Stülke, Jörg

[["dc.bibliographiccitation.firstpage","6102"],["dc.bibliographiccitation.issue","21"],["dc.bibliographiccitation.journal","Nucleic Acids Research"],["dc.bibliographiccitation.lastpage","6115"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Schilling, Oliver"],["dc.contributor.author","Herzberg, Christina"],["dc.contributor.author","Hertrich, Tina"],["dc.contributor.author","Voersmann, Hanna"],["dc.contributor.author","Jessen, Dirk"],["dc.contributor.author","Huebner, Sebastian"],["dc.contributor.author","Titgemeyer, Fritz"],["dc.contributor.author","Stuelke, Joerg"],["dc.date.accessioned","2018-11-07T08:55:16Z"],["dc.date.available","2018-11-07T08:55:16Z"],["dc.date.issued","2006"],["dc.description.abstract","Regulatory systems often evolve by duplication of ancestral systems and subsequent specialization of the components of the novel signal transduction systems. In the Gram-positive soil bacterium Bacillus subtilis, four homologous antitermination systems control the expression of genes involved in the metabolism of glucose, sucrose and beta-glucosides. Each of these systems is made up of a sensory sugar permease that does also act as phosphotransferase, an antitermination protein, and a RNA switch that is composed of two mutually exclusive structures, a RNA antiterminator (RAT) and a transcriptional terminator. We have studied the contributions of sugar specificity of the permeases, carbon catabolite repression, and protein-RAT recognition for the straightness of the signalling chains. We found that the beta-glucoside permease BglP does also have a minor activity in glucose transport. However, this activity is irrelevant under physiological conditions since carbon catabolite repression in the presence of glucose prevents the synthesis of the beta-glucoside permease. Reporter gene studies, in vitro RNA-protein interaction analyzes and northern blot transcript analyzes revealed that the interactions between the antiterminator proteins and their RNA targets are the major factor contributing to regulatory specificity. Both structural features in the RATs and individual bases are important specificity determinants. Our study revealed that the specificity of protein-RNA interactions, substrate specificity of the permeases as well as the general mechanism of carbon catabolite repression together allow to keep the signalling chains straight and to avoid excessive cross-talk between the systems."],["dc.identifier.doi","10.1093/nar/gkl733"],["dc.identifier.isi","000242716800010"],["dc.identifier.pmid","17074746"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?goescholar/4130"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22869"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Oxford Univ Press"],["dc.relation.issn","0305-1048"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Keeping signals straight in transcription regulation: specificity determinants for the interaction of a family of conserved bacterial RNA-protein couples"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","804"],["dc.bibliographiccitation.journal","Frontiers in Microbiology"],["dc.bibliographiccitation.volume","7"],["dc.contributor.author","Gundlach, Jan"],["dc.contributor.author","Rath, Hermann"],["dc.contributor.author","Herzberg, Christina"],["dc.contributor.author","Maeder, Ulrike"],["dc.contributor.author","Stuelke, Joerg"],["dc.date.accessioned","2018-11-07T10:14:07Z"],["dc.date.available","2018-11-07T10:14:07Z"],["dc.date.issued","2016"],["dc.description.abstract","The Gram-positive model organism Bacillus subtilis produces the essential second messenger signaling nucleotide cyclic di-AMP. In B. subtilis and other bacteria, c-di-AMP has been implicated in diverse functions such as control of metabolism, cell division and cell wall synthesis, and potassium transport. To enhance our understanding of the multiple functions of this second messenger, we have studied the consequences of c-di-AMP accumulation at a global level by a transcriptome analysis. C-di-AMP accumulation affected the expression of about 700 genes, among them the two major operons required for biofilm formation. The expression of both operons was severely reduced both in the laboratory and a non-domesticated strain upon accumulation of c-di-AMP. In excellent agreement, the corresponding strain was unable to form complex colonies. In B. subtilis, the transcription factor SinR controls the expression of biofilm genes by binding to their promoter regions resulting in transcription repression. Inactivation of the sing gene restored biofilm formation even at high intracellular c-di-AMP concentrations suggesting that the second messenger acts upstream of SinR in the signal transduction pathway. As c-di-AMP accumulation did not affect the intracellular levels of SinR, we conclude that the nucleotide affects the activity of SinR."],["dc.description.sponsorship","Open-Access-Publikationsfonds 2016"],["dc.identifier.doi","10.3389/fmicb.2016.00804"],["dc.identifier.isi","000376484900001"],["dc.identifier.pmid","27252699"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/13293"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40564"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Frontiers Media Sa"],["dc.relation.issn","1664-302X"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Second Messenger Signaling in Bacillus subtilis: Accumulation of Cyclic di-AMP Inhibits Biofilm Formation"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e1009092"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLoS Genetics"],["dc.bibliographiccitation.volume","17"],["dc.contributor.author","Krüger, Larissa"],["dc.contributor.author","Herzberg, Christina"],["dc.contributor.author","Rath, Hermann"],["dc.contributor.author","Pedreira, Tiago"],["dc.contributor.author","Ischebeck, Till"],["dc.contributor.author","Poehlein, Anja"],["dc.contributor.author","Gundlach, Jan"],["dc.contributor.author","Daniel, Rolf"],["dc.contributor.author","Völker, Uwe"],["dc.contributor.author","Mäder, Ulrike"],["dc.contributor.author","Stülke, Jörg"],["dc.date.accessioned","2021-04-14T08:29:55Z"],["dc.date.available","2021-04-14T08:29:55Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1371/journal.pgen.1009092"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/83034"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","1553-7404"],["dc.rights","CC BY 4.0"],["dc.title","Essentiality of c-di-AMP in Bacillus subtilis: Bypassing mutations converge in potassium and glutamate homeostasis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","12"],["dc.contributor.author","Krüger, Larissa"],["dc.contributor.author","Herzberg, Christina"],["dc.contributor.author","Wicke, Dennis"],["dc.contributor.author","Bähre, Heike"],["dc.contributor.author","Heidemann, Jana L."],["dc.contributor.author","Dickmanns, Achim"],["dc.contributor.author","Schmitt, Kerstin"],["dc.contributor.author","Ficner, Ralf"],["dc.contributor.author","Stülke, Jörg"],["dc.date.accessioned","2021-04-14T08:28:38Z"],["dc.date.available","2021-04-14T08:28:38Z"],["dc.date.issued","2021"],["dc.description.sponsorship","Open-Access-Publikationsfonds 2021"],["dc.identifier.doi","10.1038/s41467-021-21306-0"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/82669"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-399"],["dc.relation.eissn","2041-1723"],["dc.relation.orgunit","Institut für Mikrobiologie und Genetik"],["dc.rights","CC BY 4.0"],["dc.title","A meet-up of two second messengers: the c-di-AMP receptor DarB controls (p)ppGpp synthesis in Bacillus subtilis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]