Droese, Manfred

[["dc.bibliographiccitation.firstpage","219"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Archives of Dermatological Research"],["dc.bibliographiccitation.lastpage","225"],["dc.bibliographiccitation.volume","293"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Viehover, M."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Droese, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.date.accessioned","2018-11-07T09:05:20Z"],["dc.date.available","2018-11-07T09:05:20Z"],["dc.date.issued","2001"],["dc.description.abstract","Defects in DNA mismatch repair genes MLH1 and MSH2, first described in hereditary nonpolyposis colon cancer (HNPCC), have been postulated to be responsible for malignant transformation in several tumours. To date there are no data on cutaneous tumours, Using a PCR assay it was possible to identify deletions in MSH2 (exonic regions 12 and 13) in 16 of 47 lentigos maligna and in 10 of 36 malignant melanomas, Deletions in MLH1 (exonic regions 15 and 16) were found in 11 of 47 lentigos and in 15 of 36 melanomas. Comparison of DNA ploidy-related parameters between Lentigos with and without exonic deletions in MSH2 and MLH1 did not show any significant differences. In contrast, melanomas positive and negative for exons 12 and 13 (MSH2) (26/36 and 10/36, respectively) differed significantly with respect to the percentages of diploid cells (P = 0.0179) and tetraploid cells (P = 0.0042). Comparison of melanomas positive and negative for exons 15 and 16 (MLH1) (21/36 and 15/36, respectively) showed significant differences in the percentage of aneuploid cells between 2c and 4c (P = 0.0141) and tetraploid cells (P = 0.0404), In summary, deletions in DNA mismatch repair proteins MSH2 and MLH1 were present both in lentigo maligna and in melanomas and correlated with DNA ploidy; related parameters in malignant melanomas."],["dc.identifier.doi","10.1007/s004030100220"],["dc.identifier.isi","000169038500001"],["dc.identifier.pmid","11409565"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25293"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0340-3696"],["dc.title","Relationship between DNA ploidy-related parameters and the deletions in mismatch repair genes MLH1 and MSH2 in lentigo maligna and malignant melanomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","60"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Diagnostic Molecular Pathology"],["dc.bibliographiccitation.lastpage","65"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Nagel, H."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Schulz, G. M."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T09:18:58Z"],["dc.date.available","2018-11-07T09:18:58Z"],["dc.date.issued","2001"],["dc.description.abstract","Diagnostic accuracy in effusion cytology based on morphologic examination is not always satisfactory. Therefore, various diagnostic adjuncts such as immunocytochemistry or deoxyribonucleic acid cytometry are employed in this diagnostic field. Recently, demonstration of telomerase activity has been proposed as a possible marker for malignancy. In this study a seminested reverse transcription-polymerase chain reaction (RT-PCR) strategy for expression analysis of the catalytic subunit of human telomerase (hEST2) was used in 58 serous effusions. RT-PCR results correlated with cytologic diagnoses in 14 of 17 malignant effusions. In eight effusions cytologically suspicious for malignancy, PCR results were in accordance with the clinical follow-up. However, hEST2 RT-PCR was also positive in six of 15 cytologically benign effusions that consisted predominantly of inflammatory and mesothelial cells. Using the telomeric repeat amplification protocol, it could be demonstrated that cultured, proliferating benign mesothelial cells may present a weak telomerase activity, as is known in other benign cells including activated lymphocytes. In conclusion, the simple and rapid method of hEST2 RT-PCR serves to support the cytologic diagnosis of malignancy, but false-positive PCR results resulting from activated lymphocytes and proliferating mesothelial cells must be considered."],["dc.identifier.doi","10.1097/00019606-200103000-00010"],["dc.identifier.isi","000167166200010"],["dc.identifier.pmid","11277397"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28526"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.eventlocation","JENA, GERMANY"],["dc.relation.issn","1052-9551"],["dc.title","Gene expression analysis of the catalytic subunit of human telomerase (hEST2) in the differential diagnosis of serous effusions"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1545"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1551"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Thasler, W."],["dc.contributor.author","Gorzel, C."],["dc.contributor.author","Pahernik, S."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:29:59Z"],["dc.date.available","2018-11-07T10:29:59Z"],["dc.date.issued","2002"],["dc.description.abstract","Background: Preneoplastic and neoplastic lesions of the liver are suspected to arise as a result of estrogen treatment. Here we present the first report on the modulational effects of the steroids 17beta-estradiol (E2) and 17alpha-ethinylestradiol (EE2) on oncogene MDM2 in human hepatocytes. Materials and Methods:. Collagen-embedded cultures of hepatocytes stimulated with different E2/EE2 concentrations were analyzed by immunocytoehemistry, RT-PCR and sequencing for MDM2 protein/ mRNA expression, MDM2 mRNA splicing and MDM2 gene mutation. Results: The hepatocytes responded to stimulation with steroid E2/EE2 concentrations from 1-100 nmol/l with the overexpression of MDM2 protein while non-stimulated cells were negative. Stimulation with 1 nmol/l E2 and 10-100 nmol/l EE2 induced MDM2 splicing variants. Hepatocytes treated with 100 nmol/l E2 contained full-length MDM2 mRNA carrying a new type of MDM2 gene mutation. Unstimulated hepatocytes revealed neither mRNA splicing nor alteration of the MDM2 genes. Conclusion: The data show that steroid hormones are involved in the induction of MDM2 alterations in benign human hepatocytes. We speculate that some of the alterations may influence MDM2 function, thus possibly favouring genesis of liver changes."],["dc.identifier.isi","000177246500026"],["dc.identifier.pmid","12168835"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43763"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Detection of MDM2 alterations in cultured human hepatocytes treated with 17 beta-estradiol or 17 alpha-ethinylestradiol"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","67"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Pathobiology"],["dc.bibliographiccitation.lastpage","76"],["dc.bibliographiccitation.volume","69"],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Nagel, H."],["dc.contributor.author","Laskawi, Rainer"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T09:37:17Z"],["dc.date.available","2018-11-07T09:37:17Z"],["dc.date.issued","2001"],["dc.description.abstract","Introduction: Genetic alterations of oncogene MDM2 promote malignant transformation of several human tumors. In tumors of the salivary gland, however, the genetic status of MDM2 has not been evaluated so far. Methods and Results: Benign and malignant tumors of the salivary gland (6 pleomorphic adenomas, 3 Warthin's tumors, 1 adenocarcinoma, 1 basal cell adenocarcinoma, 1 mucoepidermoid carcinoma, 3 acinic cell carcinomas, 2 adenoid cystic carcinoma, 1 squamous cell carcinoma) were analyzed by fluorescence-based PCR techniques and immunochemistry for MDM2 gene amplification, MDM2 gene expression, MDM2 gene mutation, MDM2 RNA splicing and MDM2 accumulation. Data show that all samples contained nonamplified MDM2 genes with nonmutant zinc finger regions. However, in two benign and two malignant samples, novel MDM2 mRNA splicing variant types 1 and 2 were detected. Furthermore, three malignant tumors revealed significant nuclear MDM2 accumulation. Correlation between levels of MDM2 mRNA and MDM2 protein could not be detected in the specimens. Conclusion: The present study suggests that MDM2 gene mutation and gene amplification do not contribute to MDM2 accumulation detected in malignant tumors of the salivary gland. However, the role of novel MDM2 splicing variants in MDM2 expression and malignant transformation must be elucidated further. Copyright (C) 2001 S. Karger AG, Basel."],["dc.identifier.doi","10.1159/000048759"],["dc.identifier.isi","000173140900002"],["dc.identifier.pmid","11752900"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32807"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1015-2008"],["dc.title","Genetic analysis of the human oncoprotein MDM2 in benign and malignant tumors of the salivary gland"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1727"],["dc.bibliographiccitation.issue","3A"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1732"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Middel, Peter"],["dc.contributor.author","Laskawi, Rainer"],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:52:12Z"],["dc.date.available","2018-11-07T10:52:12Z"],["dc.date.issued","2000"],["dc.description.abstract","GAGE-1/-2 proteins are novel tumour markers, functionally related to tumour rejection. The objective of the present study was to identify the existence of a relationship between GAGE-1/-2 expression, Epstein Barr Virus (EBV) infection and viral infection-induced cytokine expression in cultivated tumour cells and archival specimens of undifferentiated carcinoma of nasopharyngeal type (UCNT). PCR and in situ hybridization techniques were employed. In cultivated UCNT cells, interferon-gamma (IFN-gamma) induced synthesis of GAGE-1/-2 mRNA. In archival tumour specimens (n=10) however; GAGE-1/-2 gene expression was detected in only 3/8 cases with coincident EBV infection and IFN-gamma expression. In conclusion, EBV infection appears to induce IFN-gamma gene expression in most tumors, but GAGE-1/-2 expression in only some tumours. The role of IFN-gamma and other factors in triggering GAGE-1/-2 gene activation must be elucidated further. The relevance of GAGE-1/-2 gene expression and its detection by PCR for future immunotherapy is discussed."],["dc.identifier.isi","000089222300061"],["dc.identifier.pmid","10928100"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49062"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Relationship between GAGE-1/-2 expression, EBV infection and interferon-gamma expression in undifferentiated carcinoma of nasopharyngeal type"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","157"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Diagnostic Cytopathology"],["dc.bibliographiccitation.lastpage","162"],["dc.bibliographiccitation.volume","24"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Kubitz, A."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T09:18:55Z"],["dc.date.available","2018-11-07T09:18:55Z"],["dc.date.issued","2001"],["dc.description.abstract","DNA-mismatch repair is essential for preventing genetic instability, and its important protective role has been demonstrated in several tumors. The main aim of this study was to investigate the expression of MLH1 and MSH2 (on the RNA level) in melanoma liver and lymph node metastases, and to define the relation between DNA ploidy status and mismatch repair gene expression. MLH1 was found in 29/33 melanoma lymph node and in 5/17 melanoma liver metastases. MSH2 was present in 26/33 lymph node and 5/17 liver metastases. A comparison of MLH1 and MSH2 positive and negative melanoma metastases showed that there were highly significant differences in the percentages of diploid cells, aneuploid cells between 4c and 8c, octaploid cells, and 5c exceeding rate. This fact confirms the strong relation between the loss of DNA-mismatch repair gene expression and advanced DNA aneuploidy status in melanoma metastases. (C) 2001 Wiley-Liss, Inc."],["dc.identifier.doi","10.1002/1097-0339(200103)24:3<157::AID-DC1033>3.0.CO;2-A"],["dc.identifier.isi","000167269600002"],["dc.identifier.pmid","11241897"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28516"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","8755-1039"],["dc.title","Relation between DNA ploidy status and the expression of the DNA-mismatch repair genes MLH1 and MSH2 in cytological specimens of melanoma lymph node and liver metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]