Grabner, Chad Paul

[["dc.contributor.author","Grabner, Chad Paul"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-04-29T08:36:25Z"],["dc.date.available","2021-04-29T08:36:25Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1101/2020.10.12.336503"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/84558"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/285"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.title","The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast fusion of the readily releasable pool of vesicles"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Grabner, Chad Paul"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2021-12-01T09:22:56Z"],["dc.date.available","2021-12-01T09:22:56Z"],["dc.date.issued","2021"],["dc.description.abstract","Rod photoreceptors (PRs) use ribbon synapses to transmit visual information. To signal ‘no light detected’ they release glutamate continually to activate post-synaptic receptors. When light is detected glutamate release pauses. How a rod’s individual ribbon enables this process was studied here by recording evoked changes in whole-cell membrane capacitance from wild-type and ribbonless ( Ribeye -ko) mice. Wild-type rods filled with high (10 mM) or low (0.5 mM) concentrations of the Ca 2+ -buffer EGTA created a readily releasable pool (RRP) of 87 synaptic vesicles (SVs) that emptied as a single kinetic phase with a τ<0.4 ms. The lower concentration of EGTA accelerated Ca v channel opening and facilitated release kinetics. In contrast, ribbonless rods created a much smaller RRP of 22 SVs, and they lacked Ca v channel facilitation; however, Ca 2+ channel-release coupling remained tight. These release deficits caused a sharp attenuation of rod-driven scotopic light responses. We conclude that the synaptic ribbon facilitates Ca 2+ -influx and establishes a large RRP of SVs."],["dc.identifier.doi","10.7554/eLife.63844"],["dc.identifier.pmid","34617508"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94521"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/386"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/138"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | B05: Quantitative molekulare Physiologie aktiver Zonen in Calyx-Synapsen"],["dc.relation.eissn","2050-084X"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.rights","CC BY 4.0"],["dc.title","The mammalian rod synaptic ribbon is essential for Cav channel facilitation and ultrafast synaptic vesicle fusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Jean, Philippe"],["dc.contributor.author","Lopez de la Morena, David"],["dc.contributor.author","Michanski, Susann"],["dc.contributor.author","Jaime Tobón, Lina María"],["dc.contributor.author","Gültas, Mehmet"],["dc.contributor.author","Maxeiner, Stephan"],["dc.contributor.author","Strenzke, Nicola"],["dc.contributor.author","Chakrabarti, Rituparna"],["dc.contributor.author","Picher, Maria Magdalena"],["dc.contributor.author","Neef, Jakob"],["dc.contributor.author","Jung, SangYong"],["dc.contributor.author","Neef, Andreas"],["dc.contributor.author","Wichmann, Carolin"],["dc.contributor.author","Grabner, Chad"],["dc.contributor.author","Moser, Tobias"],["dc.date.accessioned","2020-11-24T10:41:13Z"],["dc.date.available","2020-11-24T10:41:13Z"],["dc.date.issued","2018"],["dc.description.abstract","We studied the role of the synaptic ribbon for sound encoding at the synapses between inner hair cells (IHCs) and spiral ganglion neurons (SGNs) in mice lacking RIBEYE (RBEKO/KO). Electron and immunofluorescence microscopy revealed a lack of synaptic ribbons and an assembly of several small active zones (AZs) at each synaptic contact. Spontaneous and sound-evoked firing rates of SGNs and their compound action potential were reduced, indicating impaired transmission at ribbonless IHC-SGN synapses. The temporal precision of sound encoding was impaired and the recovery of SGN-firing from adaptation indicated slowed synaptic vesicle (SV) replenishment. Activation of Ca2+-channels was shifted to more depolarized potentials and exocytosis was reduced for weak depolarizations. Presynaptic Ca2+-signals showed a broader spread, compatible with the altered Ca2+-channel clustering observed by super-resolution immunofluorescence microscopy. We postulate that RIBEYE disruption is partially compensated by multi-AZ organization. The remaining synaptic deficit indicates ribbon function in SV-replenishment and Ca2+-channel regulation."],["dc.identifier.doi","10.7554/eLife.29275"],["dc.identifier.eissn","2050-084X"],["dc.identifier.pmid","29328020"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/69157"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation.issn","2050-084X"],["dc.title","The synaptic ribbon is critical for sound encoding at high rates and with temporal precision"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

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[["dc.bibliographiccitation.firstpage","3993"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","The Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","4006"],["dc.bibliographiccitation.volume","217"],["dc.contributor.author","Hagiwara, Akari"],["dc.contributor.author","Kitahara, Yosuke"],["dc.contributor.author","Grabner, Chad Paul"],["dc.contributor.author","Vogl, Christian"],["dc.contributor.author","Abe, Manabu"],["dc.contributor.author","Kitta, Ryo"],["dc.contributor.author","Ohta, Keisuke"],["dc.contributor.author","Nakamura, Keiichiro"],["dc.contributor.author","Sakimura, Kenji"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Nishi, Akinori"],["dc.contributor.author","Ohtsuka, Toshihisa"],["dc.date.accessioned","2020-12-10T18:15:36Z"],["dc.date.available","2020-12-10T18:15:36Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1083/jcb.201704076"],["dc.identifier.eissn","1540-8140"],["dc.identifier.issn","0021-9525"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74896"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","103"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Physiological Reviews"],["dc.bibliographiccitation.lastpage","144"],["dc.bibliographiccitation.volume","100"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Grabner, Chad P."],["dc.contributor.author","Schmitz, Frank"],["dc.date.accessioned","2020-12-10T18:37:40Z"],["dc.date.available","2020-12-10T18:37:40Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1152/physrev.00026.2018"],["dc.identifier.eissn","1522-1210"],["dc.identifier.issn","0031-9333"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/77057"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Sensory Processing at Ribbon Synapses in the Retina and the Cochlea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]