Braus, Gerhard H.

[["dc.bibliographiccitation.artnumber","e1007511"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","PLOS Genetics"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Thieme, Karl G."],["dc.contributor.author","Gerke, Jennifer"],["dc.contributor.author","Sasse, Christoph"],["dc.contributor.author","Valerius, Oliver"],["dc.contributor.author","Thieme, Sabine"],["dc.contributor.author","Karimi, Razieh"],["dc.contributor.author","Heinrich, Antje K."],["dc.contributor.author","Finkernagel, Florian"],["dc.contributor.author","Smith, Kristina"],["dc.contributor.author","Bode, Helge B."],["dc.contributor.author","Freitag, Michael"],["dc.contributor.author","Ram, Arthur F. J."],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2019-07-09T11:45:46Z"],["dc.date.available","2019-07-09T11:45:46Z"],["dc.date.issued","2018"],["dc.description.abstract","The NF-κB-like velvet domain protein VosA (viability of spores) binds to more than 1,500 promoter sequences in the filamentous fungus Aspergillus nidulans. VosA inhibits premature induction of the developmental activator gene brlA, which promotes asexual spore formation in response to environmental cues as light. VosA represses a novel genetic network controlled by the sclB gene. SclB function is antagonistic to VosA, because it induces the expression of early activator genes of asexual differentiation as flbC and flbD as well as brlA. The SclB controlled network promotes asexual development and spore viability, but is independent of the fungal light control. SclB interactions with the RcoA transcriptional repressor subunit suggest additional inhibitory functions on transcription. SclB links asexual spore formation to the synthesis of secondary metabolites including emericellamides, austinol as well as dehydroaustinol and activates the oxidative stress response of the fungus. The fungal VosA-SclB regulatory system of transcription includes a VosA control of the sclB promoter, common and opposite VosA and SclB control functions of fungal development and several additional regulatory genes. The relationship between VosA and SclB illustrates the presence of a convoluted surveillance apparatus of transcriptional control, which is required for accurate fungal development and the linkage to the appropriate secondary metabolism."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2018"],["dc.identifier.doi","10.1371/journal.pgen.1007511"],["dc.identifier.pmid","30044771"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15315"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59309"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1553-7404"],["dc.rights","CC BY 4.0"],["dc.rights.uri","http://creativecommons.org/licenses/by/4.0/"],["dc.subject.ddc","570"],["dc.title","Velvet domain protein VosA represses the zinc cluster transcription factor SclB regulatory network for Aspergillus nidulans asexual development, oxidative stress response and secondary metabolism."],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","744"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","9"],["dc.contributor.author","Bakti, Fruzsina"],["dc.contributor.author","Sasse, Christoph"],["dc.contributor.author","Heinekamp, Thorsten"],["dc.contributor.author","Pócsi, István"],["dc.contributor.author","Braus, Gerhard H."],["dc.date.accessioned","2019-07-09T11:45:38Z"],["dc.date.available","2019-07-09T11:45:38Z"],["dc.date.issued","2018"],["dc.description.abstract","Most of the metal transporters in Aspergillus fumigatus are yet uncharacterized. Their role in fungal metabolism and virulence remains unclear. This paper describes the novel PIB-type cation ATPase PcaA, which links metal homeostasis and heavy metal tolerance in the opportunistic human pathogen A. fumigatus. The protein possesses conserved ATPase motif and shares 51% amino acid sequence identity with the Saccharomyces cerevisiae cadmium exporter Pca1p. A pcaA deletion, an overexpression and a gfp-pcaA complementation strain of A. fumigatus were constructed and their heavy metal susceptibilities were studied. The pcaA knock out strain showed drastically decreased cadmium tolerance, however, its growth was not affected by the exposure to high concentrations of copper, iron, zinc, or silver ions. Although the lack of PcaA had no effect on copper adaption, we demonstrated that not only cadmium but also copper ions are able to induce the transcription of pcaA in A. fumigatus wild type Af293. Similarly, cadmium and copper ions could induce the copper exporting ATPase crpA. These data imply a general response on the transcriptomic level to heavy metals in A. fumigatus through the induction of detoxification systems. Confocal microscopy of the gfp-pcaA complementation strain expressing functional GFP-PcaA supports the predicted membrane localization of PcaA. The GFP-PcaA fusion protein is located in the plasma membrane of A. fumigatus in the presence of cadmium ions. Virulence assays support a function of PcaA for virulence of A. fumigatus in the Galleria mellonella wax moth larvae model, which might be linked to the elimination of reactive oxygen species."],["dc.identifier.doi","10.3389/fmicb.2018.00744"],["dc.identifier.pmid","29706948"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/15264"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/59270"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.subject.ddc","570"],["dc.title","Heavy Metal-Induced Expression of PcaA Provides Cadmium Tolerance to Aspergillus fumigatus and Supports Its Virulence in the Galleria mellonella Model"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]