Man, Kwun-nok Mimi

[["dc.bibliographiccitation.firstpage","1351"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Protocols"],["dc.bibliographiccitation.lastpage","1365"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Burgalossi, Andrea"],["dc.contributor.author","Jung, SangYong"],["dc.contributor.author","Man, Kwun-nok Mimi"],["dc.contributor.author","Nair, Ramya"],["dc.contributor.author","Jockusch, Wolf J"],["dc.contributor.author","Wojcik, Sonja M"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Rhee, Jeong-Seop"],["dc.date.accessioned","2017-09-07T11:48:50Z"],["dc.date.available","2017-09-07T11:48:50Z"],["dc.date.issued","2012"],["dc.description.abstract","Neurotransmitter release is triggered by membrane depolarization, Ca²⁺ influx and Ca²⁺ sensing by the release machinery, causing synaptic vesicle (SV) fusion with the plasma membrane. Interlinked is a complex membrane cycle in which vesicles are tethered to the release site, primed, fused and recycled. As many of these processes are Ca²⁺ dependent and simultaneously occurring, it is difficult to dissect them experimentally. This problem can be partially circumvented by controlling synaptic Ca²⁺ concentrations via UV photolysis of caged Ca²⁺. We developed a culture protocol for Ca²⁺ uncaging in small synapses on the basis of the generation of small glia cell islands with single neurons on top, which are sufficiently small to be covered with a UV-light flash. Neurons are loaded with the photolabile Ca²⁺-chelator nitrophenyl-EGTA and Ca²⁺ indicators, and a UV flash is used to trigger Ca²⁺-uncaging and SV fusion. The protocol takes three weeks to complete and provides unprecedented insights into the mechanisms of transmitter release."],["dc.identifier.doi","10.1038/nprot.2012.074"],["dc.identifier.gro","3142502"],["dc.identifier.isi","000305960400008"],["dc.identifier.pmid","22722370"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8860"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1754-2189"],["dc.title","Analysis of neurotransmitter release mechanisms by photolysis of caged Ca²⁺ in an autaptic neuron culture system"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","135"],["dc.bibliographiccitation.journal","Molecular Medicine"],["dc.bibliographiccitation.lastpage","148"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Wojcik, Sonja M."],["dc.contributor.author","Tantra, Martesa"],["dc.contributor.author","Stepniak, Beata"],["dc.contributor.author","Man, Kwun-nok M"],["dc.contributor.author","Müller-Ribbe, Katja"],["dc.contributor.author","Begemann, Martin"],["dc.contributor.author","Ju, Anes"],["dc.contributor.author","Papiol, Sergi"],["dc.contributor.author","Ronnenberg, Anja"],["dc.contributor.author","Gurvich, Artem"],["dc.contributor.author","Shin, Yong"],["dc.contributor.author","Augustin, Iris"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Ehrenreich, Hannelore"],["dc.date.accessioned","2017-09-07T11:46:37Z"],["dc.date.available","2017-09-07T11:46:37Z"],["dc.date.issued","2013"],["dc.description.abstract","Anxiety disorders and substance abuse, including benzodiazepine use disorder, frequently occur together. Unfortunately, treatment of anxiety disorders still includes benzodiazepines, and patients with an existing comorbid benzodiazepine use disorder or a genetic susceptibility for benzodiazepine use disorder may be at risk of adverse treatment outcomes. The identification of genetic predictors for anxiety disorders, and especially for benzodiazepine use disorder, could aid the selection of the best treatment option and improve clinical outcomes. The brain-specific angiogenesis inhibitor I-associated protein 3 (Baiap3) is a member of the mammalian uncoordinated 13 (Munc13) protein family of synaptic regulators of neurotransmitter exocytosis, with a striking expression pattern in amygdalae, hypothalamus and periaqueductal gray. Deletion of Baiap3 in mice leads to enhanced seizure propensity and increased anxiety, with the latter being more pronounced in female than in male animals. We hypothesized that genetic variation in human BAIAP3 may also be associated with anxiety. By using a phenotype-based genetic association study, we identified two human BAIAP3 single-nucleotide polymorphism risk genotypes (AA for rs2235632, TT for rs1132358) that show a significant association with anxiety in women and, surprisingly, with benzodiazepine abuse in men. Returning to mice, we found that male, but not female, Baiap3 knockout (KO) mice develop tolerance to diazepam more quickly than control animals. Analysis of cultured Baiap3 KO hypothalamus slices revealed an increase in basal network activity and an altered response to diazepam withdrawal. Thus, Baiap3/BAIAP3 is gender specifically associated with anxiety and benzodiazepine use disorder, and the analysis of Baiap3/BAIAP3-related functions may help elucidate mechanisms underlying the development of both disorders."],["dc.identifier.doi","10.2119/molmed.2013.00033"],["dc.identifier.gro","3150563"],["dc.identifier.pmid","23698091"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7337"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Genetic Markers of a Munc13 Protein Family Member, BAIAP3, Are Gender Specifically Associated with Anxiety and Benzodiazepine Abuse in Mice and Humans"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1688"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.lastpage","1700"],["dc.bibliographiccitation.volume","28"],["dc.contributor.author","Chehab, Tarek"],["dc.contributor.author","Santos, Nina Criado"],["dc.contributor.author","Holthenrich, Anna"],["dc.contributor.author","Koerdt, Sophia N."],["dc.contributor.author","Disse, Jennifer"],["dc.contributor.author","Schuberth, Christian"],["dc.contributor.author","Nazmi, Ali Reza"],["dc.contributor.author","Neeft, Maaike"],["dc.contributor.author","Koch, Henriette"],["dc.contributor.author","Man, Kwun Nok M."],["dc.contributor.author","Wojcik, Sonja M."],["dc.contributor.author","Martin, Thomas F. J."],["dc.contributor.author","van der Sluijs, Peter"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Gerke, Volker"],["dc.contributor.editor","Mostov, Keith E."],["dc.date.accessioned","2018-03-08T09:21:31Z"],["dc.date.available","2018-03-08T09:21:31Z"],["dc.date.issued","2017"],["dc.description.abstract","Endothelial cells respond to blood vessel injury by the acute release of the procoagulant von Willebrand factor, which is stored in unique secretory granules called Weibel–Palade bodies (WPBs). Stimulated WPB exocytosis critically depends on their proper recruitment to the plasma membrane, but factors involved in WPB–plasma membrane tethering are not known. Here we identify Munc13-4, a protein mutated in familial hemophagocytic lymphohistiocytosis 3, as a WPB-tethering factor. Munc13-4 promotes histamine-evoked WPB exocytosis and is present on WPBs, and secretagogue stimulation triggers an increased recruitment of Munc13-4 to WPBs and a clustering of Munc13-4 at sites of WPB–plasma membrane contact. We also identify the S100A10 subunit of the annexin A2 (AnxA2)-S100A10 protein complex as a novel Munc13-4 interactor and show that AnxA2-S100A10 participates in recruiting Munc13-4 to WPB fusion sites. These findings indicate that Munc13-4 supports acute WPB exocytosis by tethering WPBs to the plasma membrane via AnxA2-S100A10."],["dc.identifier.doi","10.1091/mbc.E17-02-0128"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/12902"],["dc.language.iso","en"],["dc.notes.intern","GRO-Li-Import"],["dc.notes.status","final"],["dc.relation.doi","10.1091/mbc.E17-02-0128"],["dc.relation.issn","1059-1524"],["dc.relation.issn","1939-4586"],["dc.title","A novel Munc13-4/S100A10/annexin A2 complex promotes Weibel–Palade body exocytosis in endothelial cells"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e10635"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Man, Kwun Nok M"],["dc.contributor.author","Imig, Cordelia"],["dc.contributor.author","Walter, Alexander M"],["dc.contributor.author","Pinheiro, Paulo S"],["dc.contributor.author","Stevens, David R"],["dc.contributor.author","Rettig, Jens"],["dc.contributor.author","Sørensen, Jakob B"],["dc.contributor.author","Cooper, Benjamin H"],["dc.contributor.author","Brose, Nils"],["dc.contributor.author","Wojcik, Sonja M"],["dc.date.accessioned","2017-09-07T11:54:53Z"],["dc.date.available","2017-09-07T11:54:53Z"],["dc.date.issued","2015"],["dc.description.abstract","It is currently unknown whether the molecular steps of large dense-core vesicle (LDCV) docking and priming are identical to the corresponding reactions in synaptic vesicle (SV) exocytosis. Munc13s are essential for SV docking and priming, and we systematically analyzed their role in LDCV exocytosis using chromaffin cells lacking individual isoforms. We show that particularly Munc13-2 plays a fundamental role in LDCV exocytosis, but in contrast to synapses lacking Munc13s, the corresponding chromaffin cells do not exhibit a vesicle docking defect. We further demonstrate that ubMunc13-2 and Munc13-1 confer Ca2+-dependent LDCV priming with similar affinities, but distinct kinetics. Using a mathematical model, we identify an early LDCV priming step that is strongly dependent upon Munc13s. Our data demonstrate that the molecular steps of SV and LDCV priming are very similar while SV and LDCV docking mechanisms are distinct."],["dc.format.extent","28"],["dc.identifier.doi","10.7554/eLife.10635"],["dc.identifier.gro","3141787"],["dc.identifier.isi","000373851200001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1068"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: Max-Planck-Gesellschaft; Deutsche Forschungsgemeinschaft"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2050-084X"],["dc.title","Identification of a Munc13-sensitive step in chromaffin cell large dense-core vesicle exocytosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]