Hotop, Andrea

[["dc.bibliographiccitation.firstpage","865"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Clinical and Vaccine Immunology"],["dc.bibliographiccitation.lastpage","874"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Maksimov, Pavlo"],["dc.contributor.author","Zerweck, Johannes"],["dc.contributor.author","Maksimov, Aline"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Pleyer, Uwe"],["dc.contributor.author","Spekker, Katrin"],["dc.contributor.author","Daeubener, Walter"],["dc.contributor.author","Werdermann, Sandra"],["dc.contributor.author","Niederstrasser, Olaf"],["dc.contributor.author","Petri, Eckhardt"],["dc.contributor.author","Mertens, Marc"],["dc.contributor.author","Ulrich, Rainer G."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T09:09:27Z"],["dc.date.available","2018-11-07T09:09:27Z"],["dc.date.issued","2012"],["dc.description.abstract","Toxoplasma gondii infections occur worldwide in humans and animals. In immunocompromised or prenatally infected humans, T. gondii can cause severe clinical symptoms. The identification of specific epitopes on T. gondii antigens is essential for the improvement and standardization of the serological diagnosis of toxoplasmosis. We selected 20 peptides mimicking linear epitopes on GRA1, GRA2, GRA4, and MIC3 antigenic T. gondii proteins in silico using the software ABCpred. A further 18 peptides representing previously published epitopes derived from GRA1, SAG1, NTPase1, and NTPase2 antigens were added to the panel. A peptide microarray assay was established to prove the diagnostic performance of the selected peptides with human serum samples. Seropositive human serum samples (n = 184) were collected from patients presenting with acute toxoplasmosis (n = 21), latent T. gondii infection (n = 53), and inactive ocular toxoplasmosis (n = 10) and from seropositive forest workers (n = 100). To adjust the cutoff values for each peptide, sera from seronegative forest workers (n = 75) and patients (n = 65) were used. Univariate logistic regression suggested the significant diagnostic potential of eight novel and two previously published peptides. A test based on these peptides had an overall diagnostic sensitivity of 69% (100% in ocular toxoplasmosis patients, 86% in acutely infected patients, 81% in latently infected patients, and 57% in seropositive forest workers). The analysis of seronegative sera performed with these peptides revealed a diagnostic specificity of 84%. The results of our study suggest that the use of a bioinformatic approach for epitope prediction in combination with peptide microarray testing is a powerful method for the selection of T. gondii epitopes as candidate antigens for serological diagnosis."],["dc.identifier.doi","10.1128/CVI.00119-12"],["dc.identifier.isi","000307110500006"],["dc.identifier.pmid","22496494"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26264"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1556-6811"],["dc.title","Peptide Microarray Analysis of In Silico-Predicted Epitopes for Serological Diagnosis of Toxoplasma gondii Infection in Humans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","911"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","916"],["dc.bibliographiccitation.volume","304"],["dc.contributor.author","Herrmann, Daland C."],["dc.contributor.author","Maksimov, Pavlo"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Daeubener, Walter"],["dc.contributor.author","Liesenfeld, Oliver"],["dc.contributor.author","Pleyer, Uwe"],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T09:33:58Z"],["dc.date.available","2018-11-07T09:33:58Z"],["dc.date.issued","2014"],["dc.description.abstract","Toxoplasmosis is an important zoonosis transmitted from animals to humans world-wide. In order to determine Toxoplasma gondii genotypes in individuals living in Germany and to compare findings with those in animals, we analysed nine independent and unlinked genetic markers (nSAG2, SAG3, BTUS, GRA6, c22-8, c29-2, 1358, PI(I and Apico) by PCR-RFLP in 83 archived T. gondii-positive DNA samples from patients with ocular toxoplasmosis (n=35), toxoplasmic encephalitis (n=32), systemic toxoplasmosis after bone-marrow transplantation (n = 15) and congenital toxoplasmosis (n = 1). In 46 of these 83 samples the presence of T. gondii DNA was confirmed by conventional end-point PCR. Among these, 17 T. gondii-positive samples were typed at all nine loci. The majority (15/17, 88.2%) of these samples were of T. gondii type II (i.e., including both, the Apico type II and Apico type I variants). In addition, in one sample a T. gondii type II/type III allele combination and in another sample a T. gondii genotype displaying type III alleles at all markers was observed. In the remaining 11 samples, in which T. gondii could only be partially typed, exclusively type II (n =10) or type III (n =1) alleles were observed. Results of the present study suggest that the majority of patients in Germany are infected with type II T. gondii regardless of the clinical manifestation of toxoplasmosis. This finding is in accord with the predominance of type II T. gondii in oocysts isolated from cats and in tissues of other intermediate hosts in Germany. (C) 2014 Elsevier GmbH. All rights reserved."],["dc.identifier.doi","10.1016/j.ijmm.2014.06.008"],["dc.identifier.isi","000344827300016"],["dc.identifier.pmid","25037927"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32081"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","1618-0607"],["dc.relation.issn","1438-4221"],["dc.title","Genotyping of samples from German patients with ocular, cerebral and systemic toxoplasmosis reveals a predominance of Toxoplasma gondii type II"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]