Tarantola, Marco

[["dc.bibliographiccitation.artnumber","e54172"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLoS One"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Schaefer, Edith"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Polo, Elena"],["dc.contributor.author","Westendorf, Christian"],["dc.contributor.author","Oikawa, Noriko"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T09:29:07Z"],["dc.date.available","2018-11-07T09:29:07Z"],["dc.date.issued","2013"],["dc.description.abstract","Chemotactic responses of Dictyostelium discoideum cells to periodic self-generated signals of extracellular cAMP comprise a large number of intricate morphological changes on different length scales. Here, we scrutinized chemotaxis of single Dictyostelium discoideum cells under conditions of starvation using a variety of optical, electrical and acoustic methods. Amebas were seeded on gold electrodes displaying impedance oscillations that were simultaneously analyzed by optical video microscopy to relate synchronous changes in cell density, morphology, and distance from the surface to the transient impedance signal. We found that starved amebas periodically reduce their overall distance from the surface producing a larger impedance and higher total fluorescence intensity in total internal reflection fluorescence microscopy. Therefore, we propose that the dominant sources of the observed impedance oscillations observed on electric cell-substrate impedance sensing electrodes are periodic changes of the overall cell-substrate distance of a cell. These synchronous changes of the cell-electrode distance were also observed in the oscillating signal of acoustic resonators covered with amebas. We also found that periodic cell-cell aggregation into transient clusters correlates with changes in the cell-substrate distance and might also contribute to the impedance signal. It turned out that cell-cell contacts as well as cell-substrate contacts form synchronously during chemotaxis of Dictyostelium discoideum cells."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.description.sponsorship","DFG [SFB 937]"],["dc.identifier.doi","10.1371/journal.pone.0054172"],["dc.identifier.fs","599447"],["dc.identifier.isi","000313738900049"],["dc.identifier.pmid","23349816"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8517"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30944"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights.access","openAccess"],["dc.title","Chemotaxis of Dictyostelium discoideum: Collective Oscillation of Cellular Contacts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e23894"],["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Communicative & Integrative Biology"],["dc.bibliographiccitation.lastpage","4"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Schäfer, Edith"],["dc.contributor.author","Aue, Dennis"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Polo, Elena"],["dc.contributor.author","Westendorf, Christian"],["dc.contributor.author","Oikawa, Noriko"],["dc.contributor.author","Bodenschatz, Eberhard"],["dc.contributor.author","Geil, Burkhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2019-07-09T11:54:16Z"],["dc.date.available","2019-07-09T11:54:16Z"],["dc.date.issued","2013"],["dc.description.abstract","Dictyostelium discoideum cells respond to periodic signals of extracellular cAMP by collective changes of cell-cell and cell-substrate contacts. This was confirmed by dielectric analysis employing electric cell-substrate impedance sensing (ECIS) and impedance measurements involving cell-filled micro channels in conjunction with optical microscopy providing a comprehensive picture of chemotaxis under conditions of starvation."],["dc.identifier.doi","10.4161/cib.23894"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8716"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/60608"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1942-0889"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Collective behavior of Dictyostelium discoideum monitored by impedance analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12070"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Dietz, Jörn"],["dc.contributor.author","Turco, Laura"],["dc.contributor.author","Halder, Partho"],["dc.contributor.author","Polo, Elena"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2019-07-09T11:44:35Z"],["dc.date.available","2019-07-09T11:44:35Z"],["dc.date.issued","2017-09-21"],["dc.description.abstract","The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25. Two different strategies were realized; one based on supported bilayers and the other one employing sessile giant liposomes. In the first approach, isolated patches of planar bilayers derived from giant unilamellar vesicles containing syntaxin 1 and preassembled SNAP 25 (ΔN-complex) were deposited on a dilatable PDMS sheet. In a second approach, lateral membrane tension was controlled through the adhesion of intact giant unilamellar vesicles on a functionalized surface. In both approaches fusion efficiency increases considerably with lateral tension and we identified a threshold tension of 3.4 mN m(-1), at which the number of fusion events is increased substantially."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1038/s41598-017-12348-w"],["dc.identifier.pmid","28935937"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14824"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59040"],["dc.language.iso","en"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.subject.ddc","540"],["dc.title","Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]