Tarantola, Marco

[["dc.bibliographiccitation.firstpage","254"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Nanotoxicology"],["dc.bibliographiccitation.lastpage","268"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Rother, Jan"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Pierrat, Sebastien"],["dc.contributor.author","Soennichsen, Carsten"],["dc.contributor.author","Wegener, Joachim"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:55:39Z"],["dc.date.available","2018-11-07T08:55:39Z"],["dc.date.issued","2011"],["dc.description.abstract","Nanoparticle exposure is monitored by a combination of two label-free and non-invasive biosensor devices which detect cellular shape and viscoelasticity (quartz crystal microbalance), cell motility and the dynamics of epithelial cell-cell contacts (electric cell-substrate impedance sensing). With these tools we have studied the impact of nanoparticle shape on cellular physiology. Gold (Au) nanoparticles coated with CTAB were synthesized and studied in two distinct shapes: Spheres with a diameter of (43 +/-+/- 4) nm and rods with a size of (38 +/-+/- 7) nm xx (17 +/-+/- 3) nm. Dose-response experiments were accompanied by conventional cytotoxicity tests as well as fluorescence and dark-field microscopy to visualize the intracellular particle distribution. We found that spherical gold nanoparticles with identical surface functionalization are generally more toxic and more efficiently ingested than rod-shaped particles. We largely attribute the higher toxicity of CTAB-coated spheres as compared to rod-shaped particles to a higher release of toxic CTAB upon intracellular aggregation."],["dc.description.sponsorship","German Science Foundation (DFG) [JA 963/10-1]"],["dc.identifier.doi","10.3109/17435390.2010.528847"],["dc.identifier.isi","000290936000013"],["dc.identifier.pmid","21050076"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22954"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.relation.issn","1743-5404"],["dc.relation.issn","1743-5390"],["dc.title","Toxicity of gold-nanoparticles: Synergistic effects of shape and surface functionalization on micromotility of epithelial cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","223"],["dc.bibliographiccitation.journal","Beilstein Journal of Nanotechnology"],["dc.bibliographiccitation.lastpage","231"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Pietuch, Anna"],["dc.contributor.author","Brueckner, Bastian Rouven"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Soennichsen, Carsten"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T10:02:05Z"],["dc.date.available","2018-11-07T10:02:05Z"],["dc.date.issued","2015"],["dc.description.abstract","Background: The impact of gold nanoparticles on cell viability has been extensively studied in the past. Size, shape and surface functionalization including opsonization of gold particles ranging from a few nanometers to hundreds of nanometers are among the most crucial parameters that have been focussed on. Cytoxicity of nanomaterial has been assessed by common cytotoxicity assays targeting enzymatic activity such as LDH, MTT and ECIS. So far, however, less attention has been paid to the mechanical parameters of cells exposed to gold particles, which is an important reporter on the cellular response to external stimuli. Results: Mechanical properties of confluent MDCK II cells exposed to gold nanorods as a function of surface functionalization and concentration have been explored by atomic force microscopy and quartz crystal microbalance measurements in combination with fluorescence and dark-field microscopy. Conclusion: We found that cells exposed to CTAB coated gold nanorods display a concentration-dependent stiffening that cannot be explained by the presence of CTAB alone. The stiffening results presumably from endocytosis of particles removing excess membrane area from the cell's surface. Another aspect could be the collapse of the plasma membrane on the actin cortex. Particles coated with PEG do not show a significant change in elastic properties. This observation is consistent with QCM measurements that show a considerable drop in frequency upon administration of CTAB coated rods suggesting an increase in acoustic load corresponding to a larger stiffness (storage modulus)."],["dc.description.sponsorship","DFG"],["dc.identifier.doi","10.3762/bjnano.6.21"],["dc.identifier.isi","000348943900001"],["dc.identifier.pmid","25671166"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38155"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Beilstein-institut"],["dc.relation.issn","2190-4286"],["dc.title","Mechanical properties of MDCK II cells exposed to gold nanorods"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3683"],["dc.bibliographiccitation.issue","23"],["dc.bibliographiccitation.journal","Small"],["dc.bibliographiccitation.lastpage","3690"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Pierrat, Sebastien"],["dc.contributor.author","Henkel, Andreas"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Soennichsen, Carsten"],["dc.date.accessioned","2018-11-07T09:02:22Z"],["dc.date.available","2018-11-07T09:02:22Z"],["dc.date.issued","2012"],["dc.description.abstract","Toxicological effects of nanoparticles are associated with their internalization into cells. Hence, there is a strong need for techniques revealing the interaction between particles and cells as well as quantifying the uptake at the same time. For that reason, herein optical dark-field microscopy is used in conjunction with transmission electron microscopy to investigate the uptake of gold nanoparticles into epithelial cells with respect to shape, stabilizing agent, and surface charge. The number of internalized particles is strongly dependent on the stabilizing agent, but not on the particle shape. A test of metabolic activity shows no direct correlation with the number of internalized particles. Therefore, particle properties besides coating and shape are suspected to contribute to the observed toxicity."],["dc.description.sponsorship","DFG [SPP 1313]"],["dc.identifier.doi","10.1002/smll.201200853"],["dc.identifier.isi","000312214400019"],["dc.identifier.pmid","22888068"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24670"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.issn","1613-6810"],["dc.title","A New Approach to Assess Gold Nanoparticle Uptake by Mammalian Cells: Combining Optical Dark-Field and Transmission Electron Microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2099"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],["dc.bibliographiccitation.lastpage","2107"],["dc.bibliographiccitation.volume","1813"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:49:19Z"],["dc.date.available","2018-11-07T08:49:19Z"],["dc.date.issued","2011"],["dc.description.abstract","The epithelial-to-mesenchymal transition (EMT) is a program of cellular development associated with loss of cell-cell contacts, a decreased cell adhesion and substantial morphological changes. Besides its importance for numerous developmental processes, EMT has also been held responsible for the development and progression of tumors and formation of metastases. The influence of the cytokine transforming growth factor beta 1 (TGF-beta 1) induced EMT on structure, migration, cytoskeletal dynamics and long-term correlations of the mammalian epithelial cell lines NMuMG, A549 and MDA-MB231 was investigated with time-resolved impedance analysis. The three cell lines show important differences in concentration dependency, cellular morphology and dynamics upon their response to TGF-beta 1. A549 cells and the non-tumor mouse epithelial cell line NMuMG show a substantial change in morphology mirrored in stepwise changes of their phenotype upon cytokine treatment. Impedance based measurements of micromotility reveal a complex dynamic response to TGF-beta 1 exposure which leads to a transient increase in fluctuation amplitude and long-term correlation. These changes in fluctuation amplitude are also detectable for MDA-MB231 cells, whereas the long-term correlation remains unvaried. We were able to distinguish three time domains during EMT. Initially, all cell lines display an increase in micromotion lasting 4 to 9 h termed transitional state I. This regime is followed by transitional state II lasting approximately 20 h, where cellular dynamics are diminished and, in case of the NMuMG cell line, a loss of cell-cell contacts occurs. Finally, the transformation into the mesenchymal-like phenotype occurs 24-30 h after exposure to TGF-beta 1. (C) 2011 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","[SFB 937]"],["dc.identifier.doi","10.1016/j.bbamcr.2011.07.016"],["dc.identifier.isi","000297882400013"],["dc.identifier.pmid","21839117"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21433"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0167-4889"],["dc.title","Dynamics of TGF-beta induced epithelial-to-mesenchymal transition monitored by Electric Cell-Substrate Impedance Sensing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","European Biophysics Journal"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:53:36Z"],["dc.date.available","2018-11-07T08:53:36Z"],["dc.date.issued","2011"],["dc.format.extent","163"],["dc.identifier.isi","000293637300426"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22458"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","New york"],["dc.relation.eventlocation","Budapest, HUNGARY"],["dc.relation.issn","0175-7571"],["dc.title","Morphological and dynamical changes during TGF-beta induced epithelial-to-mesenchymal transition"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1494"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Chemical Research in Toxicology"],["dc.bibliographiccitation.lastpage","1506"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Marel, Anna-Kristina"],["dc.contributor.author","Kunze, Angelika"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:52:26Z"],["dc.date.available","2018-11-07T08:52:26Z"],["dc.date.issued","2011"],["dc.description.abstract","Cellular motility is the major driving force of numerous biological phenomena including wound healing, immune response, embryogenesis, cancer formation, and metastasis. We studied the response of epithelial FaDu monolayers cultured on gold electrodes of an acoustic resonator (quartz crystal microbalance, QCM) and impedance sensor (electric cell-substrate impedance sensing, ECIS) to externally applied chemical stimuli interfering with cytoskeleton organization. Epithelial cell motility of confluent monolayers is characterized by subtle cell shape changes and variations in the cell-substrate as well as cell-cell distance without net directionality of individual cells. The impact of small molecules such as cytochalasin D, phalloidin, and blebbistatin as well as paclitaxel, nocodazol, and colchicin on actin and microtubules organization was quantified by conventional sensors' readouts and by comparing the noise pattern of the signals which is attributed to cellular dynamics. The responsiveness of noninvasive and label-free techniques relying on cellular dynamics is compared to classical viability assays and changes of the overall impedance of ultrasmall electrodes or acoustic loads of a thickness shear mode resonator. Depending on the agent used, a distinct sensor response was found, which can be used as a fingerprint of the cellular response. Cytoskeletal rearrangements and nuclear integrity were corroborated by fluorescence microscopy and correlated to the readouts of QCM and ECIS."],["dc.description.sponsorship","graduate school of excellence MAINZ; DFG [SPP 1313, SFB 937]"],["dc.identifier.doi","10.1021/tx200115q"],["dc.identifier.isi","000295058900009"],["dc.identifier.pmid","21815656"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22162"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Chemical Soc"],["dc.relation.issn","1520-5010"],["dc.relation.issn","0893-228X"],["dc.title","Dynamic Changes of Acoustic Load and Complex Impedance as Reporters for the Cytotoxicity of Small Molecule Inhibitors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2479"],["dc.bibliographiccitation.journal","Beilstein Journal of Nanotechnology"],["dc.bibliographiccitation.lastpage","2488"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Pierrat, Sebastien"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Soennichsen, Carsten"],["dc.date.accessioned","2018-11-07T09:31:08Z"],["dc.date.available","2018-11-07T09:31:08Z"],["dc.date.issued","2014"],["dc.description.abstract","In this work, we study epithelial cell growth on substrates decorated with gold nanorods that are functionalized either with a positively charged cytotoxic surfactant or with a biocompatible polymer exhibiting one of two different end groups, resulting in a neutral or negative surface charge of the particle. Upon observation of cell growth for three days by live cell imaging using optical dark field microscopy, it was found that all particles supported cell adhesion while no directed cell migration and no significant particle internalization occurred. Concerning cell adhesion and spreading as compared to cell growth on bare substrates after 3 days of incubation, a reduction by 45% and 95%, respectively, for the surfactant particle coating was observed, whereas the amino-terminated polymer induced a reduction by 30% and 40%, respectively, which is absent for the carboxy-terminated polymer. Furthermore, interface-sensitive impedance spectroscopy (electric cell-substrate impedance sensing, ECIS) was employed in order to investigate the micromotility of cells added to substrates decorated with various amounts of surfactant-coated particles. A surface density of 65 particles/mu m(2) (which corresponds to 0.5% of surface coverage with nanoparticles) diminishes micromotion by 25% as compared to bare substrates after 35 hours of incubation. We conclude that the surface coating of the gold nanorods, which were applied to the basolateral side of the cells, has a recognizable influence on the growth behavior and thus the coating should be carefully selected for biomedical applications of nanoparticles."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SPP1313]"],["dc.identifier.doi","10.3762/bjnano.5.257"],["dc.identifier.fs","613591"],["dc.identifier.isi","000348934300001"],["dc.identifier.pmid","25671143"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12180"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31470"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Beilstein-institut"],["dc.relation.issn","2190-4286"],["dc.rights.access","openAccess"],["dc.title","Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]