Förster, Agnes

[["dc.bibliographiccitation.firstpage","161"],["dc.bibliographiccitation.journal","European Cells and Materials"],["dc.bibliographiccitation.lastpage","169"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Schliephake, H"],["dc.contributor.author","Strecker, N"],["dc.contributor.author","Förster, A"],["dc.contributor.author","Schwenzer, B"],["dc.contributor.author","Reichert, J"],["dc.contributor.author","Scharnweber, D"],["dc.date.accessioned","2021-06-01T10:48:38Z"],["dc.date.available","2021-06-01T10:48:38Z"],["dc.date.issued","2012"],["dc.description.abstract","The aim of the present study was to test the hypothesis that sandblasted and acid etched titanium surfaces can be functionalised with vascular endothelial growth factor (VEGF) using oligonucleotides for anchorage and slow release. rhVEGF165 molecules were conjugated to strands of 30-mer non-coding DNA oligonucleotides (ODN) and hybridised to complementary ODN anchor strands which had been immobilised to the surface of sandblasted/acid etched (SAE) Ti specimens. Specimens with non-conjugated VEGF adsorbed to ODN anchor strands and to blank SAE surfaces served as controls. Specifi c binding of conjugated VEGF exhibited the highest percentage of immobilised VEGF (71.0 %), whereas non-conjugated VEGF only achieved 53.2 and 30.7 %, respectively. Cumulative release reached 54.0 % of the immobilised growth factor in the group of specifi cally bound VEGF after 4 weeks, whereas non-conjugated VEGF adsorbed to ODN strands released 78.9 % and VEGF adsorbed to SAE Ti surfaces released 97.4 %. Proliferation of human umbilical vein endothelial cells (HUVECs) was signifi cantly increased on the surfaces with specifi cally bound VEGF compared to the control surfaces and SAE Ti surfaces without VEGF. Moreover, the released conjugated VEGF exhibited biological activity by induction of von Willebrand Factor (vWF) in mesenchymal stem cells. It is concluded that the angiogenic functionalisation of SAE titanium surfaces can be achieved by conjugation of VEGF to ODN strands and hybridisation to complementary ODN strands that are anchored to the titanium surface. The angiogenic effect is exerted both through the immobilised and the released portion of the growth factor."],["dc.identifier.doi","10.22203/eCM.v023a12"],["dc.identifier.fs","585800"],["dc.identifier.pmid","22415802"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/9549"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86003"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1473-2262"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Angiogenic functionalisation of titanium surfaces using nano-anchored VEGF – an in vitro study"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","28"],["dc.bibliographiccitation.journal","European Cells and Materials"],["dc.bibliographiccitation.lastpage","40"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Schliephake, H"],["dc.contributor.author","Rublack, J"],["dc.contributor.author","Aeckerle, N"],["dc.contributor.author","Förster, A"],["dc.contributor.author","Schwenzer, B"],["dc.contributor.author","Reichert, J"],["dc.contributor.author","Scharnweber, D"],["dc.contributor.authorgroup","Dept. of Oral and Maxillofacial Surgery, George-Augusta-University, Robert-Koch-Str 40, 37075 Göttingen, Germany"],["dc.date.accessioned","2021-06-01T10:48:38Z"],["dc.date.available","2021-06-01T10:48:38Z"],["dc.date.issued","2015"],["dc.description.abstract","The aim of the present study was to test the hypothesis that immobilisation of bone morphogenic proteins on the surface of titanium implants through nano-anchored oligonucleotides can enhance peri-implant bone formation. Non-coding 60-mer DNA oligonucleotides (ODN) were anchored to the surface of custom made sandblasted acid etched (SAE) titanium screw implants through anodic polarisation, gamma-sterilised with a standard dose of 25 kGy, and were hybridised with complementary 30-mer strands of DNA oligonucleotides conjugated to rhBMP2. Blank SAE implants, SAE implants with nano-anchored ODN and SAE implants with nano-anchored ODN and non-conjugated rhBMP2 served as controls. The implants were inserted into the tibiae of 36 Sprague Dawley rats. Perforations at the head and the tip of the implants allowed for bone ingrowth. Bone ingrowth into perforations and bone implant contact (BIC) as well as bone density (BD) at a distance of 200 mu m from the implant surface were assessed after 1, 4 and 13 weeks. Implants with nano-anchored ODN strands hybridised with conjugated rhBMP2 exhibited enhanced bone ingrowth into the perforations and increased BIC after 1 week as well as increased BIC after 4 weeks compared to controls. No difference was seen after 13 weeks. Bone density around the outer implant surface did not differ significantly at any of the intervals. It is concluded that rhBMP2 immobilised on the surface of titanium implants through nano-anchored oligonucleotide strands can enhance bone implant contact. The conditions of sterilisation tested allowed for handling under clinically relevant conditions."],["dc.identifier.doi","10.22203/eCM.v030a03"],["dc.identifier.isi","000366141600003"],["dc.identifier.pmid","26214288"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86005"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Ao Research Institute Davos-ari"],["dc.relation.issn","1473-2262"],["dc.title","In vivo effect of immobilisation of bone morphogenic protein 2 on titanium implants through nano-anchored oligonucleotides"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]