Wahl, Markus C.

[["dc.bibliographiccitation.artnumber","e06935"],["dc.bibliographiccitation.journal","eLife"],["dc.bibliographiccitation.volume","4"],["dc.contributor.author","Siebert, Matthias"],["dc.contributor.author","Böhme, Mathias"],["dc.contributor.author","Driller, Jan H."],["dc.contributor.author","Babikir, Husam"],["dc.contributor.author","Mampell, Malou M."],["dc.contributor.author","Rey, Ulises"],["dc.contributor.author","Ramesh, Niraja"],["dc.contributor.author","Matkovic, Tanja"],["dc.contributor.author","Holton, Nicole"],["dc.contributor.author","Reddy-Alla, Suneel"],["dc.contributor.author","Göttfert, Fabian"],["dc.contributor.author","Kamin, Dirk"],["dc.contributor.author","Quentin, Christine"],["dc.contributor.author","Klinedinst, Susan"],["dc.contributor.author","Andlauer, Till F. M."],["dc.contributor.author","Hell, Stefan"],["dc.contributor.author","Collins, Catherine A."],["dc.contributor.author","Wahl, Markus C."],["dc.contributor.author","Loll, Bernhard"],["dc.contributor.author","Sigrist, Stephan J."],["dc.date.accessioned","2017-09-07T11:43:37Z"],["dc.date.available","2017-09-07T11:43:37Z"],["dc.date.issued","2015"],["dc.description.abstract","Synaptic vesicles (SVs) fuse at active zones (AZs) covered by a protein scaffold, at Drosophila synapses comprised of ELKS family member Bruchpilot (BRP) and RIM-binding protein (RBP). We here demonstrate axonal co-transport of BRP and RBP using intravital live imaging, with both proteins co-accumulating in axonal aggregates of several transport mutants. RBP, via its C-terminal Src-homology 3 (SH3) domains, binds Aplip1/JIP1, a transport adaptor involved in kinesin-dependent SV transport. We show in atomic detail that RBP C-terminal SH3 domains bind a proline-rich (PxxP) motif of Aplip1/JIP1 with submicromolar affinity. Pointmutating this PxxP motif provoked formation of ectopic AZ-like structures at axonal membranes. Direct interactions between AZ proteins and transport adaptors seem to provide complex avidity and shield synaptic interaction surfaces of pre-assembled scaffold protein transport complexes, thus, favouring physiological synaptic AZ assembly over premature assembly at axonal membranes."],["dc.identifier.doi","10.7554/eLife.06935"],["dc.identifier.gro","3141845"],["dc.identifier.isi","000359681600001"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/1712"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","2050-084X"],["dc.title","A high affinity RIM-binding protein/Aplip1 interaction prevents the formation of ectopic axonal active zones"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]