Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","4921"],["dc.bibliographiccitation.issue","13"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","4926"],["dc.bibliographiccitation.volume","109"],["dc.contributor.author","Khadjeh, Sara"],["dc.contributor.author","Turetzek, Natascha"],["dc.contributor.author","Pechmann, Matthias"],["dc.contributor.author","Schwager, Evelyn E."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Damen, Wim G. M."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T09:12:10Z"],["dc.date.available","2018-11-07T09:12:10Z"],["dc.date.issued","2012"],["dc.description.abstract","Evolution often results in morphologically similar solutions in different organisms, a phenomenon known as convergence. However, there is little knowledge of the processes that lead to convergence at the genetic level. The genes of the Hox cluster control morphology in animals. They may also be central to the convergence of morphological traits, but whether morphological similarities also require similar changes in Hox gene function is disputed. In arthropods, body subdivision into a region with locomotory appendages (\"thorax\") and a region with reduced appendages (\"abdomen\") has evolved convergently in several groups, e. g., spiders and insects. In insects, legs develop in the expression domain of the Hox gene Antennapedia (Antp), whereas the Hox genes Ultrabithorax (Ubx) and abdominal-A mediate leg repression in the abdomen. Here, we show that, unlike Antp in insects, the Antp gene in the spider Achaearanea tepidariorum represses legs in the first segment of the abdomen (opisthosoma), and that Antp and Ubx are redundant in the following segment. The down-regulation of Antp in A. tepidariorum leads to a striking 10-legged phenotype. We present evidence from ectopic expression of the spider Antp gene in Drosophila embryos and imaginal tissue that this unique function of Antp is not due to changes in the Antp protein, but likely due to divergent evolution of cofactors, Hox collaborators or target genes in spiders and flies. Our results illustrate an interesting example of convergent evolution of abdominal leg repression in arthropods by altering the role of distinct Hox genes at different levels of their action."],["dc.identifier.doi","10.1073/pnas.1116421109"],["dc.identifier.isi","000302164200046"],["dc.identifier.pmid","22421434"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/26887"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.title","Divergent role of the Hox gene Antennapedia in spiders is responsible for the convergent evolution of abdominal limb repression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","700"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Evolution & Development"],["dc.bibliographiccitation.lastpage","704"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Schetelig, Marc F."],["dc.contributor.author","Schmid, Bernhard G. M."],["dc.contributor.author","Zimowska, Grazyna"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:09:26Z"],["dc.date.available","2018-11-07T11:09:26Z"],["dc.date.issued","2008"],["dc.description.abstract","orthodenticle (otd) genes are found throughout the animal kingdom and encode well-studied homeodomain transcription factors that share conserved functions in cephalization, head segmentation, brain patterning, and the differentiation of photoreceptors. Otd proteins have been proposed as ancestral key players in anterior determination despite a high level of variation in gene expression at early developmental stages: otd is expressed strictly zygotically in the dipteran Drosophila melanogaster, while otd1 mRNA is contributed maternally to the embryo in the coleopteran Tribolium castaneum and maternal otd1 mRNA is localized to the anterior and posterior pole of the oocyte in the hymopteran Nasonia vitripennis. Here we demonstrate that such changes in otd mRNA expression and localization do not need to represent large phylogenetic distances but can occur even within closely related taxa. We show maternal otd expression in the medfly Ceratitis capitata and maternally localized otd mRNA in the caribfly Anastrepha suspensa, two cyclorrhaphan species closely related to Drosophila. This indicates considerable plasticity in expression and mRNA localization of key developmental genes even within short evolutionary distances."],["dc.identifier.isi","000260499000005"],["dc.identifier.pmid","19021740"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53006"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1520-541X"],["dc.title","Plasticity in mRNA expression and localization of orthodenticle within higher Diptera"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","468"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Journal of Applied Entomology"],["dc.bibliographiccitation.lastpage","473"],["dc.bibliographiccitation.volume","138"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Curril, Ingrid M."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:38:30Z"],["dc.date.available","2018-11-07T09:38:30Z"],["dc.date.issued","2014"],["dc.description.abstract","Molecular technologies have enabled the generation of various transgenic insect strains for the area-wide control of agricultural pests and vectors of human diseases. Individual maintenance of several diverse transgenic lines or strains involves a lot of resources, and sometimes problems arise that threaten the strains being maintained. Here, we present a way to cost-effectively maintain transgenic lines or strains of the Mediterranean fruit fly Ceratitis capitata by extending their generation time. Immature stages were kept at 20 degrees C instead of 28 degrees C, and the subsequent generation of transgenic flies kept at different temperatures were found to have laboratory fecundity comparable to the untreated transgenic flies. Extension of generation time offers inexpensive strain maintenance as it reduces the resources, time and energy spent on maintenance of transgenic medfly strains, in addition to minimizing exposure of strains to problems sometimes associated with strain maintenance."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1111/jen.12040"],["dc.identifier.isi","000337695900011"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33077"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1439-0418"],["dc.relation.issn","0931-2048"],["dc.title","Generation time extension for cost-effective strain maintenance of transgenic Mediterranean fruit fly, Ceratitis capitata [Diptera: Tephritidae]"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","341"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","350"],["dc.bibliographiccitation.volume","223"],["dc.contributor.author","Schaeper, Nina D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T09:18:17Z"],["dc.date.available","2018-11-07T09:18:17Z"],["dc.date.issued","2013"],["dc.description.abstract","Arthropod appendages are among the most diverse animal organs and have been adapted to a variety of functions. Due to this diversity, it can be difficult to recognize homologous parts in different appendage types and different species. Gene expression patterns of appendage development genes have been used to overcome this problem and to identify homologous limb portions across different species and their appendages. However, regarding the largest arthropod group, the hexapods, most of these studies focused on members of the winged insects (Pterygota), but primitively wingless groups like the springtails (Collembola) or silverfish and allies (Zygentoma) are underrepresented. We have studied the expression of a set of appendage patterning genes in the firebrat Thermobia domestica and the white springtail Folsomia candida. The expressions of Distal-less (Dll) and dachshund (dac) are generally similar to the patterns reported for pterygote insects. Modifications of gene regulation, for example, the lack of Dll expression in the palp of F. candida mouthparts, however, point to changes in gene function that can make the use of single genes and specific expression domains problematic for homology inference. Such hypotheses should therefore not rely on a small number of genes and should ideally also include information about gene function. The expression patterns of homothorax (hth) and extradenticle (exd) in both species are similar to the patterns of crustaceans and pterygote insects, but differ from those in chelicerates and myriapods. The proximal specificity of hth thus appears to trace from a common hexapod ancestor and also provides a link to the regulation of this gene in crustaceans."],["dc.identifier.doi","10.1007/s00427-013-0449-5"],["dc.identifier.isi","000325810600001"],["dc.identifier.pmid","23873479"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28374"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","1432-041X"],["dc.relation.issn","0949-944X"],["dc.title","Appendage patterning in the primitively wingless hexapods Thermobia domestica (Zygentoma: Lepismatidae) and Folsomia candida (Collembola: Isotomidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","183"],["dc.bibliographiccitation.issue","5-6"],["dc.bibliographiccitation.journal","Gene Expression Patterns"],["dc.bibliographiccitation.lastpage","188"],["dc.bibliographiccitation.volume","13"],["dc.contributor.author","Ogaugwu, Christian E."],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T09:24:00Z"],["dc.date.available","2018-11-07T09:24:00Z"],["dc.date.issued","2013"],["dc.description.abstract","The gene nanos (nos) is a maternal-effect gene that plays an important role in posterior patterning and germ cell development in early stage embryos. nos is known from several diverse insect species, but has so far not been described for any Tephritid fruit fly. Here, we report the molecular cloning and expression pattern of the nos orthologous gene, Ccnos, in the Mediterranean fruit fly Ceratitis capitata, which is a destructive pest of high agricultural importance. CcNOS contains 398 amino acids and has a C-terminal region with two conserved CCHC zinc-binding motifs known to be essential for NOS function. Transcripts of Ccnos were confirmed by in situ hybridization to be maternally-derived and localized to the posterior pole of early stage embryos. Regulatory regions of nos have been employed in genetic engineering in some dipterans such as Drosophila and mosquitoes. Given the similarity in spatial and temporal expression between Ccnos and nos orthologs from other dipterans, its regulatory regions will be valuable to generate additional genetic tools that can be applied for engineering purposes to improve the fight against this devastating pest. (C) 2013 Elsevier B.V. All rights reserved."],["dc.description.sponsorship","German Academic Exchange Service (DAAD)"],["dc.identifier.doi","10.1016/j.gep.2013.03.002"],["dc.identifier.isi","000320093300007"],["dc.identifier.pmid","23567755"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29719"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","1567-133X"],["dc.title","Molecular cloning and expression of nanos in the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","432"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Nature Biotechnology"],["dc.bibliographiccitation.lastpage","433"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:13:00Z"],["dc.date.available","2018-11-07T11:13:00Z"],["dc.date.issued","2005"],["dc.description.abstract","Genetic engineering promises to improve a technique for controlling medflies that avoids chemical insecticides."],["dc.identifier.doi","10.1038/nbt0405-432"],["dc.identifier.isi","000228197300019"],["dc.identifier.pmid","15815668"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53793"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1087-0156"],["dc.title","Eco-friendly insect management"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","580"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","582"],["dc.bibliographiccitation.volume","2"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T11:11:24Z"],["dc.date.available","2018-11-07T11:11:24Z"],["dc.date.issued","2005"],["dc.description.abstract","Targeted genomic insertion wilt improve the qualitative and quantitative functional comparison of similar transgenes and provide suitable integration points for transgenes of applied interest."],["dc.identifier.doi","10.1038/nmeth0805-580"],["dc.identifier.isi","000230884500013"],["dc.identifier.pmid","16094381"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53426"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Nature Publishing Group"],["dc.relation.issn","1548-7091"],["dc.title","Insect transgenesis by site-specific recombination"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","853"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Trends in Biotechnology"],["dc.bibliographiccitation.lastpage","856"],["dc.bibliographiccitation.volume","39"],["dc.contributor.author","Devos, Yann"],["dc.contributor.author","Bonsall, Michael B."],["dc.contributor.author","Firbank, Leslie G."],["dc.contributor.author","Mumford, John"],["dc.contributor.author","Nogué, Fabien"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2021-09-01T06:42:29Z"],["dc.date.available","2021-09-01T06:42:29Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.tibtech.2020.11.015"],["dc.identifier.pii","S0167779920303103"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/89067"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-455"],["dc.relation.issn","0167-7799"],["dc.title","Gene Drive-Modified Organisms: Developing Practical Risk Assessment Guidance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","403"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Developmental Biology"],["dc.bibliographiccitation.lastpage","414"],["dc.bibliographiccitation.volume","360"],["dc.contributor.author","Ntini, Evgenia"],["dc.contributor.author","Wimmer, Ernst A."],["dc.date.accessioned","2018-11-07T08:48:53Z"],["dc.date.available","2018-11-07T08:48:53Z"],["dc.date.issued","2011"],["dc.description.abstract","In Drosophila, trunk metamerization is established by a cascade of segmentation gene activities: the gap genes, the pair rule genes, and the segment polarity genes. In the anterior head, metamerization requires also gap-like genes and segment polarity genes. However, because the pair rule genes are not active in this part of the embryo, the question on which gene activities are fulfilling the role of the second order regulator genes still remains to be solved. Here we provide first molecular evidence that the Helix-Loop-Helix-COE transcription factor Collier fulfills this role by directly activating the expression of the segment polarity gene hedgehog in the posterior part of the intercalary segment. Collier thereby occupies a newly identified binding site within an intercalary-specific cis-regulatory element. Moreover, we identified a direct physical association between Collier and the basic-leucine-zipper transcription factor Cap'n'collar B, which seems to restrict the activating input of Collier to the posterior part of the intercalary segment and to lead to the attenuation of hedgehog expression in the intercalary lobes at later stages. (C) 2011 Elsevier Inc. All rights reserved."],["dc.description.sponsorship","European Community [MRTN-CT-2004-005624]"],["dc.identifier.doi","10.1016/j.ydbio.2011.09.035"],["dc.identifier.isi","000297531600014"],["dc.identifier.pmid","22005665"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/21327"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Academic Press Inc Elsevier Science"],["dc.relation.issn","0012-1606"],["dc.title","Second order regulator Collier directly controls intercalary-specific segment polarity gene expression"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","257"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.volume","226"],["dc.contributor.author","Siomava, Natalia"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Posnien, Nico"],["dc.date.accessioned","2018-11-07T10:13:30Z"],["dc.date.available","2018-11-07T10:13:30Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1007/s00427-016-0553-4"],["dc.identifier.isi","000377362100012"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40445"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.iserratumof","/handle/2/40444"],["dc.relation.issn","1432-041X"],["dc.relation.issn","0949-944X"],["dc.title","Erratum to: Size relationships of different body parts in the three dipteran species Drosophila melanogaster, Ceratitis capitata and Musca domestica"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","erratum_ja"],["dspace.entity.type","Publication"]]