Fernández Busnadiego, Rubén

[["dc.bibliographiccitation.firstpage","296"],["dc.bibliographiccitation.issue","7794"],["dc.bibliographiccitation.journal","Nature"],["dc.bibliographiccitation.lastpage","300"],["dc.bibliographiccitation.volume","578"],["dc.contributor.author","Yasuda, Sayaka"],["dc.contributor.author","Tsuchiya, Hikaru"],["dc.contributor.author","Kaiho, Ai"],["dc.contributor.author","Guo, Qiang"],["dc.contributor.author","Ikeuchi, Ken"],["dc.contributor.author","Endo, Akinori"],["dc.contributor.author","Arai, Naoko"],["dc.contributor.author","Ohtake, Fumiaki"],["dc.contributor.author","Murata, Shigeo"],["dc.contributor.author","Inada, Toshifumi"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Tanaka, Keiji"],["dc.contributor.author","Saeki, Yasushi"],["dc.date.accessioned","2020-12-10T18:10:02Z"],["dc.date.available","2020-12-10T18:10:02Z"],["dc.date.issued","2020"],["dc.identifier.doi","10.1038/s41586-020-1982-9"],["dc.identifier.pmid","32025036"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73832"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/30"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","Stress- and ubiquitylation-dependent phase separation of the proteasome"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Saha, Itika"],["dc.contributor.author","Yuste-Checa, Patricia"],["dc.contributor.author","Silva Padilha, Miguel Da"],["dc.contributor.author","Guo, Qiang"],["dc.contributor.author","Körner, Roman"],["dc.contributor.author","Holthusen, Hauke"],["dc.contributor.author","Trinkaus, Victoria A."],["dc.contributor.author","Dudanova, Irina"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Sanders, David W."],["dc.contributor.author","Gautam, Saurabh"],["dc.contributor.author","Diamond, Marc I."],["dc.contributor.author","Ulrich Hartl, F."],["dc.contributor.author","Hipp, Mark S."],["dc.date.accessioned","2022-04-06T12:10:37Z"],["dc.date.available","2022-04-06T12:10:37Z"],["dc.date.issued","2022"],["dc.identifier.doi","10.1101/2022.02.18.481043"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/106453"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/449"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","The AAA+ chaperone VCP disaggregates Tau fibrils and generates aggregate seeds"],["dc.type","preprint"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Huang, Bin"],["dc.contributor.author","Guo, Qiang"],["dc.contributor.author","Niedermeier, Marie L."],["dc.contributor.author","Cheng, Jingdong"],["dc.contributor.author","Engler, Tatjana"],["dc.contributor.author","Maurer, Melanie"],["dc.contributor.author","Pautsch, Alexander"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Stengel, Florian"],["dc.contributor.author","Kochanek, Stefan"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.date.accessioned","2022-02-23T16:35:26Z"],["dc.date.available","2022-02-23T16:35:26Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1101/2021.02.02.429316"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100375"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/231"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","PolyQ expansion does not alter the Huntingtin-HAP40 complex"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Riemenschneider, Henrick"],["dc.contributor.author","Guo, Qiang"],["dc.contributor.author","Bader, Jakob"],["dc.contributor.author","Frottin, Frédéric"],["dc.contributor.author","Farny, Daniel"],["dc.contributor.author","Kleinberger, Gernot"],["dc.contributor.author","Haass, Christian"],["dc.contributor.author","Mann, Matthias"],["dc.contributor.author","Hartl, F. Ulrich"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Hipp, Mark S."],["dc.contributor.author","Meissner, Felix"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Edbauer, Dieter"],["dc.date.accessioned","2022-02-23T16:35:41Z"],["dc.date.available","2022-02-23T16:35:41Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1101/2021.03.15.435268"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100379"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/288"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","Gel-like inclusions of C-terminal fragments of TDP-43 sequester and inhibit proteasomes in neurons"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","725"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","740"],["dc.bibliographiccitation.volume","201"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Asano, Shoh"],["dc.contributor.author","Oprisoreanu, Ana-Maria"],["dc.contributor.author","Sakata, Eri"],["dc.contributor.author","Doengi, Michael"],["dc.contributor.author","Kochovski, Zdravko"],["dc.contributor.author","Zürner, Magdalena"],["dc.contributor.author","Stein, Valentin"],["dc.contributor.author","Schoch, Susanne"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Lučić, Vladan"],["dc.date.accessioned","2022-03-01T11:46:33Z"],["dc.date.available","2022-03-01T11:46:33Z"],["dc.date.issued","2013"],["dc.description.abstract","Synaptic vesicles are embedded in a complex filamentous network at the presynaptic terminal. Before fusion, vesicles are linked to the active zone (AZ) by short filaments (tethers). The identity of the molecules that form and regulate tethers remains unknown, but Rab3-interacting molecule (RIM) is a prominent candidate, given its central role in AZ organization. In this paper, we analyzed presynaptic architecture of RIM1α knockout (KO) mice by cryo–electron tomography. In stark contrast to previous work on dehydrated, chemically fixed samples, our data show significant alterations in vesicle distribution and AZ tethering that could provide a structural basis for the functional deficits of RIM1α KO synapses. Proteasome inhibition reversed these structural defects, suggesting a functional recovery confirmed by electrophysiological recordings. Altogether, our results not only point to the ubiquitin–proteasome system as an important regulator of presynaptic architecture and function but also show that the tethering machinery plays a critical role in exocytosis, converging into a structural model of synaptic vesicle priming by RIM1α."],["dc.description.abstract","Synaptic vesicles are embedded in a complex filamentous network at the presynaptic terminal. Before fusion, vesicles are linked to the active zone (AZ) by short filaments (tethers). The identity of the molecules that form and regulate tethers remains unknown, but Rab3-interacting molecule (RIM) is a prominent candidate, given its central role in AZ organization. In this paper, we analyzed presynaptic architecture of RIM1α knockout (KO) mice by cryo–electron tomography. In stark contrast to previous work on dehydrated, chemically fixed samples, our data show significant alterations in vesicle distribution and AZ tethering that could provide a structural basis for the functional deficits of RIM1α KO synapses. Proteasome inhibition reversed these structural defects, suggesting a functional recovery confirmed by electrophysiological recordings. Altogether, our results not only point to the ubiquitin–proteasome system as an important regulator of presynaptic architecture and function but also show that the tethering machinery plays a critical role in exocytosis, converging into a structural model of synaptic vesicle priming by RIM1α."],["dc.identifier.doi","10.1083/jcb.201206063"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103710"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1540-8140"],["dc.relation.issn","0021-9525"],["dc.title","Cryo–electron tomography reveals a critical role of RIM1α in synaptic vesicle tethering"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","179"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Cell"],["dc.bibliographiccitation.lastpage","187.e10"],["dc.bibliographiccitation.volume","171"],["dc.contributor.author","Bäuerlein, Felix J.B."],["dc.contributor.author","Saha, Itika"],["dc.contributor.author","Mishra, Archana"],["dc.contributor.author","Kalemanov, Maria"],["dc.contributor.author","Martínez-Sánchez, Antonio"],["dc.contributor.author","Klein, Rüdiger"],["dc.contributor.author","Dudanova, Irina"],["dc.contributor.author","Hipp, Mark S."],["dc.contributor.author","Hartl, F. Ulrich"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.date.accessioned","2022-03-01T11:45:03Z"],["dc.date.available","2022-03-01T11:45:03Z"],["dc.date.issued","2017"],["dc.identifier.doi","10.1016/j.cell.2017.08.009"],["dc.identifier.pii","S0092867417309340"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103197"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","0092-8674"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","In Situ Architecture and Cellular Interactions of PolyQ Inclusions"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","145"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Cell Biology"],["dc.bibliographiccitation.lastpage","156"],["dc.bibliographiccitation.volume","188"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Zuber, Benoît"],["dc.contributor.author","Maurer, Ulrike Elisabeth"],["dc.contributor.author","Cyrklaff, Marek"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Lučić, Vladan"],["dc.date.accessioned","2022-03-01T11:46:33Z"],["dc.date.available","2022-03-01T11:46:33Z"],["dc.date.issued","2010"],["dc.description.abstract","The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles did not make membrane to membrane contact with the active zone but were instead linked to it by tethers of different length. Our observations are consistent with an exocytosis model in which vesicles are first anchored by long (>5 nm) tethers that give way to multiple short tethers once vesicles enter the readily releasable pool. The formation of short tethers was inhibited by tetanus toxin, indicating that it depends on soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor complex assembly. Vesicles were extensively interlinked via a set of connectors that underwent profound rearrangements upon synaptic stimulation and okadaic acid treatment, suggesting a role of these connectors in synaptic vesicle mobilization and neurotransmitter release."],["dc.description.abstract","The presynaptic terminal contains a complex network of filaments whose precise organization and functions are not yet understood. The cryoelectron tomography experiments reported in this study indicate that these structures play a prominent role in synaptic vesicle release. Docked synaptic vesicles did not make membrane to membrane contact with the active zone but were instead linked to it by tethers of different length. Our observations are consistent with an exocytosis model in which vesicles are first anchored by long (>5 nm) tethers that give way to multiple short tethers once vesicles enter the readily releasable pool. The formation of short tethers was inhibited by tetanus toxin, indicating that it depends on soluble N-ethyl-maleimide sensitive fusion protein attachment protein receptor complex assembly. Vesicles were extensively interlinked via a set of connectors that underwent profound rearrangements upon synaptic stimulation and okadaic acid treatment, suggesting a role of these connectors in synaptic vesicle mobilization and neurotransmitter release."],["dc.identifier.doi","10.1083/jcb.200908082"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103709"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.eissn","1540-8140"],["dc.relation.issn","0021-9525"],["dc.title","Quantitative analysis of the native presynaptic cytomatrix by cryoelectron tomography"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","503"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Structural Biology"],["dc.bibliographiccitation.lastpage","514"],["dc.bibliographiccitation.volume","196"],["dc.contributor.author","Lučić, Vladan"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Laugks, Ulrike"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.date.accessioned","2022-03-01T11:45:15Z"],["dc.date.available","2022-03-01T11:45:15Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.1016/j.jsb.2016.10.004"],["dc.identifier.pii","S104784771630209X"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/103267"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-531"],["dc.relation.issn","1047-8477"],["dc.rights.uri","https://www.elsevier.com/tdm/userlicense/1.0/"],["dc.title","Hierarchical detection and analysis of macromolecular complexes in cryo-electron tomograms using Pyto software"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.contributor.author","Riera-Tur, Irene"],["dc.contributor.author","Schäfer, Tillman"],["dc.contributor.author","Hornburg, Daniel"],["dc.contributor.author","Mishra, Archana"],["dc.contributor.author","Fernández-Mosquera, Lorena"],["dc.contributor.author","Feigenbutz, Dennis"],["dc.contributor.author","Auer, Patrick"],["dc.contributor.author","Mann, Matthias"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Klein, Rüdiger"],["dc.contributor.author","Meissner, Felix"],["dc.contributor.author","Raimundo, Nuno"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.contributor.author","Dudanova, Irina"],["dc.date.accessioned","2022-02-23T10:02:21Z"],["dc.date.available","2022-02-23T10:02:21Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1101/2019.12.16.877431"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/100230"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/5"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","Amyloid-like aggregates cause lysosomal defects in neurons via gain-of-function toxicity"],["dc.type","preprint"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.journal","Structure"],["dc.contributor.author","Huang, Bin"],["dc.contributor.author","Guo, Qiang"],["dc.contributor.author","Niedermeier, Marie L."],["dc.contributor.author","Cheng, Jingdong"],["dc.contributor.author","Engler, Tatjana"],["dc.contributor.author","Maurer, Melanie"],["dc.contributor.author","Pautsch, Alexander"],["dc.contributor.author","Baumeister, Wolfgang"],["dc.contributor.author","Stengel, Florian"],["dc.contributor.author","Fernández Busnadiego, Rubén"],["dc.date.accessioned","2021-06-01T10:49:56Z"],["dc.date.available","2021-06-01T10:49:56Z"],["dc.date.issued","2021"],["dc.identifier.doi","10.1016/j.str.2021.04.003"],["dc.identifier.pmid","33909994"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/86467"],["dc.identifier.url","https://mbexc.uni-goettingen.de/literature/publications/274"],["dc.language.iso","en"],["dc.notes.intern","DOI-Import GROB-425"],["dc.relation","EXC 2067: Multiscale Bioimaging"],["dc.relation.issn","0969-2126"],["dc.relation.workinggroup","RG Fernández-Busnadiego (Structural Cell Biology)"],["dc.title","Pathological polyQ expansion does not alter the conformation of the Huntingtin-HAP40 complex"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.subtype","original_ja"],["dspace.entity.type","Publication"]]