Now showing 1 - 10 of 19
  • 2013Journal Article
    [["dc.bibliographiccitation.artnumber","e71642"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","El-Deeb, Ayman"],["dc.contributor.author","El-Tholoth, Mohamed"],["dc.contributor.author","Abd El Kader, Hanaa"],["dc.contributor.author","Ahmed, Abeer"],["dc.contributor.author","Hassan, Sayed"],["dc.contributor.author","Hoffmann, Bernd"],["dc.contributor.author","Haas, Bernd"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:21:11Z"],["dc.date.available","2018-11-07T09:21:11Z"],["dc.date.issued","2013"],["dc.description.abstract","Foot-and-mouth disease (FMD) is a trans-boundary viral disease of livestock, which causes huge economic losses and constitutes a serious infectious threat for livestock farming worldwide. Early diagnosis of FMD helps to diminish its impact by adequate outbreak management. In this study, we describe the development of a real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of FMD virus (FMDV). The FMDV RT-RPA design targeted the 3D gene of FMDV and a 260 nt molecular RNA standard was used for assay validation. The RT-RPA assay was fast (4-10 minutes) and the analytical sensitivity was determined at 1436 RNA molecules detected by probit regression analysis. The FMDV RT-RPA assay detected RNA prepared from all seven FMDV serotypes but did not detect classical swine fever virus or swine vesicular disease virus. The FMDV RT-RPA assay was used in the field during the recent FMD outbreak in Egypt. In clinical samples, reverse transcription polymerase chain reaction (RT-PCR) and RT-RPA showed a diagnostic sensitivity of 100% and 98%, respectively. In conclusion, FMDV RT-RPA was quicker and much easier to handle in the field than real-time RT-PCR. Thus RT-RPA could be easily implemented to perform diagnostics at quarantine stations or farms for rapid spot-of-infection detection."],["dc.identifier.doi","10.1371/journal.pone.0071642"],["dc.identifier.isi","000324527300028"],["dc.identifier.pmid","23977101"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10753"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29055"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","A Portable Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Foot-and-Mouth Disease Virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
    Details DOI PMID PMC WOS
  • 2021Journal Article Research Paper
    [["dc.bibliographiccitation.firstpage","134"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Veterinary Sciences"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Yehia, Nahed"],["dc.contributor.author","Eldemery, Fatma"],["dc.contributor.author","Arafa, Abdel-Satar"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","El Sanousi, Ahmed"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Shalaby, Mohamed"],["dc.date.accessioned","2021-08-12T07:46:06Z"],["dc.date.available","2021-08-12T07:46:06Z"],["dc.date.issued","2021"],["dc.description.abstract","The H9N2 subtype of avian influenza A virus (aIAV) is circulating among birds worldwide, leading to severe economic losses. H9N2 cocirculation with other highly pathogenic aIAVs has the potential to contribute to the rise of new strains with pandemic potential. Therefore, rapid detection of H9 aIAVs infection is crucial to control virus spread. A qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of aIAV subtype H9N2 was developed. All results were compared to the gold standard (real-time reverse transcription polymerase chain reaction (RT-PCR)). The RT-RPA assay was designed to detect the hemagglutinin (HA) gene of H9N2 by testing three pairs of primers and a probe. A serial concentration between 106 and 100 EID50 (50% embryo infective dose)/mL was applied to calculate the analytical sensitivity. The H9 RT-RPA assay was highly sensitive as the lowest concentration point of a standard range at one EID50/mL was detected after 5 to 8 min. The H9N2 RT-RPA assay was highly specific as nucleic acid extracted from H9 negative samples and from other avian pathogens were not cross detected. The diagnostic sensitivity when testing clinical samples was 100% for RT-RPA and RT-PCR. In conclusion, H9N2 RT-RPA is a rapid sensitive and specific assay that easily operable in a portable device for field diagnosis of aIAV H9N2."],["dc.description.abstract","The H9N2 subtype of avian influenza A virus (aIAV) is circulating among birds worldwide, leading to severe economic losses. H9N2 cocirculation with other highly pathogenic aIAVs has the potential to contribute to the rise of new strains with pandemic potential. Therefore, rapid detection of H9 aIAVs infection is crucial to control virus spread. A qualitative reverse transcription recombinase polymerase amplification (RT-RPA) assay for the detection of aIAV subtype H9N2 was developed. All results were compared to the gold standard (real-time reverse transcription polymerase chain reaction (RT-PCR)). The RT-RPA assay was designed to detect the hemagglutinin (HA) gene of H9N2 by testing three pairs of primers and a probe. A serial concentration between 106 and 100 EID50 (50% embryo infective dose)/mL was applied to calculate the analytical sensitivity. The H9 RT-RPA assay was highly sensitive as the lowest concentration point of a standard range at one EID50/mL was detected after 5 to 8 min. The H9N2 RT-RPA assay was highly specific as nucleic acid extracted from H9 negative samples and from other avian pathogens were not cross detected. The diagnostic sensitivity when testing clinical samples was 100% for RT-RPA and RT-PCR. In conclusion, H9N2 RT-RPA is a rapid sensitive and specific assay that easily operable in a portable device for field diagnosis of aIAV H9N2."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/vetsci8070134"],["dc.identifier.pii","vetsci8070134"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/88619"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-448"],["dc.relation.eissn","2306-7381"],["dc.relation.orgunit","Abteilung Mikrobiologie und Tierhygiene"],["dc.rights","CC BY 4.0"],["dc.title","Reverse Transcription Recombinase Polymerase Amplification Assay for Rapid Detection of Avian Influenza Virus H9N2 HA Gene"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
    Details DOI
  • 2013Journal Article
    [["dc.bibliographiccitation.journal","PLoS currents"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2019-07-09T11:40:12Z"],["dc.date.available","2019-07-09T11:40:12Z"],["dc.date.issued","2013"],["dc.description.abstract","The emergence of Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in the eastern Mediterranean and imported cases to Europe has alerted public health authorities. Currently, detection of MERS-CoV in patient samples is done by real-time RT-PCR. Samples collected from suspected cases are sent to highly-equipped centralized laboratories for screening. A rapid point-of-care test is needed to allow more widespread mobile detection of the virus directly from patient material. In this study, we describe the development of a reverse transcription isothermal Recombinase Polymerase Amplification (RT-RPA) assay for the identification of MERS-CoV. A partial nucleocapsid gene RNA molecular standard of MERS-coronavirus was used to determine the assay sensitivity. The isothermal (42°C) MERS-CoV RT-RPA was as sensitive as real-time RT-PCR (10 RNA molecules), rapid (3-7 minutes) and mobile (using tubescanner weighing 1kg). The MERS-CoV RT-RPA showed cross-detection neither of any of the RNAs of several coronaviruses and respiratory viruses affecting humans nor of the human genome. The developed isothermal real-time RT-RPA is ideal for rapid mobile molecular MERS-CoV monitoring in acute patients and may also facilitate the search for the animal reservoir of MERS-CoV."],["dc.identifier.doi","10.1371/currents.outbreaks.62df1c7c75ffc96cd59034531e2e8364"],["dc.identifier.fs","601231"],["dc.identifier.pmid","24459611"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/10752"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58113"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","2157-3999"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Reverse transcription recombinase polymerase amplification assay for the detection of middle East respiratory syndrome coronavirus."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
    Details DOI PMID PMC
  • 2015Journal Article
    [["dc.bibliographiccitation.firstpage","45"],["dc.bibliographiccitation.journal","Journal of Virological Methods"],["dc.bibliographiccitation.lastpage","49"],["dc.bibliographiccitation.volume","223"],["dc.contributor.author","Yehia, Nahed"],["dc.contributor.author","Arafa, Abdel-Satar"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","El-Sanousi, Ahmed A."],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.date.accessioned","2021-06-01T10:49:45Z"],["dc.date.available","2021-06-01T10:49:45Z"],["dc.date.issued","2015"],["dc.identifier.doi","10.1016/j.jviromet.2015.07.011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86399"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.issn","0166-0934"],["dc.title","Development of reverse transcription recombinase polymerase amplification assay for avian influenza H5N1 HA gene detection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
    Details DOI
  • 2013Journal Article
    [["dc.bibliographiccitation.firstpage","337"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Journal of Virological Methods"],["dc.bibliographiccitation.lastpage","340"],["dc.bibliographiccitation.volume","193"],["dc.contributor.author","Amer, H. M."],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","Almajhdi, F. N."],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:18:21Z"],["dc.date.available","2018-11-07T09:18:21Z"],["dc.date.issued","2013"],["dc.description.abstract","Bovine coronavirus (BCoV) is an economically significant cause of calf scours and winter dysentery of adult cattle, and may induce respiratory tract infections in cattle of all ages. Early diagnosis of BCoV helps to diminish its burden on the dairy and beef industry. Real-time RT-PCR assay for the detection of BCoV has been described, but it is relatively expensive, requires well-equipped laboratories and is not suitable for on-site screening. A novel assay, using reverse transcription recombinase polymerase amplification (RT-RPA), for the detection of BCoV is developed. The BCoV RT-RPA was rapid (10-20 min) and has an analytical sensitivity of 19 molecules. No cross-reactivity with other viruses causing bovine gastrointestinal and/or respiratory infections was observed. The assay performance on clinical samples was validated by testing 16 fecal and 14 nasal swab specimens and compared to real-time RT-PCR. Both assays provided comparable results. The RT-RPA assay was significantly more rapid than the real-time RT-PCR assay. The BCoV RT-RPA constitutes a suitable accurate, sensitive and rapid alternative to the common measures used for BCoV diagnosis. In addition, the use of a portable fluorescence reading device extends its application potential to use in the field and point-of-care diagnosis. (C) 2013 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.jviromet.2013.06.027"],["dc.identifier.isi","000325190400013"],["dc.identifier.pmid","23811231"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28393"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0166-0934"],["dc.title","A new approach for diagnosis of bovine coronavirus using a reverse transcription recombinase polymerase amplification assay"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]
    Details DOI PMID PMC WOS
  • 2018Journal Article
    [["dc.bibliographiccitation.firstpage","23"],["dc.bibliographiccitation.journal","Journal of Clinical Virology"],["dc.bibliographiccitation.lastpage","27"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Hansen, Sören"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Sanabani, Sabri S."],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Böhlken-Fascher, Susanne"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Sall, Amadou A."],["dc.contributor.author","Bekaert, Michaël"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2020-12-10T14:25:03Z"],["dc.date.available","2020-12-10T14:25:03Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.jcv.2018.07.001"],["dc.identifier.issn","1386-6532"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72419"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Combination random isothermal amplification and nanopore sequencing for rapid identification of the causative agent of an outbreak"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
    Details DOI
  • 2020Book Chapter
    [["dc.bibliographiccitation.firstpage","165"],["dc.bibliographiccitation.lastpage","179"],["dc.bibliographiccitation.seriesnr","2142"],["dc.contributor.author","Diagne, Cheikh Tidiane"],["dc.contributor.author","Faye, Martin"],["dc.contributor.author","Lopez-Jimena, Benjamin"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Loucoubar, Cheikh"],["dc.contributor.author","Fall, Cheikh"],["dc.contributor.author","Mencatelli, Giulia"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Faye, Ousmane"],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.editor","Kobinger, Gary"],["dc.contributor.editor","Racine, Trina"],["dc.date.accessioned","2021-06-02T10:44:21Z"],["dc.date.available","2021-06-02T10:44:21Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1007/978-1-0716-0581-3_14"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/87006"],["dc.notes.intern","DOI-Import GROB-425"],["dc.publisher","Springer US"],["dc.publisher.place","New York, NY"],["dc.relation.crisseries","Methods in Molecular Biology"],["dc.relation.eisbn","978-1-0716-0581-3"],["dc.relation.isbn","978-1-0716-0580-6"],["dc.relation.ispartof","Methods in Molecular Biology"],["dc.relation.ispartof","Zika Virus : Methods and Protocols"],["dc.relation.ispartofseries","Methods in Molecular Biology; 2142"],["dc.title","Comparative Analysis of Zika Virus Detection by RT-qPCR, RT-LAMP, and RT-RPA"],["dc.type","book_chapter"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
    Details DOI
  • 2019Journal Article
    [["dc.bibliographiccitation.firstpage","56"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Veterinary Sciences"],["dc.bibliographiccitation.volume","6"],["dc.contributor.author","Albayrak, Harun"],["dc.contributor.author","Yazici, Zafer"],["dc.contributor.author","Ozan, Emre"],["dc.contributor.author","Tamer, Cuneyt"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Wehner, Stefanie"],["dc.contributor.author","Ulrich, Kristina"],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2021-06-01T10:48:49Z"],["dc.date.available","2021-06-01T10:48:49Z"],["dc.date.issued","2019"],["dc.description.abstract","A respiratory disease outbreak on a cattle farm in northern Turkey produced respiratory tract symptoms and severe pneumonia symptoms in 20 calves. Eight calves died, and a lung specimen from one carcass was analysed for bacteria and for viruses of the Bovine respiratory diseases complex. Bacteriological analysis was negative, but antigen detection ELISA and RT-PCR results indicated the presence of Bovine parainfluenza virus (BPIV). Virus isolation succeeded on Madin-Darby Bovine Kidney cells, and subsequent whole genome sequencing and phylogenetic analysis identified BPIV-3c. This is the first report of BPIV-3c isolation from cattle in Turkey, indicating the need for more virological and epidemiological studies."],["dc.identifier.doi","10.3390/vetsci6020056"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86065"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation.eissn","2306-7381"],["dc.title","Characterisation of the First Bovine Parainfluenza Virus 3 Isolate Detected in Cattle in Turkey"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
    Details DOI
  • 2016Journal Article
    [["dc.bibliographiccitation.artnumber","244"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Veterinary Research"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Shalaby, Mohamed A."],["dc.contributor.author","El-Deeb, Ayman"],["dc.contributor.author","El-Tholoth, Mohamed"],["dc.contributor.author","Hoffmann, Donata"],["dc.contributor.author","Czerny, Claus-Peter"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.date.accessioned","2019-07-09T11:42:52Z"],["dc.date.available","2019-07-09T11:42:52Z"],["dc.date.issued","2016"],["dc.description.abstract","Abstract Background Lumpy skin disease virus (LSDV) is a Capripoxvirus infecting cattle and Buffalos. Lumpy skin disease (LSD) leads to significant economic losses due to hide damage, reduction of milk production, mastitis, infertility and mortalities (10 %). Early detection of the virus is crucial to start appropriate outbreak control measures. Veterinarians rely on the presence of the characteristic clinical signs of LSD. Laboratory diagnostics including virus isolation, sequencing and real-time polymerase chain reaction (PCR) are performed at well-equipped laboratories. In this study, a portable, simple, and rapid recombinase polymerase amplification (RPA) assay for the detection of LSDV-genome for the use on farms was developed. Results The LSDV RPA assay was performed at 42 °C and detected down to 179 DNA copies/reaction in a maximum of 15 min. Unspecific amplification was observed with neither LSDV-negative samples (n = 12) nor nucleic acid preparations from orf virus, bovine papular stomatitis virus, cowpoxvirus, Peste des petits ruminants and Blue tongue virus (serotypes 1, 6 and 8). The clinical sensitivity of the LSDV RPA assay matched 100 % (n = 22) to real-time PCR results. In addition, the LSDV RPA assay detected sheep and goat poxviruses. Conclusion The LSDV RPA assay is a rapid and sensitive test that could be implemented in field or at quarantine stations for the identification of LSDV infected case."],["dc.identifier.doi","10.1186/s12917-016-0875-5"],["dc.identifier.pmid","27806722"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13902"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58768"],["dc.language.iso","en"],["dc.rights.access","openAccess"],["dc.rights.holder","The Author(s)."],["dc.title","Recombinase polymerase amplification assay for rapid detection of lumpy skin disease virus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]
    Details DOI PMID PMC
  • 2015Journal Article
    [["dc.bibliographiccitation.artnumber","e0129682"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Abd El Wahed, Ahmed"],["dc.contributor.author","Patel, Pranav"],["dc.contributor.author","Faye, Oumar"],["dc.contributor.author","Thaloengsok, Sasikanya"],["dc.contributor.author","Heidenreich, Doris"],["dc.contributor.author","Matangkasombut, Ponpan"],["dc.contributor.author","Manopwisedjaroen, Khajohnpong"],["dc.contributor.author","Sakuntabhai, Anavaj"],["dc.contributor.author","Sall, Amadou Alpha"],["dc.contributor.author","Hufert, Frank T."],["dc.contributor.author","Weidmann, Manfred"],["dc.date.accessioned","2018-11-07T09:55:51Z"],["dc.date.available","2018-11-07T09:55:51Z"],["dc.date.issued","2015"],["dc.description.abstract","Background Over 2.5 billion people are exposed to the risk of contracting dengue fever (DF). Early diagnosis of DF helps to diminish its burden on public health. Real-time reverse transcription polymerase amplification assays (RT-PCR) are the standard method for molecular detection of the dengue virus (DENV). Real-time RT-PCR analysis is not suitable for on-site screening since mobile devices are large, expensive, and complex. In this study, two RT-recombinase polymerase amplification (RT-RPA) assays were developed to detect DENV1-4. Methodology/Principal Findings Using two quantitative RNA molecular standards, the analytical sensitivity of a RT-RPA targeting the 3'non-translated region of DENV1-4 was found to range from 14 (DENV4) to 241 (DENV1-3) RNA molecules detected. The assay was specific and did not cross detect other Flaviviruses. The RT-RPA assay was tested in a mobile laboratory combining magneticbead based total nucleic acid extraction and a portable detection device in Kedougou (Senegal) and in Bangkok (Thailand). In Kedougou, the RT-RPA was operated at an ambient temperature of 38 degrees C with auxiliary electricity tapped from a motor vehicle and yielded a clinical sensitivity and specificity of 98% (n=31) and 100% (n=23), respectively. While in the field trial in Bangkok, the clinical sensitivity and specificity were 72% (n=90) and 100% (n=41), respectively. Conclusions/Significance During the first 5 days of infection, the developed DENV1-4 RT-RPA assays constitute a suitable accurate and rapid assay for DENV diagnosis. Moreover, the use of a portable fluorescence-reading device broadens its application potential to the point-of-care for outbreak investigations."],["dc.identifier.doi","10.1371/journal.pone.0129682"],["dc.identifier.isi","000356329900091"],["dc.identifier.pmid","26075598"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/11961"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/36842"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Public Library Science"],["dc.relation.issn","1932-6203"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Recombinase Polymerase Amplification Assay for Rapid Diagnostics of Dengue Infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]
    Details DOI PMID PMC WOS