Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","468"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Applied Entomology"],["dc.bibliographiccitation.lastpage","473"],["dc.bibliographiccitation.volume","138"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Curril, Ingrid M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:38:30Z"],["dc.date.available","2018-11-07T09:38:30Z"],["dc.date.issued","2014"],["dc.description.abstract","Molecular technologies have enabled the generation of various transgenic insect strains for the area-wide control of agricultural pests and vectors of human diseases. Individual maintenance of several diverse transgenic lines or strains involves a lot of resources, and sometimes problems arise that threaten the strains being maintained. Here, we present a way to cost-effectively maintain transgenic lines or strains of the Mediterranean fruit fly Ceratitis capitata by extending their generation time. Immature stages were kept at 20 degrees C instead of 28 degrees C, and the subsequent generation of transgenic flies kept at different temperatures were found to have laboratory fecundity comparable to the untreated transgenic flies. Extension of generation time offers inexpensive strain maintenance as it reduces the resources, time and energy spent on maintenance of transgenic medfly strains, in addition to minimizing exposure of strains to problems sometimes associated with strain maintenance."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1111/jen.12040"],["dc.identifier.isi","000337695900011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33077"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1439-0418"],["dc.relation.issn","0931-2048"],["dc.title","Generation time extension for cost-effective strain maintenance of transgenic Mediterranean fruit fly, Ceratitis capitata [Diptera: Tephritidae]"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","183"],["dc.bibliographiccitation.issue","5-6"],["dc.bibliographiccitation.journal","Gene Expression Patterns"],["dc.bibliographiccitation.lastpage","188"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:24:00Z"],["dc.date.available","2018-11-07T09:24:00Z"],["dc.date.issued","2013"],["dc.description.abstract","The gene nanos (nos) is a maternal-effect gene that plays an important role in posterior patterning and germ cell development in early stage embryos. nos is known from several diverse insect species, but has so far not been described for any Tephritid fruit fly. Here, we report the molecular cloning and expression pattern of the nos orthologous gene, Ccnos, in the Mediterranean fruit fly Ceratitis capitata, which is a destructive pest of high agricultural importance. CcNOS contains 398 amino acids and has a C-terminal region with two conserved CCHC zinc-binding motifs known to be essential for NOS function. Transcripts of Ccnos were confirmed by in situ hybridization to be maternally-derived and localized to the posterior pole of early stage embryos. Regulatory regions of nos have been employed in genetic engineering in some dipterans such as Drosophila and mosquitoes. Given the similarity in spatial and temporal expression between Ccnos and nos orthologs from other dipterans, its regulatory regions will be valuable to generate additional genetic tools that can be applied for engineering purposes to improve the fight against this devastating pest. (C) 2013 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1016/j.gep.2013.03.002"],["dc.identifier.isi","000320093300007"],["dc.identifier.pmid","23567755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29719"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1567-133X"],["dc.title","Molecular cloning and expression of nanos in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Insect Biochemistry and Molecular Biology"],["dc.bibliographiccitation.lastpage","8"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:30:36Z"],["dc.date.available","2018-11-07T09:30:36Z"],["dc.date.issued","2013"],["dc.description.abstract","Fruit fly pest species have been successfully controlled and managed via the Sterile Insect Technique (SIT), a control strategy that uses infertile matings of sterile males to wild females to reduce pest populations. Biological efficiency in the field is higher if only sterile males are released in SIT programs and production costs are also reduced. Sexing strains developed in the Mediterranean fruit fly Ceratitis capitata (medfly) through classical genetics are immensely beneficial to medfly SIT programs but exhibit reduced fertility and fitness. Moreover, transfer of such classical genetic systems to other tephritid species is difficult. Transgenic approaches can overcome this limitation of classical genetic sexing strains (GSSs), but had resulted so far in transgenic sexing strains (TSSs) with dominant lethality at late larval and pupal stages. Here we present a transgene-based female-specific lethality system for early embryonic sexing in medfly. The system utilizes the sex-specifically spliced transformer intron to restrict ectopic mRNA translation of the pro-apoptotic gene hid(Ala5) to females only. The expression of this lethal effector gene is driven by a tetracycline-repressible transactivator gene tTA that is under the control of promoters/enhancers of early-acting cellularization genes. Despite observed position effects on the sex-specific splicing, we could effectively establish this early-acting transgenic sexing system in the medfly C capitata. After satisfactory performance in large scale tests. TSSs based on this system will offer cost-effective sexing once introduced into SIT programs. Moreover, this approach is straight forward to be developed also for other insect pest and vector species. (C) 2012 Elsevier Ltd. All rights reserved."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1016/j.ibmb.2012.10.010"],["dc.identifier.isi","000313934800001"],["dc.identifier.pmid","23137881"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31344"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","0965-1748"],["dc.title","Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethality"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","16"],["dc.bibliographiccitation.journal","Insect Biochemistry and Molecular Biology"],["dc.bibliographiccitation.lastpage","24"],["dc.bibliographiccitation.volume","98"],["dc.contributor.author","Eckermann, Kolja N."],["dc.contributor.author","Ahmed, Hassan M.M."],["dc.contributor.author","Karami Nejad Ranjbar, Mohammad"],["dc.contributor.author","Dippel, Stefan"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Kitzmann, Peter"],["dc.contributor.author","Isah, Musa D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2020-12-10T14:24:26Z"],["dc.date.available","2020-12-10T14:24:26Z"],["dc.date.issued","2018"],["dc.identifier.doi","10.1016/j.ibmb.2018.04.001"],["dc.identifier.issn","0965-1748"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/72250"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.title","Hyperactive piggyBac transposase improves transformation efficiency in diverse insect species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]