Heimel, Kai

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Genetics"],["dc.bibliographiccitation.volume","220"],["dc.contributor.author","de la Torre, Antonio"],["dc.contributor.author","Jurca, Matteo"],["dc.contributor.author","Hoffmann, Kai"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Kämper, Jörg"],["dc.contributor.author","Pérez-Martín, José"],["dc.contributor.editor","Glass, N L"],["dc.date.accessioned","2022-02-01T10:31:21Z"],["dc.date.available","2022-02-01T10:31:21Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.identifier.doi","10.1093/genetics/iyab152"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98838"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1943-2631"],["dc.title","Robust Cre recombinase activity in the biotrophic smut fungus Ustilago maydis enables efficient conditional null mutants in planta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2388"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","The Plant Cell"],["dc.bibliographiccitation.lastpage","2401"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Scherer, Mario"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Starke, Verena"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2022-04-05T15:35:33Z"],["dc.date.available","2022-04-05T15:35:33Z"],["dc.date.issued","2006-09"],["dc.description.abstract","In the phytopathogenic fungus Ustilago maydis, pathogenic development is controlled by a heterodimer of the two homeodomain proteins bE and bW, encoded by the b-mating-type locus. We have identified a b-dependently induced gene, clampless1 (clp1), that is required for the proliferation of dikaryotic filaments in planta. We show that U. maydis hyphae develop structures functionally equivalent to clamp cells that participate in the distribution of nuclei during cell division. In clp1 mutant strains, dikaryotic filaments penetrate the plant cuticle, but development is stalled before the first mitotic division, and the clamp-like structures are not formed. Although clp1 is immediately activated upon b-induction on the transcriptional level, nuclear-localized Clp1 protein is first observed at the stage of plant penetration prior to the first cell division. Induced expression of clp1 strongly interferes with b-dependent gene regulation and blocks b-dependent filament formation and b-dependent cell cycle arrest. We speculate that the Clp1 protein inhibits the activity of the bE/bW heterodimer to facilitate the cell cycle progression during hyphal growth."],["dc.identifier.doi","10.1105/tpc.106.043521"],["dc.identifier.pmid","16920779"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106404"],["dc.language.iso","en"],["dc.relation.issn","1040-4651"],["dc.title","The Clp1 protein is required for clamp formation and pathogenic development of Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1001035"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS Pathogens"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Scherer, Mario"],["dc.contributor.author","Vranes, Miroslav"],["dc.contributor.author","Wahl, Ramon"],["dc.contributor.author","Pothiratana, Chetsada"],["dc.contributor.author","Schuler, David"],["dc.contributor.author","Vincon, Volker"],["dc.contributor.author","Finkernagel, Florian"],["dc.contributor.author","Flor-Parra, Ignacio"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2022-04-05T15:35:54Z"],["dc.date.available","2022-04-05T15:35:54Z"],["dc.date.issued","2010-08-05"],["dc.description.abstract","In the phytopathogenic basidiomycete Ustilago maydis, sexual and pathogenic development are tightly connected and controlled by the heterodimeric bE/bW transcription factor complex encoded by the b-mating type locus. The formation of the active bE/bW heterodimer leads to the formation of filaments, induces a G2 cell cycle arrest, and triggers pathogenicity. Here, we identify a set of 345 bE/bW responsive genes which show altered expression during these developmental changes; several of these genes are associated with cell cycle coordination, morphogenesis and pathogenicity. 90% of the genes that show altered expression upon bE/bW-activation require the zinc finger transcription factor Rbf1, one of the few factors directly regulated by the bE/bW heterodimer. Rbf1 is a novel master regulator in a multilayered network of transcription factors that facilitates the complex regulatory traits of sexual and pathogenic development."],["dc.identifier.doi","10.1371/journal.ppat.1001035"],["dc.identifier.pmid","20700446"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106406"],["dc.language.iso","en"],["dc.relation.eissn","1553-7374"],["dc.title","The transcription factor Rbf1 is the master regulator for b-mating type controlled pathogenic development in Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1118"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Molecular Plant-Microbe Interactions"],["dc.bibliographiccitation.lastpage","1129"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Zahiri, Alexander"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Wahl, Ramon"],["dc.contributor.author","Rath, Magnus"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2019-11-13T08:34:49Z"],["dc.date.available","2019-11-13T08:34:49Z"],["dc.date.issued","2010"],["dc.description.abstract","Ustilago maydis is a plant-pathogenic fungus that establishes a biotrophic relationship with its host plant, Zea mays. The pathogenic stage of U. maydis is initiated by the fusion of two haploid cells, resulting in the formation of a dikaryotic hypha that invades the plant cell. The switch from saprophytic, yeast-like cells to the biotrophic hyphae requires the complex regulation of a multitude of biological processes to constitute the compatible host-fungus interaction. Transcriptional regulators involved in the establishment of the infectious dikaryon and penetration of the host tissue have been identified; however, regulators required during the post-penetration stages remained to be elucidated. In this study, we report the identification of a U. maydis forkhead transcription factor, Fox1, which is exclusively expressed during biotrophic development. Deletion of fox1 results in reduced virulence and impaired tumor development. The Deltafox1 hyphae induce the accumulation of H(2)O(2) in and around infected cells and a maize defense response phenotypically represented by the encasement of proliferating hyphae in a cellulose-containing matrix. The phenotype can be attributed to the fox1-dependent deregulation of several effector genes that are linked to pathogenic development and host defense suppression."],["dc.identifier.doi","10.1094/MPMI-23-9-1118"],["dc.identifier.pmid","20687802"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62614"],["dc.language.iso","en"],["dc.relation.issn","0894-0282"],["dc.title","The Ustilago maydis forkhead transcription factor Fox1 is involved in the regulation of genes required for the attenuation of plant defenses during pathogenic development"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]