Tarantola, Marco

[["dc.bibliographiccitation.artnumber","12070"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific reports"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Kliesch, Torben-Tobias"],["dc.contributor.author","Dietz, Jörn"],["dc.contributor.author","Turco, Laura"],["dc.contributor.author","Halder, Partho"],["dc.contributor.author","Polo, Elena"],["dc.contributor.author","Tarantola, Marco"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Janshoff, Andreas"],["dc.date.accessioned","2019-07-09T11:44:35Z"],["dc.date.available","2019-07-09T11:44:35Z"],["dc.date.issued","2017-09-21"],["dc.description.abstract","The large gap in time scales between membrane fusion occurring in biological systems during neurotransmitter release and fusion observed between model membranes has provoked speculations over a large number of possible factors that might explain this discrepancy. One possible reason is an elevated lateral membrane tension present in the presynaptic membrane. We investigated the tension-dependency of fusion using model membranes equipped with a minimal fusion machinery consisting of syntaxin 1, synaptobrevin and SNAP 25. Two different strategies were realized; one based on supported bilayers and the other one employing sessile giant liposomes. In the first approach, isolated patches of planar bilayers derived from giant unilamellar vesicles containing syntaxin 1 and preassembled SNAP 25 (ΔN-complex) were deposited on a dilatable PDMS sheet. In a second approach, lateral membrane tension was controlled through the adhesion of intact giant unilamellar vesicles on a functionalized surface. In both approaches fusion efficiency increases considerably with lateral tension and we identified a threshold tension of 3.4 mN m(-1), at which the number of fusion events is increased substantially."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2017"],["dc.identifier.doi","10.1038/s41598-017-12348-w"],["dc.identifier.pmid","28935937"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14824"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59040"],["dc.language.iso","en"],["dc.relation.issn","2045-2322"],["dc.rights.access","openAccess"],["dc.subject.ddc","540"],["dc.title","Membrane tension increases fusion efficiency of model membranes in the presence of SNAREs."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]