Neher, Erwin

[["dc.bibliographiccitation.firstpage","3980"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Neuron"],["dc.bibliographiccitation.lastpage","4000.e7"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Lipstein, Noa"],["dc.contributor.author","Chang, Shuwen"],["dc.contributor.author","Lin, Kun-Han"],["dc.contributor.author","López-Murcia, Francisco José"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Taschenberger, Holger"],["dc.contributor.author","Brose, Nils"],["dc.date.accessioned","2022-01-11T14:05:37Z"],["dc.date.available","2022-01-11T14:05:37Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.neuron.2021.09.054"],["dc.identifier.pii","S0896627321007273"],["dc.identifier.pmid","34706220"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/97705"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/401"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/139"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-507"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation.issn","0896-6273"],["dc.relation.workinggroup","RG Brose"],["dc.relation.workinggroup","RG Neher (Membrane Biophysics)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Munc13-1 is a Ca2+-phospholipid-dependent vesicle priming hub that shapes synaptic short-term plasticity and enables sustained neurotransmission"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","182"],["dc.bibliographiccitation.journal","Neuroscience"],["dc.bibliographiccitation.lastpage","202"],["dc.bibliographiccitation.volume","458"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Taschenberger, Holger"],["dc.date.accessioned","2021-04-14T08:28:52Z"],["dc.date.available","2021-04-14T08:28:52Z"],["dc.date.issued","2021"],["dc.description.abstract","Synaptic vesicles (SVs) undergo multiple steps of functional maturation (priming) before being fusion competent. We present an analysis technique, which decomposes the time course of quantal release during repetitive stimulation as a sum of contributions of SVs, which existed in distinct functional states prior to stimulation. Such states may represent different degrees of maturation in priming or relate to different molecular composition of the release apparatus. We apply the method to rat calyx of Held synapses. These synapses display a high degree of variability, both with respect to synaptic strength and short-term plasticity during high-frequency stimulus trains. The method successfully describes time courses of quantal release at individual synapses as linear combinations of three components, representing contributions from functionally distinct SV subpools, with variability among synapses largely covered by differences in subpool sizes. Assuming that SVs transit in sequence through at least two priming steps before being released by an action potential (AP) we interpret the components as representing SVs which had been ‘fully primed’, ‘incompletely primed’ or undocked prior to stimulation. Given these assumptions, the analysis reports an initial release probability of 0.43 for SVs that were fully primed prior to stimulation. Release probability of that component was found to increase during high-frequency stimulation, leading to rapid depletion of that subpool. SVs that were incompletely primed at rest rapidly obtain fusion-competence during repetitive stimulation and contribute the majority of release after 3–5 stimuli."],["dc.identifier.doi","10.1016/j.neuroscience.2020.10.012"],["dc.identifier.pmid","33454165"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82726"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/115"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/88"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation.issn","0306-4522"],["dc.relation.workinggroup","RG Neher (Membrane Biophysics)"],["dc.rights","CC BY-NC-ND 4.0"],["dc.title","Non-negative Matrix Factorization as a Tool to Distinguish Between Synaptic Vesicles in Different Functional States"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","P219"],["dc.bibliographiccitation.journal","BMC Neuroscience"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Bibitchkov, Dmitry"],["dc.contributor.author","Taschenberger, Holger"],["dc.contributor.author","Neher, Erwin"],["dc.date.accessioned","2010-11-25T19:28:52Z"],["dc.date.accessioned","2011-06-29T15:42:22Z"],["dc.date.accessioned","2021-10-27T13:11:08Z"],["dc.date.available","2010-11-25T19:28:52Z"],["dc.date.available","2011-06-29T15:42:22Z"],["dc.date.available","2021-10-27T13:11:08Z"],["dc.date.issued","2009"],["dc.date.updated","2010-11-25T19:28:52Z"],["dc.description.abstract","The calyx of Held is a giant synaptic terminal in the MNTB of the auditory brainstem. Observations of postsynaptic responses to repeated stimulation reveal that this synapse exhibits complex behavior, which results from the interplay between processes such as vesicle pool depletion, activity-dependent recovery from depletion, synaptic facilitation and postsynaptic receptor desensitization. Intracellular calcium concentration ([Ca2+]) plays an important role in the neurotransmitter release and the regulation of short-term plasticity [1]. We build a detailed computational model of the synaptic transmission at the calyx, which captures all the above mentioned processes and includes the dynamics of calcium and its influence on the parameters of synaptic dynamics. The model is based on electrophysiological data obtained by patch clamp recordings from postsynaptic MNTB cells in rat brainstem slices. Calcium dynamics and the functional dependences of synaptic parameters on [Ca2+] are modeled based on previously published data [2]. Stimulating the synapse at different frequencies (from 0.2 to 200 Hz) and using pharmacological manipulations, we were able to separate contributions of different processes and determine the parameters of synaptic dynamics, which fit experimental data. We compare the fit with the previous phenomenological model [3], which does not include explicit dynamics of intracellular Ca2+."],["dc.identifier.doi","10.1186/1471-2202-10-S1-P219"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/91564"],["dc.language.iso","en"],["dc.language.rfc3066","en"],["dc.notes.intern","Migrated from goescholar"],["dc.relation.orgunit","Fakultät für Biologie und Psychologie"],["dc.rights","CC BY 2.0"],["dc.rights.holder","et al.; licensee BioMed Central Ltd."],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.subject.ddc","570"],["dc.title","Model of synaptic transmission in the calyx of Held"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]