Hotop, Andrea

[["dc.bibliographiccitation.firstpage","1473"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Parasitology Research"],["dc.bibliographiccitation.lastpage","1480"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Buschtoens, Susanne"],["dc.contributor.author","Bangoura, Berit"],["dc.contributor.author","Zoeller, Birte"],["dc.contributor.author","Koethe, Martin"],["dc.contributor.author","Spekker-Bosker, Katrin"],["dc.contributor.author","Hotop, Sven-Kevin"],["dc.contributor.author","Tenter, Astrid M."],["dc.contributor.author","Daeubener, Walter"],["dc.contributor.author","Straubinger, Reinhard K."],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T09:42:16Z"],["dc.date.available","2018-11-07T09:42:16Z"],["dc.date.issued","2014"],["dc.description.abstract","Toxoplasma gondii is a parasite which can be transmitted to humans via the consumption of contaminated meat products derived from different animal species, e.g., poultry. In Europe, the consumption rate of poultry meat is high and may pose a risk for humans. However, little is known about the prevalence and immune response against T. gondii in these animals. Based on these circumstances, we experimentally infected 18 turkeys and 16 chickens with the parasite. Turkeys were infected either with tachyzoites on different routes or with various amounts of oocysts. In contrast, chickens were only infected with different doses of oocysts. The immunoglobulin (Ig) Y humoral immune responses of these animals were investigated in a lineblot assay against the recombinant T. gondii antigens rGRA1, rGRA6, rGRA9, rSAG1, and rSUB1. By using the recombinant antigens rGRA6, rGRA9, and rSUB1 in the lineblot assay, we found a correlation between the humoral immune response and the parasite stage in turkeys. Thereby, an infection with oocysts induced a stronger, permanent long-lasting antibody response compared to tachyzoite-infected animals. Only a minor relation between the oocyst infection dose and the manifestation of the immune response in chickens was found 7 days post infection (dpi) by using rGRA1 and rGRA9. However, an inconstant detection of antigen-specific IgY antibodies in the lineblot assay seems not to be a sufficient method for the identification of a Toxoplasma infection in chickens. In contrast, the detection of anti-rGRA6, anti-rGRA9, and anti-rSUB1 IgY antibodies showed potential for the identification of an infection in turkeys."],["dc.identifier.doi","10.1007/s00436-014-3788-x"],["dc.identifier.isi","000333029800025"],["dc.identifier.pmid","24532010"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33917"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-1955"],["dc.relation.issn","0932-0113"],["dc.title","Humoral immune responses in chickens and turkeys after infection with Toxoplasma gondii by using recombinant antigens"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","865"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Clinical and Vaccine Immunology"],["dc.bibliographiccitation.lastpage","874"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Maksimov, Pavlo"],["dc.contributor.author","Zerweck, Johannes"],["dc.contributor.author","Maksimov, Aline"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Pleyer, Uwe"],["dc.contributor.author","Spekker, Katrin"],["dc.contributor.author","Daeubener, Walter"],["dc.contributor.author","Werdermann, Sandra"],["dc.contributor.author","Niederstrasser, Olaf"],["dc.contributor.author","Petri, Eckhardt"],["dc.contributor.author","Mertens, Marc"],["dc.contributor.author","Ulrich, Rainer G."],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T09:09:27Z"],["dc.date.available","2018-11-07T09:09:27Z"],["dc.date.issued","2012"],["dc.description.abstract","Toxoplasma gondii infections occur worldwide in humans and animals. In immunocompromised or prenatally infected humans, T. gondii can cause severe clinical symptoms. The identification of specific epitopes on T. gondii antigens is essential for the improvement and standardization of the serological diagnosis of toxoplasmosis. We selected 20 peptides mimicking linear epitopes on GRA1, GRA2, GRA4, and MIC3 antigenic T. gondii proteins in silico using the software ABCpred. A further 18 peptides representing previously published epitopes derived from GRA1, SAG1, NTPase1, and NTPase2 antigens were added to the panel. A peptide microarray assay was established to prove the diagnostic performance of the selected peptides with human serum samples. Seropositive human serum samples (n = 184) were collected from patients presenting with acute toxoplasmosis (n = 21), latent T. gondii infection (n = 53), and inactive ocular toxoplasmosis (n = 10) and from seropositive forest workers (n = 100). To adjust the cutoff values for each peptide, sera from seronegative forest workers (n = 75) and patients (n = 65) were used. Univariate logistic regression suggested the significant diagnostic potential of eight novel and two previously published peptides. A test based on these peptides had an overall diagnostic sensitivity of 69% (100% in ocular toxoplasmosis patients, 86% in acutely infected patients, 81% in latently infected patients, and 57% in seropositive forest workers). The analysis of seronegative sera performed with these peptides revealed a diagnostic specificity of 84%. The results of our study suggest that the use of a bioinformatic approach for epitope prediction in combination with peptide microarray testing is a powerful method for the selection of T. gondii epitopes as candidate antigens for serological diagnosis."],["dc.identifier.doi","10.1128/CVI.00119-12"],["dc.identifier.isi","000307110500006"],["dc.identifier.pmid","22496494"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26264"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1556-6811"],["dc.title","Peptide Microarray Analysis of In Silico-Predicted Epitopes for Serological Diagnosis of Toxoplasma gondii Infection in Humans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.volume","302"],["dc.contributor.author","Nau, J."],["dc.contributor.author","Wenning, J."],["dc.contributor.author","Leineweber, M."],["dc.contributor.author","Spekker, Katrin"],["dc.contributor.author","Schares, Gereon"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Gross, U."],["dc.contributor.author","Daeubener, Walter"],["dc.date.accessioned","2018-11-07T09:06:04Z"],["dc.date.available","2018-11-07T09:06:04Z"],["dc.date.issued","2012"],["dc.format.extent","43"],["dc.identifier.isi","000311593300150"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25470"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.publisher.place","Jena"],["dc.relation.conference","64th Annual Meeting of the German-Society-for-Hygiene-and-Microbiology (DGHM)"],["dc.relation.eventlocation","Hamburg, GERMANY"],["dc.relation.issn","1438-4221"],["dc.title","Immunologic relevance of the secretory antigens of Toxoplasma gondii as target structures for B- and T- cell responses"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1545"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Clinical Infectious Diseases"],["dc.bibliographiccitation.lastpage","1552"],["dc.bibliographiccitation.volume","54"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Hlobil, Harald"],["dc.contributor.author","Gross, Uwe"],["dc.date.accessioned","2018-11-07T09:10:00Z"],["dc.date.available","2018-11-07T09:10:00Z"],["dc.date.issued","2012"],["dc.description.abstract","Background. Treatment of Toxoplasma gondii infection acquired during pregnancy differs in many countries. In Germany, spiramycin is given until the 16th week of pregnancy, followed by at least 4 weeks of combination therapy with pyrimethamine, sulfadiazine, and folinic acid independent of the infection stage of the fetus. If infection of the fetus is confirmed by polymerase chain reaction or if fetal ultrasound indicates severe symptoms ( hydrocephalus, ventricular dilation), treatment is continued until delivery with regularmonitoring of pyrimethamine and sulfadiazine concentration in maternal blood and observation of possible adverse effects. In other European countries, such as France, only spiramycin is given unless infection of the fetus is proven. Methods. To evaluate the effectiveness of the German treatment scheme, a retrospective analysis of 685 women who showed a serological constellation consistent with primary infection in pregnancy and their children was performed. Results. We found an increased transmission rate to the fetus with increased time in gestation and a decreased risk of clinical manifestations. In comparison with studies performed in other countries, the overall transmission rate (4.8%) and the rate of clinical manifestations in newborns (1.6%) were lower. Conclusions. Use of spiramycin from time of diagnosis of acute acquisition of infection by the pregnant woman until week 16, followed by pyrimethamine, sulfadiazine, and folinic acid for at least 4 weeks in combination with a standardized follow-up program is efficient in reducing transplacental transmission of the parasite and the burden of disease in the newborn."],["dc.identifier.doi","10.1093/cid/cis234"],["dc.identifier.isi","000304048400005"],["dc.identifier.pmid","22460980"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26395"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press Inc"],["dc.relation.issn","1058-4838"],["dc.title","Efficacy of Rapid Treatment Initiation Following Primary Toxoplasma gondii Infection During Pregnancy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","911"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","International Journal of Medical Microbiology"],["dc.bibliographiccitation.lastpage","916"],["dc.bibliographiccitation.volume","304"],["dc.contributor.author","Herrmann, Daland C."],["dc.contributor.author","Maksimov, Pavlo"],["dc.contributor.author","Hotop, Andrea"],["dc.contributor.author","Gross, Uwe"],["dc.contributor.author","Daeubener, Walter"],["dc.contributor.author","Liesenfeld, Oliver"],["dc.contributor.author","Pleyer, Uwe"],["dc.contributor.author","Conraths, Franz J."],["dc.contributor.author","Schares, Gereon"],["dc.date.accessioned","2018-11-07T09:33:58Z"],["dc.date.available","2018-11-07T09:33:58Z"],["dc.date.issued","2014"],["dc.description.abstract","Toxoplasmosis is an important zoonosis transmitted from animals to humans world-wide. In order to determine Toxoplasma gondii genotypes in individuals living in Germany and to compare findings with those in animals, we analysed nine independent and unlinked genetic markers (nSAG2, SAG3, BTUS, GRA6, c22-8, c29-2, 1358, PI(I and Apico) by PCR-RFLP in 83 archived T. gondii-positive DNA samples from patients with ocular toxoplasmosis (n=35), toxoplasmic encephalitis (n=32), systemic toxoplasmosis after bone-marrow transplantation (n = 15) and congenital toxoplasmosis (n = 1). In 46 of these 83 samples the presence of T. gondii DNA was confirmed by conventional end-point PCR. Among these, 17 T. gondii-positive samples were typed at all nine loci. The majority (15/17, 88.2%) of these samples were of T. gondii type II (i.e., including both, the Apico type II and Apico type I variants). In addition, in one sample a T. gondii type II/type III allele combination and in another sample a T. gondii genotype displaying type III alleles at all markers was observed. In the remaining 11 samples, in which T. gondii could only be partially typed, exclusively type II (n =10) or type III (n =1) alleles were observed. Results of the present study suggest that the majority of patients in Germany are infected with type II T. gondii regardless of the clinical manifestation of toxoplasmosis. This finding is in accord with the predominance of type II T. gondii in oocysts isolated from cats and in tissues of other intermediate hosts in Germany. (C) 2014 Elsevier GmbH. All rights reserved."],["dc.identifier.doi","10.1016/j.ijmm.2014.06.008"],["dc.identifier.isi","000344827300016"],["dc.identifier.pmid","25037927"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32081"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Gmbh, Urban & Fischer Verlag"],["dc.relation.issn","1618-0607"],["dc.relation.issn","1438-4221"],["dc.title","Genotyping of samples from German patients with ocular, cerebral and systemic toxoplasmosis reveals a predominance of Toxoplasma gondii type II"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]