Braus, Gerhard H.

[["dc.bibliographiccitation.firstpage","S91"],["dc.bibliographiccitation.journal","Medical Mycology"],["dc.bibliographiccitation.lastpage","S94"],["dc.bibliographiccitation.volume","44"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Sasse, Christoph"],["dc.contributor.author","Krappmann, Sven"],["dc.date.accessioned","2018-11-07T09:22:02Z"],["dc.date.available","2018-11-07T09:22:02Z"],["dc.date.issued","2006"],["dc.description.abstract","Supply of all amino acids required for translation is crucial for the synthesis of new proteins. Fungal amino acid biosynthesis has to be coordinated with amino acid uptake as well as protein degradation. A global regulator that connects amino acid biosynthesis and developmental programs is the transcription factor CpcA/Gcn4p. This transcriptional activator is conserved within the fungal kingdom and the cellular levels of this protein are carefully regulated. Deletion of the encoding cpcA gene in the opportunistic pathogen Aspergillus fumigatus results in impaired virulence in immuno-compromised mice, suggesting a role of the cross-pathway control system in fungal pathogenicity."],["dc.identifier.doi","10.1080/13693780600898029"],["dc.identifier.isi","000242601400017"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/29246"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Taylor & Francis Ltd"],["dc.publisher.place","Abingdon"],["dc.relation.conference","2nd Advances against Aspergillosis Conference"],["dc.relation.eventlocation","Athens, GREECE"],["dc.relation.issn","1369-3786"],["dc.title","Amino acid acquisition, cross-pathway control, and virulence in Aspergillus"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1298"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Eukaryotic Cell"],["dc.bibliographiccitation.lastpage","1307"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Bayram, O."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T09:03:04Z"],["dc.date.available","2018-11-07T09:03:04Z"],["dc.date.issued","2005"],["dc.description.abstract","Detailed evaluation of gene functions in an asexual fungus requires advanced methods of molecular biology. For the generation of targeted gene deletions in the opportunistic pathogen Aspergillus fumigatus we designed a novel blaster module allowing dominant selection of transformants due to resistance to phleomycin as well as dominant (counter) selection of a Cre recombinase-mediated marker excision event. For validation purposes we have deleted the A. fumigatus pabaA gene in a wild-type isolate by making use of this cassette. The resulting pabaA::loxP strain served as the recipient for subsequent targeting of the velvet locus. Homologous reconstitution of the deleted gene was performed by an allele whose expression is driven in a nitrogen source-dependent manner, as validated by Northern analyses. Overexpression of the veA locus in A. fumigatus does not result in any obvious phenotype, whereas the sporulation capacities of the veA null mutant are reduced on nitrate-containing medium, a phenotype that is completely restored in the reconstituted strain."],["dc.identifier.doi","10.1128/EC.4.7.1298-1307.2005"],["dc.identifier.isi","000230609600014"],["dc.identifier.pmid","16002655"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/24823"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1535-9786"],["dc.relation.issn","1535-9778"],["dc.title","Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","6"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Fungal Biology and Biotechnology"],["dc.bibliographiccitation.volume","3"],["dc.contributor.author","Meyer, Vera"],["dc.contributor.author","Andersen, Mikael R."],["dc.contributor.author","Brakhage, Axel A."],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Caddick, Mark X."],["dc.contributor.author","Cairns, Timothy C."],["dc.contributor.author","de Vries, Ronald P."],["dc.contributor.author","Haarmann, Thomas"],["dc.contributor.author","Hansen, Kim"],["dc.contributor.author","Hertz-Fowler, Christiane"],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Mortensen, Uffe H."],["dc.contributor.author","Peñalva, Miguel A."],["dc.contributor.author","Ram, Arthur F. J."],["dc.contributor.author","Head, Ritchie M."],["dc.date.accessioned","2019-07-09T11:42:51Z"],["dc.date.available","2019-07-09T11:42:51Z"],["dc.date.issued","2016"],["dc.description.abstract","Abstract The EUROFUNG network is a virtual centre of multidisciplinary expertise in the field of fungal biotechnology. The first academic-industry Think Tank was hosted by EUROFUNG to summarise the state of the art and future challenges in fungal biology and biotechnology in the coming decade. Currently, fungal cell factories are important for bulk manufacturing of organic acids, proteins, enzymes, secondary metabolites and active pharmaceutical ingredients in white and red biotechnology. In contrast, fungal pathogens of humans kill more people than malaria or tuberculosis. Fungi are significantly impacting on global food security, damaging global crop production, causing disease in domesticated animals, and spoiling an estimated 10 % of harvested crops. A number of challenges now need to be addressed to improve our strategies to control fungal pathogenicity and to optimise the use of fungi as sources for novel compounds and as cell factories for large scale manufacture of bio-based products. This white paper reports on the discussions of the Think Tank meeting and the suggestions made for moving fungal bio(techno)logy forward."],["dc.identifier.doi","10.1186/s40694-016-0024-8"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13875"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/58764"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Current challenges of research on filamentous fungi in relation to human welfare and a sustainable bio-economy: a white paper"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","785"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Molecular Microbiology"],["dc.bibliographiccitation.lastpage","799"],["dc.bibliographiccitation.volume","52"],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Bignell, Elaine M."],["dc.contributor.author","Reichard, Utz"],["dc.contributor.author","Rogers, T."],["dc.contributor.author","Haynes, K."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T10:49:24Z"],["dc.date.available","2018-11-07T10:49:24Z"],["dc.date.issued","2004"],["dc.description.abstract","We have cloned and characterized the Aspergillus fumigatus cpcA gene encoding the transcriptional activator of the cross-pathway control system of amino acid biosynthesis. cpcA encodes a functional orthologue of Saccharomyces cerevisiae Gcn4p. The coding sequence of the 2.2 kb transcript is preceded by two short upstream open reading frames, the larger one being well conserved among Aspergilli. Deletion strains in which either the coding sequence or the entire locus are replaced by a bifunctional dominant marker are impaired in their cross-pathway control response upon amino acid starvation, as demonstrated by analyses of selected reporter genes and specific enzymatic activities. In a murine model of pulmonary aspergillosis, cpcADelta strains display attenuated virulence. Pathogenicity is restored to wild-type levels in strains with reconstitution of the genomic locus. Competitive mixed infection experiments additionally demonstrate that cpcADelta strains are less able to survive in vivo than their wild-type progenitor. Our data suggest that specific stress conditions are encountered by A. fumigatus within the mammalian host and that the fungal cross-pathway control system plays a significant role in pulmonary aspergillosis."],["dc.identifier.doi","10.1111/j.1365-2958.2004.04015.x"],["dc.identifier.isi","000220941400016"],["dc.identifier.pmid","15101984"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48419"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1365-2958"],["dc.relation.issn","0950-382X"],["dc.title","The Aspergillus fumigatus transcriptional activator CpcA contributes significantly to the virulence of this fungal pathogen"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","404"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Microbiology and Molecular Biology Reviews"],["dc.bibliographiccitation.lastpage","+"],["dc.bibliographiccitation.volume","65"],["dc.contributor.author","Helmstaedt, K."],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T08:43:40Z"],["dc.date.available","2018-11-07T08:43:40Z"],["dc.date.issued","2001"],["dc.description.abstract","Allosteric regulation of key metabolic enzymes is a fascinating field to study the structure-function relationship of induced conformational changes of proteins. In this review we compare the principles of allosteric transitions of the complex classical model aspartate transcarbamoylase (ATCase) from Escherichia coli, consisting of 12 polypetides, and the less complicated chorismate mutase derived from baker's yeast, which functions as a homodimer. Chorismate mutase presumably represents the minimal oligomerization state of a cooperative enzyme which still can be either activated or inhibited by different heterotropic effectors. Detailed knowledge of the number of possible quaternary states and a description of molecular triggers for conformational changes of model enzymes such as ATCase and chorismate mutase shed more and more light on allostery as an important regulatory mechanism of any living cell. The comparison of wild-type and engineered mutant enzymes reveals that current textbook models for regulation do not cover the entire picture needed to describe the function of these enzymes in detail."],["dc.identifier.doi","10.1128/MMBR.65.3.404-421.2001"],["dc.identifier.isi","000170874500005"],["dc.identifier.pmid","11528003"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/20022"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1092-2172"],["dc.title","Allosteric regulation of catalytic activity: Escherichia coli aspartate transcarbamoylase versus yeast chorismate mutase"],["dc.type","review"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3254"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Molecular Biology of the Cell"],["dc.bibliographiccitation.lastpage","3262"],["dc.bibliographiccitation.volume","19"],["dc.contributor.author","Bayram, Oezguer"],["dc.contributor.author","Biesemann, Christoph"],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Galland, Paul"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T11:12:39Z"],["dc.date.available","2018-11-07T11:12:39Z"],["dc.date.issued","2008"],["dc.description.abstract","Cryptochromes are blue-light receptors that have presumably evolved from the DNA photolyase protein family, and the genomes of many organisms contain genes for both types of molecules. Both protein structures resemble each other, which suggests that light control and light protection share a common ancient origin. In the genome of the filamentous fungus Aspergillus nidulans, however, only one cryptochrome/photolyase-encoding gene, termed cryA, was identified. Deletion of the cryA gene triggers sexual differentiation under inappropriate culture conditions and results in upregulation of transcripts encoding regulators of fruiting body formation. CryA is a protein whose N- and C-terminal synthetic green fluorescent protein fusions localize to the nucleus. CryA represses sexual development under UVA(350-370nm) light both on plates and in submerged culture. Strikingly, CryA exhibits photorepair activity as demonstrated by heterologous complementation of a DNA repair-deficient Escherichia coli strain as well as overexpression in an A. nidulans uvsB Delta genetic background. This is in contrast to the single deletion cryA Delta strain, which does not show increased sensitivity toward UV-induced damage. In A. nidulans, cryA encodes a novel type of cryptochrome/photolyase that exhibits a regulatory function during light-dependent development and DNA repair activity. This represents a paradigm for the evolutionary transition between photolyases and cryptochromes."],["dc.identifier.doi","10.1091/mbc.E08-01-0061"],["dc.identifier.isi","000259160400008"],["dc.identifier.pmid","18495868"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53714"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Cell Biology"],["dc.relation.issn","1059-1524"],["dc.title","More than a repair enzyme: Aspergillus nidulans photolyase-like CryA is a regulator of sexual development"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1268"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Eukaryotic Cell"],["dc.bibliographiccitation.lastpage","1277"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Schmalhorst, Philipp S."],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Vervecken, Wouter"],["dc.contributor.author","Rohde, Manfred"],["dc.contributor.author","Mueller, Meike"],["dc.contributor.author","Braus, Gerhard H."],["dc.contributor.author","Contreras, Roland"],["dc.contributor.author","Braun, Armin"],["dc.contributor.author","Bakker, Hans"],["dc.contributor.author","Routier, Francoise H."],["dc.date.accessioned","2018-11-07T11:12:21Z"],["dc.date.available","2018-11-07T11:12:21Z"],["dc.date.issued","2008"],["dc.description.abstract","The filamentous fungus Aspergillus fumigatus is responsible for a lethal disease called invasive aspergillosis that affects immunocompromised patients. This disease, like other human fungal diseases, is generally treated by compounds targeting the primary fungal cell membrane sterol. Recently, glucan synthesis inhibitors were added to the limited antifungal arsenal and encouraged the search for novel targets in cell wall biosynthesis. Although galactomannan is a major component of the A. fumigatus cell wall and extracellular matrix, the biosynthesis and role of galactomannan are currently unknown. By a targeted gene deletion approach, we demonstrate that UDP-galactopyranose mutase, a key enzyme of galactofuranose metabolism, controls the biosynthesis of galactomannan and galactofuranose containing glycoconjugates. The glfA deletion mutant generated in this study is devoid of galactofuranose and displays attenuated virulence in a low-dose mouse model of invasive aspergillosis that likely reflects the impaired growth of the mutant at mammalian body temperature. Furthermore, the absence of galactofuranose results in a thinner cell wall that correlates with an increased susceptibility to several antifungal agents. The UDP-galactopyranose mutase thus appears to be an appealing adjunct therapeutic target in combination with other drugs against A. fumigatus. Its absence from mammalian cells indeed offers a considerable advantage to achieve therapeutic selectivity."],["dc.identifier.doi","10.1128/EC.00109-08"],["dc.identifier.isi","000258666500005"],["dc.identifier.pmid","18552284"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53645"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","1535-9778"],["dc.title","Contribution of galactofuranose to the virulence of the opportunistic pathogen Aspergillus fumigatus"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","76"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Molecular Microbiology"],["dc.bibliographiccitation.lastpage","88"],["dc.bibliographiccitation.volume","61"],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Jung, Nadja"],["dc.contributor.author","Medic, Branka"],["dc.contributor.author","Busch, Silke"],["dc.contributor.author","Prade, Rolf A."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T09:38:23Z"],["dc.date.available","2018-11-07T09:38:23Z"],["dc.date.issued","2006"],["dc.description.abstract","Cellular differentiation relies on precise and controlled means of gene expression that act on several levels to ensure a flexible and defined spatio-temporal expression of a given gene product. In our aim to identify transcripts enriched during fruiting body formation of the homothallic ascomycete Aspergillus (Emericella) nidulans, the grrA gene could be identified in a negative subtraction hybridization screening procedure. It encodes a protein similar to fungal F-box proteins, which function as substrate receptors for ubiquitin ligases, and that is highly related to the Saccharomyces cerevisiae regulatory protein Grr1p. Expression studies confirmed induction of grrA transcription and expression of its gene product during cleistothecial development of A. nidulans. Functional complementation of a yeast grr1 Delta mutant was achieved by overexpression of the grrA coding sequence. A grrA Delta deletion mutant resembles the wild-type in hyphal growth, asexual sporulation, Hulle cell formation or development of asci-containing cleistothecia, but is unable to produce mature ascospores due to a block in meiosis as demonstrated by cytological staining of cleistothecial contents. Our results specify a particular involvement of the E3 ubiquitin ligase SCFGrrA in meiosis and sexual spore formation of an ascomyceteous fungus and shed light on the diverse functions of ubiquitin-proteasome-mediated protein degradation in eukaryotic development."],["dc.identifier.doi","10.1111/j.1365-2958.2006.05215.x"],["dc.identifier.isi","000238353300008"],["dc.identifier.pmid","16824096"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33058"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell"],["dc.relation.issn","1365-2958"],["dc.relation.issn","0950-382X"],["dc.title","The Aspergillus nidulans F-box protein GrrA links SCF activity to meiosis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","717"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Molecular Microbiology"],["dc.bibliographiccitation.lastpage","730"],["dc.bibliographiccitation.volume","49"],["dc.contributor.author","Busch, S."],["dc.contributor.author","Eckert, S. E."],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T10:37:24Z"],["dc.date.available","2018-11-07T10:37:24Z"],["dc.date.issued","2003"],["dc.description.abstract","The COP9 signalosome (CSN) is a conserved multiprotein complex involved in regulation of eukaryotic development. The deduced amino acid sequences of two Aspergillus nidulans genes, csnD and csnE, show high identities to the fourth and fifth CSN subunits of higher eukaryotes. The csnD transcript is abundant during vegetative growth as well as development and the corresponding protein accumulates in the nucleus. Strains deleted for either csn gene are viable and show identical mutant phenotypes at conditions that allow development: hyphae appear partly red and contain cells of reduced size. Additionally, light dependence of propagation onset is affected. The Deltacsn mutants are capable of initiating the sexual cycle and develop primordia, but maturation to sexual fruit bodies is blocked. This developmental arrest could not be overcome by overexpression of the sexual activator velvet (VEA). We conclude that the COP9 signalosome in A. nidulans is a key regulator of sexual development, and its proposed structural and functional conservation to the CSN of higher eukaryotes enables studies on this regulatory complex in a genetically amenable organism."],["dc.identifier.doi","10.1046/j.1365-2958.2003.03612.x"],["dc.identifier.isi","000184674100011"],["dc.identifier.pmid","12864854"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45558"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing Ltd"],["dc.relation.issn","0950-382X"],["dc.title","The COP9 signalosome is an essential regulator of development in the filamentous fungus Aspergillus nidulans"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1504"],["dc.bibliographiccitation.issue","5882"],["dc.bibliographiccitation.journal","Science"],["dc.bibliographiccitation.lastpage","1506"],["dc.bibliographiccitation.volume","320"],["dc.contributor.author","Bayram, Oezguer"],["dc.contributor.author","Krappmann, Sven"],["dc.contributor.author","Ni, Min"],["dc.contributor.author","Bok, Jin Woo"],["dc.contributor.author","Helmstaedt, Kerstin"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Braus-Stromeyer, Susanna A."],["dc.contributor.author","Kwon, Nak-Jung"],["dc.contributor.author","Keller, Nancy P."],["dc.contributor.author","Yu, Jae-Hyuk"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2018-11-07T11:14:00Z"],["dc.date.available","2018-11-07T11:14:00Z"],["dc.date.issued","2008"],["dc.description.abstract","Differentiation and secondary metabolism are correlated processes in fungi that respond to light. In Aspergillus nidulans, light inhibits sexual reproduction as well as secondary metabolism. We identified the heterotrimeric velvet complex VelB/VeA/LaeA connecting light-responding developmental regulation and control of secondary metabolism. VeA, which is primarily expressed in the dark, physically interacts with VelB, which is expressed during sexual development. VeA bridges VelB to the nuclear master regulator of secondary metabolism, LaeA. Deletion of either velB or veA results in defects in both sexual fruiting-body formation and the production of secondary metabolites."],["dc.identifier.doi","10.1126/science.1155888"],["dc.identifier.isi","000256676400047"],["dc.identifier.pmid","18556559"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/54029"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Advancement Science"],["dc.relation.issn","0036-8075"],["dc.title","VelB/VeA/LaeA complex coordinates light signal with fungal development and secondary metabolism"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]