Droese, Manfred

[["dc.bibliographiccitation.firstpage","219"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Archives of Dermatological Research"],["dc.bibliographiccitation.lastpage","225"],["dc.bibliographiccitation.volume","293"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Viehover, M."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Droese, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.date.accessioned","2018-11-07T09:05:20Z"],["dc.date.available","2018-11-07T09:05:20Z"],["dc.date.issued","2001"],["dc.description.abstract","Defects in DNA mismatch repair genes MLH1 and MSH2, first described in hereditary nonpolyposis colon cancer (HNPCC), have been postulated to be responsible for malignant transformation in several tumours. To date there are no data on cutaneous tumours, Using a PCR assay it was possible to identify deletions in MSH2 (exonic regions 12 and 13) in 16 of 47 lentigos maligna and in 10 of 36 malignant melanomas, Deletions in MLH1 (exonic regions 15 and 16) were found in 11 of 47 lentigos and in 15 of 36 melanomas. Comparison of DNA ploidy-related parameters between Lentigos with and without exonic deletions in MSH2 and MLH1 did not show any significant differences. In contrast, melanomas positive and negative for exons 12 and 13 (MSH2) (26/36 and 10/36, respectively) differed significantly with respect to the percentages of diploid cells (P = 0.0179) and tetraploid cells (P = 0.0042). Comparison of melanomas positive and negative for exons 15 and 16 (MLH1) (21/36 and 15/36, respectively) showed significant differences in the percentage of aneuploid cells between 2c and 4c (P = 0.0141) and tetraploid cells (P = 0.0404), In summary, deletions in DNA mismatch repair proteins MSH2 and MLH1 were present both in lentigo maligna and in melanomas and correlated with DNA ploidy; related parameters in malignant melanomas."],["dc.identifier.doi","10.1007/s004030100220"],["dc.identifier.isi","000169038500001"],["dc.identifier.pmid","11409565"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/25293"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Springer"],["dc.relation.issn","0340-3696"],["dc.title","Relationship between DNA ploidy-related parameters and the deletions in mismatch repair genes MLH1 and MSH2 in lentigo maligna and malignant melanomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","351"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Diagnostic Cytopathology"],["dc.bibliographiccitation.lastpage","358"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Papaparaskeva, K."],["dc.contributor.author","Nagel, H."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:46:54Z"],["dc.date.available","2018-11-07T10:46:54Z"],["dc.date.issued","2000"],["dc.description.abstract","The cytomorphologic features in fine-needle aspiration (FNA) biopsies from 91 histologically verified medullary carcinomas of the thyroid (MCT) were investigated. FNA was able to diagnose neoplasms with indications of surgical removal in 98.9% of cases and moreover, was accurate in specific tumor typing in 89% of cases. The most important cytologic criteria of MCT with FNA are: dispersed cell-pattern of polygonal or triangular cells, azurophilic cytoplasmic granules, and extremely eccentrically placed nuclei with coarse granular chromatin and amyloid. These and other cytologic features of MCT are discussed in detail. Fourteen cases of thyroid tumors originally diagnosed as MCT by cytology are illustrated to discuss the differential diagnosis of MCT and its potential pitfalls. If MCT is cytologically presumed but amyloid and azurophilic cytoplasmic granules are not demonstrated, the use of immunostaining is necessary for a correct tumor typing. The application of immunocytochemistry in MCT is discussed. Diagn. Cytopathol. 2000;22:351-358. (C) 2000 Wiley-Liss, Inc."],["dc.identifier.isi","000087331700005"],["dc.identifier.pmid","10820528"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/47847"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","8755-1039"],["dc.title","Cytologic diagnosis of medullary carcinoma of the thyroid gland"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","60"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Diagnostic Molecular Pathology"],["dc.bibliographiccitation.lastpage","65"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Nagel, H."],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Schulz, G. M."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T09:18:58Z"],["dc.date.available","2018-11-07T09:18:58Z"],["dc.date.issued","2001"],["dc.description.abstract","Diagnostic accuracy in effusion cytology based on morphologic examination is not always satisfactory. Therefore, various diagnostic adjuncts such as immunocytochemistry or deoxyribonucleic acid cytometry are employed in this diagnostic field. Recently, demonstration of telomerase activity has been proposed as a possible marker for malignancy. In this study a seminested reverse transcription-polymerase chain reaction (RT-PCR) strategy for expression analysis of the catalytic subunit of human telomerase (hEST2) was used in 58 serous effusions. RT-PCR results correlated with cytologic diagnoses in 14 of 17 malignant effusions. In eight effusions cytologically suspicious for malignancy, PCR results were in accordance with the clinical follow-up. However, hEST2 RT-PCR was also positive in six of 15 cytologically benign effusions that consisted predominantly of inflammatory and mesothelial cells. Using the telomeric repeat amplification protocol, it could be demonstrated that cultured, proliferating benign mesothelial cells may present a weak telomerase activity, as is known in other benign cells including activated lymphocytes. In conclusion, the simple and rapid method of hEST2 RT-PCR serves to support the cytologic diagnosis of malignancy, but false-positive PCR results resulting from activated lymphocytes and proliferating mesothelial cells must be considered."],["dc.identifier.doi","10.1097/00019606-200103000-00010"],["dc.identifier.isi","000167166200010"],["dc.identifier.pmid","11277397"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/28526"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.publisher.place","Philadelphia"],["dc.relation.eventlocation","JENA, GERMANY"],["dc.relation.issn","1052-9551"],["dc.title","Gene expression analysis of the catalytic subunit of human telomerase (hEST2) in the differential diagnosis of serous effusions"],["dc.type","conference_paper"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","537"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Melanoma Research"],["dc.bibliographiccitation.lastpage","544"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Kotthaus, I."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:48:46Z"],["dc.date.available","2018-11-07T10:48:46Z"],["dc.date.issued","2000"],["dc.description.abstract","The main goal of this study was to examine the expression of DNA mismatch repair genes (MLH1, MSH2, PMS1 and PMS2), the adenomatous polyposis coli (APC) gene and growth arrest DNA damage inducible (GADD) genes (GADD34, GADD45 and GADD153) in the different stages of melanoma recurrences and metastases, and to identify any mutual consistencies in their expression pattern. All the cases of primary melanoma examined showed a reduced expression of DNA repair genes. These results demonstrate that disturbances of DNA repair begin in the early stages of melanoma. No significant differences were found in the expression of these markers between cutaneous melanomas and their recurrences and metastases (P > 0.05). Eighteen significant correlations between markers were found in the primary melanomas, and 10 significant correlations were observed in the first recurrences of melanoma. In contrast, 27 statistically significant relationships were demonstrated in metastatic lymph nodes. The different correlations found in primary and metastatic tumours confirmed the hypothetical difference in marker interaction in the diagnostic groups investigated. Our results suggest that DNA repair genes may play an important role in the recurrence and metastasis of melanomas. (C) 2000 Lippincott Williams & Wilkins."],["dc.identifier.doi","10.1097/00008390-200012000-00005"],["dc.identifier.isi","000165939900005"],["dc.identifier.pmid","11198475"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48275"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Lippincott Williams & Wilkins"],["dc.relation.issn","0960-8931"],["dc.title","Comparative study of the expression of DNA mismatch repair genes, the adenomatous polyposis coli gene and growth arrest DNA damage genes in melanoma recurrences and metastases"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1545"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1551"],["dc.bibliographiccitation.volume","22"],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Thasler, W."],["dc.contributor.author","Gorzel, C."],["dc.contributor.author","Pahernik, S."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:29:59Z"],["dc.date.available","2018-11-07T10:29:59Z"],["dc.date.issued","2002"],["dc.description.abstract","Background: Preneoplastic and neoplastic lesions of the liver are suspected to arise as a result of estrogen treatment. Here we present the first report on the modulational effects of the steroids 17beta-estradiol (E2) and 17alpha-ethinylestradiol (EE2) on oncogene MDM2 in human hepatocytes. Materials and Methods:. Collagen-embedded cultures of hepatocytes stimulated with different E2/EE2 concentrations were analyzed by immunocytoehemistry, RT-PCR and sequencing for MDM2 protein/ mRNA expression, MDM2 mRNA splicing and MDM2 gene mutation. Results: The hepatocytes responded to stimulation with steroid E2/EE2 concentrations from 1-100 nmol/l with the overexpression of MDM2 protein while non-stimulated cells were negative. Stimulation with 1 nmol/l E2 and 10-100 nmol/l EE2 induced MDM2 splicing variants. Hepatocytes treated with 100 nmol/l E2 contained full-length MDM2 mRNA carrying a new type of MDM2 gene mutation. Unstimulated hepatocytes revealed neither mRNA splicing nor alteration of the MDM2 genes. Conclusion: The data show that steroid hormones are involved in the induction of MDM2 alterations in benign human hepatocytes. We speculate that some of the alterations may influence MDM2 function, thus possibly favouring genesis of liver changes."],["dc.identifier.isi","000177246500026"],["dc.identifier.pmid","12168835"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43763"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Detection of MDM2 alterations in cultured human hepatocytes treated with 17 beta-estradiol or 17 alpha-ethinylestradiol"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","67"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","Pathobiology"],["dc.bibliographiccitation.lastpage","76"],["dc.bibliographiccitation.volume","69"],["dc.contributor.author","Schlott, T."],["dc.contributor.author","Nagel, H."],["dc.contributor.author","Laskawi, Rainer"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T09:37:17Z"],["dc.date.available","2018-11-07T09:37:17Z"],["dc.date.issued","2001"],["dc.description.abstract","Introduction: Genetic alterations of oncogene MDM2 promote malignant transformation of several human tumors. In tumors of the salivary gland, however, the genetic status of MDM2 has not been evaluated so far. Methods and Results: Benign and malignant tumors of the salivary gland (6 pleomorphic adenomas, 3 Warthin's tumors, 1 adenocarcinoma, 1 basal cell adenocarcinoma, 1 mucoepidermoid carcinoma, 3 acinic cell carcinomas, 2 adenoid cystic carcinoma, 1 squamous cell carcinoma) were analyzed by fluorescence-based PCR techniques and immunochemistry for MDM2 gene amplification, MDM2 gene expression, MDM2 gene mutation, MDM2 RNA splicing and MDM2 accumulation. Data show that all samples contained nonamplified MDM2 genes with nonmutant zinc finger regions. However, in two benign and two malignant samples, novel MDM2 mRNA splicing variant types 1 and 2 were detected. Furthermore, three malignant tumors revealed significant nuclear MDM2 accumulation. Correlation between levels of MDM2 mRNA and MDM2 protein could not be detected in the specimens. Conclusion: The present study suggests that MDM2 gene mutation and gene amplification do not contribute to MDM2 accumulation detected in malignant tumors of the salivary gland. However, the role of novel MDM2 splicing variants in MDM2 expression and malignant transformation must be elucidated further. Copyright (C) 2001 S. Karger AG, Basel."],["dc.identifier.doi","10.1159/000048759"],["dc.identifier.isi","000173140900002"],["dc.identifier.pmid","11752900"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32807"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Karger"],["dc.relation.issn","1015-2008"],["dc.title","Genetic analysis of the human oncoprotein MDM2 in benign and malignant tumors of the salivary gland"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","956"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Biochemical and Biophysical Research Communications"],["dc.bibliographiccitation.lastpage","963"],["dc.bibliographiccitation.volume","283"],["dc.contributor.author","Schlott, T"],["dc.contributor.author","Scharf, J. G."],["dc.contributor.author","Soruri, A"],["dc.contributor.author","Fayyazi, A"],["dc.contributor.author","Griesinger, Christian"],["dc.contributor.author","Albrecht, C."],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2017-09-07T11:46:07Z"],["dc.date.available","2017-09-07T11:46:07Z"],["dc.date.issued","2001"],["dc.description.abstract","Human oncoprotein MDM2 reveals a MHC class I binding motif HMDM441 characterizing MDM2 as a potential tumor antigen. To analyze the distribution of MDM2 proteins containing this motif in liver cancer cells we produced rabbit anti-HMDM441 serum. The novel antibodies bound to an MDM2 fragment of approximately 55 kDa which lacked the N-terminal region and was present in lysate and supernatant of a human hepatoma cell line overexpressing normal 90-kDa MDM2. The 55-kDa fragment was detected in the cytoplasm and nucleoli and at the nuclear envelope of hepatoma cells, whereas normal hepatocytes were negative. Double-fluorescence labeling indicated that the MDM2 fragments and MHC class I molecules were coexpressed on the surface of the hepatoma cells. Further studies must clarify whether MDM2 fragments containing motif HMDM441 are novel targets of immunotherapy and immunochemical tumor diagnosis. (C) 2001 Academic Press."],["dc.identifier.doi","10.1006/bbrc.2001.4868"],["dc.identifier.gro","3144284"],["dc.identifier.isi","000168928400037"],["dc.identifier.pmid","11350078"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1891"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","PUB_WoS_Import"],["dc.publisher","Academic Press Inc"],["dc.relation.issn","0006-291X"],["dc.title","Fragments of human oncoprotein MDM2 reveal variable distribution within and on cultivated human hepatoma cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4499"],["dc.bibliographiccitation.issue","6B"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","4505"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Dengler, H."],["dc.contributor.author","Stachura, J."],["dc.contributor.author","Schauer, A."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T11:07:14Z"],["dc.date.available","2018-11-07T11:07:14Z"],["dc.date.issued","2000"],["dc.description.abstract","The importance of properly functioning DNA mismatch repair has been shown in several tumour types both hereditary and sporadic but not yet in malignant melanomas. The aim of this study was to examine the expression of DNA mismatch repair genes (MLH1, MSH2, PMS1 and PMS2) in primary melanomas and to define their possible prognostic impact in 106 primary melanomas. MLH1 was found in 64 and MSH2 in 61 out of 106 melanomas. PMS1 and PMS2 proteins were present in 69 and 67 tumours, respectively. Loss of the expression of DNA mismatch repair proteins correlated with the increase of Clark levels. Cox regression analysis demonstrated some prognostic significance for PMS1 (forward p = 0.0018 and backward selections p=0.0277), MLH1 (only forward selection p =0.0081) and MSH2 (only backward selection p =0.0115)."],["dc.identifier.isi","000166648300058"],["dc.identifier.pmid","11205295"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52511"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Analysis of the DNA mismatch repair proteins expression in malignant melanomas"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1861"],["dc.bibliographiccitation.issue","3A"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1864"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Hofmann, M."],["dc.contributor.author","Diab, E."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:52:18Z"],["dc.date.available","2018-11-07T10:52:18Z"],["dc.date.issued","2000"],["dc.description.abstract","With regard to neoplasms of hepatocytes, diagnostic pitfalls have been reported for differentiation of live cell adenoma (LCA) from well differentiated hepatocellular carcinoma (HCC). Since cytophotometric analysis of the DNA content with the help of image analysis has proven to be of diagnostic value in various neoplasms, we examined ifs ability to discriminate between LCA and HCC as well as regenerative liver nodules. The material investigated consisted of 54 cases of HCC, 10 benign liver armours and 10 cases suspicious for HCC. All the benign liver tumours demonstrated an euploid histogram. 9 out of 10 borderline tumours were euploid while I out of 10 was suspiciously aneuploid Among HCC, 21 out of 54 were euploid, 18 out of 54 suspiciously and 15 out of 54 clearly aneuploid. 5c exceeding rate differed significantly between benign liver changes and borderline lesions (p=0.0474) as well as between borderline lesions and malignant tumours (p=0.0108). We conclude that the use of image cytometry is helpful as an additional criterion for more diagnostic accuracy in morphologically difficult cases."],["dc.identifier.isi","000089222300080"],["dc.identifier.pmid","10928119"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49086"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Comparison of the DNA content in liver cell adenoma, hepatocellular carcinoma and regenerative nodules"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1781"],["dc.bibliographiccitation.issue","3A"],["dc.bibliographiccitation.journal","Anticancer Research"],["dc.bibliographiccitation.lastpage","1785"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Korabiowska, M."],["dc.contributor.author","Brinck, Ulrich"],["dc.contributor.author","Middel, Peter"],["dc.contributor.author","Brinkman, U."],["dc.contributor.author","Berger, H."],["dc.contributor.author","Radzun, H.-J."],["dc.contributor.author","Ruschenburg, I."],["dc.contributor.author","Droese, M."],["dc.date.accessioned","2018-11-07T10:52:16Z"],["dc.date.available","2018-11-07T10:52:16Z"],["dc.date.issued","2000"],["dc.description.abstract","Proliferative compartments of a tumour can be determined cytophotometrically, by in situ hybridisation or by immunohistochemical detection of Ki67 antigen. The main objective of this study was to analyse the proliferative activity during the progression of pigmented skin lesions with respect to differential diagnostic and prognostic applications. The material investigated consisted of 209 pigmented skin lesions (31 naevi, 30 dysplastic naevi, 106 primary melanomas, 20 lymphatic and 22 organ melanoma metastases). Comparison of the ratios of cells in the S-phase gained by two different methods (cytometry, in situ hybridisation) did not show any significant differences. The correlations between Ki67 and S-phase indices in every diagnostic group were highly significant. The results of forward and backward Cox regression were identical and only Ki67 showed an independent prognostic influence (p<0.001, coefficient in regression 0.02) with change in risk 2% and confidence limit ranging between 1.1% and 2.9%."],["dc.identifier.isi","000089222300068"],["dc.identifier.pmid","10928107"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49074"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Int Inst Anticancer Research"],["dc.relation.issn","0250-7005"],["dc.title","Proliferative activity in the progression of pigmented skin lesions, diagnostic and prognostic significance"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]