Droese, Manfred

[["dc.bibliographiccitation.firstpage","219"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Archives of Dermatological Research"],["dc.bibliographiccitation.lastpage","225"],["dc.bibliographiccitation.volume","293"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Viehover, M."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Droese, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.date.accessioned","2018-11-07T09:05:20Z"],["dc.date.available","2018-11-07T09:05:20Z"],["dc.date.issued","2001"],["dc.description.abstract","Defects in DNA mismatch repair genes MLH1 and MSH2, first described in hereditary nonpolyposis colon cancer (HNPCC), have been postulated to be responsible for malignant transformation in several tumours. To date there are no data on cutaneous tumours, Using a PCR assay it was possible to identify deletions in MSH2 (exonic regions 12 and 13) in 16 of 47 lentigos maligna and in 10 of 36 malignant melanomas, Deletions in MLH1 (exonic regions 15 and 16) were found in 11 of 47 lentigos and in 15 of 36 melanomas. Comparison of DNA ploidy-related parameters between Lentigos with and without exonic deletions in MSH2 and MLH1 did not show any significant differences. In contrast, melanomas positive and negative for exons 12 and 13 (MSH2) (26/36 and 10/36, respectively) differed significantly with respect to the percentages of diploid cells (P = 0.0179) and tetraploid cells (P = 0.0042). Comparison of melanomas positive and negative for exons 15 and 16 (MLH1) (21/36 and 15/36, respectively) showed significant differences in the percentage of aneuploid cells between 2c and 4c (P = 0.0141) and tetraploid cells (P = 0.0404), In summary, deletions in DNA mismatch repair proteins MSH2 and MLH1 were present both in lentigo maligna and in melanomas and correlated with DNA ploidy; related parameters in malignant melanomas."],["dc.identifier.doi","10.1007/s004030100220"],["dc.identifier.isi","000169038500001"],["dc.identifier.pmid","11409565"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25293"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0340-3696"],["dc.title","Relationship between DNA ploidy-related parameters and the deletions in mismatch repair genes MLH1 and MSH2 in lentigo maligna and malignant melanomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","537"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Melanoma Research"],["dc.bibliographiccitation.lastpage","544"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Kotthaus, I."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:48:46Z"],["dc.date.available","2018-11-07T10:48:46Z"],["dc.date.issued","2000"],["dc.description.abstract","The main goal of this study was to examine the expression of DNA mismatch repair genes (MLH1, MSH2, PMS1 and PMS2), the adenomatous polyposis coli (APC) gene and growth arrest DNA damage inducible (GADD) genes (GADD34, GADD45 and GADD153) in the different stages of melanoma recurrences and metastases, and to identify any mutual consistencies in their expression pattern. All the cases of primary melanoma examined showed a reduced expression of DNA repair genes. These results demonstrate that disturbances of DNA repair begin in the early stages of melanoma. No significant differences were found in the expression of these markers between cutaneous melanomas and their recurrences and metastases (P > 0.05). Eighteen significant correlations between markers were found in the primary melanomas, and 10 significant correlations were observed in the first recurrences of melanoma. In contrast, 27 statistically significant relationships were demonstrated in metastatic lymph nodes. The different correlations found in primary and metastatic tumours confirmed the hypothetical difference in marker interaction in the diagnostic groups investigated. Our results suggest that DNA repair genes may play an important role in the recurrence and metastasis of melanomas. (C) 2000 Lippincott Williams & Wilkins."],["dc.identifier.doi","10.1097/00008390-200012000-00005"],["dc.identifier.isi","000165939900005"],["dc.identifier.pmid","11198475"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48275"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0960-8931"],["dc.title","Comparative study of the expression of DNA mismatch repair genes, the adenomatous polyposis coli gene and growth arrest DNA damage genes in melanoma recurrences and metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4499"],["dc.bibliographiccitation.issue","6B"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","4505"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Dengler, H."],["dc.contributor.author","Stachura, J."],["dc.contributor.author","Schauer, A."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T11:07:14Z"],["dc.date.available","2018-11-07T11:07:14Z"],["dc.date.issued","2000"],["dc.description.abstract","The importance of properly functioning DNA mismatch repair has been shown in several tumour types both hereditary and sporadic but not yet in malignant melanomas. The aim of this study was to examine the expression of DNA mismatch repair genes (MLH1, MSH2, PMS1 and PMS2) in primary melanomas and to define their possible prognostic impact in 106 primary melanomas. MLH1 was found in 64 and MSH2 in 61 out of 106 melanomas. PMS1 and PMS2 proteins were present in 69 and 67 tumours, respectively. Loss of the expression of DNA mismatch repair proteins correlated with the increase of Clark levels. Cox regression analysis demonstrated some prognostic significance for PMS1 (forward p = 0.0018 and backward selections p=0.0277), MLH1 (only forward selection p =0.0081) and MSH2 (only backward selection p =0.0115)."],["dc.identifier.isi","000166648300058"],["dc.identifier.pmid","11205295"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52511"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Analysis of the DNA mismatch repair proteins expression in malignant melanomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1781"],["dc.bibliographiccitation.issue","3A"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1785"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Middel, Peter"],["dc.contributor.author","Brinkman, U."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Radzun, H.-J."],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:52:16Z"],["dc.date.available","2018-11-07T10:52:16Z"],["dc.date.issued","2000"],["dc.description.abstract","Proliferative compartments of a tumour can be determined cytophotometrically, by in situ hybridisation or by immunohistochemical detection of Ki67 antigen. The main objective of this study was to analyse the proliferative activity during the progression of pigmented skin lesions with respect to differential diagnostic and prognostic applications. The material investigated consisted of 209 pigmented skin lesions (31 naevi, 30 dysplastic naevi, 106 primary melanomas, 20 lymphatic and 22 organ melanoma metastases). Comparison of the ratios of cells in the S-phase gained by two different methods (cytometry, in situ hybridisation) did not show any significant differences. The correlations between Ki67 and S-phase indices in every diagnostic group were highly significant. The results of forward and backward Cox regression were identical and only Ki67 showed an independent prognostic influence (p<0.001, coefficient in regression 0.02) with change in risk 2% and confidence limit ranging between 1.1% and 2.9%."],["dc.identifier.isi","000089222300068"],["dc.identifier.pmid","10928107"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49074"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Proliferative activity in the progression of pigmented skin lesions, diagnostic and prognostic significance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2791"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","2794"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Kotthaus, I."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:40:17Z"],["dc.date.available","2018-11-07T10:40:17Z"],["dc.date.issued","2000"],["dc.description.abstract","Ploidy status and ploidy minted parameters of 18 primary melanomas, 32 recurrences and 18 lymphatic metastases were investigated applying CAS200 image analyzer All the tumours investigated were either suspicious for aneuploidy (Auer III) or clearly aneuploid (Auer IV). Primary melanomas differed from recurrent tumours concerning the percentage of aneuploid cells between 4c and 8c and 5c ER. Comparison of cutaneous tumors with lymphatic metastases showed a significant difference concerning the percentage of aneuploid cells between 2c and 4c. An already high aneuploidy rate in primary rumours suggests that recurrent and metastatic clones of cells are present in early stages and that aneuploidy status in primary melanomas could be regarded as one of the risk factors of recurrences and metastases."],["dc.identifier.isi","000088559700078"],["dc.identifier.pmid","10953359"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/46267"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Analysis of the DNA content in the progression of recurrent and metastatic melanomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1685"],["dc.bibliographiccitation.issue","3A"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1690"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Brinkmann, U."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:52:08Z"],["dc.date.available","2018-11-07T10:52:08Z"],["dc.date.issued","2000"],["dc.description.abstract","5c exceeding rate is the parameter; most frequently showing prognostic impact. The CAS200 image analyzer makes possible the measurement of additional parameters defining single subfractions of cells, as for example the ratios of diploid, aneuploid, tetraploid, octaploid and 16-ploid cells. The main objective of this study was to define the prognostic significance of these new parameters in 106 primary melanomas with known survival time. 29 out of 106 melanomas were euploid. 77 out of 106 showed an aneuploid histogram. Multivariate analysis with Cox regression demonstrated that the percentage of aneuploid cells between 2c and 4c and the percentage of aneuploid cells between 4c and Sc, but not 5c exceeding I-ate, were able to influence survival time."],["dc.identifier.isi","000089222300053"],["dc.identifier.pmid","10928092"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49050"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Prognostic significance of newly defined ploidy related parameters in melanoma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","157"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Diagnostic Cytopathology"],["dc.bibliographiccitation.lastpage","162"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Kubitz, A."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T09:18:55Z"],["dc.date.available","2018-11-07T09:18:55Z"],["dc.date.issued","2001"],["dc.description.abstract","DNA-mismatch repair is essential for preventing genetic instability, and its important protective role has been demonstrated in several tumors. The main aim of this study was to investigate the expression of MLH1 and MSH2 (on the RNA level) in melanoma liver and lymph node metastases, and to define the relation between DNA ploidy status and mismatch repair gene expression. MLH1 was found in 29/33 melanoma lymph node and in 5/17 melanoma liver metastases. MSH2 was present in 26/33 lymph node and 5/17 liver metastases. A comparison of MLH1 and MSH2 positive and negative melanoma metastases showed that there were highly significant differences in the percentages of diploid cells, aneuploid cells between 4c and 8c, octaploid cells, and 5c exceeding rate. This fact confirms the strong relation between the loss of DNA-mismatch repair gene expression and advanced DNA aneuploidy status in melanoma metastases. (C) 2001 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/1097-0339(200103)24:3<157::AID-DC1033>3.0.CO;2-A"],["dc.identifier.isi","000167269600002"],["dc.identifier.pmid","11241897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28516"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","8755-1039"],["dc.title","Relation between DNA ploidy status and the expression of the DNA-mismatch repair genes MLH1 and MSH2 in cytological specimens of melanoma lymph node and liver metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]