Heimel, Kai

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Genetics"],["dc.bibliographiccitation.volume","220"],["dc.contributor.author","de la Torre, Antonio"],["dc.contributor.author","Jurca, Matteo"],["dc.contributor.author","Hoffmann, Kai"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Kämper, Jörg"],["dc.contributor.author","Pérez-Martín, José"],["dc.contributor.editor","Glass, N L"],["dc.date.accessioned","2022-02-01T10:31:21Z"],["dc.date.available","2022-02-01T10:31:21Z"],["dc.date.issued","2021"],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.description.abstract","Abstract Site-specific recombinases have been used in higher eukaryotes, especially in animals, for a broad range of applications, including chromosomal translocations, large deletions, site-specific integration, and tissue-specific as well as conditional knock-outs. The application of site-specific recombination has also been demonstrated in simple eukaryotes like fungi and protozoa. However, its use in fungal research, especially in phytopathogenic fungi, has often been limited to “recycle” the marker genes used in transformation experiments. We show that Cre recombinase can be used for conditional gene deletions in the phytopathogenic fungus Ustilago maydis. Conditional gene knock-outs can be generated via the transcriptional control of the recombinase by U. maydis promoters specifically activated during the biotrophic phase of fungal growth, enabling gene deletions at defined developmental stages inside the plant tissue. Also, we show that a tamoxifen-activated Cre-recombinase allows the tight control necessary for the induced deletion of essential genes by the addition of tamoxifen. These tools will be helpful to address the function of genes under both axenic and in planta conditions for the U. maydis-maize pathosystem and should pave the way for similar approaches in other plant pathosystems."],["dc.identifier.doi","10.1093/genetics/iyab152"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/98838"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-517"],["dc.relation.eissn","1943-2631"],["dc.title","Robust Cre recombinase activity in the biotrophic smut fungus Ustilago maydis enables efficient conditional null mutants in planta"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1812"],["dc.bibliographiccitation.journal","F1000Research"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Schmitz, Lara"],["dc.contributor.author","McCotter, Sean"],["dc.contributor.author","Kretschmer, Matthias"],["dc.contributor.author","Kronstad, James W."],["dc.contributor.author","Heimel, Kai"],["dc.date.accessioned","2019-11-13T08:31:54Z"],["dc.date.available","2019-11-13T08:31:54Z"],["dc.date.issued","2018"],["dc.description.abstract","Biotrophic fungal pathogens of plants must sense and adapt to the host environment to complete their life cycles. Recent transcriptome studies of the infection of maize by the biotrophic pathogen Ustilago maydis are providing molecular insights into an ordered program of changes in gene expression and the deployment of effectors as well as key features of nutrient acquisition. In particular, the transcriptome data provide a deeper appreciation of the complexity of the transcription factor network that controls the biotrophic program of invasion, proliferation, and sporulation. Additionally, transcriptome analysis during tumor formation, a key late stage in the life cycle, revealed features of the remodeling of host and pathogen metabolism that may support the formation of tremendous numbers of spores. Transcriptome studies are also appearing for other smut species during interactions with their hosts, thereby providing opportunities for comparative approaches to understand biotrophic adaptation."],["dc.identifier.doi","10.12688/f1000research.16404.1"],["dc.identifier.pmid","30519451"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62613"],["dc.language.iso","en"],["dc.relation.eissn","2046-1402"],["dc.relation.issn","2046-1402"],["dc.title","Transcripts and tumors: regulatory and metabolic programming during biotrophic phytopathogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2388"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","The Plant Cell"],["dc.bibliographiccitation.lastpage","2401"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Scherer, Mario"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Starke, Verena"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2022-04-05T15:35:33Z"],["dc.date.available","2022-04-05T15:35:33Z"],["dc.date.issued","2006-09"],["dc.description.abstract","In the phytopathogenic fungus Ustilago maydis, pathogenic development is controlled by a heterodimer of the two homeodomain proteins bE and bW, encoded by the b-mating-type locus. We have identified a b-dependently induced gene, clampless1 (clp1), that is required for the proliferation of dikaryotic filaments in planta. We show that U. maydis hyphae develop structures functionally equivalent to clamp cells that participate in the distribution of nuclei during cell division. In clp1 mutant strains, dikaryotic filaments penetrate the plant cuticle, but development is stalled before the first mitotic division, and the clamp-like structures are not formed. Although clp1 is immediately activated upon b-induction on the transcriptional level, nuclear-localized Clp1 protein is first observed at the stage of plant penetration prior to the first cell division. Induced expression of clp1 strongly interferes with b-dependent gene regulation and blocks b-dependent filament formation and b-dependent cell cycle arrest. We speculate that the Clp1 protein inhibits the activity of the bE/bW heterodimer to facilitate the cell cycle progression during hyphal growth."],["dc.identifier.doi","10.1105/tpc.106.043521"],["dc.identifier.pmid","16920779"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106404"],["dc.language.iso","en"],["dc.relation.issn","1040-4651"],["dc.title","The Clp1 protein is required for clamp formation and pathogenic development of Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4262"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","The Plant Cell"],["dc.bibliographiccitation.lastpage","4277"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Freitag, Johannes"],["dc.contributor.author","Hampel, Martin"],["dc.contributor.author","Ast, Julia"],["dc.contributor.author","Boelker, Michael"],["dc.contributor.author","Kaemper, Joerg"],["dc.date.accessioned","2018-11-07T09:18:59Z"],["dc.date.available","2018-11-07T09:18:59Z"],["dc.date.issued","2013"],["dc.description.abstract","The unfolded protein response (UPR) is a conserved eukaryotic signaling pathway regulating endoplasmic reticulum (ER) homeostasis during ER stress, which results, for example, from an increased demand for protein secretion. Here, we characterize the homologs of the central UPR regulatory proteins Hac1 (for Homologous to ATF/CREB1) and Inositol Requiring Enzyme1 in the plant pathogenic fungus Ustilago maydis and demonstrate that the UPR is tightly interlinked with the b mating-type-dependent signaling pathway that regulates pathogenic development. Exact timing of UPR is required for virulence, since premature activation interferes with the b-dependent switch from budding to filamentous growth. In addition, we found crosstalk between UPR and the b target Clampless1 (Clp1), which is essential for cell cycle release and proliferation in planta. The unusual C-terminal extension of the U. maydis Hac1 homolog, Cib1 (for Clp1 interacting bZIP1), mediates direct interaction with Clp1. The interaction between Clp1 and Cib1 promotes stabilization of Clp1, resulting in enhanced ER stress tolerance that prevents deleterious UPR hyperactivation. Thus, the interaction between Cib1 and Clp1 constitutes a checkpoint to time developmental progression and increased secretion of effector proteins at the onset of biotrophic development. Crosstalk between UPR and the b mating-type regulated developmental program adapts ER homeostasis to the changing demands during biotrophy."],["dc.identifier.doi","10.1105/tpc.113.115899"],["dc.identifier.isi","000327723100047"],["dc.identifier.pmid","24179126"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28530"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Plant Biologists"],["dc.relation.issn","1532-298X"],["dc.relation.issn","1040-4651"],["dc.title","Crosstalk between the Unfolded Protein Response and Pathways That Regulate Pathogenic Development in Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1001035"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS Pathogens"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Scherer, Mario"],["dc.contributor.author","Vranes, Miroslav"],["dc.contributor.author","Wahl, Ramon"],["dc.contributor.author","Pothiratana, Chetsada"],["dc.contributor.author","Schuler, David"],["dc.contributor.author","Vincon, Volker"],["dc.contributor.author","Finkernagel, Florian"],["dc.contributor.author","Flor-Parra, Ignacio"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2022-04-05T15:35:54Z"],["dc.date.available","2022-04-05T15:35:54Z"],["dc.date.issued","2010-08-05"],["dc.description.abstract","In the phytopathogenic basidiomycete Ustilago maydis, sexual and pathogenic development are tightly connected and controlled by the heterodimeric bE/bW transcription factor complex encoded by the b-mating type locus. The formation of the active bE/bW heterodimer leads to the formation of filaments, induces a G2 cell cycle arrest, and triggers pathogenicity. Here, we identify a set of 345 bE/bW responsive genes which show altered expression during these developmental changes; several of these genes are associated with cell cycle coordination, morphogenesis and pathogenicity. 90% of the genes that show altered expression upon bE/bW-activation require the zinc finger transcription factor Rbf1, one of the few factors directly regulated by the bE/bW heterodimer. Rbf1 is a novel master regulator in a multilayered network of transcription factors that facilitates the complex regulatory traits of sexual and pathogenic development."],["dc.identifier.doi","10.1371/journal.ppat.1001035"],["dc.identifier.pmid","20700446"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106406"],["dc.language.iso","en"],["dc.relation.eissn","1553-7374"],["dc.title","The transcription factor Rbf1 is the master regulator for b-mating type controlled pathogenic development in Ustilago maydis"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1118"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Molecular Plant-Microbe Interactions"],["dc.bibliographiccitation.lastpage","1129"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Zahiri, Alexander"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Wahl, Ramon"],["dc.contributor.author","Rath, Magnus"],["dc.contributor.author","Kämper, Jörg"],["dc.date.accessioned","2019-11-13T08:34:49Z"],["dc.date.available","2019-11-13T08:34:49Z"],["dc.date.issued","2010"],["dc.description.abstract","Ustilago maydis is a plant-pathogenic fungus that establishes a biotrophic relationship with its host plant, Zea mays. The pathogenic stage of U. maydis is initiated by the fusion of two haploid cells, resulting in the formation of a dikaryotic hypha that invades the plant cell. The switch from saprophytic, yeast-like cells to the biotrophic hyphae requires the complex regulation of a multitude of biological processes to constitute the compatible host-fungus interaction. Transcriptional regulators involved in the establishment of the infectious dikaryon and penetration of the host tissue have been identified; however, regulators required during the post-penetration stages remained to be elucidated. In this study, we report the identification of a U. maydis forkhead transcription factor, Fox1, which is exclusively expressed during biotrophic development. Deletion of fox1 results in reduced virulence and impaired tumor development. The Deltafox1 hyphae induce the accumulation of H(2)O(2) in and around infected cells and a maize defense response phenotypically represented by the encasement of proliferating hyphae in a cellulose-containing matrix. The phenotype can be attributed to the fox1-dependent deregulation of several effector genes that are linked to pathogenic development and host defense suppression."],["dc.identifier.doi","10.1094/MPMI-23-9-1118"],["dc.identifier.pmid","20687802"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/62614"],["dc.language.iso","en"],["dc.relation.issn","0894-0282"],["dc.title","The Ustilago maydis forkhead transcription factor Fox1 is involved in the regulation of genes required for the attenuation of plant defenses during pathogenic development"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1135"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","New Phytologist"],["dc.bibliographiccitation.lastpage","1148"],["dc.bibliographiccitation.volume","209"],["dc.contributor.author","Lo Presti, Libera"],["dc.contributor.author","Lopez Diaz, Cristina"],["dc.contributor.author","Turra, David"],["dc.contributor.author","Di Pietro, Antonio"],["dc.contributor.author","Hampel, Martin"],["dc.contributor.author","Heimel, Kai"],["dc.contributor.author","Kahmann, Regine"],["dc.date.accessioned","2018-11-07T10:18:24Z"],["dc.date.available","2018-11-07T10:18:24Z"],["dc.date.issued","2016"],["dc.description.abstract","The maize pathogenic fungus Ustilago maydis experiences endoplasmic reticulum (ER) stress during plant colonization and relies on the unfolded protein response (UPR) to cope with this stress. We identified the U. maydis co-chaperone, designated Dnj1, as part of this conserved cellular response to ER stress Delta dnj1 cells are sensitive to the ER stressor tunicamycin and display a severe virulence defect in maize infection assays. A dnj1 mutant allele unable to stimulate the ATPase activity of chaperones phenocopies the null allele. A Dnj1-mCherry fusion protein localizes in the ER and interacts with the luminal chaperone Bip1. The Fusarium oxysporum Dnj1 ortholog contributes to the virulence of this fungal pathogen in tomato plants. Unlike the human ortholog, F. oxysporum Dnj1 partially rescues the virulence defect of the Ustilago dnj1 mutant. By enabling the fungus to restore ER homeostasis and maintain a high secretory activity, Dnj1 contributes to the establishment of a compatible interaction with the host. Dnj1 orthologs are present in many filamentous fungi, but are absent in budding and fission yeasts. We postulate a conserved and essential role during virulence for this class of co-chaperones."],["dc.identifier.doi","10.1111/nph.13703"],["dc.identifier.isi","000373378000026"],["dc.identifier.pmid","26487566"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/41436"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1469-8137"],["dc.relation.issn","0028-646X"],["dc.title","A conserved co-chaperone is required for virulence in fungal plant pathogens"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","121"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Applied Microbiology and Biotechnology"],["dc.bibliographiccitation.lastpage","132"],["dc.bibliographiccitation.volume","99"],["dc.contributor.author","Heimel, Kai"],["dc.date.accessioned","2018-11-07T10:03:50Z"],["dc.date.available","2018-11-07T10:03:50Z"],["dc.date.issued","2015"],["dc.description.abstract","The unfolded protein response (UPR) represents a mechanism to preserve endoplasmic reticulum (ER) homeostasis that is conserved in eukaryotes. ER stress caused by the accumulation of potentially toxic un- or misfolded proteins in the ER triggers UPR activation and the induction of genes important for protein folding in the ER, ER expansion, and transport from and to the ER. Along with this adaptation, the overall capacity for protein secretion is markedly increased by the UPR. In filamentous fungi, various approaches to employ the UPR for improved production of homologous and heterologous proteins have been investigated. As the effects on protein production were strongly dependent on the expressed protein, generally applicable strategies have to be developed. A combination of transcriptomic approaches monitoring secretion stress and basic research on the UPR mechanism provided novel and important insight into the complex regulatory cross-connections between UPR signalling, cellular physiology, and developmental processes. It will be discussed how this increasing knowledge on the UPR might stimulate the development of novel strategies for using the UPR as a tool in biotechnology."],["dc.identifier.doi","10.1007/s00253-014-6192-7"],["dc.identifier.isi","000347685300012"],["dc.identifier.pmid","25384707"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38565"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1432-0614"],["dc.relation.issn","0175-7598"],["dc.title","Unfolded protein response in filamentous fungi-implications in biotechnology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]