Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","4921"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","4926"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Khadjeh, Sara"],["dc.contributor.author","Turetzek, Natascha"],["dc.contributor.author","Pechmann, Matthias"],["dc.contributor.author","Schwager, Evelyn E."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Damen, Wim G. M."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T09:12:10Z"],["dc.date.available","2018-11-07T09:12:10Z"],["dc.date.issued","2012"],["dc.description.abstract","Evolution often results in morphologically similar solutions in different organisms, a phenomenon known as convergence. However, there is little knowledge of the processes that lead to convergence at the genetic level. The genes of the Hox cluster control morphology in animals. They may also be central to the convergence of morphological traits, but whether morphological similarities also require similar changes in Hox gene function is disputed. In arthropods, body subdivision into a region with locomotory appendages (\"thorax\") and a region with reduced appendages (\"abdomen\") has evolved convergently in several groups, e. g., spiders and insects. In insects, legs develop in the expression domain of the Hox gene Antennapedia (Antp), whereas the Hox genes Ultrabithorax (Ubx) and abdominal-A mediate leg repression in the abdomen. Here, we show that, unlike Antp in insects, the Antp gene in the spider Achaearanea tepidariorum represses legs in the first segment of the abdomen (opisthosoma), and that Antp and Ubx are redundant in the following segment. The down-regulation of Antp in A. tepidariorum leads to a striking 10-legged phenotype. We present evidence from ectopic expression of the spider Antp gene in Drosophila embryos and imaginal tissue that this unique function of Antp is not due to changes in the Antp protein, but likely due to divergent evolution of cofactors, Hox collaborators or target genes in spiders and flies. Our results illustrate an interesting example of convergent evolution of abdominal leg repression in arthropods by altering the role of distinct Hox genes at different levels of their action."],["dc.identifier.doi","10.1073/pnas.1116421109"],["dc.identifier.isi","000302164200046"],["dc.identifier.pmid","22421434"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26887"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Divergent role of the Hox gene Antennapedia in spiders is responsible for the convergent evolution of abdominal limb repression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","700"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Evolution & Development"],["dc.bibliographiccitation.lastpage","704"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Schmid, Bernhard G. M."],["dc.contributor.author","Zimowska, Grazyna"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:09:26Z"],["dc.date.available","2018-11-07T11:09:26Z"],["dc.date.issued","2008"],["dc.description.abstract","orthodenticle (otd) genes are found throughout the animal kingdom and encode well-studied homeodomain transcription factors that share conserved functions in cephalization, head segmentation, brain patterning, and the differentiation of photoreceptors. Otd proteins have been proposed as ancestral key players in anterior determination despite a high level of variation in gene expression at early developmental stages: otd is expressed strictly zygotically in the dipteran Drosophila melanogaster, while otd1 mRNA is contributed maternally to the embryo in the coleopteran Tribolium castaneum and maternal otd1 mRNA is localized to the anterior and posterior pole of the oocyte in the hymopteran Nasonia vitripennis. Here we demonstrate that such changes in otd mRNA expression and localization do not need to represent large phylogenetic distances but can occur even within closely related taxa. We show maternal otd expression in the medfly Ceratitis capitata and maternally localized otd mRNA in the caribfly Anastrepha suspensa, two cyclorrhaphan species closely related to Drosophila. This indicates considerable plasticity in expression and mRNA localization of key developmental genes even within short evolutionary distances."],["dc.identifier.isi","000260499000005"],["dc.identifier.pmid","19021740"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53006"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1520-541X"],["dc.title","Plasticity in mRNA expression and localization of orthodenticle within higher Diptera"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","468"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Applied Entomology"],["dc.bibliographiccitation.lastpage","473"],["dc.bibliographiccitation.volume","138"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Curril, Ingrid M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:38:30Z"],["dc.date.available","2018-11-07T09:38:30Z"],["dc.date.issued","2014"],["dc.description.abstract","Molecular technologies have enabled the generation of various transgenic insect strains for the area-wide control of agricultural pests and vectors of human diseases. Individual maintenance of several diverse transgenic lines or strains involves a lot of resources, and sometimes problems arise that threaten the strains being maintained. Here, we present a way to cost-effectively maintain transgenic lines or strains of the Mediterranean fruit fly Ceratitis capitata by extending their generation time. Immature stages were kept at 20 degrees C instead of 28 degrees C, and the subsequent generation of transgenic flies kept at different temperatures were found to have laboratory fecundity comparable to the untreated transgenic flies. Extension of generation time offers inexpensive strain maintenance as it reduces the resources, time and energy spent on maintenance of transgenic medfly strains, in addition to minimizing exposure of strains to problems sometimes associated with strain maintenance."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1111/jen.12040"],["dc.identifier.isi","000337695900011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33077"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1439-0418"],["dc.relation.issn","0931-2048"],["dc.title","Generation time extension for cost-effective strain maintenance of transgenic Mediterranean fruit fly, Ceratitis capitata [Diptera: Tephritidae]"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1502"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biomolecules"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Montino, Alice"],["dc.contributor.author","Balakrishnan, Karthi"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Trebels, Björn"],["dc.contributor.author","Neumann, Piotr"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2021-12-01T09:22:46Z"],["dc.date.available","2021-12-01T09:22:46Z"],["dc.date.issued","2021"],["dc.description.abstract","Olfaction is crucial for insects to find food sources, mates, and oviposition sites. One of the initial steps in olfaction is facilitated by odorant-binding proteins (OBPs) that translocate hydrophobic odorants through the aqueous olfactory sensilla lymph to the odorant receptor complexes embedded in the dendritic membrane of olfactory sensory neurons. The Tribolium castaneum (Coleoptera, Tenebrionidae) OBPs encoded by the gene pair TcasOBP9A and TcasOBP9B represent the closest homologs to the well-studied Drosophila melanogaster OBP Lush (DmelOBP76a), which mediates pheromone reception. By an electroantennographic analysis, we can show that these two OBPs are not pheromone-specific but rather enhance the detection of a broad spectrum of organic volatiles. Both OBPs are expressed in the antenna but in a mutually exclusive pattern, despite their homology and gene pair character by chromosomal location. A phylogenetic analysis indicates that this gene pair arose at the base of the Cucujiformia, which dates the gene duplication event to about 200 Mio years ago. Therefore, this gene pair is not the result of a recent gene duplication event and the high sequence conservation in spite of their expression in different sensilla is potentially the result of a common function as co-OBPs."],["dc.description.abstract","Olfaction is crucial for insects to find food sources, mates, and oviposition sites. One of the initial steps in olfaction is facilitated by odorant-binding proteins (OBPs) that translocate hydrophobic odorants through the aqueous olfactory sensilla lymph to the odorant receptor complexes embedded in the dendritic membrane of olfactory sensory neurons. The Tribolium castaneum (Coleoptera, Tenebrionidae) OBPs encoded by the gene pair TcasOBP9A and TcasOBP9B represent the closest homologs to the well-studied Drosophila melanogaster OBP Lush (DmelOBP76a), which mediates pheromone reception. By an electroantennographic analysis, we can show that these two OBPs are not pheromone-specific but rather enhance the detection of a broad spectrum of organic volatiles. Both OBPs are expressed in the antenna but in a mutually exclusive pattern, despite their homology and gene pair character by chromosomal location. A phylogenetic analysis indicates that this gene pair arose at the base of the Cucujiformia, which dates the gene duplication event to about 200 Mio years ago. Therefore, this gene pair is not the result of a recent gene duplication event and the high sequence conservation in spite of their expression in different sensilla is potentially the result of a common function as co-OBPs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/biom11101502"],["dc.identifier.pii","biom11101502"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94479"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","2218-273X"],["dc.relation.orgunit","Johann-Friedrich-Blumenbach-Institut für Zoologie und Anthropologie"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Mutually Exclusive Expression of Closely Related Odorant-Binding Proteins 9A and 9B in the Antenna of the Red Flour Beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","341"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","350"],["dc.bibliographiccitation.volume","223"],["dc.contributor.author","Schaeper, Nina D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T09:18:17Z"],["dc.date.available","2018-11-07T09:18:17Z"],["dc.date.issued","2013"],["dc.description.abstract","Arthropod appendages are among the most diverse animal organs and have been adapted to a variety of functions. Due to this diversity, it can be difficult to recognize homologous parts in different appendage types and different species. Gene expression patterns of appendage development genes have been used to overcome this problem and to identify homologous limb portions across different species and their appendages. However, regarding the largest arthropod group, the hexapods, most of these studies focused on members of the winged insects (Pterygota), but primitively wingless groups like the springtails (Collembola) or silverfish and allies (Zygentoma) are underrepresented. We have studied the expression of a set of appendage patterning genes in the firebrat Thermobia domestica and the white springtail Folsomia candida. The expressions of Distal-less (Dll) and dachshund (dac) are generally similar to the patterns reported for pterygote insects. Modifications of gene regulation, for example, the lack of Dll expression in the palp of F. candida mouthparts, however, point to changes in gene function that can make the use of single genes and specific expression domains problematic for homology inference. Such hypotheses should therefore not rely on a small number of genes and should ideally also include information about gene function. The expression patterns of homothorax (hth) and extradenticle (exd) in both species are similar to the patterns of crustaceans and pterygote insects, but differ from those in chelicerates and myriapods. The proximal specificity of hth thus appears to trace from a common hexapod ancestor and also provides a link to the regulation of this gene in crustaceans."],["dc.identifier.doi","10.1007/s00427-013-0449-5"],["dc.identifier.isi","000325810600001"],["dc.identifier.pmid","23873479"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28374"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-041X"],["dc.relation.issn","0949-944X"],["dc.title","Appendage patterning in the primitively wingless hexapods Thermobia domestica (Zygentoma: Lepismatidae) and Folsomia candida (Collembola: Isotomidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","183"],["dc.bibliographiccitation.issue","5-6"],["dc.bibliographiccitation.journal","Gene Expression Patterns"],["dc.bibliographiccitation.lastpage","188"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:24:00Z"],["dc.date.available","2018-11-07T09:24:00Z"],["dc.date.issued","2013"],["dc.description.abstract","The gene nanos (nos) is a maternal-effect gene that plays an important role in posterior patterning and germ cell development in early stage embryos. nos is known from several diverse insect species, but has so far not been described for any Tephritid fruit fly. Here, we report the molecular cloning and expression pattern of the nos orthologous gene, Ccnos, in the Mediterranean fruit fly Ceratitis capitata, which is a destructive pest of high agricultural importance. CcNOS contains 398 amino acids and has a C-terminal region with two conserved CCHC zinc-binding motifs known to be essential for NOS function. Transcripts of Ccnos were confirmed by in situ hybridization to be maternally-derived and localized to the posterior pole of early stage embryos. Regulatory regions of nos have been employed in genetic engineering in some dipterans such as Drosophila and mosquitoes. Given the similarity in spatial and temporal expression between Ccnos and nos orthologs from other dipterans, its regulatory regions will be valuable to generate additional genetic tools that can be applied for engineering purposes to improve the fight against this devastating pest. (C) 2013 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1016/j.gep.2013.03.002"],["dc.identifier.isi","000320093300007"],["dc.identifier.pmid","23567755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29719"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1567-133X"],["dc.title","Molecular cloning and expression of nanos in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","432"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.bibliographiccitation.lastpage","433"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:13:00Z"],["dc.date.available","2018-11-07T11:13:00Z"],["dc.date.issued","2005"],["dc.description.abstract","Genetic engineering promises to improve a technique for controlling medflies that avoids chemical insecticides."],["dc.identifier.doi","10.1038/nbt0405-432"],["dc.identifier.isi","000228197300019"],["dc.identifier.pmid","15815668"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53793"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1087-0156"],["dc.title","Eco-friendly insect management"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","53"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","58"],["dc.bibliographiccitation.volume","219"],["dc.contributor.author","Toegel, Jane Patricia"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T08:34:23Z"],["dc.date.available","2018-11-07T08:34:23Z"],["dc.date.issued","2009"],["dc.description.abstract","The Drosophila spineless (ss) gene is regulated downstream of the appendage gene Distal-less (Dll) and is involved in leg and antenna development. Specifically, loss of ss leads to the homeotic transformation of the arista, the distalmost antennal segment, into tarsal identity, and the loss or fusion of distal leg segments. Here we show that the ss homolog from the red flour beetle Tribolium castaneum also homeotically transforms the beetle antenna into leg, but the extent of the transformation is significantly larger than in Drosophila, as the entire antenna (except for the basal antennifer) is transformed into pretarsal, tibiotarsal, and femoral identity; i.e., the transformation comprises the Dll positive area in both appendages. We interpret the antennal phenotype in Tribolium as evidence for a more exclusive role of ss in antennal determination downstream of Dll in the beetle. By contrast, the fact that, in Drosophila ss mutants, only a small portion of the Dll positive area in the antenna is homeotically transformed indicates that Dll uses additional targets to govern the development of the other antennal segments in the fly."],["dc.identifier.doi","10.1007/s00427-008-0265-5"],["dc.identifier.isi","000261787900007"],["dc.identifier.pmid","19030876"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3537"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17796"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0949-944X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Loss of spineless function transforms the Tribolium antenna into a thoracic leg with pretarsal, tibiotarsal, and femoral identity"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S17"],["dc.bibliographiccitation.journal","BMC Genetics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Eckermann, Kolja N."],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Karami Nejad Ranjbar, Mohammad"],["dc.contributor.author","Ahmed, Hassan M."],["dc.contributor.author","Curril, Ingrid M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:31:32Z"],["dc.date.available","2018-11-07T09:31:32Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: The Sterile Insect Technique (SIT) is an accepted species-specific genetic control approach that acts as an insect birth control measure, which can be improved by biotechnological engineering to facilitate its use and widen its applicability. First transgenic insects carrying a single killing system have already been released in small scale trials. However, to evade resistance development to such transgenic approaches, completely independent ways of transgenic killing should be established and combined. Perspective: Most established transgenic sexing and reproductive sterility systems are based on the binary tTA expression system that can be suppressed by adding tetracycline to the food. However, to create 'redundant killing' an additional independent conditional expression system is required. Here we present a perspective on the use of a second food-controllable binary expression system - the inducible Q system -that could be used in combination with site-specific recombinases to generate independent transgenic killing systems. We propose the combination of an already established transgenic embryonic sexing system to meet the SIT requirement of male-only releases based on the repressible tTA system together with a redundant male-specific reproductive sterility system, which is activated by Q-system controlled site-specific recombination and is based on a spermatogenesis-specifically expressed endonuclease acting on several species-specific target sites leading to chromosome shredding. Conclusion: A combination of a completely independent transgenic sexing and a redundant reproductive male sterility system, which do not share any active components and mediate the induced lethality by completely independent processes, would meet the 'redundant killing' criteria for suppression of resistance development and could therefore be employed in large scale long-term suppression programs using biotechnologically enhanced SIT."],["dc.identifier.doi","10.1186/1471-2156-15-S2-S17"],["dc.identifier.isi","000353980100018"],["dc.identifier.pmid","25471733"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13266"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31554"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.haserratum","/handle/2/108535"],["dc.relation.issn","1471-2156"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Perspective on the combined use of an independent transgenic sexing and a multifactorial reproductive sterility system to avoid resistance development against transgenic Sterile Insect Technique approaches"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","580"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","582"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:11:24Z"],["dc.date.available","2018-11-07T11:11:24Z"],["dc.date.issued","2005"],["dc.description.abstract","Targeted genomic insertion wilt improve the qualitative and quantitative functional comparison of similar transgenes and provide suitable integration points for transgenes of applied interest."],["dc.identifier.doi","10.1038/nmeth0805-580"],["dc.identifier.isi","000230884500013"],["dc.identifier.pmid","16094381"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53426"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1548-7091"],["dc.title","Insect transgenesis by site-specific recombination"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]