Legler, Tobias J.

[["dc.bibliographiccitation.firstpage","109"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Clinical Apheresis"],["dc.bibliographiccitation.lastpage","113"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Humpe, Andreas"],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Koch, S."],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Munzel, U."],["dc.contributor.author","Kohler, M."],["dc.date.accessioned","2018-11-07T09:34:58Z"],["dc.date.available","2018-11-07T09:34:58Z"],["dc.date.issued","2001"],["dc.description.abstract","Some data exist on the influence of leukapheresis volume on the number of harvested peripheral blood hematopoietic progenitor cells (HPC), but less is known about the influence on the composition of HPC. We therefore performed a prospective, randomized crossover trial to evaluate the effect of large-volume (LVL) vs. normal-volume leukapheresis (NVL) on subpopulations of CD34(+) cells in the harvest product of 15 patients with breast cancer and 8 patients with non-Hodgkin's lymphoma. Patients were randomly assigned to start either with an LVL on day 1 followed by an NVL on day 2 or vice versa. The number of HPC; the extraction efficiency defined as difference between yield in the harvest and decrease in peripheral blood, and the relative proportion as well as the absolute numbers of CD34(+) cells coexpressing CD38, CD90, HLA-DR, CD117, CD7, CD19, CD41, or CD33 were evaluated. There was no significant difference with regard to the percentages of the subsets on comparison of LVL to NVL procedures. Only the absolute median number of CD34(+)HLA-DR- cells was significantly (P=0.02) higher in LVL harvests compared with the corresponding NVL components; which can be explained on the basis of the higher yield and the higher extraction efficiency in LVL compared with NVL. LVL results in a higher yield of CD34(+) cells and leads to an intra-apheresis recruitment of HPC but the relative composition of the harvested CD34(+) cells is not changed significantly. In addition, the amount of early, HLA-DR-, hematopoietic HPC seems to be increased by an LVL. (C) 2001 Wiley-Liss, Inc."],["dc.identifier.isi","000171650400001"],["dc.identifier.pmid","11746535"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/32289"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-liss"],["dc.relation.issn","0733-2459"],["dc.title","Prospective, randomized, sequential, crossover trial of large-volume vs. normal-volume leukapheresis procedures: Effects on subpopulations of CD34(+) cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","612"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Transfusion"],["dc.bibliographiccitation.lastpage","618"],["dc.bibliographiccitation.volume","42"],["dc.contributor.author","Lynen, R."],["dc.contributor.author","Krone, O."],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Kohler, M."],["dc.contributor.author","Mayr, Wolfgang R."],["dc.date.accessioned","2018-11-07T10:30:29Z"],["dc.date.available","2018-11-07T10:30:29Z"],["dc.date.issued","2002"],["dc.description.abstract","BACKGROUND: Novel gel centrifugation test (GCT) cards were evaluated with respect to their ability to estimate the quantity of IgG on RBCs and the determination of the IgG subclasses IgG1 and IgG3. STUDY DESIGN AND METHODS: In 65 patients with a positive DAT, the amount of IgG-gamma-, IgG1, and IgG3 on RBCs was examined by use of GCT cards and flow cytometry (FC) in parallel. The results were correlated with the presence or absence of hemolysis. In addition, D+ RBCs were studied after sensitization with anti-D sera from 22 alloimmunized pregnant women. RESULTS: The amount of IgG on the RBCs as determined by GCT dilution cards correlated with FC (r = 0.70, p < 0.0001). IgG subclass results as determined by GCT IgG subclass cards were confirmed by FC in 14 cases with an anti-IgG-gamma-chain titer greater than or equal to300, whereas IgG subclass cards were not suitable in cases with anti-IgG-gamma-chain titers less than 300. In 44 patients with 2+ or 3+ DAT in the GCT and anti-IgG-gamma-chain titer less than or equal to30, no hemolysis was observed, whereas hemolysis occurred in 13 of 14 patients with an anti-IgG-gamma-chain titer greater than or equal to300. GCT data obtained by IATs with anti-D sera were concordant with FC results. CONCLUSION: There is a correlation between the amount of RBC-bound IgG and immune hemolysis. The GCT cards that detect the anti-IgG-gamma-chain may be useful to predict hemolysis in patients with a 2+ or 3+ DAT in the GCT. The diagnostic value of GCT cards for IgG subclass testing should be investigated further."],["dc.identifier.doi","10.1046/j.1537-2995.2002.00076.x"],["dc.identifier.isi","000176104300014"],["dc.identifier.pmid","12084170"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/43882"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Blood Banks"],["dc.relation.issn","0041-1132"],["dc.title","A newly developed gel centrifugation test for quantification of RBC-bound IgG antibodies and their subclasses IgG1 and IgG3: comparison with flow cytometry"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","830"],["dc.bibliographiccitation.issue","6"],["dc.bibliographiccitation.journal","Transfusion"],["dc.bibliographiccitation.lastpage","831"],["dc.bibliographiccitation.volume","43"],["dc.contributor.author","Koehler, M."],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Mayr, Wolfgang R."],["dc.contributor.author","Schwartz, DWM"],["dc.contributor.author","Heermann, K. H."],["dc.date.accessioned","2018-11-07T10:38:50Z"],["dc.date.available","2018-11-07T10:38:50Z"],["dc.date.issued","2003"],["dc.identifier.doi","10.1046/j.1537-2995.2003.00409.x"],["dc.identifier.isi","000183119000025"],["dc.identifier.pmid","12757539"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/45903"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Blood Banks"],["dc.relation.issn","0041-1132"],["dc.title","Risk of transfusion-transmitted infections by NAT-negative blood"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.subtype","letter_note"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","571"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Transfusion"],["dc.bibliographiccitation.lastpage","574"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Wagner, T."],["dc.contributor.author","Resch, B."],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Mossier, C."],["dc.contributor.author","Helmberg, W."],["dc.contributor.author","Kohler, M."],["dc.contributor.author","Lanzer, G."],["dc.date.accessioned","2018-11-07T10:56:18Z"],["dc.date.available","2018-11-07T10:56:18Z"],["dc.date.issued","2000"],["dc.description.abstract","BACKGROUND: Rh system antibodies are commonly encountered in blood bank practice as well as during pregnancy. Nevertheless, no examples of anti-Ce (RH7) have been reported as a cause of HDN that requires exchange transfusion. CASE REPORT: A 38-year-old woman in her fourth pregnancy was typed as blood group O D+, C-, c+, E+, e-. Anti-C and anti-e were detected in her serum during a routine prenatal work-up. Further evaluation, including flow cytometric analysis, revealed the presence of a strong anti-Ce and a weak anti-e. Her partner was typed as group A D+, C+, c-, E-, e+. A seemingly healthy male infant was delivered at 40 weeks of gestation. The infant's RBCs were typed as group O D-, C+, c+, E+, e+ with a positive DAT (titer 128). Twenty-five hours after birth, the baby had to be transferred to the neonatal intensive care unit because of rapidly rising total serum bilirubin. Despite intensive treatment, including double phototherapy, albumin infusion, and the administration of furosemide and IVIG, the total serum bilirubin level increased during the following day and exchange transfusion with 2 units of type O D-, C-, c+, E+, e- had to be performed; this resulted in a prompt decrease in total serum bilirubin without relapse. CONCLUSION: Anti-Ce caused severe HDN requiring exchange transfusion. This highlights the need for a close follow-up throughout pregnancy if unexpected RBC antibodies are present, to permit the provision of compatible blood in case of a rare antibody."],["dc.identifier.doi","10.1046/j.1537-2995.2000.40050571.x"],["dc.identifier.isi","000087147300013"],["dc.identifier.pmid","10827261"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/49982"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Blood Banks"],["dc.relation.issn","0041-1132"],["dc.title","Severe HDN due to anti-Ce that required exchange tranfusion"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2292"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Transfusion"],["dc.bibliographiccitation.lastpage","2301"],["dc.bibliographiccitation.volume","48"],["dc.contributor.author","Mueller, Sina Patricia"],["dc.contributor.author","Bartels, Iris"],["dc.contributor.author","Stein, Werner"],["dc.contributor.author","Emons, Guenther"],["dc.contributor.author","Gutensohn, Kai"],["dc.contributor.author","Koehler, Michael"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.date.accessioned","2018-11-07T11:09:47Z"],["dc.date.available","2018-11-07T11:09:47Z"],["dc.date.issued","2008"],["dc.description.abstract","Noninvasive fetal RHD genotyping might become a valuable tool in decision making on antenatal Rh prophylaxis, which is currently in routine practice for all D- pregnancies in several countries. This study provides a large-scale validation study of this technology to address questions concerning feasibility and applicability of its introduction into clinical routine. Real-time polymerase chain reaction (PCR) targeting RHD Exons 5 and 7 was applied for the detection of fetal-specific RHD sequences in maternal plasma. A total of 1113 women in 6 to 32 weeks (median, Week 25) of pregnancy were recruited. All of them were serologically typed as D- according to current German guidelines. DNA was extracted via a spin-column method and a novel automated approach using magnetic tips. Real-time PCR results were compared with postnatal serology and discrepancies further elucidated by DNA sequencing from a newborn's buccal swab. Sensitivities of fetal RHD genotyping were 99.7 percent (spin columns) and 99.8 percent (magnetic tips), thus comparable with serology (99.5%). The detection of weak D variants was more reliable by real-time PCR. Specificities of fetal RHD genotyping were 99.2 percent (spin columns) and 98.1 percent (magnetic tips), which is lower than serology (> 99.7%). Automation achieved significantly higher yields of cell-free fetal DNA. This prospective clinical trial revealed that routine determination of the fetal D status from maternal plasma is feasible. Noninvasive fetal RHD genotyping can be considered as sensitive as the traditional postnatal serologic assay."],["dc.identifier.doi","10.1111/j.1537-2995.2008.01843.x"],["dc.identifier.isi","000261086200007"],["dc.identifier.pmid","18694461"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6289"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53084"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0041-1132"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The determination of the fetal D status from maternal plasma for decision making on Rh prophylaxis is feasible"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","383"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Transfusion Medicine"],["dc.bibliographiccitation.lastpage","388"],["dc.bibliographiccitation.volume","11"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Maas, J. H."],["dc.contributor.author","Kohler, M."],["dc.contributor.author","Wagner, T."],["dc.contributor.author","Daniels, G. L."],["dc.contributor.author","Perco, P."],["dc.contributor.author","Panzer, S."],["dc.date.accessioned","2018-11-07T08:38:07Z"],["dc.date.available","2018-11-07T08:38:07Z"],["dc.date.issued","2001"],["dc.description.abstract","The serological differentiation of weak D from partial D, D-negative and D-positive is not always unequivocal. Therefore, sequencing of the RHD gene is required in some cases. Very recently, several new differences between RHD and RHCE have been identified which permitted us to design primers close to the exon/intron boundaries of the RHD-exons. We evaluated these primers in 83 D-positive and 18 D-negative blood donors and applied the new method for the characterization of the RHD gene in six individuals with weak D phenotype. The amplification reactions were concordant with serological findings in 100 of 101 donors (99.0%). In one D-positive donor the PCR for exons 2 and 5 gave a negative result, while the sequence of the remaining eight exons was unchanged. By sequencing samples with very weak D serological reactions, we identified weak D type 4.2.2 and weak D type 15, both previously reported to be associated with anti-D-alloimmunization. Consequently, we recommended the selection of D-negative blood in the weak D type 4.2.2 patient, and the provision of Rh prophylaxis for pregnant women with weak D type 15. In summary, a new RHD sequencing method was developed which can be applied if serological reactions are inconclusive."],["dc.identifier.doi","10.1046/j.1365-3148.2001.00327.x"],["dc.identifier.isi","000171923400005"],["dc.identifier.pmid","11696232"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/18694"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Science Ltd"],["dc.relation.issn","0958-7578"],["dc.title","RHD sequencing: a new tool for decision making on transfusion therapy and provision of Rh prophylaxis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","263"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","Vox Sanguinis"],["dc.bibliographiccitation.lastpage","265"],["dc.bibliographiccitation.volume","86"],["dc.contributor.author","Schanz, J."],["dc.contributor.author","Wolf, C."],["dc.contributor.author","Koehler, M."],["dc.contributor.author","Maas, J. H."],["dc.contributor.author","Meyer, M."],["dc.contributor.author","Neumeyer, H."],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Wulf, Gerald"],["dc.contributor.author","Glass, Bertram"],["dc.contributor.author","Truemper, Lorenz H."],["dc.contributor.author","Riggert, Joachim"],["dc.date.accessioned","2018-11-07T10:49:32Z"],["dc.date.available","2018-11-07T10:49:32Z"],["dc.date.issued","2004"],["dc.identifier.doi","10.1111/j.0042-9007.2004.00486.x"],["dc.identifier.isi","000221402500007"],["dc.identifier.pmid","15144532"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/48455"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing Ltd"],["dc.relation.issn","0042-9007"],["dc.title","Rhabdomyolysis in allogeneic peripheral blood stem cell donors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","784"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Thrombosis and Haemostasis"],["dc.bibliographiccitation.lastpage","788"],["dc.bibliographiccitation.volume","84"],["dc.contributor.author","Humpe, Andreas"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Nubling, C. M."],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Unger, G."],["dc.contributor.author","Wolf, C."],["dc.contributor.author","Heermann, K. H."],["dc.contributor.author","Kohler, M."],["dc.date.accessioned","2018-11-07T08:54:09Z"],["dc.date.available","2018-11-07T08:54:09Z"],["dc.date.issued","2000"],["dc.description.abstract","In 1994, quarantine fresh-frozen plasma (Q-FFP) was introduced in Germany in order to reduce the risk of HIV and HCV transmission In 1998, an acute HCV infection of a patient was reported to us. The look-back revealed that this patient had received two Q-FFP from a donor who had seroconverted for HCV in the meantime. Recipients of further plasma donations from this donor were identified. Back-up specimens of these donations were investigated in several laboratories. A total of 25 additional HCV-PCR positive plasma units had been transfused to 12 further patients. HCV infections were diagnosed in seven of these recipients, three patients had already been deceased. One of the remaining two recipients was already HCV positive prior to transfusion, in the other patient, no HCV infection was detectable. This patient had received three units of an \"early\" plasma donation, which was tested negative by PCR in one laboratory, but positive in the other. The subsequent, clinically infectious donation had the same discrepant PCR results. Thus, eight cases of HCV transmission were revealed and classified as \"certain\" with regard to causality, also due to an identical HCV genotype, i.e. 3e. Some of these infections would have been prevented by application of a different anti-HCV assay. The assay used in the respective plasmapheresis station was in-sensitive in this individual case for more than 400 days after the first PCR positive donation. This caused the release of the above mentioned infectious units. Upon re-testing the backups, three of four other anti-HCV assays revealed a positive result already 104 days after the first PCR-positive donation. The donor had increased ALAT levels (> 23 IU/L) at nine of 28 donations, two of these were higher than 2.5 times the upper normal limit, and two were higher than 68 IU/L, which is the cut-off value for male blood donors in Germany. The results of these (look-back) studies arouse several queries, i.e, differences in the diagnostic sensitivity between current anti-HCV and PCR tests, the accuracy of risk-estimates (especially when based on hemovigilance studies for Q-FFP), the value of ALAT testing, and currently practised release algorithms for Q-FFP."],["dc.identifier.isi","000165403900010"],["dc.identifier.pmid","11127856"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22607"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","F K Schattauer Verlag Gmbh"],["dc.relation.issn","0340-6245"],["dc.title","Hepatitis C virus transmission through quarantine fresh-frozen plasma"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1192"],["dc.bibliographiccitation.issue","10"],["dc.bibliographiccitation.journal","Transfusion"],["dc.bibliographiccitation.lastpage","1197"],["dc.bibliographiccitation.volume","40"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.contributor.author","Riggert, Joachim"],["dc.contributor.author","Simson, G."],["dc.contributor.author","Wolf, C."],["dc.contributor.author","Humpe, Andreas"],["dc.contributor.author","Munzel, U."],["dc.contributor.author","Uy, Angela"],["dc.contributor.author","Kohler, M."],["dc.contributor.author","Heermann, K. H."],["dc.date.accessioned","2018-11-07T10:01:05Z"],["dc.date.available","2018-11-07T10:01:05Z"],["dc.date.issued","2000"],["dc.description.abstract","BACKGROUND: To allow cost-effective RNA testing with NAT techniques, the national authorities of several countries have planned or already introduced tests of mixed specimens, that is, plasma pools. STUDY DESIGN AND METHODS: High-throughput extraction, amplification, and detection of HCV RNA from individual blood donations were optimized and validated. The feasibility of the method and the frequency of anti-HCV-negative, HCV RNA-positive donations were determined in a prospective study of 27,745 allogeneic and 792 autologous individual donations. RESULTS: The 50- and 95-percent detection limits of the method were determined at 44 IU per mt and 162 IU per mt, respectively (World Health Organization HCV reference material). When 201 HCV RNA-positive sera were taken as a reference, the sensitivity was 97.5 percent. The assay specificity was determined at 99.77 percent. During a 20-month period, two seronegative blood donors tested positive in HCV PCR. The viral load of these donations was 6 x 10(6) and 3 x 10(7) copies per mL, respectively. Thus, the yield of HCV RNA testing in this study was 7.63 per 100,000 screened donations (95% CI, 1.25-22.07). In both PCR-positive donors, seroconversion was found in subsequent blood samples. CONCLUSION: This study compares the feasibility of single-donation HCV RNA screening, with the detection of a relatively high percentage of window-phase donations, to data reported from groups using HCV RNA testing of plasma pools. The relative yield of NAT of individual donations versus minipools should be directly investigated in the near future."],["dc.identifier.doi","10.1046/j.1537-2995.2000.40101192.x"],["dc.identifier.isi","000089844200009"],["dc.identifier.pmid","11061854"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/37942"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Assoc Blood Banks"],["dc.relation.issn","0041-1132"],["dc.title","Testing of individual blood donations for HCV RNA reduces the residual risk of transfusion-transmitted HCV infection"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","PII S1473-0502(02)00068-X"],["dc.bibliographiccitation.firstpage","217"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Transfusion and Apheresis Science"],["dc.bibliographiccitation.lastpage","223"],["dc.bibliographiccitation.volume","27"],["dc.contributor.author","Lynen, Rainer"],["dc.contributor.author","Maas, Jens-Holger"],["dc.contributor.author","Pindur, Gerhard"],["dc.contributor.author","Kulenkampff, Dietrich"],["dc.contributor.author","Suren, Anette"],["dc.contributor.author","Osmers, Rüdiger"],["dc.contributor.author","Köhler, Michael"],["dc.contributor.author","Legler, Tobias Joerg"],["dc.date.accessioned","2021-06-01T10:50:22Z"],["dc.date.available","2021-06-01T10:50:22Z"],["dc.date.issued","2002"],["dc.description.abstract","Real-time PCR methods for the detection of RHD and the C, c, and E allele of RHCE were applied for the prediction of fetal Rh phenotype using maternal plasma. In one of 36 samples investigated the DNA extraction failed. When we tested the remaining 35 samples for Rh antigens which were absent on the mother's red cells, the fetal D-status was correctly determined in 26 of 27 cases (1 false negative). Fetal C was tested correctly in 23 samples, c was true positive in the only c-negative woman and the fetal E-status was correctly determined in 35 cases. In conclusion real-time PCR of maternal plasma is a non-invasive method to determine fetal RH genotype. However, more studies are required for routine applications because the method is not 100% sensitive. (C) 2002 Elsevier Science Ltd. All rights reserved."],["dc.identifier.doi","10.1016/S1473-0502(02)00068-X"],["dc.identifier.isi","000179790300006"],["dc.identifier.pmid","12509216"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86635"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Pergamon-elsevier Science Ltd"],["dc.relation.issn","1473-0502"],["dc.title","Prediction of fetal Rh D and Rh CcEe phenotype from maternal plasma with real-time polymerase chain reaction"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dspace.entity.type","Publication"]]