Tarantola, Marco

[["dc.bibliographiccitation.firstpage","139"],["dc.bibliographiccitation.issue","2-3"],["dc.bibliographiccitation.journal","Integrative Biology"],["dc.bibliographiccitation.lastpage","150"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Marel, Anna-Kristina"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Adam, Holger"],["dc.contributor.author","Wegener, Joachim"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2018-11-07T08:47:48Z"],["dc.date.available","2018-11-07T08:47:48Z"],["dc.date.issued","2010"],["dc.description.abstract","Early determination of the metastatic potential of cancer cells is a crucial step for successful oncological treatment. Besides the remarkable progress in molecular genomics- or proteomics-based diagnostics, there is a great demand for in vitro biosensor devices that allow rapid and selective detection of the invasive properties of tumor cells. Here, the classical cancer cell motility in vitro assays for migration and invasion relying on Boyden chambers are compared to a real-time biosensor that analyzes the dynamic properties of adherent cells electro-acoustically with a time resolution on the order of seconds. The sensor relies on the well-established quartz crystal microbalance technique (QCM) that measures the shift in resonance frequency and damping of an oscillating quartz crystal when adsorption, desorption or changes in material properties close to the quartz surface occur. In addition, the QCM is capable of detecting the rather subtle fluctuations of the cell bodies as an indicator for their micromotility. QCM-based micromotility readings of three different cancer cell lines (HT-29, HSC-4, FaDu) are compared with the well-known electrical cell-substrate impedance sensing (ECIS) revealing collective stochastic motion that corresponds to the malignancy of the cells."],["dc.description.sponsorship","MAINZ"],["dc.identifier.doi","10.1039/b920815a"],["dc.identifier.isi","000275225800007"],["dc.identifier.pmid","20473392"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7211"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21053"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Royal Soc Chemistry"],["dc.relation.issn","1757-9694"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Dynamics of human cancer cell lines monitored by electrical and acoustic fluctuation analysis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2479"],["dc.bibliographiccitation.journal","Beilstein Journal of Nanotechnology"],["dc.bibliographiccitation.lastpage","2488"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Rosman, Christina"],["dc.contributor.author","Pierrat, Sebastien"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Schneider, David"],["dc.contributor.author","Sunnick, Eva"],["dc.contributor.author","Janshoff, Andreas"],["dc.contributor.author","Soennichsen, Carsten"],["dc.date.accessioned","2018-11-07T09:31:08Z"],["dc.date.available","2018-11-07T09:31:08Z"],["dc.date.issued","2014"],["dc.description.abstract","In this work, we study epithelial cell growth on substrates decorated with gold nanorods that are functionalized either with a positively charged cytotoxic surfactant or with a biocompatible polymer exhibiting one of two different end groups, resulting in a neutral or negative surface charge of the particle. Upon observation of cell growth for three days by live cell imaging using optical dark field microscopy, it was found that all particles supported cell adhesion while no directed cell migration and no significant particle internalization occurred. Concerning cell adhesion and spreading as compared to cell growth on bare substrates after 3 days of incubation, a reduction by 45% and 95%, respectively, for the surfactant particle coating was observed, whereas the amino-terminated polymer induced a reduction by 30% and 40%, respectively, which is absent for the carboxy-terminated polymer. Furthermore, interface-sensitive impedance spectroscopy (electric cell-substrate impedance sensing, ECIS) was employed in order to investigate the micromotility of cells added to substrates decorated with various amounts of surfactant-coated particles. A surface density of 65 particles/mu m(2) (which corresponds to 0.5% of surface coverage with nanoparticles) diminishes micromotion by 25% as compared to bare substrates after 35 hours of incubation. We conclude that the surface coating of the gold nanorods, which were applied to the basolateral side of the cells, has a recognizable influence on the growth behavior and thus the coating should be carefully selected for biomedical applications of nanoparticles."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SPP1313]"],["dc.identifier.doi","10.3762/bjnano.5.257"],["dc.identifier.fs","613591"],["dc.identifier.isi","000348934300001"],["dc.identifier.pmid","25671143"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12180"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31470"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Beilstein-institut"],["dc.relation.issn","2190-4286"],["dc.rights.access","openAccess"],["dc.title","Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]