Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","700"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Evolution & Development"],["dc.bibliographiccitation.lastpage","704"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Schmid, Bernhard G. M."],["dc.contributor.author","Zimowska, Grazyna"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:09:26Z"],["dc.date.available","2018-11-07T11:09:26Z"],["dc.date.issued","2008"],["dc.description.abstract","orthodenticle (otd) genes are found throughout the animal kingdom and encode well-studied homeodomain transcription factors that share conserved functions in cephalization, head segmentation, brain patterning, and the differentiation of photoreceptors. Otd proteins have been proposed as ancestral key players in anterior determination despite a high level of variation in gene expression at early developmental stages: otd is expressed strictly zygotically in the dipteran Drosophila melanogaster, while otd1 mRNA is contributed maternally to the embryo in the coleopteran Tribolium castaneum and maternal otd1 mRNA is localized to the anterior and posterior pole of the oocyte in the hymopteran Nasonia vitripennis. Here we demonstrate that such changes in otd mRNA expression and localization do not need to represent large phylogenetic distances but can occur even within closely related taxa. We show maternal otd expression in the medfly Ceratitis capitata and maternally localized otd mRNA in the caribfly Anastrepha suspensa, two cyclorrhaphan species closely related to Drosophila. This indicates considerable plasticity in expression and mRNA localization of key developmental genes even within short evolutionary distances."],["dc.identifier.isi","000260499000005"],["dc.identifier.pmid","19021740"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53006"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1520-541X"],["dc.title","Plasticity in mRNA expression and localization of orthodenticle within higher Diptera"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","S10"],["dc.bibliographiccitation.journal","BMC Genetics"],["dc.bibliographiccitation.volume","15"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Yuval, Boaz"],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Gabrieli, Paolo"],["dc.contributor.author","Bassetti, Federico"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Gasperi, Giuliano"],["dc.date.accessioned","2018-11-07T09:31:33Z"],["dc.date.available","2018-11-07T09:31:33Z"],["dc.date.issued","2014"],["dc.description.abstract","Background: In the Mediterranean fruit fly (medfly), Ceratitis capitata, a highly invasive agricultural pest species, polyandry, associated with sperm precedence, is a recurrent behaviour in the wild. The absence of tools for the unambiguous discrimination between competing sperm from different males in the complex female reproductive tract has strongly limited the understanding of mechanisms controlling sperm dynamics and use. Results: Here we use transgenic medfly lines expressing green or red fluorescent proteins in the spermatozoa, which can be easily observed and unambiguously differentiated within the female fertilization chamber. In twice-mated females, one day after the second mating, sperm from the first male appeared to be homogenously distributed all over the distal portion of each alveolus within the fertilization chamber, whereas sperm from the second male were clearly concentrated in the central portion of each alveolus. This distinct stratified sperm distribution was not maintained over time, as green and red sperm appeared homogeneously mixed seven days after the second mating. This dynamic sperm storage pattern is mirrored by the paternal contribution in the progeny of twice-mated females. Conclusions: Polyandrous medfly females, unlike Drosophila, conserve sperm from two different mates to fertilize their eggs. From an evolutionary point of view, the storage of sperm in a stratified pattern by medfly females may initially favour the fresher ejaculate from the second male. However, as the second male's sperm gradually becomes depleted, the sperm from the first male becomes increasingly available for fertilization. The accumulation of sperm from different males will increase the overall genetic variability of the offspring and will ultimately affect the effective population size. From an applicative point of view, the dynamics of sperm storage and their temporal use by a polyandrous female may have an impact on the Sterile Insect Technique (SIT). Indeed, even if the female's last mate is sterile, an increasing proportion of sperm from a previous mating with a fertile male may contribute to sire viable progeny."],["dc.identifier.doi","10.1186/1471-2156-15-S2-S10"],["dc.identifier.isi","000353980100011"],["dc.identifier.pmid","25470981"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13267"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31555"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1471-2156"],["dc.rights","CC BY 3.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/3.0"],["dc.title","Polyandry in the medfly - shifts in paternity mediated by sperm stratification and mixing"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","192"],["dc.bibliographiccitation.journal","Genome Biology"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Papanicolaou, Alexie"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Arensburger, Peter"],["dc.contributor.author","Atkinson, Peter W."],["dc.contributor.author","Benoit, Joshua B."],["dc.contributor.author","Bourtzis, Kostas"],["dc.contributor.author","Castanera, Pedro"],["dc.contributor.author","Cavanaugh, John P."],["dc.contributor.author","Chao, Hsu"],["dc.contributor.author","Childers, Christopher"],["dc.contributor.author","Curril, Ingrid"],["dc.contributor.author","Huyen Dinh, Huyen Dinh"],["dc.contributor.author","Doddapaneni, HarshaVardhan"],["dc.contributor.author","Dolan, Amanda"],["dc.contributor.author","Dugan, Shannon"],["dc.contributor.author","Friedrich, Markus"],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Geib, Scott"],["dc.contributor.author","Georgakilas, Georgios"],["dc.contributor.author","Gibbs, Richard A."],["dc.contributor.author","Giers, Sarah D."],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Gonzalez-Guzman, Miguel"],["dc.contributor.author","Guillem-Amat, Ana"],["dc.contributor.author","Han, Y. I."],["dc.contributor.author","Hatzigeorgiou, Artemis G."],["dc.contributor.author","Hernandez-Crespo, Pedro"],["dc.contributor.author","Hughes, Daniel S. T."],["dc.contributor.author","Jones, Jeffery W."],["dc.contributor.author","Karagkouni, Dimitra"],["dc.contributor.author","Koskinioti, Panagiota"],["dc.contributor.author","Lee, Sandra L."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Manni, Mose"],["dc.contributor.author","Mathiopoulos, Kostas"],["dc.contributor.author","Meccariello, Angela"],["dc.contributor.author","Murali, Shwetha C."],["dc.contributor.author","Murphy, Terence D."],["dc.contributor.author","Muzny, Donna M."],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Ortego, Felix"],["dc.contributor.author","Paraskevopoulou, Maria D."],["dc.contributor.author","Poelchau, Monica"],["dc.contributor.author","Qu, Jiaxin"],["dc.contributor.author","Reczko, Martin"],["dc.contributor.author","Robertson, Hugh M."],["dc.contributor.author","Rosendale, Andrew J."],["dc.contributor.author","Rosselot, Andrew E."],["dc.contributor.author","Saccone, Giuseppe"],["dc.contributor.author","Salvemini, Marco"],["dc.contributor.author","Savini, Grazia"],["dc.contributor.author","Schreiner, Patrick"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Siciliano, Paolo"],["dc.contributor.author","Sim, Sheina B."],["dc.contributor.author","Tsiamis, George"],["dc.contributor.author","Urena, Enric"],["dc.contributor.author","Vlachos, Ioannis S."],["dc.contributor.author","Werren, John H."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Worley, Kim C."],["dc.contributor.author","Zacharopoulou, Antigone"],["dc.contributor.author","Richards, Stephen J."],["dc.contributor.author","Handler, Alfred M."],["dc.date.accessioned","2018-11-07T10:08:20Z"],["dc.date.available","2018-11-07T10:08:20Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: The Mediterranean fruit fly (medfly), Ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. It exhibits a unique ability to invade and adapt to ecological niches throughout tropical and subtropical regions of the world, though medfly infestations have been prevented and controlled by the sterile insect technique (SIT) as part of integrated pest management programs (IPMs). The genetic analysis and manipulation of medfly has been subject to intensive study in an effort to improve SIT efficacy and other aspects of IPM control. Results: The 479 Mb medfly genome is sequenced from adult flies from lines inbred for 20 generations. A high-quality assembly is achieved having a contig N50 of 45.7 kb and scaffold N50 of 4.06 Mb. In-depth curation of more than 1800 messenger RNAs shows specific gene expansions that can be related to invasiveness and host adaptation, including gene families for chemoreception, toxin and insecticide metabolism, cuticle proteins, opsins, and aquaporins. We identify genes relevant to IPM control, including those required to improve SIT. Conclusions: The medfly genome sequence provides critical insights into the biology of one of the most serious and widespread agricultural pests. This knowledge should significantly advance the means of controlling the size and invasive potential of medfly populations. Its close relationship to Drosophila, and other insect species important to agriculture and human health, will further comparative functional and structural studies of insect genomes that should broaden our understanding of gene family evolution."],["dc.identifier.doi","10.1186/s13059-016-1049-2"],["dc.identifier.isi","000384461900002"],["dc.identifier.pmid","27659211"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13887"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39453"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1474-760X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","76"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","New Biotechnology"],["dc.bibliographiccitation.lastpage","84"],["dc.bibliographiccitation.volume","25"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Bertin, Sabrina"],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:14:51Z"],["dc.date.available","2018-11-07T11:14:51Z"],["dc.date.issued","2008"],["dc.description.abstract","The Sterile Insect Technique (SIT) involving area-wide release of mass-reared and sterilized pest insects has proven successful to reduce, control and eradicate economically important pest species, such as the Mediterranean fruit fly (medfly). For the efficient application, effective monitoring to assess the number and mating success of the released medflies is essential. Here, we report sperm-specific marking systems based on the spermatogenesis-specific Ceratitis capitata beta 2-tubulin (Cc beta 2t) promoter. Fluorescent sperm can be isolated from testes or spermathecae. The marking does not cause general disadvantages in preliminary laboratory competitiveness assays. Therefore, transgenic sperm marking could serve as a major improvement for monitoring medfly SIT programs. The use of such harmless transgenic markers will serve as an ideal initial condition to transfer insect transgenesis technology from the laboratory to field applications. Moreover, effective and easily recognizable sperm marking will make novel studies possible on medfly reproductive biology which will help to further improve SIT programs."],["dc.identifier.doi","10.1016/j.nbt.2008.02.001"],["dc.identifier.isi","000259343400022"],["dc.identifier.pmid","18504022"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54233"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1871-6784"],["dc.title","Fluorescent sperm marking to improve the fight against the pest insect Ceratitis capitata (Wiedemann; Diptera : Tephritidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Insect Biochemistry and Molecular Biology"],["dc.bibliographiccitation.lastpage","8"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:30:36Z"],["dc.date.available","2018-11-07T09:30:36Z"],["dc.date.issued","2013"],["dc.description.abstract","Fruit fly pest species have been successfully controlled and managed via the Sterile Insect Technique (SIT), a control strategy that uses infertile matings of sterile males to wild females to reduce pest populations. Biological efficiency in the field is higher if only sterile males are released in SIT programs and production costs are also reduced. Sexing strains developed in the Mediterranean fruit fly Ceratitis capitata (medfly) through classical genetics are immensely beneficial to medfly SIT programs but exhibit reduced fertility and fitness. Moreover, transfer of such classical genetic systems to other tephritid species is difficult. Transgenic approaches can overcome this limitation of classical genetic sexing strains (GSSs), but had resulted so far in transgenic sexing strains (TSSs) with dominant lethality at late larval and pupal stages. Here we present a transgene-based female-specific lethality system for early embryonic sexing in medfly. The system utilizes the sex-specifically spliced transformer intron to restrict ectopic mRNA translation of the pro-apoptotic gene hid(Ala5) to females only. The expression of this lethal effector gene is driven by a tetracycline-repressible transactivator gene tTA that is under the control of promoters/enhancers of early-acting cellularization genes. Despite observed position effects on the sex-specific splicing, we could effectively establish this early-acting transgenic sexing system in the medfly C capitata. After satisfactory performance in large scale tests. TSSs based on this system will offer cost-effective sexing once introduced into SIT programs. Moreover, this approach is straight forward to be developed also for other insect pest and vector species. (C) 2012 Elsevier Ltd. All rights reserved."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1016/j.ibmb.2012.10.010"],["dc.identifier.isi","000313934800001"],["dc.identifier.pmid","23137881"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/31344"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","0965-1748"],["dc.title","Transgenic sexing system for Ceratitis capitata (Diptera: Tephritidae) based on female-specific embryonic lethality"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","4"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Caceres, Carlos"],["dc.contributor.author","Zacharopoulou, Antigone"],["dc.contributor.author","Franz, Gerald"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T08:33:26Z"],["dc.date.available","2018-11-07T08:33:26Z"],["dc.date.issued","2009"],["dc.description.abstract","Background: The sterile insect technique (SIT) is an environment-friendly method used in area-wide pest management of the Mediterranean fruit fly Ceratitis capitata (Wiedemann; Diptera: Tephritidae). Ionizing radiation used to generate reproductive sterility in the mass-reared populations before release leads to reduction of competitiveness. Results: Here, we present a first alternative reproductive sterility system for medfly based on transgenic embryonic lethality. This system is dependent on newly isolated medfly promoter/enhancer elements of cellularization-specifically-expressed genes. These elements act differently in expression strength and their ability to drive lethal effector gene activation. Moreover, position effects strongly influence the efficiency of the system. Out of 60 combinations of driver and effector construct integrations, several lines resulted in larval and pupal lethality with one line showing complete embryonic lethality. This line was highly competitive to wildtype medfly in laboratory and field cage tests. Conclusion: The high competitiveness of the transgenic lines and the achieved 100% embryonic lethality causing reproductive sterility without the need of irradiation can improve the efficacy of operational medfly SIT programs."],["dc.identifier.doi","10.1186/1741-7007-7-4"],["dc.identifier.isi","000264767200001"],["dc.identifier.pmid","19173707"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5771"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17575"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1741-7007"],["dc.rights","CC BY 2.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.title","Conditional embryonic lethality to improve the sterile insect technique in Ceratitis capitata (Diptera: Tephritidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","820"],["dc.bibliographiccitation.issue","9-10"],["dc.bibliographiccitation.journal","Journal of Applied Entomology"],["dc.bibliographiccitation.lastpage","831"],["dc.bibliographiccitation.volume","132"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Gabrieli, Paolo"],["dc.contributor.author","Siciliano, Paolo"],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Karam, N."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Gasperi, Giuliano"],["dc.date.accessioned","2018-11-07T11:08:36Z"],["dc.date.available","2018-11-07T11:08:36Z"],["dc.date.issued","2008"],["dc.description.abstract","Tephritid fruit fly species cause major economical losses in crops worldwide. Genetic transformation of insect pests, which are targets of the Sterile Insect Technique (SIT), a key component of area-wide pest management, has been achieved for several years. For the medfly Ceratitis capitata as well as several Bactrocera and Anastrepha species, germline transformation can now be used to bioengineer strains that should increase the efficacy and cost-effectiveness of the SIT. Novel transformation vectors, robust genetic markers and diverse promoters to drive stage- and tissue-specific gene expression provide powerful tools to test the contribution that these technologies can make to current SIT programmes."],["dc.identifier.doi","10.1111/j.1439-0418.2008.01347.x"],["dc.identifier.isi","000262106200018"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52820"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1439-0418"],["dc.relation.issn","0931-2048"],["dc.title","Insect transgenesis applied to tephritid pest control"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","71"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Genetica"],["dc.bibliographiccitation.lastpage","78"],["dc.bibliographiccitation.volume","139"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Goetschel, Frank"],["dc.contributor.author","Viktorinova, Ivana"],["dc.contributor.author","Handler, Alfred M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:01:19Z"],["dc.date.available","2018-11-07T09:01:19Z"],["dc.date.issued","2011"],["dc.description.abstract","Transposon-based vectors currently provide the most suitable gene transfer systems for insect germ-line transformation and are used for molecular improvement of the Sterile Insect Technique. However, the long time stability of genome-integrated transposon constructs depends on the absence of transposase activity that could remobilize the transposon-embedded transgenes. To achieve transgene stability transposon vectors are usually non-autonomous, lacking a functional transposase gene, and chosen so that endogenous or related transposon activities are not present in the host. Nevertheless, the non-autonomous transposon-embedded transgenes could become unstable by the unintended presence of a mobilizing transposase that may have been undetected or subsequently entered the host species by horizontal gene transfer. Since the field release of transgenic insects will present environmental concerns relating to large populations and high mobility, it will be important to ensure that transgene constructs are stably integrated for maintaining strain integrity and eliminating the possibility for unintentional transfer into the genome of another organism. Here we review efficient methods to delete or rearrange terminal repeat sequences of transposons necessary for their mobility, subsequent to their initial genomic integration. These procedures should prevent transposase-mediated remobilization of the transgenes, ensuring their genomic stability."],["dc.identifier.doi","10.1007/s10709-010-9494-4"],["dc.identifier.isi","000286667400008"],["dc.identifier.pmid","20844938"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7361"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24399"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0016-6707"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Recombination technologies for enhanced transgene stability in bioengineered insects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","18171"],["dc.bibliographiccitation.issue","43"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","18176"],["dc.bibliographiccitation.volume","106"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Handler, Alfred M."],["dc.contributor.author","Kittelmann, Sebastian"],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:23:09Z"],["dc.date.available","2018-11-07T11:23:09Z"],["dc.date.issued","2009"],["dc.description.abstract","Insect transgenesis is mainly based on the random genomic integration of DNA fragments embedded into non-autonomous transposable elements. Once a random insertion into a specific location of the genome has been identified as particularly useful with respect to transgene expression, the ability to make the insertion homozygous, and lack of fitness costs, it may be advantageous to use that location for further modification. Here we describe an efficient method for the modification of previously inserted transgenes by the use of the site-specific integration system from phage phiC31 in a tephritid pest species, the Mediterranean fruit fly Ceratitis capitata. First, suitable transgenic strains with randomly integrated attP landing sites within transposon-based vectors were identified by molecular and functional characterization. Second, donor plasmids containing an attB site, with additional markers, and transposon ends were integrated into attP sites by phiC31 integrase-mediated recombination. Third, transposase-encoding 'jumpstarter' strains were created and mated to transgenic strains resulting in the postintegrational excision of transposon ends, which left stably integrated transgene insertions that could not be remobilized. This three-step integration and stabilization system will allow the combination of several transgene-encoded advantageous traits at evaluated genomic positions to generate optimized strains for pest control that minimize environmental concerns."],["dc.identifier.doi","10.1073/pnas.0907264106"],["dc.identifier.isi","000271222500026"],["dc.identifier.pmid","19828439"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56133"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","11"],["dc.bibliographiccitation.journal","Genome Biology"],["dc.bibliographiccitation.volume","18"],["dc.contributor.author","Papanicolaou, Alexie"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Arensburger, Peter"],["dc.contributor.author","Atkinson, Peter W."],["dc.contributor.author","Benoit, Joshua B."],["dc.contributor.author","Bourtzis, Kostas"],["dc.contributor.author","Castanera, Pedro"],["dc.contributor.author","Cavanaugh, John P."],["dc.contributor.author","Chao, Hsu"],["dc.contributor.author","Childers, Christopher"],["dc.contributor.author","Curril, Ingrid"],["dc.contributor.author","Dinh, Huyen"],["dc.contributor.author","Doddapaneni, HarshaVardhan"],["dc.contributor.author","Dolan, Amanda"],["dc.contributor.author","Dugan, Shannon"],["dc.contributor.author","Friedrich, Markus"],["dc.contributor.author","Gasperi, Giuliano"],["dc.contributor.author","Geib, Scott"],["dc.contributor.author","Georgakilas, Georgios"],["dc.contributor.author","Gibbs, Richard A."],["dc.contributor.author","Giers, Sarah D."],["dc.contributor.author","Gomulski, Ludvik M."],["dc.contributor.author","Gonzalez-Guzman, Miguel"],["dc.contributor.author","Guillem-Amat, Ana"],["dc.contributor.author","Han, Y. I."],["dc.contributor.author","Hatzigeorgiou, Artemis G."],["dc.contributor.author","Hernandez-Crespo, Pedro"],["dc.contributor.author","Hughes, Daniel S. T."],["dc.contributor.author","Jones, Jeffery W."],["dc.contributor.author","Karagkouni, Dimitra"],["dc.contributor.author","Koskinioti, Panagiota"],["dc.contributor.author","Lee, Sandra L."],["dc.contributor.author","Malacrida, Anna R."],["dc.contributor.author","Manni, Mose"],["dc.contributor.author","Mathiopoulos, Kostas"],["dc.contributor.author","Meccariello, Angela"],["dc.contributor.author","Munoz-Torres, Monica"],["dc.contributor.author","Murali, Shwetha C."],["dc.contributor.author","Murphy, Terence D."],["dc.contributor.author","Muzny, Donna M."],["dc.contributor.author","Oberhofer, Georg"],["dc.contributor.author","Ortego, Felix"],["dc.contributor.author","Paraskevopoulou, Maria D."],["dc.contributor.author","Poelchau, Monica"],["dc.contributor.author","Qu, Jiaxin"],["dc.contributor.author","Reczko, Martin"],["dc.contributor.author","Robertson, Hugh M."],["dc.contributor.author","Rosendale, Andrew J."],["dc.contributor.author","Rosselot, Andrew E."],["dc.contributor.author","Saccone, Giuseppe"],["dc.contributor.author","Salvemini, Marco"],["dc.contributor.author","Savini, Grazia"],["dc.contributor.author","Schreiner, Patrick"],["dc.contributor.author","Scolari, Francesca"],["dc.contributor.author","Siciliano, Paolo"],["dc.contributor.author","Sim, Sheina B."],["dc.contributor.author","Tsiamis, George"],["dc.contributor.author","Urena, Enric"],["dc.contributor.author","Vlachos, Ioannis S."],["dc.contributor.author","Werren, John H."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Worley, Kim C."],["dc.contributor.author","Zacharopoulou, Antigone"],["dc.contributor.author","Richards, Stephen J."],["dc.contributor.author","Handler, Alfred M."],["dc.date.accessioned","2018-11-07T10:28:20Z"],["dc.date.available","2018-11-07T10:28:20Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1186/s13059-017-1155-9"],["dc.identifier.isi","000394826300003"],["dc.identifier.pmid","28100280"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14361"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43399"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1474-760X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","The whole genome sequence of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species (vol 17, 192, 2016)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]