Soong, Poh Loong

[["dc.bibliographiccitation.artnumber","209"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Dai, Yuanyuan"],["dc.contributor.author","Amenov, Asset"],["dc.contributor.author","Ignatyeva, Nadezda"],["dc.contributor.author","Koschinski, Andreas"],["dc.contributor.author","Xu, Hang"],["dc.contributor.author","Soong, Poh Loong"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Linke, Wolfgang A."],["dc.contributor.author","Zaccolo, Manuela"],["dc.contributor.author","Hasenfuss, Gerd"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.contributor.author","Ebert, Antje"],["dc.date.accessioned","2020-04-14T14:41:47Z"],["dc.date.available","2020-04-14T14:41:47Z"],["dc.date.issued","2020"],["dc.description.abstract","The sarcomeric troponin-tropomyosin complex is a critical mediator of excitation-contraction coupling, sarcomeric stability and force generation. We previously reported that induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) from patients with a dilated cardiomyopathy (DCM) mutation, troponin T (TnT)-R173W, display sarcomere protein misalignment and impaired contractility. Yet it is not known how TnT mutation causes dysfunction of sarcomere microdomains and how these events contribute to misalignment of sarcomeric proteins in presence of DCM TnT-R173W. Using a human iPSC-CM model combined with CRISPR/Cas9-engineered isogenic controls, we uncovered that TnT-R173W destabilizes molecular interactions of troponin with tropomyosin, and limits binding of PKA to local sarcomere microdomains. This attenuates troponin phosphorylation and dysregulates local sarcomeric microdomains in DCM iPSC-CMs. Disrupted microdomain signaling impairs MYH7-mediated, AMPK-dependent sarcomere-cytoskeleton filament interactions and plasma membrane attachment. Small molecule-based activation of AMPK can restore TnT microdomain interactions, and partially recovers sarcomere protein misalignment as well as impaired contractility in DCM TnT-R173W iPSC-CMs. Our findings suggest a novel therapeutic direction targeting sarcomere- cytoskeleton interactions to induce sarcomere re-organization and contractile recovery in DCM."],["dc.identifier.doi","10.1038/s41598-019-56597-3"],["dc.identifier.pmid","31937807"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64087"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/336"],["dc.language.iso","en"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | A12: Alternative molekulare Signaltransduktionswege durch Kardiomyopathie-verursachende Troponin-Mutationen"],["dc.relation.issn","2045-2322"],["dc.relation.workinggroup","RG Ebert (Cardiovascular Cell Biology and Systems Medicine)"],["dc.relation.workinggroup","RG Hasenfuß (Transition zur Herzinsuffizienz)"],["dc.relation.workinggroup","RG Linke (Kardiovaskuläre Physiologie)"],["dc.relation.workinggroup","RG Tiburcy (Stem Cell Disease Modeling)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.rights","CC BY 4.0"],["dc.title","Troponin destabilization impairs sarcomere-cytoskeleton interactions in iPSC-derived cardiomyocytes from dilated cardiomyopathy patients"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","167"],["dc.bibliographiccitation.lastpage","176"],["dc.bibliographiccitation.seriesnr","1181"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Meyer, Tim"],["dc.contributor.author","Soong, Poh Loong"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.date.accessioned","2017-09-07T11:54:28Z"],["dc.date.available","2017-09-07T11:54:28Z"],["dc.date.issued","2014"],["dc.description.abstract","Cardiac muscle engineering has evolved over nearly 20 years from a scientific oddity to a mainstream technology with a wide range of applications. Of the many published methods it appears that hydrogels constitute the preferred scaffolds for myocardial tissue engineering and support of organotypic development. Here we describe a simple and highly robust protocol for the generation of engineered heart muscle using a collagen-based hydrogel method."],["dc.identifier.doi","10.1007/978-1-4939-1047-2_15"],["dc.identifier.gro","3145187"],["dc.identifier.pmid","25070336"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2895"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/63"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.status","final"],["dc.publisher","Humana Press"],["dc.publisher.place","New York"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.isbn","978-1-4939-1046-5"],["dc.relation.ispartof","Cardiac Tissue Engineering"],["dc.relation.ispartofseries","Methods in Molecular Biology;1181"],["dc.relation.workinggroup","RG Tiburcy (Stem Cell Disease Modeling)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.title","Collagen-Based Engineered Heart Muscle"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Current Protocols in Cell Biology"],["dc.contributor.author","Soong, Poh Loong"],["dc.contributor.author","Tiburcy, Malte"],["dc.contributor.author","Zimmermann, Wolfram-Hubertus"],["dc.date.accessioned","2017-09-07T11:54:28Z"],["dc.date.available","2017-09-07T11:54:28Z"],["dc.date.issued","2012"],["dc.identifier.doi","10.1002/0471143030.cb2308s55"],["dc.identifier.gro","3145185"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2893"],["dc.notes.intern","Crossref Import"],["dc.notes.status","public"],["dc.publisher","Wiley-Blackwell"],["dc.title","Cardiac Differentiation of Human Embryonic Stem Cells and their Assembly into Engineered Heart Muscle"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","P2545"],["dc.bibliographiccitation.firstpage","533"],["dc.bibliographiccitation.issue","suppl_1"],["dc.bibliographiccitation.journal","European Heart Journal"],["dc.bibliographiccitation.volume","38"],["dc.contributor.author","Soong, P. L."],["dc.contributor.author","Sur, S."],["dc.contributor.author","Fujita, B."],["dc.contributor.author","Grishina, E."],["dc.contributor.author","Kuzyakova, M."],["dc.contributor.author","Tiburcy, M."],["dc.contributor.author","Zimmermann, W. H."],["dc.date.accessioned","2019-02-27T09:52:25Z"],["dc.date.available","2019-02-27T09:52:25Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1093/eurheartj/ehx502.P2545"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/57635"],["dc.identifier.url","https://sfb1002.med.uni-goettingen.de/production/literature/publications/178"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.relation","SFB 1002: Modulatorische Einheiten bei Herzinsuffizienz"],["dc.relation","SFB 1002 | C04: Fibroblasten-Kardiomyozyten Interaktion im gesunden und erkrankten Herzen: Mechanismen und therapeutische Interventionen bei Kardiofibroblastopathien"],["dc.relation.issn","0195-668X"],["dc.relation.workinggroup","RG Tiburcy (Stem Cell Disease Modeling)"],["dc.relation.workinggroup","RG Zimmermann (Engineered Human Myocardium)"],["dc.title","Inducible paracrine release of IGF-1 improves heart muscle thickness and function"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]