Abd El Wahed, Ahmed

[["dc.bibliographiccitation.artnumber","e0129682"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Matangkasombut, Ponpan"],["dc.contributor.author","Manopwisedjaroen, Khajohnpong"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:55:51Z"],["dc.date.available","2018-11-07T09:55:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3'non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magneticbead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38 degrees C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100% (n=41), respectively. Conclusions/Significance During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations."],["dc.identifier.doi","10.1371/journal.pone.0129682"],["dc.identifier.isi","000356329900091"],["dc.identifier.pmid","26075598"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36842"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","3648"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Hotop, Sven-Kevin"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Ndiaye, Oumar"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Pessôa, Rodrigo"],["dc.contributor.author","Hufert, Frank"],["dc.contributor.author","Stahl-Hennig, Christiane"],["dc.contributor.author","Frank, Ronald"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Schmidt-Chanasit, Jonas"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Niedrig, Matthias"],["dc.contributor.author","Brönstrup, Mark"],["dc.contributor.author","Fritz, Hans-Joachim"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:50:13Z"],["dc.date.available","2019-07-09T11:50:13Z"],["dc.date.issued","2019"],["dc.description.abstract","Zika virus (ZIKV) is a mosquito-borne flavivirus. Homologous proteins of different flaviviruses display high degrees of sequence identity, especially within subgroups. This leads to extensive immunological cross-reactivity and corresponding problems for developing a ZIKV-specific serological assay. In this study, peptide microarrays were employed to identify individual ZIKV antibody targets with promise in differential diagnosis. A total of 1643 overlapping oligopeptides were synthesized and printed onto glass slides. Together, they encompass the full amino acid sequences of ZIKV proteomes of African, Brazilian, USA, and French Polynesian origins. The resulting ZIKV scanning microarray chips were used to screen three pools of sera from recent Zika outbreaks in Senegal and Cape Verde, in Brazil, and from overseas travelers returning to the EU. Together with a mixed pool of well characterized, archived sera of patients suffering from infections by dengue, yellow fever, tick-borne encephalitis, and West Nile viruses, a total of 42 sera went into the study. Sixty-eight antibody target regions were identified. Most of which were hitherto unknown. Alignments and sequence comparisons revealed 13 of which could be classified as bona fide ZIKV-specific. These identified antibody target regions constitute a founding set of analytical tools for serological discrimination of ZIKV from other flaviviruses."],["dc.identifier.doi","10.1038/s41598-019-40224-2"],["dc.identifier.pmid","30842564"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15882"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59723"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2045-2322"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","630"],["dc.title","Diagnosing Zika virus infection against a background of other flaviviruses: Studies in high resolution serological analysis."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0004953"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS Neglected Tropical Diseases"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Prueger, Pauline"],["dc.contributor.author","Kaiser, Marco"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Ubol, Sukathida"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Leparc-Goffart, Isabelle"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Niedrig, Matthias"],["dc.date.accessioned","2018-11-07T10:09:05Z"],["dc.date.available","2018-11-07T10:09:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Background Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. Methodology/Principal Findings In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%. Conclusions/Significance The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need."],["dc.identifier.doi","10.1371/journal.pntd.0004953"],["dc.identifier.isi","000385627900024"],["dc.identifier.pmid","27685649"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13760"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39593"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1935-2735"],["dc.rights.access","openAccess"],["dc.title","A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]