Abd El Wahed, Ahmed

[["dc.bibliographiccitation.artnumber","e71642"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","El-Deeb, Ayman"],["dc.contributor.author","El-Tholoth, Mohamed"],["dc.contributor.author","Abd El Kader, Hanaa"],["dc.contributor.author","Ahmed, Abeer"],["dc.contributor.author","Hassan, Sayed"],["dc.contributor.author","Hoffmann, Bernd"],["dc.contributor.author","Haas, Bernd"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:21:11Z"],["dc.date.available","2018-11-07T09:21:11Z"],["dc.date.issued","2013"],["dc.description.abstract","Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection."],["dc.identifier.doi","10.1371/journal.pone.0071642"],["dc.identifier.isi","000324527300028"],["dc.identifier.pmid","23977101"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29055"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","A Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","134"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Veterinary Sciences"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Yehia, Nahed"],["dc.contributor.author","Eldemery, Fatma"],["dc.contributor.author","Arafa, Abdel-Satar"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","El Sanousi, Ahmed"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Shalaby, Mohamed"],["dc.date.accessioned","2021-08-12T07:46:06Z"],["dc.date.available","2021-08-12T07:46:06Z"],["dc.date.issued","2021"],["dc.description.abstract","The H9N2 subtype of avian influenza A virus (aIAV) is circulating among birds worldwide, leading to severe economic losses. H9N2 cocirculation with other highly pathogenic aIAVs has the potential to contribute to the rise of new strains with pandemic potential. Therefore, rapid detection of H9 aIAVs infection is crucial to control virus spread. A qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of aIAV subtype H9N2 was developed. All results were compared to the gold standard (real-time reverse transcription polymerase chain reaction (RT-PCR)). The RT-RPA assay was designed to detect the hemagglutinin (HA) gene of H9N2 by testing three pairs of primers and a probe. A serial concentration between 106 and 100 EID50 (50% embryo infective dose)/mL was applied to calculate the analytical sensitivity. The H9 RT-RPA assay was highly sensitive as the lowest concentration point of a standard range at one EID50/mL was detected after 5 to 8 min. The H9N2 RT-RPA assay was highly specific as nucleic acid extracted from H9 negative samples and from other avian pathogens were not cross detected. The diagnostic sensitivity when testing clinical samples was 100% for RT-RPA and RT-PCR. In conclusion, H9N2 RT-RPA is a rapid sensitive and specific assay that easily operable in a portable device for field diagnosis of aIAV H9N2."],["dc.description.abstract","The H9N2 subtype of avian influenza A virus (aIAV) is circulating among birds worldwide, leading to severe economic losses. H9N2 cocirculation with other highly pathogenic aIAVs has the potential to contribute to the rise of new strains with pandemic potential. Therefore, rapid detection of H9 aIAVs infection is crucial to control virus spread. A qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of aIAV subtype H9N2 was developed. All results were compared to the gold standard (real-time reverse transcription polymerase chain reaction (RT-PCR)). The RT-RPA assay was designed to detect the hemagglutinin (HA) gene of H9N2 by testing three pairs of primers and a probe. A serial concentration between 106 and 100 EID50 (50% embryo infective dose)/mL was applied to calculate the analytical sensitivity. The H9 RT-RPA assay was highly sensitive as the lowest concentration point of a standard range at one EID50/mL was detected after 5 to 8 min. The H9N2 RT-RPA assay was highly specific as nucleic acid extracted from H9 negative samples and from other avian pathogens were not cross detected. The diagnostic sensitivity when testing clinical samples was 100% for RT-RPA and RT-PCR. In conclusion, H9N2 RT-RPA is a rapid sensitive and specific assay that easily operable in a portable device for field diagnosis of aIAV H9N2."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/vetsci8070134"],["dc.identifier.pii","vetsci8070134"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88619"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","2306-7381"],["dc.relation.orgunit","Abteilung Mikrobiologie und Tierhygiene"],["dc.rights","CC BY 4.0"],["dc.title","Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Avian Influenza Virus H9N2 HA Gene"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","PLoS currents"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2019-07-09T11:40:12Z"],["dc.date.available","2019-07-09T11:40:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranean and imported cases to Europe has alerted public health authorities. Currently, detection of MERS-CoV in patient samples is done by real-time RT-PCR. Samples collected from suspected cases are sent to highly-equipped centralized laboratories for screening. A rapid point-of-care test is needed to allow more widespread mobile detection of the virus directly from patient material. In this study, we describe the development of a reverse transcription isothermal Recombinase Polymerase Amplification (RT-RPA) assay for the identification of MERS-CoV. A partial nucleocapsid gene RNA molecular standard of MERS-coronavirus was used to determine the assay sensitivity. The isothermal (42°C) MERS-CoV RT-RPA was as sensitive as real-time RT-PCR (10 RNA molecules), rapid (3-7 minutes) and mobile (using tubescanner weighing 1kg). The MERS-CoV RT-RPA showed cross-detection neither of any of the RNAs of several coronaviruses and respiratory viruses affecting humans nor of the human genome. The developed isothermal real-time RT-RPA is ideal for rapid mobile molecular MERS-CoV monitoring in acute patients and may also facilitate the search for the animal reservoir of MERS-CoV."],["dc.identifier.doi","10.1371/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364"],["dc.identifier.fs","601231"],["dc.identifier.pmid","24459611"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10752"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58113"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2157-3999"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","244"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Veterinary Research"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","El-Deeb, Ayman"],["dc.contributor.author","El-Tholoth, Mohamed"],["dc.contributor.author","Hoffmann, Donata"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:42:52Z"],["dc.date.available","2019-07-09T11:42:52Z"],["dc.date.issued","2016"],["dc.description.abstract","Abstract Background Lumpy skin disease virus (LSDV) is a Capripoxvirus infecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed. Results The LSDV RPA assay was performed at 42 °C and detected down to 179 DNA copies/reaction in a maximum of 15 min. Unspecific amplification was observed with neither LSDV-negative samples (n = 12) nor nucleic acid preparations from orf virus, bovine papular stomatitis virus, cowpoxvirus, Peste des petits ruminants and Blue tongue virus (serotypes 1, 6 and 8). The clinical sensitivity of the LSDV RPA assay matched 100 % (n = 22) to real-time PCR results. In addition, the LSDV RPA assay detected sheep and goat poxviruses. Conclusion The LSDV RPA assay is a rapid and sensitive test that could be implemented in field or at quarantine stations for the identification of LSDV infected case."],["dc.identifier.doi","10.1186/s12917-016-0875-5"],["dc.identifier.pmid","27806722"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13902"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58768"],["dc.language.iso","en"],["dc.rights.access","openAccess"],["dc.rights.holder","The Author(s)."],["dc.title","Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0129682"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Matangkasombut, Ponpan"],["dc.contributor.author","Manopwisedjaroen, Khajohnpong"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:55:51Z"],["dc.date.available","2018-11-07T09:55:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3'non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magneticbead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38 degrees C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100% (n=41), respectively. Conclusions/Significance During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations."],["dc.identifier.doi","10.1371/journal.pone.0129682"],["dc.identifier.isi","000356329900091"],["dc.identifier.pmid","26075598"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36842"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e0004953"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","PLoS Neglected Tropical Diseases"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Prueger, Pauline"],["dc.contributor.author","Kaiser, Marco"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Ubol, Sukathida"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Leparc-Goffart, Isabelle"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Niedrig, Matthias"],["dc.date.accessioned","2018-11-07T10:09:05Z"],["dc.date.available","2018-11-07T10:09:05Z"],["dc.date.issued","2016"],["dc.description.abstract","Background Chikungunya virus (CHIKV) is a mosquito-borne virus currently transmitted in about 60 countries. CHIKV causes acute flu-like symptoms and in many cases prolonged musculoskeletal and joint pain. Detection of the infection is mostly done using RT-RCR or ELISA, which are not suitable for point-of-care diagnosis. Methodology/Principal Findings In this study, a reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of the CHIKV was developed. The assay sensitivity, specificity, and cross-reactivity were tested. CHIKV RT-RPA assay detected down to 80 genome copies/reaction in a maximum of 15 minutes. It successfully identified 18 isolates representing the three CHIKV genotypes. No cross-reactivity was detected to other alphaviruses and arboviruses except O'nyong'nyong virus, which could be differentiated by a modified RPA primer pair. Seventy-eight samples were screened both by RT-RPA and real-time RT-PCR. The diagnostic sensitivity and specificity of the CHIKV RT-RPA assay were determined at 100%. Conclusions/Significance The developed RT-RPA assay represents a promising method for the molecular detection of CHIKV at point of need."],["dc.identifier.doi","10.1371/journal.pntd.0004953"],["dc.identifier.isi","000385627900024"],["dc.identifier.pmid","27685649"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13760"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39593"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1935-2735"],["dc.rights.access","openAccess"],["dc.title","A Field-Deployable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of the Chikungunya Virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]