Heimel, Kai

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Genetics"],["dc.bibliographiccitation.volume","220"],["dc.contributor.author","de la Torre, Antonio"],["dc.contributor.author","Jurca, Matteo"],["dc.contributor.author","Hoffmann, Kai"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Kämper, Jörg"],["dc.contributor.author","Pérez-Martín, José"],["dc.contributor.editor","Glass, N L"],["dc.date.accessioned","2022-02-01T10:31:21Z"],["dc.date.available","2022-02-01T10:31:21Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.identifier.doi","10.1093/genetics/iyab152"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98838"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1943-2631"],["dc.title","Robust Cre recombinase activity in the biotrophic smut fungus Ustilago maydis enables efficient conditional null mutants in planta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1812"],["dc.bibliographiccitation.journal","F1000Research"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","McCotter, Sean"],["dc.contributor.author","Kretschmer, Matthias"],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Heimel, Kai"],["dc.date.accessioned","2019-11-13T08:31:54Z"],["dc.date.available","2019-11-13T08:31:54Z"],["dc.date.issued","2018"],["dc.description.abstract","Biotrophic fungal pathogens of plants must sense and adapt to the host environment to complete their life cycles. Recent transcriptome studies of the infection of maize by the biotrophic pathogen Ustilago maydis are providing molecular insights into an ordered program of changes in gene expression and the deployment of effectors as well as key features of nutrient acquisition. In particular, the transcriptome data provide a deeper appreciation of the complexity of the transcription factor network that controls the biotrophic program of invasion, proliferation, and sporulation. Additionally, transcriptome analysis during tumor formation, a key late stage in the life cycle, revealed features of the remodeling of host and pathogen metabolism that may support the formation of tremendous numbers of spores. Transcriptome studies are also appearing for other smut species during interactions with their hosts, thereby providing opportunities for comparative approaches to understand biotrophic adaptation."],["dc.identifier.doi","10.12688/f1000research.16404.1"],["dc.identifier.pmid","30519451"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62613"],["dc.language.iso","en"],["dc.relation.eissn","2046-1402"],["dc.relation.issn","2046-1402"],["dc.title","Transcripts and tumors: regulatory and metabolic programming during biotrophic phytopathogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]