Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","1502"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Biomolecules"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Montino, Alice"],["dc.contributor.author","Balakrishnan, Karthi"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Trebels, Björn"],["dc.contributor.author","Neumann, Piotr"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2021-12-01T09:22:46Z"],["dc.date.available","2021-12-01T09:22:46Z"],["dc.date.issued","2021"],["dc.description.abstract","Olfaction is crucial for insects to find food sources, mates, and oviposition sites. One of the initial steps in olfaction is facilitated by odorant-binding proteins (OBPs) that translocate hydrophobic odorants through the aqueous olfactory sensilla lymph to the odorant receptor complexes embedded in the dendritic membrane of olfactory sensory neurons. The Tribolium castaneum (Coleoptera, Tenebrionidae) OBPs encoded by the gene pair TcasOBP9A and TcasOBP9B represent the closest homologs to the well-studied Drosophila melanogaster OBP Lush (DmelOBP76a), which mediates pheromone reception. By an electroantennographic analysis, we can show that these two OBPs are not pheromone-specific but rather enhance the detection of a broad spectrum of organic volatiles. Both OBPs are expressed in the antenna but in a mutually exclusive pattern, despite their homology and gene pair character by chromosomal location. A phylogenetic analysis indicates that this gene pair arose at the base of the Cucujiformia, which dates the gene duplication event to about 200 Mio years ago. Therefore, this gene pair is not the result of a recent gene duplication event and the high sequence conservation in spite of their expression in different sensilla is potentially the result of a common function as co-OBPs."],["dc.description.abstract","Olfaction is crucial for insects to find food sources, mates, and oviposition sites. One of the initial steps in olfaction is facilitated by odorant-binding proteins (OBPs) that translocate hydrophobic odorants through the aqueous olfactory sensilla lymph to the odorant receptor complexes embedded in the dendritic membrane of olfactory sensory neurons. The Tribolium castaneum (Coleoptera, Tenebrionidae) OBPs encoded by the gene pair TcasOBP9A and TcasOBP9B represent the closest homologs to the well-studied Drosophila melanogaster OBP Lush (DmelOBP76a), which mediates pheromone reception. By an electroantennographic analysis, we can show that these two OBPs are not pheromone-specific but rather enhance the detection of a broad spectrum of organic volatiles. Both OBPs are expressed in the antenna but in a mutually exclusive pattern, despite their homology and gene pair character by chromosomal location. A phylogenetic analysis indicates that this gene pair arose at the base of the Cucujiformia, which dates the gene duplication event to about 200 Mio years ago. Therefore, this gene pair is not the result of a recent gene duplication event and the high sequence conservation in spite of their expression in different sensilla is potentially the result of a common function as co-OBPs."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.3390/biom11101502"],["dc.identifier.pii","biom11101502"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/94479"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-478"],["dc.relation.eissn","2218-273X"],["dc.relation.orgunit","Johann-Friedrich-Blumenbach-Institut für Zoologie und Anthropologie"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0/"],["dc.title","Mutually Exclusive Expression of Closely Related Odorant-Binding Proteins 9A and 9B in the Antenna of the Red Flour Beetle Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S17"],["dc.bibliographiccitation.journal","BMC Genetics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Eckermann, Kolja N."],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Karami Nejad Ranjbar, Mohammad"],["dc.contributor.author","Ahmed, Hassan M."],["dc.contributor.author","Curril, Ingrid M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:31:32Z"],["dc.date.available","2018-11-07T09:31:32Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: The Sterile Insect Technique (SIT) is an accepted species-specific genetic control approach that acts as an insect birth control measure, which can be improved by biotechnological engineering to facilitate its use and widen its applicability. First transgenic insects carrying a single killing system have already been released in small scale trials. However, to evade resistance development to such transgenic approaches, completely independent ways of transgenic killing should be established and combined. Perspective: Most established transgenic sexing and reproductive sterility systems are based on the binary tTA expression system that can be suppressed by adding tetracycline to the food. However, to create 'redundant killing' an additional independent conditional expression system is required. Here we present a perspective on the use of a second food-controllable binary expression system - the inducible Q system -that could be used in combination with site-specific recombinases to generate independent transgenic killing systems. We propose the combination of an already established transgenic embryonic sexing system to meet the SIT requirement of male-only releases based on the repressible tTA system together with a redundant male-specific reproductive sterility system, which is activated by Q-system controlled site-specific recombination and is based on a spermatogenesis-specifically expressed endonuclease acting on several species-specific target sites leading to chromosome shredding. Conclusion: A combination of a completely independent transgenic sexing and a redundant reproductive male sterility system, which do not share any active components and mediate the induced lethality by completely independent processes, would meet the 'redundant killing' criteria for suppression of resistance development and could therefore be employed in large scale long-term suppression programs using biotechnologically enhanced SIT."],["dc.identifier.doi","10.1186/1471-2156-15-S2-S17"],["dc.identifier.isi","000353980100018"],["dc.identifier.pmid","25471733"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13266"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31554"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.haserratum","/handle/2/108535"],["dc.relation.issn","1471-2156"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Perspective on the combined use of an independent transgenic sexing and a multifactorial reproductive sterility system to avoid resistance development against transgenic Sterile Insect Technique approaches"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","90"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Kollmann, Martin"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Montino, Alice"],["dc.contributor.author","Knoll, Carolin"],["dc.contributor.author","Krala, Milosz"],["dc.contributor.author","Rexer, Karl-Heinz"],["dc.contributor.author","Frank, Sergius"],["dc.contributor.author","Kumpf, Robert"],["dc.contributor.author","Schachtner, Joachim"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T10:06:58Z"],["dc.date.available","2018-11-07T10:06:58Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: The red flour beetle Tribolium castaneum is an emerging insect model organism representing the largest insect order, Coleoptera, which encompasses several serious agricultural and forest pests. Despite the ecological and economic importance of beetles, most insect olfaction studies have so far focused on dipteran, lepidopteran, or hymenopteran systems. Results: Here, we present the first detailed morphological description of a coleopteran olfactory pathway in combination with genome-wide expression analysis of the relevant gene families involved in chemoreception. Our study revealed that besides the antennae, also the mouthparts are highly involved in olfaction and that their respective contribution is processed separately. In this beetle, olfactory sensory neurons from the mouthparts project to the lobus glomerulatus, a structure so far only characterized in hemimetabolous insects, as well as to a so far non-described unpaired glomerularly organized olfactory neuropil in the gnathal ganglion, which we term the gnathal olfactory center. The high number of functional odorant receptor genes expressed in the mouthparts also supports the importance of the maxillary and labial palps in olfaction of this beetle. Moreover, gustatory perception seems equally distributed between antenna and mouthparts, since the number of expressed gustatory receptors is similar for both organs. Conclusions: Our analysis of the T. castaneum chemosensory system confirms that olfactory and gustatory perception are not organotopically separated to the antennae and mouthparts, respectively. The identification of additional olfactory processing centers, the lobus glomerulatus and the gnathal olfactory center, is in contrast to the current picture that in holometabolous insects all olfactory inputs allegedly converge in the antennal lobe. These findings indicate that Holometabola have evolved a wider variety of solutions to chemoreception than previously assumed."],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft [SPP 1392: SCHA 678/13-1, WI 1797/4-1]"],["dc.identifier.doi","10.1186/s12915-016-0304-z"],["dc.identifier.isi","000385883900002"],["dc.identifier.pmid","27751175"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13895"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39197"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1741-7007"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Morphological and Transcriptomic Analysis of a Beetle Chemosensory System Reveals a Gnathal Olfactory Center"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Scientific Reports"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Trebels, Björn"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Schaaf, Magdalina"],["dc.contributor.author","Balakrishnan, Karthi"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Schachtner, Joachim"],["dc.date.accessioned","2021-04-14T08:27:36Z"],["dc.date.available","2021-04-14T08:27:36Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41598-020-57639-x"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82343"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2045-2322"],["dc.title","Adult neurogenesis in the mushroom bodies of red flour beetles (Tribolium castaneum, Herbst) is influenced by the olfactory environment"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","1141"],["dc.bibliographiccitation.journal","BMC Genomics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Kahnt, Joerg"],["dc.contributor.author","Gerischer, Lizzy"],["dc.contributor.author","Opitz, Lennart"],["dc.contributor.author","Schachtner, Joachim"],["dc.contributor.author","Stanke, Mario"],["dc.contributor.author","Schuetz, Stefan"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Angeli, Sergio"],["dc.date.accessioned","2018-11-07T09:31:12Z"],["dc.date.available","2018-11-07T09:31:12Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: Chemoreception is based on the senses of smell and taste that are crucial for animals to find new food sources, shelter, and mates. The initial step in olfaction involves the translocation of odorants from the periphery through the aqueous lymph of the olfactory sensilla to the odorant receptors most likely by chemosensory proteins (CSPs) or odorant binding proteins (OBPs). Results: To better understand the roles of CSPs and OBPs in a coleopteran pest species, the red flour beetle Tribolium castaneum (Coleoptera, Tenebrionidae), we performed transcriptome analyses of male and female antennae, heads, mouthparts, legs, and bodies, which revealed that all 20 CSPs and 49 of the 50 previously annotated OBPs are transcribed. Only six of the 20 CSP are significantly transcriptionally enriched in the main chemosensory tissues (antenna and/or mouthparts), whereas of the OBPs all eight members of the antenna binding proteins II (ABPII) subgroup, 18 of the 20 classic OBP subgroup, the C + OBP, and only five of the 21 C-OBPs show increased chemosensory tissue expression. By MALDI-TOF-TOF MS protein fingerprinting, we confirmed three CSPs, four ABPIIs, three classic OBPs, and four C-OBPs in the antennae. Conclusions: Most of the classic OBPs and all ABPIIs are likely involved in chemoreception. A few are also present in other tissues such as odoriferous glands and testes and may be involved in release or transfer of chemical signals. The majority of the CSPs as well as the C-OBPs are not enriched in antennae or mouthparts, suggesting a more general role in the transport of hydrophobic molecules."],["dc.identifier.doi","10.1186/1471-2164-15-1141"],["dc.identifier.isi","000347728600001"],["dc.identifier.pmid","25523483"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/12946"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31488"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2164"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Tissue-specific transcriptomics, chromosomal localization, and phylogeny of chemosensory and odorant binding proteins from the red flour beetle Tribolium castaneum reveal subgroup specificities for olfaction or more general functions"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","12594"],["dc.bibliographiccitation.firstpage","12594"],["dc.bibliographiccitation.issue","20"],["dc.bibliographiccitation.journal","International Journal of Molecular Sciences"],["dc.bibliographiccitation.volume","23"],["dc.contributor.affiliation","Isah, Musa Dan’azumi; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Atika, Bibi; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Dippel, Stefan; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Ahmed, Hassan M. M.; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.affiliation","Wimmer, Ernst A.; 1Department of Developmental Biology, Johann-Friedrich-Blumenbach-Institute for Zoology and Anthropology, Ernst-Caspari-Haus, GZMB, Georg-August-University Goettingen, Justus-von-Liebig-Weg 11, 37077 Goettingen, Germany"],["dc.contributor.author","Isah, Musa Dan’azumi"],["dc.contributor.author","Atika, Bibi"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Ahmed, Hassan M. M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.editor","Davies, T. G. Emyr"],["dc.date.accessioned","2022-12-01T08:31:41Z"],["dc.date.available","2022-12-01T08:31:41Z"],["dc.date.issued","2022"],["dc.date.updated","2022-11-11T13:12:16Z"],["dc.description.abstract","Sperm marking provides a key tool for reproductive biology studies, but it also represents a valuable monitoring tool for genetic pest control strategies such as the sterile insect technique. Sperm-marked lines can be generated by introducing transgenes that mediate the expression of fluorescent proteins during spermatogenesis. The homozygous lines established by transgenesis approaches are going through a genetic bottleneck that can lead to reduced fitness. Transgenic SIT approaches have mostly focused on Dipteran and Lepidopteran pests so far. With this study, we provide sperm-marked lines for the Coleopteran pest model organism, the red flour beetle Tribolium castaneum, based on the β2-tubulin promoter/enhancer driving red (DsRed) or green (EGFP) fluorescence. The obtained lines are reasonably competitive and were thus used for our studies on reproductive biology, confirming the phenomenon of ‘last-male sperm precedence’ and that the spermathecae are deployed for long-term sperm storage, enabling the use of sperm from first mating events even after secondary mating events for a long period of time. The homozygosity and competitiveness of the lines will enable future studies to analyze the controlled process of sperm movement into the long-term storage organ as part of a post-mating cryptic female choice mechanism of this extremely promiscuous species."],["dc.description.sponsorship","Evangelisches Studienwerk e.V., Villigst, Germany"],["dc.description.sponsorship","University of Calabar, Nigeria"],["dc.description.sponsorship","Erasmus Mundus Action 2"],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.3390/ijms232012594"],["dc.identifier.pii","ijms232012594"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/118236"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-621"],["dc.publisher","MDPI"],["dc.relation.eissn","1422-0067"],["dc.rights","Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/)."],["dc.title","Competitive Sperm-Marked Beetles for Monitoring Approaches in Genetic Biocontrol and Studies in Reproductive Biology"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16"],["dc.bibliographiccitation.journal","Insect Biochemistry and Molecular Biology"],["dc.bibliographiccitation.lastpage","24"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Eckermann, Kolja N."],["dc.contributor.author","Ahmed, Hassan M.M."],["dc.contributor.author","Karami Nejad Ranjbar, Mohammad"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Kitzmann, Peter"],["dc.contributor.author","Isah, Musa D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2020-12-10T14:24:26Z"],["dc.date.available","2020-12-10T14:24:26Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.ibmb.2018.04.001"],["dc.identifier.issn","0965-1748"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72250"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Hyperactive piggyBac transposase improves transformation efficiency in diverse insect species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","32"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","BMC Genomic Data"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Eckermann, Kolja N."],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Carrami, Eli M."],["dc.contributor.author","Ahmed, Hassan M."],["dc.contributor.author","Curril, Ingrid M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2022-06-01T09:39:41Z"],["dc.date.accessioned","2022-08-18T12:35:42Z"],["dc.date.available","2022-06-01T09:39:41Z"],["dc.date.available","2022-08-18T12:35:42Z"],["dc.date.issued","2022-05-04"],["dc.date.updated","2022-07-29T12:07:21Z"],["dc.identifier.citation","BMC Genomic Data. 2022 May 04;23(1):32"],["dc.identifier.doi","10.1186/s12863-022-01051-z"],["dc.identifier.pii","1051"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/108535"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/112943"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-572"],["dc.relation.eissn","2730-6844"],["dc.relation.iserratumof","/handle/2/31554"],["dc.relation.orgunit","Johann-Friedrich-Blumenbach-Institut für Zoologie und Anthropologie"],["dc.relation.orgunit","Abteilung Entwicklungsbiologie"],["dc.rights.holder","The Author(s)"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Correction to: Perspective on the combined use of an independent transgenic sexing and a multifactorial reproductive sterility system to avoid resistance development against transgenic Sterile Insect Technique approaches"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","erratum_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","6189"],["dc.bibliographiccitation.issue","24"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences"],["dc.bibliographiccitation.lastpage","6194"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Karami Nejad Ranjbar, Mohammad"],["dc.contributor.author","Eckermann, Kolja N."],["dc.contributor.author","Ahmed, Hassan M. M."],["dc.contributor.author","Sánchez C., Héctor M."],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Marshall, John M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2020-12-10T18:12:48Z"],["dc.date.available","2020-12-10T18:12:48Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1073/pnas.1713825115"],["dc.identifier.eissn","1091-6490"],["dc.identifier.issn","0027-8424"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/74500"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Consequences of resistance evolution in a Cas9-based sex conversion-suppression gene drive for insect pest management"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]