Keller, Jan

[["dc.bibliographiccitation.firstpage","11440"],["dc.bibliographiccitation.issue","31"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","11445"],["dc.bibliographiccitation.volume","103"],["dc.contributor.author","Donnert, Gerald"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Andrei, M. Alexandra"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Lührmann, Reinhard"],["dc.contributor.author","Jahn, Reinhard"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:52:39Z"],["dc.date.available","2017-09-07T11:52:39Z"],["dc.date.issued","2006"],["dc.description.abstract","We demonstrate far-field fluorescence microscopy with a focal-plane resolution of 15-20 nm in biological samples. The 10- to 12-fold multilateral increase in resolution below the diffraction barrier has been enabled by the elimination of molecular triplet state excitation as a major source of photobleaching of a number of dyes in stimulated emission depletion microscopy. Allowing for relaxation of the triplet state between subsequent excitation-depletion cycles yields an up to 30-fold increase in total fluorescence signal as compared with reported stimulated emission depletion illumination schemes. Moreover, it enables the reduction of the effective focal spot area by up to approximate to 140-fold below that given by diffraction. Triplet-state relaxation can be realized either by reducing the repetition rate of pulsed lasers or by increasing the scanning speed such that the build-up of the triplet state is effectively prevented. This resolution in immunofluorescence imaging is evidenced by revealing nanoscale protein patterns on endosomes, the punctuated structures of intermediate filaments in neurons, and nuclear protein speckles in mammalian cells with conventional optics. The reported performance of diffraction-unlimited fluorescence microscopy opens up a pathway for addressing fundamental problems in the life sciences."],["dc.identifier.doi","10.1073/pnas.0604965103"],["dc.identifier.gro","3143651"],["dc.identifier.isi","000239616400005"],["dc.identifier.pmid","16864773"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1188"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0027-8424"],["dc.title","Macromolecular-scale resolution in biological fluorescence microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1528"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Zeitschrift für Gastroenterologie"],["dc.bibliographiccitation.lastpage","1572"],["dc.bibliographiccitation.volume","60"],["dc.contributor.author","Andresen, V."],["dc.contributor.author","Becker, G."],["dc.contributor.author","Frieling, T."],["dc.contributor.author","Goebel-Stengel, M."],["dc.contributor.author","Gundling, F."],["dc.contributor.author","Herold, A."],["dc.contributor.author","Karaus, M."],["dc.contributor.author","Keller, J."],["dc.contributor.author","Kim, M."],["dc.contributor.author","Klose, P."],["dc.contributor.author","Pehl, C."],["dc.contributor.authorgroup","In Zusammenarbeit mit:"],["dc.contributor.authorgroup","Deutsche Gesellschaft für Allgemein- und Viszeralchirurgie (DGAV)/Chirurgische Arbeitsgemeinschaft für Coloproktologie (CACP)"],["dc.contributor.authorgroup","Deutsche Gesellschaft für Ernährungsmedizin (DGEM), Deutsche Gesellschaft für Naturheilkunde (DGNHK)"],["dc.contributor.authorgroup","Deutsche Gesellschaft für Geriatrie (DGG)"],["dc.contributor.authorgroup","Deutsche Gesellschaft für Koloproktologie (DGK)"],["dc.contributor.authorgroup","Deutsche Gesellschaft für Innere Medizin (DGIM)"],["dc.contributor.authorgroup","Deutsche Gesellschaft für Palliativmedizin e. V. (DGP)"],["dc.contributor.authorgroup","Deutsche Schmerzgesellschaft e. V., MAGDA Patientenforum (MAGDA)"],["dc.date.accessioned","2022-11-01T10:16:50Z"],["dc.date.available","2022-11-01T10:16:50Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1055/a-1880-1928"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/116665"],["dc.language.iso","de"],["dc.notes.intern","DOI-Import GROB-605"],["dc.relation.eissn","1439-7803"],["dc.relation.issn","0044-2771"],["dc.title","Aktualisierte S2k-Leitlinie chronische Obstipation der Deutschen Gesellschaft für Gastroenterologie, Verdauungs- und Stoffwechselkrankheiten (DGVS) und der Deutschen Gesellschaft für Neurogastroenterologie & Motilität (DGNM) – April 2022 – AWMF-Registriernummer: 021–019"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3361"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","3371"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:49:49Z"],["dc.date.available","2017-09-07T11:49:49Z"],["dc.date.issued","2007"],["dc.description.abstract","By exploiting the saturation of a reversible single photon transition, RESOLFT microscopy is capable of resolving three dimensional structures inside specimen with a resolution that is no longer limited by the wavelength of the light in use. The transition is driven by a spatially varying intensity distribution that features at least one isolated point, line or plane with zero intensity and the resolution achieved depends critically on the field distribution around these zeros. Based on a vectorial analysis of the image formation in a RESOLFT microscope, we develop a method to effectively search for optimal zero intensity point patterns under typical experimental conditions. Using this approach, we derived a spatial intensity distribution that optimizes the focal plane resolution. Moreover, we outline a general strategy that allows optimization of the resolution for a given experimental situation and present solutions for the most common cases in biological imaging."],["dc.identifier.doi","10.1364/OE.15.003361"],["dc.identifier.gro","3143520"],["dc.identifier.isi","000245076200065"],["dc.identifier.pmid","19532577"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1043"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","Efficient fluorescence inhibition patterns for RESOLFT microscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","675"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","684"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Rizzoli, Silvio"],["dc.date.accessioned","2017-09-07T11:45:20Z"],["dc.date.available","2017-09-07T11:45:20Z"],["dc.date.issued","2010"],["dc.description.abstract","Synaptic vesicles need to be mobile to reach their release sites during synaptic activity. We investigated vesicle mobility throughout the synaptic vesicle cycle using both conventional and subdiffraction-resolution stimulated emission depletion fluorescence microscopy. Vesicle tracking revealed that recently endocytosed synaptic vesicles are highly mobile for a substantial time period after endocytosis. They later undergo a maturation process and integrate into vesicle clusters where they exhibit little mobility. Despite the differences in mobility, both recently endocytosed and mature vesicles are exchanged between synapses. Electrical stimulation does not seem to affect the mobility of the two types of vesicles. After exocytosis, the vesicle material is mobile in the plasma membrane, although the movement appears to be somewhat limited. Increasing the proportion of fused vesicles (by stimulating exocytosis while simultaneously blocking endocytosis) leads to substantially higher mobility. We conclude that both high- and low-mobility states are characteristic of synaptic vesicle movement."],["dc.identifier.doi","10.1016/j.bpj.2010.04.054"],["dc.identifier.gro","3142885"],["dc.identifier.isi","000280182300042"],["dc.identifier.pmid","20643088"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/338"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0006-3495"],["dc.title","High- and Low-Mobility Stages in the Synaptic Vesicle Cycle"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","417"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Journal of Biophotonics"],["dc.bibliographiccitation.lastpage","424"],["dc.bibliographiccitation.volume","3"],["dc.bibliographiccitation.volumetitle","Advanced Micro and Nanoscopy for Biomedicine"],["dc.contributor.author","Lauterbach, Marcel A."],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Schönle, Andreas"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Rizzoli, Silvio"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:45:57Z"],["dc.date.available","2017-09-07T11:45:57Z"],["dc.date.issued","2010"],["dc.description.abstract","We compare the performance of video-rate Stimulated Emission Depletion (STED) and confocal microscopy in imaging the interior of living neurons. A lateral resolution of 65 nm is observed in STED movies of 28 frames per second, which is 4-fold higher in spatial resolution than in their confocal counterparts. S FED microscopy, but not confocal microscopy, allows discrimination of single features at high spatial densities. Specific patterns of movement within the confined space of the axon are revealed in STED microscopy, while confocal imaging is limited to reporting gross motion. Further progress is to be expected, as we demonstrate that the use of continuous wave (CW) beams for excitation and STED is viable for video-rate STED recording of living neurons. Tentatively providing a larger photon flux, CW beams should facilitate extending fast STED imaging towards imaging fainter living samples. [GRAPHICS] Synaptic vesicles within an axon. Single frames of movies recorded at 28 frames per second. Confocal microscopy (left) can only reproduce the axon, whereas STED microscopy (right) can resolve moving objects in the neuron; '+' indicates that the data are linearly deconvolved."],["dc.identifier.doi","10.1002/jbio.201000038"],["dc.identifier.gro","3142897"],["dc.identifier.isi","000279969600003"],["dc.identifier.pmid","20379984"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/352"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1864-063X"],["dc.title","Comparing video-rate STED nanoscopy and confocal microscopy of living neurons"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","11454"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Andrade, Débora Machado"],["dc.contributor.author","Clausen, Mathias P."],["dc.contributor.author","Keller, Jan"],["dc.contributor.author","Mueller, Veronika"],["dc.contributor.author","Wu, Congying"],["dc.contributor.author","Bear, James E."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Lagerholm, B. Christoffer"],["dc.contributor.author","Eggeling, Christian"],["dc.date.accessioned","2017-09-07T11:43:45Z"],["dc.date.available","2017-09-07T11:43:45Z"],["dc.date.issued","2015"],["dc.description.abstract","Important discoveries in the last decades have changed our view of the plasma membrane organisation. Specifically, the cortical cytoskeleton has emerged as a key modulator of the lateral diffusion of membrane proteins. Cytoskeleton-dependent compartmentalised lipid diffusion has been proposed, but this concept remains controversial because this phenomenon has thus far only been observed with artefact-prone probes in combination with a single technique: single particle tracking. In this paper, we report the first direct observation of compartmentalised phospholipid diffusion in the plasma membrane of living cells using a minimally invasive, fluorescent dye labelled lipid analogue. These observations were made using optical STED nanoscopy in combination with fluorescence correlation spectroscopy (STED-FCS), a technique which allows the study of membrane dynamics on a sub-millisecond time-scale and with a spatial resolution of down to 40 nm. Specifically, we find that compartmentalised phospholipid diffusion depends on the cortical actin cytoskeleton, and that this constrained diffusion is directly dependent on the F-actin branching nucleator Arp2/3. These findings provide solid evidence that the Arp2/3-dependent cortical actin cytoskeleton plays a pivotal role in the dynamic organisation of the plasma membrane, potentially regulating fundamental cellular processes."],["dc.identifier.doi","10.1038/srep11454"],["dc.identifier.gro","3141881"],["dc.identifier.isi","000357041500001"],["dc.identifier.pmid","26118385"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2111"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2045-2322"],["dc.title","Cortical actin networks induce spatio-temporal confinement of phospholipids in the plasma membrane - a minimally invasive investigation by STED-FCS"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]