Miosge, Nicolai

[["dc.bibliographiccitation.firstpage","1399"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Expert Opinion on Biological Therapy"],["dc.bibliographiccitation.lastpage","1405"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-11-07T11:22:40Z"],["dc.date.available","2018-11-07T11:22:40Z"],["dc.date.issued","2009"],["dc.description.abstract","Enhancing the regeneration potential of hyaline cartilage tissue remains a great challenge. During embryonic development, some of the cells of the inner cell mass will turn into the mesoderm. This will be the founder of the mesenchymal cells in connective tissues of adult life, such as bone, tendon, muscle, and cartilage. Some of these embryonic mesenchymal cells are believed not to differentiate, but reside in each of the tissues. These are now collectively described as adult mesenchymal stem cells, which are thought to be capable of repairing injured tissue. We will briefly summarize the current knowledge about stem cell-related cells in cartilage tissue and carefully discuss the potential of the cell population we described recently as a starting-point for a regenerative therapy for osteoarthritis. We found that repair tissue from human articular cartilage during the late stages of osteoarthritis harbors a unique progenitor cell population, termed chondrogenic progenitor cells (CPC). These exhibit stem cell characteristics combined with a high chondrogenic potential. They offer new insights into the biology of progenitor cells and may be relevant in the development of novel therapeutic approaches for a cell-based therapy for late stages of osteoarthritis."],["dc.identifier.doi","10.1517/14712590903246370"],["dc.identifier.isi","000271454300005"],["dc.identifier.pmid","19793003"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6059"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56025"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Informa Healthcare"],["dc.relation.issn","1471-2598"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Stem cell therapy for cartilage regeneration in osteoarthritis"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1342"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Journal of Cellular Biochemistry"],["dc.bibliographiccitation.lastpage","1355"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Ponce, M. L."],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Kluever, A."],["dc.contributor.author","Heinemann, D. E. H."],["dc.contributor.author","Miosge, Nicolai"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Frosch, Karl-Heinz"],["dc.contributor.author","Schuetze, N."],["dc.contributor.author","Hufner, A."],["dc.contributor.author","Siggelkow, Heide"],["dc.date.accessioned","2018-11-07T11:13:34Z"],["dc.date.available","2018-11-07T11:13:34Z"],["dc.date.issued","2008"],["dc.description.abstract","Knowledge of the basic mechanisms controlling osteogenesis and adipogenesis might provide new insights into the prevention of osteoporosis and age-related osteopenia. With the help of magnetic cell sorting and fluorescence activated cell sorting (FACS), osteoblastic subpopulations of mesenchymal progenitor cells were characterized. Alkaline phosphatase (AP) negative cells expressed low levels of osteoblastic and adipocytic markers. AP positive cells expressed adipocytic markers more strongly than the AP negative cell populations, thus suggesting that committed osteoblasts exhibit a greater adipogenic potential. AP negative cells differentiated to the mature osteoblastic phenotype, as demonstrated by increased AP-activity and osteocalcin secretion under standard osteogenic culture conditions. Surprisingly, this was accompanied by increased expression of adipocytic gene markers such as peroxisome proliferator-activated receptor-gamma 2, lipoprotein lipase and fatty acid binding protein. The induction of adipogenic markers was suppressed by transforming growth factor-beta 1 (TGF-beta 1) and promoted by bone morphogenetic protein 2 (BMP-2). Osteogenic culture conditions including BMP-2 induced both the formation of mineralized nodules and cytoplasmic lipid vacuoles. Upon immunogold electron microscopic analysis, osteoblastic and adipogenic marker proteins were detectable in the same cell. Our results suggest that osteogenic and adipogenic differentiation in human mesenchymal progenitor cells might not be exclusively reciprocal, but rather, a parallel event until late during osteoblast development."],["dc.identifier.doi","10.1002/jcb.21711"],["dc.identifier.isi","000257567300018"],["dc.identifier.pmid","18286543"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6248"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53928"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0730-2312"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Coexpression of osteogenic and adipogenic differentiation markers in selected subpopulations of primary human mesenchymal progenitor cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Arthritis & Rheumatism"],["dc.bibliographiccitation.volume","64"],["dc.contributor.author","Blaschke, Sabine"],["dc.contributor.author","Trautmann, Sandra"],["dc.contributor.author","Beham, Alexander W."],["dc.contributor.author","Mai, Burkhard"],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Breysach, Caroline"],["dc.contributor.author","Wolf, Gabriele"],["dc.contributor.author","Mueller, Georg Anton"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-11-07T09:04:58Z"],["dc.date.available","2018-11-07T09:04:58Z"],["dc.date.issued","2012"],["dc.format.extent","S517"],["dc.identifier.isi","000309748302284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25220"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.publisher.place","Hoboken"],["dc.relation.conference","Annual Scientific Meeting of the American-College-of-Rheumatology (ACR) and Association-of-Rheumatology-Health-Professionals (ARHP)"],["dc.relation.eventlocation","Washington, DC"],["dc.relation.issn","0004-3591"],["dc.title","Functional Role of Chondrogenic Progenitor Cells in Rheumatoid Arthritis."],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","R56"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Arthritis Research & Therapy"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Clauditz, Till Sebastian"],["dc.contributor.author","Kaste, Matthias"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-11-07T10:29:52Z"],["dc.date.available","2018-11-07T10:29:52Z"],["dc.date.issued","2006"],["dc.description.abstract","As a member of the thrombospondin gene family, cartilage oligomeric protein ( COMP) is found mainly in the extracellular matrix often associated with cartilage tissue. COMP exhibits a wide binding repertoire and has been shown to be involved in the regulation of chondrogenesis in vitro. Not much is known about the role of COMP in human cartilage tissue in vivo. With the help of immunohistochemistry, Western blot, in situ hybridization, and real-time reverse transcription-polymerase chain reaction, we aimed to elucidate the role of COMP in human embryonic, adult healthy, and osteoarthritis (OA) cartilage tissue. COMP is present during the earliest stages of human limb maturation and is later found in regions where the joints develop. In healthy and diseased cartilage tissue, COMP is secreted by the chondrocytes and is often associated with the collagen fibers. In late stages of OA, five times the COMP mRNA is produced by chondrocytes found in an area adjacent to the main defect than in an area with macroscopically normal appearance. The results indicate that COMP might be involved in human limb development, is upregulated in OA, and due to its wide binding repertoire, could play a role in the pathogenesis of OA as a factor secreted by chondrocytes to ameliorate the matrix breakdown."],["dc.identifier.doi","10.1186/ar1922"],["dc.identifier.isi","000237649000008"],["dc.identifier.pmid","16542502"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/1367"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43735"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1478-6354"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Cartilage oligomeric matrix protein is involved in human limb development and in the pathogenesis of osteoarthritis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","S205"],["dc.bibliographiccitation.issue","Suppl B"],["dc.bibliographiccitation.journal","Osteoarthritis and Cartilage"],["dc.bibliographiccitation.lastpage","S206"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Koelling, S."],["dc.contributor.author","Kruegel, J."],["dc.contributor.author","Lochte, T."],["dc.contributor.author","Miosge, N."],["dc.date.accessioned","2018-08-20T12:10:57Z"],["dc.date.available","2018-08-20T12:10:57Z"],["dc.date.issued","2006"],["dc.identifier.doi","10.1016/S1063-4584(07)60833-4"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15424"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.relation.eissn","1063-4584"],["dc.title","P389 Chrondrogenic Progenitorcells derived from late stages of Human Osteoarthirtis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","329"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Scandinavian Journal of Medicine and Science in Sports"],["dc.bibliographiccitation.lastpage","337"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Klinger, H.-M."],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Baums, Mike Herbert"],["dc.contributor.author","Kahl, E."],["dc.contributor.author","Steckel, Hanno"],["dc.contributor.author","Smith, M. Mitchell"],["dc.contributor.author","Schultz, Wolfgang"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-11-07T08:29:38Z"],["dc.date.available","2018-11-07T08:29:38Z"],["dc.date.issued","2009"],["dc.description.abstract","Our objective was to evaluate the cell biology and biomechanical aspects of the healing process after two different techniques in open rotator cuff surgery - double-loaded bio-absorbable suture anchors combined with so-called arthroscopic Mason-Allen stitches (AAMA) and a trans-osseous suture technique combined with traditional modified Mason-Allen stitches (SMMA). Thirty-six mature sheep were randomized into two repair groups. After 6, 12, or 26 weeks, evaluation of the reinsertion site of the infraspinatus tendon was performed. The mechanical load-to-failure and stiffness results did not indicate a significant difference between the two groups. After 26 weeks, fibrocartilage was sparse in the AAMA group, whereas the SMMA group showed the most pronounced amount of fibrocartilage. We found no ultrastructural differences in collagen fiber organization between the two groups. The relative expression of collagen type II mRNA in the normal group was 1.11. For the AAMA group, 6 weeks after surgery, the relative expression was 55.47, whereas for the SMMA group it was 1.90. This in vivo study showed that the AAMA group exhibited a tendon-to-bone healing process more favorable in its cell biology than that of the traditional SMMA technique. Therefore, the AAMA technique might also be more appropriate for arthroscopic repair."],["dc.identifier.doi","10.1111/j.1600-0838.2008.00791.x"],["dc.identifier.isi","000266431300005"],["dc.identifier.pmid","18397194"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6178"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16696"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","0905-7188"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Cell biological and biomechanical evaluation of two different fixation techniques for rotator cuff repair"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Human Gene Therapy"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Miro, Xavier"],["dc.contributor.author","Urlaub, Henning"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-11-07T11:22:42Z"],["dc.date.available","2018-11-07T11:22:42Z"],["dc.date.issued","2009"],["dc.format.extent","1403"],["dc.identifier.isi","000271441000164"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56034"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Mary Ann Liebert Inc"],["dc.publisher.place","New rochelle"],["dc.relation.conference","Combined Meeting of the 17th European-Society-of-Gene-and-Cell-Therapy/16th German-Society-for-Gene-Therapy/4th German-Society-for-Stem-Cell-Research"],["dc.relation.eventlocation","Hannover, GERMANY"],["dc.relation.issn","1043-0342"],["dc.title","Migratory chondrogenic progenitor cells from late stages of osteoarthritis exhibit gender differences"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1077"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Arthritis and Rheumatism"],["dc.bibliographiccitation.lastpage","1087"],["dc.bibliographiccitation.volume","62"],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-08-20T11:44:22Z"],["dc.date.available","2018-08-20T11:44:22Z"],["dc.date.issued","2010"],["dc.description.abstract","Osteoarthritis (OA), a mainly degenerative disease, is known to be multifactorial in origin. Gene expression patterns vary between populations and sexes. Sex hormone receptors have been described in the cartilage tissue of animals and humans. We undertook this study to determine whether the regenerative potential of chondrogenic progenitor cells (CPCs) present in the arthritic tissue during the late stages of human OA might also be subject to sex-specific differences and influenced by sex steroids."],["dc.identifier.doi","10.1002/art.27311"],["dc.identifier.pmid","20131243"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6058"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15419"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1529-0131"],["dc.relation.eissn","0004-3591"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Sex differences of chondrogenic progenitor cells in late stages of osteoarthritis"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","324"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Cell Stem Cell"],["dc.bibliographiccitation.lastpage","335"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Koelling, Sebastian"],["dc.contributor.author","Kruegel, Jenny"],["dc.contributor.author","Irmer, Malte"],["dc.contributor.author","Path, Jan Ragnar"],["dc.contributor.author","Sadowski, Boguslawa"],["dc.contributor.author","Miro, Xavier"],["dc.contributor.author","Miosge, Nicolai"],["dc.date.accessioned","2018-11-07T08:30:48Z"],["dc.date.available","2018-11-07T08:30:48Z"],["dc.date.issued","2009"],["dc.description.abstract","The regeneration of diseased hyaline cartilage continues to be a great challenge, mainly because degeneration-caused either by major injury or by age-related processes-can overextend the tissue's self-renewal capacity. We show that repair tissue from human articular cartilage during the late stages of osteoarthritis harbors a unique progenitor cell population, termed chondrogenic progenitor cells (CPCs). These exhibit stem cell characteristics such as clonogenicity, multipotency, and migratory activity. The isolated CPCs, which exhibit a high chondrogenic potential, were shown to populate diseased tissue ex vivo. Moreover, downregulation of the osteogenic transcription factor runx-2 enhanced the expression of the chondrogenic transcription factor sox-9. This, in turn, increased the matrix synthesis potential of the CPCs without altering their migratory capacity. Our results offer new insights into the biology of progenitor cells in the context of diseased cartilage tissue. Our work may be relevant in the development of novel therapeutics for the later stages of osteoarthritis."],["dc.description.sponsorship","Deutsche Arthrose Stiftung; Medical Faculty, Goettingen University"],["dc.identifier.doi","10.1016/j.stem.2009.01.015"],["dc.identifier.isi","000265162700009"],["dc.identifier.pmid","19341622"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6060"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16977"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1934-5909"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Migratory Chondrogenic Progenitor Cells from Repair Tissue during the Later Stages of Human Osteoarthritis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]