Malik, Ihtzaz Ahmed

[["dc.bibliographiccitation.journal","Laboratory Investigation"],["dc.bibliographiccitation.volume","91"],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.contributor.author","Naz, N."],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Khan, Sajjad"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T08:59:20Z"],["dc.date.available","2018-11-07T08:59:20Z"],["dc.date.issued","2011"],["dc.format.extent","368A"],["dc.identifier.isi","000291285001219"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23867"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","New york"],["dc.relation.conference","100th Annual Meeting of the United States and Canadian-Academy-of-Pathology"],["dc.relation.eventlocation","San Antonio, TX"],["dc.relation.issn","0023-6837"],["dc.title","Comparison of Changes in the Gene Expression of Transferrin Receptor-1 and Other Iron Regulatory Proteins in the Rat Liver and Brain during Acute-Phase-Response"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","842"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Laboratory Investigation"],["dc.bibliographiccitation.lastpage","856"],["dc.bibliographiccitation.volume","92"],["dc.contributor.author","Naz, Naila"],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Ahmad, Shakil"],["dc.contributor.author","Khan, Sajjad"],["dc.contributor.author","Blaschke, Martina"],["dc.contributor.author","Schultze, Frank"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T09:09:49Z"],["dc.date.available","2018-11-07T09:09:49Z"],["dc.date.issued","2012"],["dc.description.abstract","Liver is the central organ of iron metabolism. During acute-phase-response (APR), serum iron concentration rapidly decreases. The current study aimed to compare expression and localization of iron transport protein ferroportin-1 (Fpn-1) and of other iron import proteins after experimental tissue damage induced by injecting turpentine oil in the hind limbs of rats and mice. Serum and spleen iron concentration decreased with an increase in total liver, cytoplasmic and nuclear iron concentration. In liver, mRNA amount of Fpn-1, Fpn-1a, Fpn-1b, HFE, hemojuvelin (HJV) and hephaestin (heph) genes showed a rapid decrease. Hepcidin, divalent metal transporter-1 (DMT-1), transferrin (Tf) and Tf-receptor-1 (TfR1), TfR-2 (TfR2) gene expression was increased. Western blot analysis of liver tissue lysate confirmed the changes observed at mRNA level. In spleen, a rapid decrease in gene expression of Fpn-1, Fpn-1a, Fpn-1b, DMT-1, Tf, TfR1 and TfR2, and an increase in hepcidin was observed. Immunohistochemistry of DMT-1 and TfR2 were mainly detected in the nucleus of rat liver and spleen, whereas TfR1 was clearly localized in the plasma membrane. Fpn-1 was mostly found in the nuclei of liver cells, whereas in spleen, the protein was mainly detected in the cell membrane. Western blot analysis of liver fractions confirmed immunohistochemical results. In livers of wild-type mice, gene expression of Fpn-1, Fpn-1a and Fpn-1b was downregulated, whereas hepcidin gene expression was increased. In contrast, these changes were less pronounced in IL-6ko-mice. Cytokine (IL-6, IL-1 beta and TNF-alpha) treatment of rat hepatocytes showed a downregulation of Fpn-1, Fpn-1a and Fpn-1b, and upregulation of hepcidin gene expression. Moreover, western blot analysis of cell lysate of IL-6-treated hepatocytes detected, as expected, an increase of alpha 2-macroglobulin (positive acute-phase protein), whereas albumin (negative acute-phase protein) and Fpn-1 were downregulated. Our results demonstrate that liver behaves as a 'sponge' for iron under acute-phase conditions, and Fpn-1 behaves as a negative acute-phase protein in rat hepatocytes mainly, but not exclusively, because of the effect of IL-6. These changes could explain iron retention in the cytoplasm and in the nucleus of hepatocytes during APR. Laboratory Investigation (2012) 92, 842-856; doi:10.1038/labinvest.2012.52; published online 2 April 2012"],["dc.description.sponsorship","Deutsche Krebshilfe [108774]"],["dc.identifier.doi","10.1038/labinvest.2012.52"],["dc.identifier.isi","000304730600004"],["dc.identifier.pmid","22469696"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26353"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0023-6837"],["dc.title","Ferroportin-1 is a 'nuclear'-negative acute-phase protein in rat liver: a comparison with other iron-transport proteins"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","125671"],["dc.bibliographiccitation.journal","BioMed Research International"],["dc.contributor.author","Abbasi, Muddasir Hassan"],["dc.contributor.author","Fatima, Sana"],["dc.contributor.author","Naz, Naila"],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.contributor.author","Sheikh, Nadeem"],["dc.date.accessioned","2018-11-07T09:29:39Z"],["dc.date.available","2018-11-07T09:29:39Z"],["dc.date.issued","2013"],["dc.description.abstract","To gain insight into the hepatohistological alterations in noninjured rat liver, Nerium oleander (N.O.) leaves extract was injected intramuscularly to induce an acute phase reaction (APR). Histopathological changes were studied after 3, 12, and 24 h time course of sterile muscle abscess. Tissue integrity and any infiltration of inflammatory cells in the liver were investigated by Hematoxylin and Eosin and ED1 peroxidase stainings. The administration of N.O. leaves extract (10 mL/kg) in H & E stained sections showed a general vacuolization of cytoplasm resulting loss of polarity with prominent nucleoli after 3 h of induction. At 12 h, eccentric nuclei were also observed in the sections. Marked infiltration of leucocytes with predominate macrophages was also found after 24 h as seen by ED1 positive staining. In the present study, a possible relationship between serum hepcidin and total iron level was also investigated in vivo. An early increase of hepcidin and total iron level (3h) with a maximum at 12h (P < 0.01; P < 0.001) was observed. These changes indicate that sterile muscle abscess may induce APR resulting in hepatic damage which is evident with the recruitment of inflammatory cells into the organ."],["dc.identifier.doi","10.1155/2013/125671"],["dc.identifier.isi","000324179500001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31095"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Hindawi Publishing Corporation"],["dc.relation.issn","2314-6133"],["dc.title","Effect of Nerium oleander (NO) Leaves Extract on Serum hepcidin, Total Iron, and Infiltration of ED1 Positive Cells in Albino Rat"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","The Journal of Physiological Sciences"],["dc.bibliographiccitation.volume","59"],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Abid, Rabia"],["dc.contributor.author","Qureshi, Asma W."],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.contributor.author","Basheer, Tariq"],["dc.date.accessioned","2018-11-07T08:34:57Z"],["dc.date.available","2018-11-07T08:34:57Z"],["dc.date.issued","2009"],["dc.format.extent","439"],["dc.identifier.isi","000271023103076"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17945"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Tokyo"],["dc.relation.issn","1880-6546"],["dc.title","STUDY OF EXPRESSION OF LOW MOLECULAR WEIGHT PROTEINS IN DIFFERENT AGE GROUPS OF LEUKEMIA PATIENTS"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S145"],["dc.bibliographiccitation.journal","Journal of Hepatology"],["dc.bibliographiccitation.lastpage","S146"],["dc.bibliographiccitation.volume","58"],["dc.contributor.author","Naz, N."],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.contributor.author","Ahmad, Shakil"],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T09:26:10Z"],["dc.date.available","2018-11-07T09:26:10Z"],["dc.date.issued","2013"],["dc.identifier.isi","000322983000351"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30239"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.publisher.place","Amsterdam"],["dc.relation.conference","International Liver Congress / 48th Annual Meeting of the European-Association-for-the-Study-of-the-Liver (EASL)"],["dc.relation.eventlocation","Amsterdam, NETHERLANDS"],["dc.relation.issn","0168-8278"],["dc.title","FERROPORTIN 1 IS A \"NUCLEAR\" NEGATIVE ACUTE-PHASE PROTEIN IN RAT LIVER: A COMPARISON WITH OTHER IRON-TRANSPORT PROTEINS"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1252"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Laboratory Investigation"],["dc.bibliographiccitation.lastpage","1260"],["dc.bibliographiccitation.volume","89"],["dc.contributor.author","Moriconi, Federico"],["dc.contributor.author","Ahmad, Ghayyor"],["dc.contributor.author","Ramadori, Pierluigi"],["dc.contributor.author","Malik, Ihtzaz"],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Merli, Manuela"],["dc.contributor.author","Riggio, Oliviero"],["dc.contributor.author","Dudas, Joszef"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T11:22:56Z"],["dc.date.available","2018-11-07T11:22:56Z"],["dc.date.issued","2009"],["dc.description.abstract","The liver and the spleen are the organs in which cellular material and aged erythrocytes are eliminated from the blood. Within the liver, Kupffer cells (KCs) are mainly responsible for this task, as such KCs have a pivotal role in iron metabolism. The aim of this study is to investigate the changes of hepatic gene expression in two models of KC phagocytosis. Gadolinium chloride (GD) or zymosan was injected intraperitoneally into rats and to endotoxin-resistant mice (C3H/HeJ). The animals were killed at different time points and their livers were immediately frozen in liquid nitrogen for RNA isolation and immunohistological studies. RNA was analyzed by real-time PCR and northern blot. Sera were used to measure transaminases, hepcidin and iron levels. The expression of iron metabolism genes, hepcidin, hemojuvelin (Hjv), ferroportin-1 (Fpn-1) and of the inflammatory cytokines IL-6, IL-1 beta, TNF-alpha and IFN-gamma was determined. Although phagocytosed material was detected in ED-1- and C1q-positive cells, no inflammatory cells were identified within the liver parenchyma. Serum levels of hepcidin, iron and transaminases did not differ from those of control animals. Both GD and zymosan induced an upregulation of hepcidin-gene expression in rat liver as early as 3 h, reaching a maximum 6 h after treatment. Hjv- and Fpn-1-gene expression was downregulated at the same time. IL-6 was by far the most induced acute-phase-cytokine in GD- and zymosan-treated livers, although IL-1 beta and TNF-alpha were also strongly upregulated by zymosan and to a lesser extent by GD. Similar results were obtained in the C3H/HeJ mouse strain excluding the possible role of contaminating endotoxin. This study shows that phagocytosis upregulates hepcidin-gene expression and downregulates Hjv- and Fpn-1-gene expression within the liver. These changes in iron-regulating-gene expression may be mediated by the locally produced acute-phase-cytokines. Laboratory Investigation (2009) 89, 1252-1260; doi: 10.1038/labinvest.2009.92; published online 31 August 2009"],["dc.description.sponsorship","'Deutsche Forschungsgemeinschaft' [SFB 402 TP C6, C7, D3, GRK 335]"],["dc.identifier.doi","10.1038/labinvest.2009.92"],["dc.identifier.isi","000271248200006"],["dc.identifier.pmid","19721414"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56084"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","0023-6837"],["dc.title","Phagocytosis of gadolinium chloride or zymosan induces simultaneous upregulation of hepcidin- and downregulation of hemojuvelin- and Fpn-1-gene expression in murine liver"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Modern Pathology"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.contributor.author","Naz, N."],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Khan, Sajjad"],["dc.contributor.author","Ramadori, Giuliano"],["dc.date.accessioned","2018-11-07T08:59:21Z"],["dc.date.available","2018-11-07T08:59:21Z"],["dc.date.issued","2011"],["dc.format.extent","368A"],["dc.identifier.isi","000287282302009"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/23870"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","New york"],["dc.relation.conference","100th Annual Meeting United States-and-Canadian-Academy-of-Pathology"],["dc.relation.eventlocation","San Antonio, TX"],["dc.relation.issn","0893-3952"],["dc.title","Comparison of Changes in the Gene Expression of Transferrin Receptor-1 and Other Iron Regulatory Proteins in the Rat Liver and Brain during Acute-Phase-Response."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","622"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY"],["dc.bibliographiccitation.lastpage","629"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Ahmad, Shakil"],["dc.contributor.author","Moriconi, Federico"],["dc.contributor.author","Naz, Naila"],["dc.contributor.author","Sultan, Sadaf"],["dc.contributor.author","Sheikh, Nadeem"],["dc.contributor.author","Ramadori, Giuliano"],["dc.contributor.author","Malik, Ihtzaz Ahmed"],["dc.date.accessioned","2018-11-07T09:30:00Z"],["dc.date.available","2018-11-07T09:30:00Z"],["dc.date.issued","2013"],["dc.description.abstract","Ferritin L (FTL) and Ferritin H (FTH) subunits are responsible for intercellular iron storage. We previously reported increasing amounts of liver cytoplasmic and nuclear iron content during acute phase response (APR). Aim of the present study is to demonstrate intracellular localization of ferritin subunits in liver compared with extra hepatic organs of rat under physiological and acute phase conditions. Rats were administered turpentine-oil (TO) intramuscularly to induce a sterile abscess (acute-phase-model) and sacrificed at different time points. Immunohistochemistry was performed utilizing horse-reddish-peroxidise conjugated secondary antibody on 4 mu m thick section. Liver cytoplasmic and nuclear protein were used for Western blot analysis. By means of immunohistology, FTL was detected in cytoplasm while a strong nuclear positivity for FTH was evident in the liver. Similarly, in heart, spleen and brain FTL was detected mainly in the cytoplasm while FTH demonstrated intense nuclear and a weak cytoplasmic expression. Western blot analysis of cytoplasmic and nuclear fractions from liver, heart, spleen and brain further confirmed mainly cytoplasmic expression of FTL in contrast to the nuclear and cytoplasmic expression of FTH. The data presented demonstrate the differential localization of FTL and FTH within hepatic and extra hepatic organs being FTL predominantly in the cytoplasm while FTH predominantly in nucleus."],["dc.identifier.isi","000318571700007"],["dc.identifier.pmid","23573308"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31195"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","E-century Publishing Corp"],["dc.relation.issn","1936-2625"],["dc.title","Ferritin L and ferritin H are differentially located within hepatic and extra hepatic organs under physiological and acute phase conditions"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]