Lippert, Undine

[["dc.bibliographiccitation.firstpage","520"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Experimental Dermatology"],["dc.bibliographiccitation.lastpage","525"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Henz, B. M."],["dc.contributor.author","Neumann, C."],["dc.date.accessioned","2018-11-07T10:46:44Z"],["dc.date.available","2018-11-07T10:46:44Z"],["dc.date.issued","2004"],["dc.description.abstract","CXCL8 plays a major role in cell recruitment to sites of inflammation. Apart from neutrophils, little is known, however, about the cellular distribution and regulation of CXCL8 receptors in cells involved in acquired and adaptive immune responses. In previous studies, we have demonstrated the extracellular expression and function of CXCR1/2 on mast cells and also detected an intracellular pool of CXCR1/2. Here, we have addressed the question of receptor regulation during stimulation of human mast cells (HMC-1 cell line) and have studied T cells in comparison. Cell permeabilization was performed to detect both surface and possible intracellular receptor pools. HMC-1 cells stained positive for both receptors on the cell surface (CXCR1, 50%; CXCR2, 51%) and also after cell permeabilization (CXCR1, 86%; CXCR2, 74%). Similarly, T cells exhibited both cell-surface receptor expression (CXCR1, 30%; CXCR2, 23%) and higher total receptor expression (CXCR1, 50%; CXCR2, 36%), although overall values were lower than that in HMC-1 cells. On immunoblot, molecular weights of extra- and intracellular receptors on mast cells were the same, excluding altered receptor glycosylation. On stimulation with phorbol 12-myristate 13-acetate plus calcium ionophore, a time-dependent decrease of surface-membrane receptors was observed in both cell types, while total receptor remained the same, suggesting that receptor shedding is not involved. The kinetics of membrane receptor internalization and replenishment differed for the two cell types. Furthermore, receptor internalization was associated with decreased F-actin polymerization, a basic prerequisite for cell migration. These findings demonstrate for the first time the expression of extra- and intracellular CXCR1/2 receptors on T cells and delineate the dynamics of CXCR1/2 receptors on mast cells and T cells. Furthermore, they suggest a cell-type-specific and finely tuned regulation of chemokine responses at the receptor level in the context of inflammation."],["dc.identifier.doi","10.1111/j.0906-6705.2004.00182.x"],["dc.identifier.isi","000222717800008"],["dc.identifier.pmid","15265017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47811"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Munksgaard"],["dc.relation.issn","0906-6705"],["dc.title","Human T lymphocytes and mast cells differentially express and regulate extra- and intracellular CXCR1 and CXCR2"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","116"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Investigative Dermatology"],["dc.bibliographiccitation.lastpage","123"],["dc.bibliographiccitation.volume","123"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Artuc, M."],["dc.contributor.author","Grutzkau, A."],["dc.contributor.author","Babina, M."],["dc.contributor.author","Guhl, S."],["dc.contributor.author","Haase, I."],["dc.contributor.author","Blaschke, V."],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Knosalla, M."],["dc.contributor.author","Middel, Peter"],["dc.contributor.author","Kruger-Krasagakis, S."],["dc.contributor.author","Henz, B. M."],["dc.date.accessioned","2018-11-07T10:47:44Z"],["dc.date.available","2018-11-07T10:47:44Z"],["dc.date.issued","2004"],["dc.description.abstract","Mast cells generate and release histamine during anaphylactic reactions, and there is pharmacological evidence that histamine regulates this process via specific receptors. Therefore, we examined human leukemic (HMC-1) and normal skin mast cells for the expression of all four currently known histamine receptors. Both cell types expressed H2 and H4 receptors at mRNA and protein levels, whereas H3 receptor specific mRNA and receptor protein was undetectable. Similarly, immunohistochemistry of cutaneous tissue showed an absence of H3 receptor in these cells. Despite transcription of mRNA, H1 receptor protein was only moderately expressed in HMC-1 cells and was virtually absent in skin mast cells. Furthermore, only H1, H2, and H4 receptors were detectable by Western blot analysis of HMC-1 cells. Radiolabeled histamine binding was strongly inhibited only by H2 (ranitidine)- and H3/H4 (FUB 108)-specific antagonists. Histamine-induced increase of cAMP was inhibited by the H2 receptor antagonist famotidine, whereas induction of IP3 was not observed, making signaling via the H1 receptor unlikely. These data show that human mast cells constitutively express primarily H2 and H4 receptors and that H2 receptors are functionally linked to cellular processes. They provide new insights into the mechanisms that govern auto- and paracrine histamine-induced mast cell functions."],["dc.identifier.doi","10.1111/j.0022-202X.2004.22721.x"],["dc.identifier.isi","000221974400019"],["dc.identifier.pmid","15191551"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48031"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing Inc"],["dc.relation.issn","0022-202X"],["dc.title","Human skin mast cells express H2 and H4, but not H3 receptors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","European Journal of Immunology"],["dc.bibliographiccitation.volume","38"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Schwarz, Herbert"],["dc.contributor.author","Brunner, Edgar"],["dc.contributor.author","Latif, A. H. M. Mahbub"],["dc.contributor.author","Neumann, Christine"],["dc.contributor.author","Soruri, Afsaneh"],["dc.date.accessioned","2018-11-07T11:14:25Z"],["dc.date.available","2018-11-07T11:14:25Z"],["dc.date.issued","2008"],["dc.format.extent","1767"],["dc.identifier.doi","10.1002/eji.200737800"],["dc.identifier.isi","000256762400030"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54117"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-v C H Verlag Gmbh"],["dc.relation.haserratum","/handle/2/97655"],["dc.relation.issn","0014-2980"],["dc.title","CD137 ligand reverse signaling has multiple functions in human dendritic cells during an adaptive immune response (vol 38, pg 1024, 2008)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Investigative Dermatology"],["dc.bibliographiccitation.volume","125"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Brunner, E."],["dc.contributor.author","Lafti, A. H."],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Neumann, C."],["dc.contributor.author","Soruri, Afsaneh"],["dc.date.accessioned","2018-11-07T10:56:17Z"],["dc.date.available","2018-11-07T10:56:17Z"],["dc.date.issued","2005"],["dc.format.extent","A49"],["dc.identifier.isi","000231862600286"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49976"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","New york"],["dc.relation.conference","35th Annual Meeting of the European-Society-for-Dermatological-Research"],["dc.relation.eventlocation","Tubingen, GERMANY"],["dc.relation.issn","0022-202X"],["dc.title","CD137 ligand mediated differentiation of immature human dendritic cells is associated with enhanced (antigen specific) T cell activation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Experimental Dermatology"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Schwarz, H."],["dc.contributor.author","Brunner, E."],["dc.contributor.author","Latif, A. H. M. Mahbub"],["dc.contributor.author","Neumann, C."],["dc.contributor.author","Soruri, Afsaneh"],["dc.date.accessioned","2018-11-07T10:12:05Z"],["dc.date.available","2018-11-07T10:12:05Z"],["dc.date.issued","2006"],["dc.format.extent","201"],["dc.identifier.isi","000235370600050"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40169"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","33rd Annual Meeting of the Arbeitsgemeinschaft-Dermatologische-Forschung (ADF)"],["dc.relation.eventlocation","Aachen, GERMANY"],["dc.relation.issn","0906-6705"],["dc.title","CD137 ligand induced differentiation of human dendritic cells to potent activators of antigen-specific T cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Experimental Dermatology"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Babina, M."],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Schwarz, H."],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Knosalla, M."],["dc.contributor.author","Middel, Peter"],["dc.contributor.author","Neumann, C."],["dc.date.accessioned","2018-11-07T11:04:24Z"],["dc.date.available","2018-11-07T11:04:24Z"],["dc.date.issued","2007"],["dc.format.extent","226"],["dc.identifier.isi","000244056600108"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51836"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","34th Annual Meeting of the Arbeitsgemeinschaft-Dermatologische-Forschung"],["dc.relation.eventlocation","Freiburg, GERMANY"],["dc.relation.issn","0906-6705"],["dc.title","Expression and function of CD137 receptor and ligand in human mast cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","41"],["dc.bibliographiccitation.journal","Allergy"],["dc.bibliographiccitation.lastpage","42"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Schwarz, H."],["dc.contributor.author","Neumann, C."],["dc.contributor.author","Brunner, E."],["dc.contributor.author","Ul-Latif, A."],["dc.contributor.author","Soruri, Afsaneh"],["dc.date.accessioned","2018-11-07T11:01:31Z"],["dc.date.available","2018-11-07T11:01:31Z"],["dc.date.issued","2007"],["dc.identifier.isi","000246800000102"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51169"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Malden"],["dc.relation.conference","26th Congress of the European-Academy-of-Allergology-and-Clinical-Immunology"],["dc.relation.eventlocation","Gothenburg, SWEDEN"],["dc.relation.issn","0105-4538"],["dc.title","Possible bidirectional function of CD137 ligand expressed on dendritic cells in antigen specific T cell activation"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Experimental Dermatology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Schwarz, H."],["dc.contributor.author","Neumann, C."],["dc.contributor.author","Soruri, Afsaneh"],["dc.date.accessioned","2018-11-07T11:17:36Z"],["dc.date.available","2018-11-07T11:17:36Z"],["dc.date.issued","2008"],["dc.format.extent","249"],["dc.identifier.isi","000252897700058"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54845"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.publisher.place","Oxford"],["dc.relation.conference","35th Annual Meeting of the Arbeitsgemeinschaft-Dermatolog-Forschung"],["dc.relation.eventlocation","Eriangen, GERMANY"],["dc.relation.issn","0906-6705"],["dc.title","CD137 ligand induces dendritic cell maturation and migration via TNF-alpha? and CCR7"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Journal of Investigative Dermatology"],["dc.bibliographiccitation.volume","126"],["dc.contributor.author","Lippert, Undine"],["dc.contributor.author","Peiser, M."],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Ferrari, David M."],["dc.contributor.author","Knosalla, M."],["dc.contributor.author","Middel, Peter"],["dc.contributor.author","Neumann, C. H."],["dc.date.accessioned","2018-11-07T09:29:11Z"],["dc.date.available","2018-11-07T09:29:11Z"],["dc.date.issued","2006"],["dc.format.extent","62"],["dc.identifier.isi","000242891900357"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/30958"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.publisher.place","New york"],["dc.relation.conference","36th Annual Meeting of the European-Society-of-Dermatology-Research (ESDR)"],["dc.relation.eventlocation","Paris, FRANCE"],["dc.relation.issn","0022-202X"],["dc.title","Expression and function of CXCR1 and CXCR2 in human epidermal langerhans cells"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Experimental Dermatology"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Knosalla, M."],["dc.contributor.author","Zachmann, Karolin"],["dc.contributor.author","Schoen, Michael Peter"],["dc.contributor.author","Neumann, C."],["dc.contributor.author","Lippert, Undine"],["dc.date.accessioned","2018-11-07T08:32:01Z"],["dc.date.available","2018-11-07T08:32:01Z"],["dc.date.issued","2009"],["dc.format.extent","281"],["dc.identifier.isi","000263520200055"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17250"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.publisher.place","Malden"],["dc.relation.conference","36th Annual Meeting of the Arbeitsgemeinschaft-Dermatologishche-Forschung"],["dc.relation.eventlocation","Heidelberg, GERMANY"],["dc.relation.issn","0906-6705"],["dc.title","T cells in psoriasis lesions lack ICOS expression"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]