Wimmer, Ernst A.

[["dc.bibliographiccitation.firstpage","399"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Development Genes and Evolution"],["dc.bibliographiccitation.lastpage","407"],["dc.bibliographiccitation.volume","219"],["dc.contributor.author","Kittelmann, Maike"],["dc.contributor.author","Schinko, Johannes B."],["dc.contributor.author","Winkler, Marco"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Prpic, Nikola-Michael"],["dc.date.accessioned","2018-11-07T11:25:55Z"],["dc.date.available","2018-11-07T11:25:55Z"],["dc.date.issued","2009"],["dc.description.abstract","The genetic control of leg development is well characterized in the fly Drosophila melanogaster. These control mechanisms, however, must differ to some degree between different insect species to account for the morphological diversity of thoracic legs in the insects. The legs of the flour beetle Tribolium castaneum differ from the Drosophila legs in their developmental mode as well as in their specific morphology especially at the larval stage. In order to identify genes involved in the morphogenesis of the Tribolium larval legs, we have analyzed EGFP enhancer trap lines of Tribolium. We have identified the zfh2 gene as a novel factor required for normal leg development in Tribolium. RNA interference with zfh2 function leads to two alternative classes of leg phenotype. The loss of a leg segment boundary and the generation of ectopic outgrowths in one class of phenotype suggest a role in leg segmentation and segment growth. The malformation of the pretarsal claw in the second class of phenotype suggests a role in distal development and the morphogenesis of the claw-shaped morphology of the pretarsus. This suggests that zfh2 is involved in the regulation of an unidentified target gene in a concentration-dependent manner. Our results demonstrate that enhancer trap screens in T. castaneum have the potential to identify novel gene functions regulating specific developmental processes."],["dc.identifier.doi","10.1007/s00427-009-0303-y"],["dc.identifier.isi","000271397600002"],["dc.identifier.pmid","19760181"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/3758"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/56738"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0949-944X"],["dc.rights.access","openAccess"],["dc.subject.ddc","570"],["dc.title","Insertional mutagenesis screening identifies the zinc finger homeodomain 2 (zfh2) gene as a novel factor required for embryonic leg development in Tribolium castaneum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","73"],["dc.bibliographiccitation.journal","BMC Biology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Trauner, Jochen"],["dc.contributor.author","Schinko, Johannes"],["dc.contributor.author","Lorenzen, Marce D."],["dc.contributor.author","Shippy, Teresa D."],["dc.contributor.author","Wimmer, Ernst A."],["dc.contributor.author","Beeman, Richard W."],["dc.contributor.author","Klingler, Martin"],["dc.contributor.author","Bucher, Gregor"],["dc.contributor.author","Brown, Susan J."],["dc.date.accessioned","2018-11-07T11:22:11Z"],["dc.date.available","2018-11-07T11:22:11Z"],["dc.date.issued","2009"],["dc.description.abstract","Background: Given its sequenced genome and efficient systemic RNA interference response, the red flour beetle Tribolium castaneum is a model organism well suited for reverse genetics. Even so, there is a pressing need for forward genetic analysis to escape the bias inherent in candidate gene approaches. Results: To produce easy-to-maintain insertional mutations and to obtain fluorescent marker lines to aid phenotypic analysis, we undertook a large-scale transposon mutagenesis screen. In this screen, we produced more than 6,500 new piggyBac insertions. Of these, 421 proved to be recessive lethal, 75 were semi-lethal, and eight indicated recessive sterility, while 505 showed new enhancer-trap patterns. Insertion junctions were determined for 403 lines and often appeared to be located within transcription units. Insertion sites appeared to be randomly distributed throughout the genome, with the exception of a preference for reinsertion near the donor site. Conclusion: A large collection of enhancer-trap and embryonic lethal beetle lines has been made available to the research community and will foster investigations into diverse fields of insect biology, pest control, and evolution. Because the genetic elements used in this screen are species-nonspecific, and because the crossing scheme does not depend on balancer chromosomes, the methods presented herein should be broadly applicable for many insect species."],["dc.identifier.doi","10.1186/1741-7007-7-73"],["dc.identifier.fs","568930"],["dc.identifier.isi","000272336000001"],["dc.identifier.pmid","19891766"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/5844"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55941"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biomed Central Ltd"],["dc.relation.issn","1741-7007"],["dc.rights","CC BY 2.0"],["dc.rights.access","openAccess"],["dc.rights.uri","https://creativecommons.org/licenses/by/2.0"],["dc.subject.ddc","570"],["dc.subject.mesh","Animals"],["dc.subject.mesh","Chromosome Mapping"],["dc.subject.mesh","Crosses, Genetic"],["dc.subject.mesh","DNA Transposable Elements"],["dc.subject.mesh","Embryo Loss"],["dc.subject.mesh","Embryo, Nonmammalian"],["dc.subject.mesh","Enhancer Elements, Genetic"],["dc.subject.mesh","Genes, Lethal"],["dc.subject.mesh","Genetic Markers"],["dc.subject.mesh","Germ-Line Mutation"],["dc.subject.mesh","Mutagenesis, Insertional"],["dc.subject.mesh","Transposases"],["dc.subject.mesh","Tribolium"],["dc.title","Large-scale insertional mutagenesis of a coleopteran stored grain pest, the red flour beetle Tribolium castaneum, identifies embryonic lethal mutations and enhancer traps"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]