Opazo Davila, Luis Felipe

[["dc.bibliographiccitation.artnumber","820"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Gomes de Castro, Maria Angela"],["dc.contributor.author","Wildhagen, Hanna"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Hitzing, Christoffer"],["dc.contributor.author","Binder, Mascha"],["dc.contributor.author","Trepel, Martin"],["dc.contributor.author","Engels, Niklas"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2019-07-09T11:50:13Z"],["dc.date.available","2019-07-09T11:50:13Z"],["dc.date.issued","2019"],["dc.description.abstract","Stimulation of the B cell antigen receptor (BCR) triggers signaling pathways that promote the differentiation of B cells into plasma cells. Despite the pivotal function of BCR in B cell activation, the organization of the BCR on the surface of resting and antigen-activated B cells remains unclear. Here we show, using STED super-resolution microscopy, that IgM-containing BCRs exist predominantly as monomers and dimers in the plasma membrane of resting B cells, but form higher oligomeric clusters upon stimulation. By contrast, a chronic lymphocytic leukemia-derived BCR forms dimers and oligomers in the absence of a stimulus, but a single amino acid exchange reverts its organization to monomers in unstimulated B cells. Our super-resolution microscopy approach for quantitatively analyzing cell surface proteins may thus help reveal the nanoscale organization of immunoreceptors in various cell types."],["dc.identifier.doi","10.1038/s41467-019-08677-1"],["dc.identifier.pmid","30778055"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15884"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59725"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2041-1723"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","610"],["dc.title","Differential organization of tonic and chronic B cell antigen receptors in the plasma membrane"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","10226"],["dc.bibliographiccitation.issue","18"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.lastpage","10239"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Schlichthaerle, Thomas"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Strauss, Sebastian"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Jungmann, Ralf"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2021-04-14T08:26:44Z"],["dc.date.available","2021-04-14T08:26:44Z"],["dc.date.issued","2020"],["dc.description.abstract","A standard procedure to study cellular elements is via immunostaining followed by optical imaging. This methodology typically requires target-specific primary antibodies (1.Abs), which are revealed by secondary antibodies (2.Abs). Unfortunately, the antibody bivalency, polyclonality, and large size can result in a series of artifacts. Alternatively, small, monovalent probes, such as single-domain antibodies (nanobodies) have been suggested to minimize these limitations. The discovery and validation of nanobodies against specific targets are challenging, thus only a minimal amount of them are currently available. Here, we used STED, DNA-PAINT, and light-sheet microscopy, to demonstrate that secondary nanobodies (1) increase localization accuracy compared to 2.Abs; (2) allow direct pre-mixing with 1.Abs before staining, reducing experimental time, and enabling the use of multiple 1.Abs from the same species; (3) penetrate thick tissues more efficiently; and (4) avoid probe-induced clustering of target molecules observed with conventional 2.Abs in living or poorly fixed samples. Altogether, we show how secondary nanobodies are a valuable alternative to 2.Abs."],["dc.identifier.doi","10.1039/d0nr00227e"],["dc.identifier.pmid","32356544"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82054"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/185"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2040-3372"],["dc.relation.haserratum","/handle/2/81970"],["dc.relation.issn","2040-3364"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 3.0"],["dc.title","Circumvention of common labelling artefacts using secondary nanobodies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","48"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cells"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Oleksiievets, Nazar"],["dc.contributor.author","Isbaner, Sebastian"],["dc.contributor.author","Eggert Martínez, Mariana"],["dc.contributor.author","Enderlein, Jörg"],["dc.contributor.author","Tsukanov, Roman"],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2020-12-10T18:46:58Z"],["dc.date.available","2020-12-10T18:46:58Z"],["dc.date.issued","2019"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.3390/cells8010048"],["dc.identifier.eissn","2073-4409"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/78601"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.publisher","MDPI"],["dc.relation.eissn","2073-4409"],["dc.rights","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Nanobody Detection of Standard Fluorescent Proteins Enables Multi-Target DNA-PAINT with High Resolution and Minimal Displacement Errors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Chemical Communications"],["dc.contributor.author","Kabatas Glowacki, Selda"],["dc.contributor.author","Agüi-Gonzalez, Paola"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Jähne, Sebastian"],["dc.contributor.author","Opazo Davila, Luis Felipe"],["dc.contributor.author","Phan, Nhu T. N."],["dc.contributor.author","Rizzoli, Silvio O."],["dc.date.accessioned","2022-07-01T07:34:55Z"],["dc.date.available","2022-07-01T07:34:55Z"],["dc.date.issued","2022"],["dc.description.abstract","We developed here an iodine-containing probe that can be used to identify the molecules of interest in secondary ion mass spectrometry (SIMS) by simple immunolabelling procedures. The immunolabelled iodine probe was readily combined with previously-developed SIMS probes carrying fluorine, to generate dual-channel SIMS data. This probe should provide a useful complement to the currently available SIMS probes, thus expanding the scope of this technology."],["dc.description.sponsorship"," Deutsche Forschungsgemeinschaft https://doi.org/10.13039/501100001659"],["dc.description.sponsorship"," Volkswagen Foundation https://doi.org/10.13039/501100001663"],["dc.identifier.doi","10.1039/D2CC02290G"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112039"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/501"],["dc.identifier.url","https://sfb1286.uni-goettingen.de/literature/publications/167"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-581"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation","SFB 1286: Quantitative Synaptologie"],["dc.relation","SFB 1286 | A03: Dynamische Analyse der Remodellierung der extrazellulären Matrix (ECM) als Mechanismus der Synapsenorganisation und Plastizität"],["dc.relation.eissn","1364-548X"],["dc.relation.issn","1359-7345"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.relation.workinggroup","RG Phan"],["dc.rights","CC BY 3.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/3.0/"],["dc.title","An iodine-containing probe as a tool for molecular detection in secondary ion mass spectrometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Götzke, Hansjörg"],["dc.contributor.author","Kilisch, Markus"],["dc.contributor.author","Martínez-Carranza, Markel"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Rajavel, Abirami"],["dc.contributor.author","Schlichthaerle, Thomas"],["dc.contributor.author","Engels, Niklas"],["dc.contributor.author","Jungmann, Ralf"],["dc.contributor.author","Stenmark, Pål"],["dc.contributor.author","Opazo, Felipe"],["dc.contributor.author","Frey, Steffen"],["dc.date.accessioned","2020-12-10T18:09:51Z"],["dc.date.available","2020-12-10T18:09:51Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41467-019-12301-7"],["dc.identifier.eissn","2041-1723"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16514"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73776"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","24543"],["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Nanoscale"],["dc.bibliographiccitation.lastpage","24543"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Sograte-Idrissi, Shama"],["dc.contributor.author","Schlichthaerle, Thomas"],["dc.contributor.author","Duque-Afonso, Carlos J."],["dc.contributor.author","Alevra, Mihai"],["dc.contributor.author","Strauss, Sebastian"],["dc.contributor.author","Moser, Tobias"],["dc.contributor.author","Jungmann, Ralf"],["dc.contributor.author","Rizzoli, Silvio O."],["dc.contributor.author","Opazo, Felipe"],["dc.date.accessioned","2021-04-14T08:26:30Z"],["dc.date.available","2021-04-14T08:26:30Z"],["dc.date.issued","2020"],["dc.description.abstract","Correction for ‘Circumvention of common labelling artefacts using secondary nanobodies’ by Shama Sograte-Idrissi et al., Nanoscale, 2020, 12, 10226–10239, DOI: 10.1039/D0NR00227E."],["dc.identifier.doi","10.1039/d0nr90279a"],["dc.identifier.pmid","33306074"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/81970"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/419"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.eissn","2040-3372"],["dc.relation.iserratumof","/handle/2/82054"],["dc.relation.issn","2040-3364"],["dc.relation.workinggroup","RG Moser (Molecular Anatomy, Physiology and Pathology of Sound Encoding)"],["dc.relation.workinggroup","RG Rizzoli (Quantitative Synaptology in Space and Time)"],["dc.rights","CC BY 3.0"],["dc.title","Correction: Circumvention of common labelling artefacts using secondary nanobodies"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]