Wimmer, Ernst A.

[["dc.bibliographiccitation.artnumber","S10"],["dc.bibliographiccitation.journal","BMC Genetics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Yuval, Boaz"],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Gabrieli, Paolo"],["dc.contributor.author","Bassetti, Federico"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Gasperi, Giuliano"],["dc.date.accessioned","2018-11-07T09:31:33Z"],["dc.date.available","2018-11-07T09:31:33Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: In the Mediterranean fruit fly (medfly), Ceratitis capitata, a highly invasive agricultural pest species, polyandry, associated with sperm precedence, is a recurrent behaviour in the wild. The absence of tools for the unambiguous discrimination between competing sperm from different males in the complex female reproductive tract has strongly limited the understanding of mechanisms controlling sperm dynamics and use. Results: Here we use transgenic medfly lines expressing green or red fluorescent proteins in the spermatozoa, which can be easily observed and unambiguously differentiated within the female fertilization chamber. In twice-mated females, one day after the second mating, sperm from the first male appeared to be homogenously distributed all over the distal portion of each alveolus within the fertilization chamber, whereas sperm from the second male were clearly concentrated in the central portion of each alveolus. This distinct stratified sperm distribution was not maintained over time, as green and red sperm appeared homogeneously mixed seven days after the second mating. This dynamic sperm storage pattern is mirrored by the paternal contribution in the progeny of twice-mated females. Conclusions: Polyandrous medfly females, unlike Drosophila, conserve sperm from two different mates to fertilize their eggs. From an evolutionary point of view, the storage of sperm in a stratified pattern by medfly females may initially favour the fresher ejaculate from the second male. However, as the second male's sperm gradually becomes depleted, the sperm from the first male becomes increasingly available for fertilization. The accumulation of sperm from different males will increase the overall genetic variability of the offspring and will ultimately affect the effective population size. From an applicative point of view, the dynamics of sperm storage and their temporal use by a polyandrous female may have an impact on the Sterile Insect Technique (SIT). Indeed, even if the female's last mate is sterile, an increasing proportion of sperm from a previous mating with a fertile male may contribute to sire viable progeny."],["dc.identifier.doi","10.1186/1471-2156-15-S2-S10"],["dc.identifier.isi","000353980100011"],["dc.identifier.pmid","25470981"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13267"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31555"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2156"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Polyandry in the medfly - shifts in paternity mediated by sperm stratification and mixing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","192"],["dc.bibliographiccitation.journal","Genome Biology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Papanicolaou, Alexie"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Arensburger, Peter"],["dc.contributor.author","Atkinson, Peter W."],["dc.contributor.author","Benoit, Joshua B."],["dc.contributor.author","Bourtzis, Kostas"],["dc.contributor.author","Castanera, Pedro"],["dc.contributor.author","Cavanaugh, John P."],["dc.contributor.author","Chao, Hsu"],["dc.contributor.author","Childers, Christopher"],["dc.contributor.author","Curril, Ingrid"],["dc.contributor.author","Huyen Dinh, Huyen Dinh"],["dc.contributor.author","Doddapaneni, HarshaVardhan"],["dc.contributor.author","Dolan, Amanda"],["dc.contributor.author","Dugan, Shannon"],["dc.contributor.author","Friedrich, Markus"],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Geib, Scott"],["dc.contributor.author","Georgakilas, Georgios"],["dc.contributor.author","Gibbs, Richard A."],["dc.contributor.author","Giers, Sarah D."],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Gonzalez-Guzman, Miguel"],["dc.contributor.author","Guillem-Amat, Ana"],["dc.contributor.author","Han, Y. I."],["dc.contributor.author","Hatzigeorgiou, Artemis G."],["dc.contributor.author","Hernandez-Crespo, Pedro"],["dc.contributor.author","Hughes, Daniel S. T."],["dc.contributor.author","Jones, Jeffery W."],["dc.contributor.author","Karagkouni, Dimitra"],["dc.contributor.author","Koskinioti, Panagiota"],["dc.contributor.author","Lee, Sandra L."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Manni, Mose"],["dc.contributor.author","Mathiopoulos, Kostas"],["dc.contributor.author","Meccariello, Angela"],["dc.contributor.author","Murali, Shwetha C."],["dc.contributor.author","Murphy, Terence D."],["dc.contributor.author","Muzny, Donna M."],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Ortego, Felix"],["dc.contributor.author","Paraskevopoulou, Maria D."],["dc.contributor.author","Poelchau, Monica"],["dc.contributor.author","Qu, Jiaxin"],["dc.contributor.author","Reczko, Martin"],["dc.contributor.author","Robertson, Hugh M."],["dc.contributor.author","Rosendale, Andrew J."],["dc.contributor.author","Rosselot, Andrew E."],["dc.contributor.author","Saccone, Giuseppe"],["dc.contributor.author","Salvemini, Marco"],["dc.contributor.author","Savini, Grazia"],["dc.contributor.author","Schreiner, Patrick"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Siciliano, Paolo"],["dc.contributor.author","Sim, Sheina B."],["dc.contributor.author","Tsiamis, George"],["dc.contributor.author","Urena, Enric"],["dc.contributor.author","Vlachos, Ioannis S."],["dc.contributor.author","Werren, John H."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Worley, Kim C."],["dc.contributor.author","Zacharopoulou, Antigone"],["dc.contributor.author","Richards, Stephen J."],["dc.contributor.author","Handler, Alfred M."],["dc.date.accessioned","2018-11-07T10:08:20Z"],["dc.date.available","2018-11-07T10:08:20Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits a unique ability to invade and adapt to ecological niches throughout tropical and subtropical regions of the world, though medfly infestations have been prevented and controlled by the sterile insect technique (SIT) as part of integrated pest management programs (IPMs). The genetic analysis and manipulation of medfly has been subject to intensive study in an effort to improve SIT efficacy and other aspects of IPM control. Results: The 479 Mb medfly genome is sequenced from adult flies from lines inbred for 20 generations. A high-quality assembly is achieved having a contig N50 of 45.7 kb and scaffold N50 of 4.06 Mb. In-depth curation of more than 1800 messenger RNAs shows specific gene expansions that can be related to invasiveness and host adaptation, including gene families for chemoreception, toxin and insecticide metabolism, cuticle proteins, opsins, and aquaporins. We identify genes relevant to IPM control, including those required to improve SIT. Conclusions: The medfly genome sequence provides critical insights into the biology of one of the most serious and widespread agricultural pests. This knowledge should significantly advance the means of controlling the size and invasive potential of medfly populations. Its close relationship to Drosophila, and other insect species important to agriculture and human health, will further comparative functional and structural studies of insect genomes that should broaden our understanding of gene family evolution."],["dc.identifier.doi","10.1186/s13059-016-1049-2"],["dc.identifier.isi","000384461900002"],["dc.identifier.pmid","27659211"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13887"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39453"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1474-760X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","11"],["dc.bibliographiccitation.journal","Genome Biology"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Papanicolaou, Alexie"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Arensburger, Peter"],["dc.contributor.author","Atkinson, Peter W."],["dc.contributor.author","Benoit, Joshua B."],["dc.contributor.author","Bourtzis, Kostas"],["dc.contributor.author","Castanera, Pedro"],["dc.contributor.author","Cavanaugh, John P."],["dc.contributor.author","Chao, Hsu"],["dc.contributor.author","Childers, Christopher"],["dc.contributor.author","Curril, Ingrid"],["dc.contributor.author","Dinh, Huyen"],["dc.contributor.author","Doddapaneni, HarshaVardhan"],["dc.contributor.author","Dolan, Amanda"],["dc.contributor.author","Dugan, Shannon"],["dc.contributor.author","Friedrich, Markus"],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Geib, Scott"],["dc.contributor.author","Georgakilas, Georgios"],["dc.contributor.author","Gibbs, Richard A."],["dc.contributor.author","Giers, Sarah D."],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Gonzalez-Guzman, Miguel"],["dc.contributor.author","Guillem-Amat, Ana"],["dc.contributor.author","Han, Y. I."],["dc.contributor.author","Hatzigeorgiou, Artemis G."],["dc.contributor.author","Hernandez-Crespo, Pedro"],["dc.contributor.author","Hughes, Daniel S. T."],["dc.contributor.author","Jones, Jeffery W."],["dc.contributor.author","Karagkouni, Dimitra"],["dc.contributor.author","Koskinioti, Panagiota"],["dc.contributor.author","Lee, Sandra L."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Manni, Mose"],["dc.contributor.author","Mathiopoulos, Kostas"],["dc.contributor.author","Meccariello, Angela"],["dc.contributor.author","Munoz-Torres, Monica"],["dc.contributor.author","Murali, Shwetha C."],["dc.contributor.author","Murphy, Terence D."],["dc.contributor.author","Muzny, Donna M."],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Ortego, Felix"],["dc.contributor.author","Paraskevopoulou, Maria D."],["dc.contributor.author","Poelchau, Monica"],["dc.contributor.author","Qu, Jiaxin"],["dc.contributor.author","Reczko, Martin"],["dc.contributor.author","Robertson, Hugh M."],["dc.contributor.author","Rosendale, Andrew J."],["dc.contributor.author","Rosselot, Andrew E."],["dc.contributor.author","Saccone, Giuseppe"],["dc.contributor.author","Salvemini, Marco"],["dc.contributor.author","Savini, Grazia"],["dc.contributor.author","Schreiner, Patrick"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Siciliano, Paolo"],["dc.contributor.author","Sim, Sheina B."],["dc.contributor.author","Tsiamis, George"],["dc.contributor.author","Urena, Enric"],["dc.contributor.author","Vlachos, Ioannis S."],["dc.contributor.author","Werren, John H."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Worley, Kim C."],["dc.contributor.author","Zacharopoulou, Antigone"],["dc.contributor.author","Richards, Stephen J."],["dc.contributor.author","Handler, Alfred M."],["dc.date.accessioned","2018-11-07T10:28:20Z"],["dc.date.available","2018-11-07T10:28:20Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1186/s13059-017-1155-9"],["dc.identifier.isi","000394826300003"],["dc.identifier.pmid","28100280"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14361"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43399"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1474-760X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species (vol 17, 192, 2016)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]