Heyde, Silvia von der

[["dc.bibliographiccitation.artnumber","381"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Heyde, Silvia von der"],["dc.contributor.author","Fromm-Dornieden, Carolin"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Baumgartner, Bernhard G."],["dc.date.accessioned","2018-11-07T09:40:11Z"],["dc.date.available","2018-11-07T09:40:11Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Adipogenesis is a complex process, in which immature pre-adipocytes change morphology, micro-anatomy and physiology to become mature adipocytes. These store and accumulate fat and release diverse hormones. Massive changes in protein content and protein composition of the transforming cell take place within a short time-frame. In a previous study we analyzed changes in the abundance of free and polysomal, i. e. ribosome bound, RNAs in the first hours of adipogenesis in the murine cell line 3T3-L1. Here we analyze changes of mRNA levels and their potential contribution to the changing protein pool by determination of mRNA levels and ribosome binding to mRNAs in 3T3-L1 cells stimulated for adipogenesis. We grouped mRNA species into categories with respect to up-or down-regulated transcription and translation and analyzed the groups regarding specific functionalities based on Gene Ontology (GO). Results: A shift towards up-regulation of gene expression in early adipogenesis was detected. Genes up-regulated at the transcriptional (TC: up) and translational (TL: up) level (TC: up/ TL: up) are very likely involved in control and logistics of translation. Many of them are known to contain a TOP motif. In the TC: up/ TL: unchanged group we detected most of the metal binding proteins and metal transporters. In the TC: unchanged/ TL: up group several factors of the olfactory receptor family were identified, while in TC: unchanged/ TL: down methylation and repair genes are represented. In the TC: down/ TL: up group we detected many signaling factors. The TC: down/ TL: unchanged group mainly consists of regulatory factors. Conclusions: Within the first hours of adipogenesis, changes in transcriptional and translational regulation take place. Notably, genes with a specific biological or molecular function tend to cluster in groups according to their transcriptional and translational regulation."],["dc.identifier.doi","10.1186/1471-2164-15-381"],["dc.identifier.isi","000336678000001"],["dc.identifier.pmid","24886538"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10147"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33450"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Dynamics of mRNA and polysomal abundance in early 3T3-L1 adipogenesis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","660"],["dc.bibliographiccitation.issue","17-18"],["dc.bibliographiccitation.journal","Wiener klinische Wochenschrift"],["dc.bibliographiccitation.lastpage","661"],["dc.bibliographiccitation.volume","124"],["dc.contributor.author","Fromm-Dornieden, Carolin"],["dc.contributor.author","Heyde, Silvia von der"],["dc.contributor.author","Lytovchenko, Oleksandr"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Brenig, Bertram B."],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Baumgartner, Bernhard G."],["dc.date.accessioned","2018-11-07T09:06:27Z"],["dc.date.available","2018-11-07T09:06:27Z"],["dc.date.issued","2012"],["dc.identifier.isi","000309233400021"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25562"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Wien"],["dc.title","Identification of new translationally regulated genes in early Adipogenesis"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","9"],["dc.bibliographiccitation.journal","BMC Molecular Biology"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Fromm-Dornieden, Carolin"],["dc.contributor.author","Heyde, Silvia von der"],["dc.contributor.author","Lytovchenko, Oleksandr"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Brenig, Bertram B."],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Baumgartner, Bernhard G."],["dc.date.accessioned","2018-11-07T09:12:11Z"],["dc.date.available","2018-11-07T09:12:11Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: Control of translation allows for rapid adaptation of the cell to stimuli, rather than the slower transcriptional control. We presume that translational control is an essential process in the control of adipogenesis, especially in the first hours after hormonal stimulation. 3T3-L1 preadipocytes were cultured to confluency and adipogenesis was induced by standard protocols using a hormonal cocktail. Cells were harvested before and 6 hours after hormonal induction. mRNAs attached to ribosomes (polysomal mRNAs) were separated from unbound mRNAs by velocity sedimentation. Pools of polysomal and unbound mRNA fractions were analyzed by microarray analysis. Changes in relative abundance in unbound and polysomal mRNA pools were calculated to detect putative changes in translational activity. Changes of expression levels of selected genes were verified by qPCR and Western blotting. Results: We identified 43 genes that shifted towards the polysomal fraction (up-regulated) and 2 genes that shifted towards free mRNA fraction (down-regulated). Interestingly, we found Ghrelin to be down-regulated. Upregulated genes comprise factors that are nucleic acid binding (eIF4B, HSF1, IRF6, MYC, POLR2a, RPL18, RPL27a, RPL6, RPL7a, RPS18, RPSa, TSC22d3), form part of ribosomes (RPL18, RPL27a, RPL6, RPL7a, RPS18, RPSa), act on the regulation of translation (eIF4B) or transcription (HSF1, IRF6, MYC, TSC22d3). Others act as chaperones (BAG3, HSPA8, HSP90ab1) or in other metabolic or signals transducing processes. Conclusions: We conclude that a moderate reorganisation of the functionality of the ribosomal machinery and translational activity are very important steps for growth and gene expression control in the initial phase of adipogenesis."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1186/1471-2199-13-9"],["dc.identifier.isi","000303817800001"],["dc.identifier.pmid","22436005"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7594"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26895"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2199"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Novel polysome messages and changes in translational activity appear after induction of adipogenesis in 3T3-L1 cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","86"],["dc.bibliographiccitation.journal","Nutrition & Metabolism"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Fromm-Dornieden, Carolin"],["dc.contributor.author","Lytovchenko, Oleksandr"],["dc.contributor.author","Heyde, Silvia von der"],["dc.contributor.author","Behnke, Nina"],["dc.contributor.author","Hogl, Sebastian"],["dc.contributor.author","Berghoff, Janina"],["dc.contributor.author","Koepper, Frederik"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Renne, Ulla"],["dc.contributor.author","Hoeflich, Andreas"],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Brenig, Bertram B."],["dc.contributor.author","Baumgartner, Bernhard G."],["dc.date.accessioned","2018-11-07T09:05:46Z"],["dc.date.available","2018-11-07T09:05:46Z"],["dc.date.issued","2012"],["dc.description.abstract","Background: DOR/TP53INP2 acts both at the chromosomal level as a nuclear co-factor e.g. for the thyroid hormone receptor and at the extrachromosomal level as an organizing factor of the autophagosome. In a previous study, DOR was shown to be down-regulated in skeletal muscle of obese diabetic Zucker fa/fa rats. Methods: To identify sites of differential DOR expression in metabolically active tissues, we measured differences in DOR expression in white adipose tissue (WAT), brown adipose tissue (BAT), skeletal muscle (SM) and heart muscle (HM) by qPCR. To assess whether DOR expression is influenced in the short term by nutritional factors, NMRI mice were fed different fat rich diets (fat diet, FD: 18% or high fat diet, HFD: 80% fat) for one week and DOR expression was compared to NMRI mice fed a control diet (normal diet, ND: 3.3% fat). Additionally, DOR expression was measured in young (45 days old) and adult (100 days old) genetically obese (DU6/DU6i) mice and compared to control (DUKs/DUKsi) animals. Results: ANOVA results demonstrate a significant influence of diet, tissue type and sex on DOR expression in adipose and muscle tissues of FD and HFD mice. In SM, DOR expression was higher in HFD than in FD male mice. In WAT, DOR expression was increased compared to BAT in male FD and HFD mice. In contrast, expression levels in female mice were higher in BAT for both dietary conditions. DOR expression levels in all tissues of 100 days old genetically obese animals were mainly influenced by sex. In HM, DOR expression was higher in male than female animals. Conclusions: DOR expression varies under the influence of dietary fat content, tissue type and sex. We identified target tissues for further studies to analyze the specific function of DOR in obesity. DOR might be part of a defense mechanism against fat storage in high fat diets or obesity."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2012"],["dc.identifier.doi","10.1186/1743-7075-9-86"],["dc.identifier.isi","000311434000001"],["dc.identifier.pmid","22995226"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/8284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25404"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1743-7075"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Extrinsic and intrinsic regulation of DOR/TP53INP2 expression in mice: effects of dietary fat content, tissue type and sex in adipose and muscle tissues"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Wiener klinische Wochenschrift"],["dc.bibliographiccitation.volume","123"],["dc.contributor.author","Fromm-Dornieden, Carolin"],["dc.contributor.author","Lytovchenko, Oleksandr"],["dc.contributor.author","Salinas-Riester, Gabriela"],["dc.contributor.author","Heyde, Silvia von der"],["dc.contributor.author","Beißbarth, Tim"],["dc.contributor.author","Brenig, Bertram B."],["dc.contributor.author","Baumgartner, Bernhard G."],["dc.date.accessioned","2018-11-07T08:50:04Z"],["dc.date.available","2018-11-07T08:50:04Z"],["dc.date.issued","2011"],["dc.identifier.isi","000298356200044"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21605"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.publisher.place","Wien"],["dc.title","Management of early Adipogenesis through Translational Control"],["dc.type","conference_abstract"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]