Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","76"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","New Biotechnology"],["dc.bibliographiccitation.lastpage","84"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Bertin, Sabrina"],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:14:51Z"],["dc.date.available","2018-11-07T11:14:51Z"],["dc.date.issued","2008"],["dc.description.abstract","The Sterile Insect Technique (SIT) involving area-wide release of mass-reared and sterilized pest insects has proven successful to reduce, control and eradicate economically important pest species, such as the Mediterranean fruit fly (medfly). For the efficient application, effective monitoring to assess the number and mating success of the released medflies is essential. Here, we report sperm-specific marking systems based on the spermatogenesis-specific Ceratitis capitata beta 2-tubulin (Cc beta 2t) promoter. Fluorescent sperm can be isolated from testes or spermathecae. The marking does not cause general disadvantages in preliminary laboratory competitiveness assays. Therefore, transgenic sperm marking could serve as a major improvement for monitoring medfly SIT programs. The use of such harmless transgenic markers will serve as an ideal initial condition to transfer insect transgenesis technology from the laboratory to field applications. Moreover, effective and easily recognizable sperm marking will make novel studies possible on medfly reproductive biology which will help to further improve SIT programs."],["dc.identifier.doi","10.1016/j.nbt.2008.02.001"],["dc.identifier.isi","000259343400022"],["dc.identifier.pmid","18504022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54233"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1871-6784"],["dc.title","Fluorescent sperm marking to improve the fight against the pest insect Ceratitis capitata (Wiedemann; Diptera : Tephritidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","820"],["dc.bibliographiccitation.issue","9-10"],["dc.bibliographiccitation.journal","Journal of Applied Entomology"],["dc.bibliographiccitation.lastpage","831"],["dc.bibliographiccitation.volume","132"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Gabrieli, Paolo"],["dc.contributor.author","Siciliano, Paolo"],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Karam, N."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Gasperi, Giuliano"],["dc.date.accessioned","2018-11-07T11:08:36Z"],["dc.date.available","2018-11-07T11:08:36Z"],["dc.date.issued","2008"],["dc.description.abstract","Tephritid fruit fly species cause major economical losses in crops worldwide. Genetic transformation of insect pests, which are targets of the Sterile Insect Technique (SIT), a key component of area-wide pest management, has been achieved for several years. For the medfly Ceratitis capitata as well as several Bactrocera and Anastrepha species, germline transformation can now be used to bioengineer strains that should increase the efficacy and cost-effectiveness of the SIT. Novel transformation vectors, robust genetic markers and diverse promoters to drive stage- and tissue-specific gene expression provide powerful tools to test the contribution that these technologies can make to current SIT programmes."],["dc.identifier.doi","10.1111/j.1439-0418.2008.01347.x"],["dc.identifier.isi","000262106200018"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52820"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1439-0418"],["dc.relation.issn","0931-2048"],["dc.title","Insect transgenesis applied to tephritid pest control"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","18171"],["dc.bibliographiccitation.issue","43"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","18176"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Handler, Alfred M."],["dc.contributor.author","Kittelmann, Sebastian"],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:23:09Z"],["dc.date.available","2018-11-07T11:23:09Z"],["dc.date.issued","2009"],["dc.description.abstract","Insect transgenesis is mainly based on the random genomic integration of DNA fragments embedded into non-autonomous transposable elements. Once a random insertion into a specific location of the genome has been identified as particularly useful with respect to transgene expression, the ability to make the insertion homozygous, and lack of fitness costs, it may be advantageous to use that location for further modification. Here we describe an efficient method for the modification of previously inserted transgenes by the use of the site-specific integration system from phage phiC31 in a tephritid pest species, the Mediterranean fruit fly Ceratitis capitata. First, suitable transgenic strains with randomly integrated attP landing sites within transposon-based vectors were identified by molecular and functional characterization. Second, donor plasmids containing an attB site, with additional markers, and transposon ends were integrated into attP sites by phiC31 integrase-mediated recombination. Third, transposase-encoding 'jumpstarter' strains were created and mated to transgenic strains resulting in the postintegrational excision of transposon ends, which left stably integrated transgene insertions that could not be remobilized. This three-step integration and stabilization system will allow the combination of several transgene-encoded advantageous traits at evaluated genomic positions to generate optimized strains for pest control that minimize environmental concerns."],["dc.identifier.doi","10.1073/pnas.0907264106"],["dc.identifier.isi","000271222500026"],["dc.identifier.pmid","19828439"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56133"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]