Fuchs, Eberhard

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","190"],["dc.bibliographiccitation.journal","BMC genomics"],["dc.bibliographiccitation.lastpage","9"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Datson, Nicole A."],["dc.contributor.author","Morsink, Maarten C."],["dc.contributor.author","Atanasova, Srebrena"],["dc.contributor.author","Armstrong, Victor W."],["dc.contributor.author","Zischler, Hans"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Dutilh, Bas E."],["dc.contributor.author","Huynen, Martijn A."],["dc.contributor.author","Waegele, Brigitte"],["dc.contributor.author","Ruepp, Andreas"],["dc.contributor.author","Kloet, E. Ronald"],["dc.contributor.author","Fuchs, Eberhard"],["dc.date.accessioned","2019-07-10T08:13:00Z"],["dc.date.available","2019-07-10T08:13:00Z"],["dc.date.issued","2007"],["dc.description.abstract","Background: The common marmoset monkey (Callithrix jacchus), a small non-endangered New World primate native to eastern Brazil, is becoming increasingly used as a non-human primate model in biomedical research, drug development and safety assessment. In contrast to the growing interest for the marmoset as an animal model, the molecular tools for genetic analysis are extremely limited.Results: Here we report the development of the first marmoset-specific oligonucleotide microarray (EUMAMA) containing probe sets targeting 1541 different marmoset transcripts expressed in hippocampus. These 1541 transcripts represent a wide variety of different functional gene classes. Hybridisation of the marmoset microarray with labelled RNA from hippocampus, cortex and a panel of 7 different peripheral tissues resulted in high detection rates of 85% in the neuronal tissues and on average 70% in the non-neuronal tissues. The expression profiles of the 2 neuronal tissues, hippocampus and cortex, were highly similar, as indicated by a correlation coefficient of 0.96. Several transcripts with a tissue-specific pattern of expression were identified. Besides the marmoset microarray we have generated 3215 ESTs derived from marmoset hippocampus, which have been annotated and submitted to GenBank [GenBank: EF214838 EF215447, EH380242 EH382846]. Conclusion: We have generated the first marmoset-specific DNA microarray and demonstrated its use to characterise large-scale gene expression profiles of hippocampus but also of other neuronal and non-neuronal tissues. In addition, we have generated a large collection of ESTs of marmoset origin, which are now available in the public domain. These new tools will facilitate molecular genetic research into this non-human primate animal model."],["dc.identifier.fs","91281"],["dc.identifier.ppn","560256167"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/4367"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/61097"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.orgunit","Universitätsmedizin Göttingen"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.subject.ddc","610"],["dc.title","Development of the first marmoset-specific DNA microarray (EUMAMA): a new genetic tool for large-scale expression profiling in a non-human primate"],["dc.title.alternative","Research article"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","PII 905441944"],["dc.bibliographiccitation.firstpage","215"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Stress"],["dc.bibliographiccitation.lastpage","224"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Atanasova, Srebrena"],["dc.contributor.author","Wieland, Eberhard"],["dc.contributor.author","Schlumbohm, Christina"],["dc.contributor.author","Korecka, M."],["dc.contributor.author","Shaw, Leslie M."],["dc.contributor.author","von Ahsen, Nicolas"],["dc.contributor.author","Fuchs, E."],["dc.contributor.author","Oellerich, M."],["dc.contributor.author","Armstrong, Victor William"],["dc.date.accessioned","2018-11-07T08:35:33Z"],["dc.date.available","2018-11-07T08:35:33Z"],["dc.date.issued","2009"],["dc.description.abstract","Human epidemiological studies have indicated that low birth weight associated with an adverse intrauterine environment is related to a greater incidence of cardiovascular disorders in later life. In the foetus, endogenous glucocorticoids generally increase if there is intrauterine nutrient deficiency. The consequent glucocorticoid hyperexposure has been hypothesised to cause in utero programming of atherogenic genes. We investigated the effect of oral treatment with the synthetic glucocorticoid dexamethasone during early or late pregnancy in marmoset monkeys on oxidative and antioxidant status in the offspring. Urinary concentrations of F2-isoprostanes were quantified as markers for in vivo oxidative stress. Expression of the mRNAs for the antioxidant enzymes cytosolic glutathione peroxidase (GPx-1), phospholipid hydroperoxide glutathione peroxidase (GPx-4), cytosolic Cu,Zn-superoxide dismutase (SOD1), mitochondrial Mn-superoxide dismutase (SOD2), glutathione reductase (GSR), modifier subunit of glutamate-cysteine ligase (GCLM) and catalase were determined in the aorta. Three groups of pregnant marmosets (10 animals per group) were treated orally for one week with vehicle, or with dexamethasone (5mg/kg daily) during two gestation windows: early dexamethasone group, pregnancy day 42-48, and late dexamethasone group, pregnancy day 90-96. In one male sibling of each litter (10 males per group), aortas were taken at 2 years of age. In the late dexamethasone group a higher aortic mRNA expression for GPx-1 (p0.023), MnSOD (p0.016), GCLM (p0.019) and GSR (p0.014) in comparison to the controls was observed. Aortic expression in the early dexamethasone group was statistically significantly higher only for GSR mRNA (p0.038). No significant changes in urinary F2-isoprostane concentrations between controls, early and late dexamethasone groups at 2 years of age were observed. Hence, prenatal exposure to dexamethasone in the third trimester leads to increased mRNA expression of several aortic antioxidant enzymes in the offspring. This expression pattern was not temporally related to oxidative stress, and it may reflect in utero re-programming of aortic antioxidant gene expression during prenatal glucocorticoid exposure."],["dc.description.sponsorship","European Commission [QLRI-CT-2002-02758, EUPEAH]"],["dc.identifier.doi","10.1080/10253890802305075"],["dc.identifier.isi","000265583600003"],["dc.identifier.pmid","19005875"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18097"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.relation.issn","1025-3890"],["dc.title","Prenatal dexamethasone exposure in the common marmoset monkey enhances gene expression of antioxidant enzymes in the aorta of adult offspring"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]