Wimmer, Ernst A.

[["dc.bibliographiccitation.artnumber","12594"],["dc.bibliographiccitation.firstpage","12594"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","23"],["dc.contributor.affiliation","Isah, Musa Dan’azumi; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Atika, Bibi; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Dippel, Stefan; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Ahmed, Hassan M. M.; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Wimmer, Ernst A.; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.author","Isah, Musa Dan’azumi"],["dc.contributor.author","Atika, Bibi"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Ahmed, Hassan M. M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.editor","Davies, T. G. Emyr"],["dc.date.accessioned","2022-12-01T08:31:41Z"],["dc.date.available","2022-12-01T08:31:41Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:12:16Z"],["dc.description.abstract","Sperm marking provides a key tool for reproductive biology studies, but it also represents a valuable monitoring tool for genetic pest control strategies such as the sterile insect technique. Sperm-marked lines can be generated by introducing transgenes that mediate the expression of fluorescent proteins during spermatogenesis. The homozygous lines established by transgenesis approaches are going through a genetic bottleneck that can lead to reduced fitness. Transgenic SIT approaches have mostly focused on Dipteran and Lepidopteran pests so far. With this study, we provide sperm-marked lines for the Coleopteran pest model organism, the red flour beetle Tribolium castaneum, based on the β2-tubulin promoter/enhancer driving red (DsRed) or green (EGFP) fluorescence. The obtained lines are reasonably competitive and were thus used for our studies on reproductive biology, confirming the phenomenon of ‘last-male sperm precedence’ and that the spermathecae are deployed for long-term sperm storage, enabling the use of sperm from first mating events even after secondary mating events for a long period of time. The homozygosity and competitiveness of the lines will enable future studies to analyze the controlled process of sperm movement into the long-term storage organ as part of a post-mating cryptic female choice mechanism of this extremely promiscuous species."],["dc.description.sponsorship","Evangelisches Studienwerk e.V., Villigst, Germany"],["dc.description.sponsorship","University of Calabar, Nigeria"],["dc.description.sponsorship","Erasmus Mundus Action 2"],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.3390/ijms232012594"],["dc.identifier.pii","ijms232012594"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118236"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.publisher","MDPI"],["dc.relation.eissn","1422-0067"],["dc.rights","Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."],["dc.title","Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]