Purkinje cells were not aligned as a Purkinje cell layer, and Bergmann glias did not span the molecular layer. Furthermore, histological analyses of skin showed that the hair follicles were also abnormal. We mapped the nax locus between marker D2Mit158 and D2Mit100 within a region of 800 kb in the middle of chromosome 2 and identified a missense mutation (Gly244Glu) in Acp2, a lysosomal monoesterase. The Glu244 mutation does not affect the stability of the Acp2 transcript, however it renders the enzyme inactive. Ultrastructural analysis of nax cerebellum showed lysosomal storage bodies in nucleated cells, suggesting progressive degeneration as the underlying mechanism. Identification of Acp2 as the gene mutated in nax mice provides a valuable model system for studying the role of Acp2 in cerebellum and skin homeostasis."],["dc.identifier.doi","10.1007/s10048-004-0197-9"],["dc.identifier.isi","000226285100005"],["dc.identifier.pmid","15503243"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47086"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1364-6745"],["dc.title","Mutation in the gene encoding lysosomal acid phosphatase (Acp2) causes cerebellum and skin malformation in mouse"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","431"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Molecular Reproduction and Development"],["dc.bibliographiccitation.lastpage","438"],["dc.bibliographiccitation.volume","66"],["dc.contributor.author","Mannan, Ashraf U."],["dc.contributor.author","Nica, G."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Mueller, C."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:34:29Z"],["dc.date.available","2018-11-07T10:34:29Z"],["dc.date.issued","2003"],["dc.description.abstract","The murine calgizzarin like gene (Cal) encodes for a calcium binding protein, which belongs to the S100 family of EF-hand proteins. It is specifically expressed in Sertoli cells in the testis and its expression is down-regulated by unknown factor(s) from spermatocytes/spermatids. In this paper, we show by transfection of a fusion protein of green fluorescent protein and Cal protein into NIH3T3 cells, that the expression of Cal is restricted only in the cytoplasm of the cell. A differentially regulated cytoplasmic expression of the Cal in Sertoli cells during mouse development suggests that Cal might play an important role during spermatogenesis. In order to elucidate the function of the Cal protein in the spermatogenesis, we disrupted the Cal locus in mouse by homologous recombination. In our knockout mouse, we deleted exon 2 and exon 3 of the Cal gene and replaced them with a neomycin cassette, which resulted in a complete loss of the Cal transcript. Male and female Cal4(+/-) and Cal4(-/-) mice from genetic backgrounds C57BL/6J x 129X1/SvJ hybrid and 129X1/SvJ inbred exhibited normal phenotype and were fertile. An intensive phenotypic analysis showed no gross abnormalities in testis morphology. The lack of the Cal protein also does not affect the parameters of sperm, as they are able to fertilize the oocytes in a competent manner, which is comparable to wild-type sperm. Collectively our results demonstrate that Cal is a nonessential protein and it does not play an important role in mouse spermatogenesis or in process of fertilization. (C) 2003 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/mrd.10367"],["dc.identifier.isi","000186391400012"],["dc.identifier.pmid","14579419"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44884"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","1040-452X"],["dc.title","Calgizarrin like gene (Cal) deficient mice undergo normal spermatogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Journal of Human Genetics"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Mannan, Ashraf U."],["dc.contributor.author","Nica, G."],["dc.contributor.author","Nayernia, K."],["dc.contributor.author","Engel, Wolfgang"],["dc.date.accessioned","2018-11-07T10:30:08Z"],["dc.date.available","2018-11-07T10:30:08Z"],["dc.date.issued","2002"],["dc.format.extent","165"],["dc.identifier.isi","000187166100521"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43798"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","London"],["dc.relation.conference","European-Society-of-Human-Genetics European Human Genetics Conference in Conjuction With European Meeting on Psychosocial Aspects of Genetics"],["dc.relation.eventlocation","STRASBOURG, FRANCE"],["dc.relation.issn","1018-4813"],["dc.title","Sertoli cell-germ cell interaction: Functional analysis of murine calgizzarin gene."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]