Zeug, André

[["dc.bibliographiccitation.artnumber","014022"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Journal of Biomedical Optics"],["dc.bibliographiccitation.volume","14"],["dc.contributor.author","Weigel, Arwed"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Zeug, Andre"],["dc.date.accessioned","2018-11-07T08:34:48Z"],["dc.date.available","2018-11-07T08:34:48Z"],["dc.date.issued","2009"],["dc.description.abstract","The essential feature of the confocal laser scanning microscope (cLSM) is the generation of optical sections by the removal of out-of-focus light. About ten years ago, structured illumination microscopy (SIM) was introduced as an alternative method for obtaining optical sections from biological specimens. Here we compare the resolution of the ApoTome (commercial SIM by Zeiss) to that achieved by a cLSM (Zeiss LSM 510). If fluorescent beads are used as test objects, then the ApoTome will achieve a lower axial resolution than the cLSM. In contrast to that, its lateral resolution scores slightly better. If subresolution homogeneous fluorescent layers are used as test objects, then the ApoTome will achieve a higher axial resolution than the cLSM. The ApoTome's axial resolution is homogeneous over the field-of-view while that of the cLSM changes markedly. Finally, the anisotropy of the ApoTome's resolution was found to be negligible for standard applications while its capability to resolve fine structures within stained tissue slices is limited to one or two cell layers and thus worse than in the cLSM. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3083439]"],["dc.description.sponsorship","Deutsche Forschungsgemeinschaft"],["dc.identifier.doi","10.1117/1.3083439"],["dc.identifier.isi","000264551900027"],["dc.identifier.pmid","19256710"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7767"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17904"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Spie-soc Photoptical Instrumentation Engineers"],["dc.relation.issn","1083-3668"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Resolution in the ApoTome and the confocal laser scanning microscope: comparison"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Nature Communications"],["dc.bibliographiccitation.volume","10"],["dc.contributor.author","Gorinski, Nataliya"],["dc.contributor.author","Bijata, Monika"],["dc.contributor.author","Prasad, Sonal"],["dc.contributor.author","Wirth, Alexander"],["dc.contributor.author","Abdel Galil, Dalia"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Bazovkina, Daria"],["dc.contributor.author","Kondaurova, Elena"],["dc.contributor.author","Kulikova, Elizabeth"],["dc.contributor.author","Ilchibaeva, Tatiana"],["dc.contributor.author","Zareba-Koziol, Monika"],["dc.contributor.author","Papaleo, Francesco"],["dc.contributor.author","Scheggia, Diego"],["dc.contributor.author","Kochlamazashvili, Gaga"],["dc.contributor.author","Dityatev, Alexander"],["dc.contributor.author","Smyth, Ian"],["dc.contributor.author","Krzystyniak, Adam"],["dc.contributor.author","Wlodarczyk, Jakub"],["dc.contributor.author","Richter, Diethelm W."],["dc.contributor.author","Strekalova, Tatyana"],["dc.contributor.author","Sigrist, Stephan"],["dc.contributor.author","Bang, Claudia"],["dc.contributor.author","Hobuß, Lisa"],["dc.contributor.author","Fiedler, Jan"],["dc.contributor.author","Thum, Thomas"],["dc.contributor.author","Naumenko, Vladimir S."],["dc.contributor.author","Pandey, Ghanshyam"],["dc.contributor.author","Ponimaskin, Evgeni"],["dc.date.accessioned","2020-12-10T18:09:50Z"],["dc.date.available","2020-12-10T18:09:50Z"],["dc.date.issued","2019"],["dc.identifier.doi","10.1038/s41467-019-11876-5"],["dc.identifier.eissn","2041-1723"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/16768"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/73772"],["dc.language.iso","en"],["dc.notes.intern","DOI Import GROB-354"],["dc.notes.intern","Merged from goescholar"],["dc.rights","CC BY 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by/4.0"],["dc.title","Attenuated palmitoylation of serotonin receptor 5-HT1A affects receptor function and contributes to depression-like behaviors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","3791"],["dc.bibliographiccitation.issue","9"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","3800"],["dc.bibliographiccitation.volume","96"],["dc.contributor.author","Neher, Richard A."],["dc.contributor.author","Mitkovski, Miso"],["dc.contributor.author","Kirchhoff, Frank"],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Theis, Fabian J."],["dc.contributor.author","Zeug, Andre"],["dc.date.accessioned","2018-11-07T08:30:00Z"],["dc.date.available","2018-11-07T08:30:00Z"],["dc.date.issued","2009"],["dc.description.abstract","Methods of blind source separation are used in many contexts to separate composite data sets according to their sources. Multiply labeled fluorescence microscopy images represent such sets, in which the sources are the individual labels. Then distributions are the quantities of interest and have to be extracted from the images. This is often challenging, since the recorded emission spectra of fluorescent dyes are environment- and instrument-specific. We have developed a nonnegative matrix factorization (NMF) algorithm to detect and separate spectrally distinct components of multiply labeled fluorescence images. It operates on spectrally resolved images and delivers both the emission spectra of the identified components and images of their abundance. We tested the proposed method using biological samples labeled with up to four spectrally overlapping fluorescent labels. In most cases, NMF accurately decomposed the images into contributions of individual dyes. However, the Solutions are not unique when spectra overlap strongly or when images are diffuse in their structure. To arrive at satisfactory results in such cases, we extended NMF to incorporate preexisting qualitative knowledge about spectra and label distributions. We show how data acquired through excitations at two or three different wavelengths can be integrated and that multiple excitations greatly facilitate the decomposition. By allowing reliable decomposition in cases where the spectra of the individual labels are not known or are known only inaccurately, the proposed algorithms greatly extend the range of questions that can be addressed with quantitative microscopy."],["dc.identifier.doi","10.1016/j.bpj.2008.10.068"],["dc.identifier.isi","000266397700033"],["dc.identifier.pmid","19413985"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7763"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/16787"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Blind Source Separation Techniques for the Decomposition of Multiply Labeled Fluorescence Images"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1752"],["dc.bibliographiccitation.issue","12"],["dc.bibliographiccitation.journal","Molecular Psychiatry"],["dc.bibliographiccitation.lastpage","1767"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Hassouna, I."],["dc.contributor.author","Ott, C."],["dc.contributor.author","Wüstefeld, L."],["dc.contributor.author","Offen, N."],["dc.contributor.author","Neher, R. A."],["dc.contributor.author","Mitkovski, M."],["dc.contributor.author","Winkler, D."],["dc.contributor.author","Sperling, S."],["dc.contributor.author","Fries, L."],["dc.contributor.author","Goebbels, S."],["dc.contributor.author","Vreja, I. C."],["dc.contributor.author","Hagemeyer, N."],["dc.contributor.author","Dittrich, M."],["dc.contributor.author","Rossetti, M. F."],["dc.contributor.author","Kröhnert, K."],["dc.contributor.author","Hannke, K."],["dc.contributor.author","Boretius, S."],["dc.contributor.author","Zeug, A."],["dc.contributor.author","Höschen, C."],["dc.contributor.author","Dandekar, T."],["dc.contributor.author","Dere, E."],["dc.contributor.author","Neher, E."],["dc.contributor.author","Rizzoli, S. O."],["dc.contributor.author","Nave, K.-A."],["dc.contributor.author","Sirén, A.-L."],["dc.contributor.author","Ehrenreich, H."],["dc.date.accessioned","2017-09-07T11:53:29Z"],["dc.date.available","2017-09-07T11:53:29Z"],["dc.date.issued","2016"],["dc.description.abstract","Recombinant human erythropoietin (EPO) improves cognitive performance in neuropsychiatric diseases ranging from schizophrenia and multiple sclerosis to major depression and bipolar disease. This consistent EPO effect on cognition is independent of its role in hematopoiesis. The cellular mechanisms of action in brain, however, have remained unclear. Here we studied healthy young mice and observed that 3-week EPO administration was associated with an increased number of pyramidal neurons and oligodendrocytes in the hippocampus of ~20%. Under constant cognitive challenge, neuron numbers remained elevated until >6 months of age. Surprisingly, this increase occurred in absence of altered cell proliferation or apoptosis. After feeding a 15N-leucine diet, we used nanoscopic secondary ion mass spectrometry, and found that in EPO-treated mice, an equivalent number of neurons was defined by elevated 15N-leucine incorporation. In EPO-treated NG2-Cre-ERT2 mice, we confirmed enhanced differentiation of preexisting oligodendrocyte precursors in the absence of elevated DNA synthesis. A corresponding analysis of the neuronal lineage awaits the identification of suitable neuronal markers. In cultured neurospheres, EPO reduced Sox9 and stimulated miR124, associated with advanced neuronal differentiation. We are discussing a resulting working model in which EPO drives the differentiation of non-dividing precursors in both (NG2+) oligodendroglial and neuronal lineages. As endogenous EPO expression is induced by brain injury, such a mechanism of adult neurogenesis may be relevant for central nervous system regeneration."],["dc.identifier.doi","10.1038/mp.2015.212"],["dc.identifier.fs","620695"],["dc.identifier.gro","3145088"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/14225"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/2785"],["dc.language.iso","en"],["dc.notes.intern","Crossref Import"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.issn","1359-4184"],["dc.rights","CC BY-NC-ND 4.0"],["dc.rights.uri","https://creativecommons.org/licenses/by-nc-nd/4.0"],["dc.title","Revisiting adult neurogenesis and the role of erythropoietin for neuronal and oligodendroglial differentiation in the hippocampus"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.peerReviewed","no"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5412"],["dc.bibliographiccitation.issue","11"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","5423"],["dc.bibliographiccitation.volume","95"],["dc.contributor.author","Salonikidis, Petrus S."],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Kobe, Fritz"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.contributor.author","Richter, Diethelm W."],["dc.date.accessioned","2018-11-07T11:08:21Z"],["dc.date.available","2018-11-07T11:08:21Z"],["dc.date.issued","2008"],["dc.description.abstract","Forster resonance energy transfer (FRET)-based biosensors for the quantitative analysis of intracellular signaling, including sensors for monitoring cyclic adenosine monophosphate (cAMP), are of increasing interest. The measurement of the donor/acceptor emission ratio in tandem biosensors excited at the donor excitation wavelength is a commonly used technique. A general problem, however, is that this ratio varies not only with the changes in cAMP concentration but also with the changes of the ionic environment or other factors affecting the folding probability of the fluorophores. Here, we use a spectral FRET analysis on the basis of two excitation wavelengths to obtain a reliable measure of the absolute cAMP concentrations with high temporal and spatial resolution by using an \"exchange protein directly activated by cAMP\". In this approach, FRET analysis is simplified and does not require additional calibration routines. The change in FRET efficiency (E) of the biosensor caused by [cAMP] changes was determined as Delta E=15%, whereas E varies between 35% at low and 20% at high [cAMP], allowing quantitative measurement of cAMP concentration in the range from 150 nM to 15 mu M. The method described is also suitable for other FRET-based biosensors with a 1: 1 donor/acceptor stoichiometry. As a proof of principle, we measured the specially resolved cAMP concentration within living cells and determined the dynamic changes of cAMP levels after stimulation of the Gs-coupled serotonin receptor subtype 7 (5-HT7)."],["dc.identifier.doi","10.1529/biophysj.107.125666"],["dc.identifier.isi","000260999500039"],["dc.identifier.pmid","18708470"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7766"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/52756"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Biophysical Soc"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Quantitative Measurement of cAMP Concentration Using an Exchange Protein Directly Activated by a cAMP-Based FRET-Sensor"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","1503"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","Biochimica et Biophysica Acta (BBA) - Molecular Cell Research"],["dc.bibliographiccitation.lastpage","1516"],["dc.bibliographiccitation.volume","1783"],["dc.contributor.author","Kobe, Fritz"],["dc.contributor.author","Renner, Ute"],["dc.contributor.author","Woehler, Andrew"],["dc.contributor.author","Wlodarczyk, Jakub"],["dc.contributor.author","Papusheva, Ekaterina"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Richter, Diethelm W."],["dc.contributor.author","Neher, Erwin"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.date.accessioned","2018-11-07T11:12:17Z"],["dc.date.available","2018-11-07T11:12:17Z"],["dc.date.issued","2008"],["dc.description.abstract","In the present study we analyzed the oligomerization state of the serotonin 5-HT1A receptor and studied oligomerization dynamics in living cells. We also investigated the role of receptor palmitoylation in this process. Biochemical analysis performed in neuroblastoma N1E-115 cells demonstrated that both palmitoylated and non-palmitoylated 5-HT1A receptors form homo-oligomers and that the prevalent receptor species at the plasma membrane are dimers. A combination of an acceptor-photobleaching FRET approach with fluorescence lifetime measurements verified the interaction of CFP- and YFP-labeled wild-type as well as acylation-deficient 5-HT1A receptors at the plasma membrane of living cells. Using a novel FRET technique based on the spectral analysis we also confirmed the specific nature of receptor oligomerization. The analysis of oligomerization dynamics revealed that apparent FRET efficiency measured for wild-type oligomers significantly decreased in response to agonist stimulation, and our combined results suggest that this decrease was mediated by accumulation of FRET-negative complexes rather than by dissociation of oligomers to monomers. In contrast, the agonist-mediated decrease of FRET signal was completely abolished in oligomers composed by non-palmitoylated receptor mutants, demonstrating the importance of palmitoylation in modulation of the structure of oligomers. (C) 2008 Elsevier B.V. All rights reserved."],["dc.identifier.doi","10.1016/j.bbamcr.2008.02.021"],["dc.identifier.isi","000257641600004"],["dc.identifier.pmid","18381076"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7758"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/53629"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Elsevier Science Bv"],["dc.relation.issn","0167-4889"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Stimulation- and palmitoylation-dependent changes in oligomeric conformation of serotonin 5-HT1A receptors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","23419"],["dc.bibliographiccitation.issue","26"],["dc.bibliographiccitation.journal","Journal of Biological Chemistry"],["dc.bibliographiccitation.lastpage","23431"],["dc.bibliographiccitation.volume","286"],["dc.contributor.author","Salonikidis, Petrus S."],["dc.contributor.author","Niebert, Marcus"],["dc.contributor.author","Ullrich, Tim"],["dc.contributor.author","Bao, Guobin"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Richter, Diethelm W."],["dc.date.accessioned","2018-11-07T08:54:48Z"],["dc.date.available","2018-11-07T08:54:48Z"],["dc.date.issued","2011"],["dc.description.abstract","Ratiometric measurements with FRET-based biosensors in living cells using a single fluorescence excitation wavelength are often affected by a significant ion sensitivity and the aggregation behavior of the FRET pair. This is an important problem for quantitative approaches. Here we report on the influence of physiological ion concentration changes on quantitative ratiometric measurements by comparing different FRET pairs for a cAMP-detecting biosensor. We exchanged the enhanced CFP/enhanced YFP FRET pair of an established Epac1-based biosensor by the fluorophores mCerulean/mCitrine. In the case of enhanced CFP/enhanced YFP, we showed that changes in proton, and (to a lesser extent) chloride ion concentrations result in incorrect ratiometric FRET signals, which may exceed the dynamic range of the biosensor. Calcium ions have no direct, but an indirect pH-driven effect by mobilizing protons. These ion dependences were greatly eliminated when mCerulean/mCitrine fluorophores were used. For such advanced FRET pairs the biosensor is less sensitive to changes in ion concentration and allows consistent cAMP concentration measurements under different physiological conditions, as occur in metabolically active cells. In addition, we verified that the described FRET pair exchange increased the dynamic range of the FRET efficiency response. The time window for stable experimental conditions was also prolonged by a faster biosensor expression rate in transfected cells and a greatly reduced tendency to aggregate, which reduces cytotoxicity. These properties were verified in functional tests in single cells co-expressing the biosensor and the 5-HT1A receptor."],["dc.identifier.doi","10.1074/jbc.M111.236869"],["dc.identifier.isi","000292025000073"],["dc.identifier.pmid","21454618"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7623"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/22755"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Biochemistry Molecular Biology Inc"],["dc.relation.issn","0021-9258"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","An Ion-insensitive cAMP Biosensor for Long Term Quantitative Ratiometric Fluorescence Resonance Energy Transfer (FRET) Measurements under Variable Physiological Conditions"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","557"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","Infection and Immunity"],["dc.bibliographiccitation.lastpage","564"],["dc.bibliographiccitation.volume","77"],["dc.contributor.author","Ribes, Sandra"],["dc.contributor.author","Ebert, Sandra"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Regen, Tommy"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Bukowski, Stephanie"],["dc.contributor.author","Mildner, Alexander"],["dc.contributor.author","Eiffert, Helmut"],["dc.contributor.author","Hanisch, Uwe-Karsten"],["dc.contributor.author","Hammerschmidt, Sven"],["dc.contributor.author","Nau, Roland"],["dc.date.accessioned","2018-11-07T08:34:37Z"],["dc.date.available","2018-11-07T08:34:37Z"],["dc.date.issued","2009"],["dc.description.abstract","Meningitis and meningoencephalitis caused by Escherichia coli are associated with high rates of mortality. When an infection occurs, Toll-like receptors (TLRs) expressed by microglial cells can recognize pathogen-associated molecular patterns and activate multiple steps in the inflammatory response that coordinate the brain's local defense, such as phagocytosis of invading pathogens. An upregulation of the phagocytic ability of reactive microglia could improve the host defense in immunocompromised patients against pathogens such as E. coli. Here, murine microglial cultures were stimulated with the TLR agonists Pam(3)CSK(4) (TLR1/TLR2), lipopolysaccharide (TLR4), and CpG oligodeoxynucleotide (TLR9) for 24 h. Upon stimulation, levels of tumor necrosis factor alpha and the neutrophil chemoattractant CXCL1 were increased, indicating microglial activation. Phagocytic activity was studied after adding either E. coli DH5 alpha or E. coli K1 strains. After 60 and 90 min of bacterial exposure, the number of ingested bacteria was significantly higher in cells prestimulated with TLR agonists than in unstimulated controls (P < 0.01). Addition of cytochalasin D, an inhibitor of actin polymerization, blocked >90% of phagocytosis. We also analyzed the ability of microglia to kill the ingested E. coli strains. Intracellularly surviving bacteria were quantified at different time points (90, 150, 240, and 360 min) after 90 min of phagocytosis. The number of bacteria killed intracellularly after 6 h was higher in cells primed with the different TLR agonists than in unstimulated microglia. Our data suggest that microglial stimulation by the TLR system can increase bacterial phagocytosis and killing. This approach could improve central nervous system resistance to infections in immunocompromised patients."],["dc.identifier.doi","10.1128/IAI.00903-08"],["dc.identifier.isi","000262776100058"],["dc.identifier.pmid","18981243"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7765"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/17860"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Amer Soc Microbiology"],["dc.relation.issn","0019-9567"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Toll-Like Receptor Prestimulation Increases Phagocytosis of Escherichia coli DH5 alpha and Escherichia coli K1 Strains by Murine Microglial Cells"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","986"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Biophysical Journal"],["dc.bibliographiccitation.lastpage","1000"],["dc.bibliographiccitation.volume","94"],["dc.contributor.author","Wlodarczyk, Jakub"],["dc.contributor.author","Woehler, Andrew"],["dc.contributor.author","Kobe, Fritz"],["dc.contributor.author","Ponimaskin, Evgeni G."],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Neher, Erwin"],["dc.date.accessioned","2018-11-07T11:18:28Z"],["dc.date.available","2018-11-07T11:18:28Z"],["dc.date.issued","2008"],["dc.description.abstract","A method for spectral analysis of Forster resonance energy transfer (FRET) signals is presented, taking into consideration both the contributions of unpaired donor and acceptor fluorophores and the in fluence of incomplete labeling of the interacting partners. It is shown that spectral analysis of intermolecular FRET cannot yield accurate values of the Forster energy transfer efficiency E, unless one of the interactors is in large excess and perfectly labeled. Instead, analysis of donor quenching yields a product of the form Ef(d)p(a), where f(d) is the fraction of donor-type molecules participating in donor-acceptor complexes and p(a) is the labeling probability of the acceptor. Similarly, analysis of sensitized emission yields a product involving Ef(a). The analysis of intramolecular FRET (e. g., of tandem constructs) yields the product Ep(a). We use our method to determine these values for a tandem construct of cyan fluorescent protein and yellow fluorescent protein and compare them with those obtained by standard acceptor photobleaching and fluorescence lifetime measurements. We call the method lux-FRET, since it relies on linear unmixing of spectral components."],["dc.identifier.doi","10.1529/biophysj.107.111773"],["dc.identifier.isi","000252243200025"],["dc.identifier.pmid","17921223"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7747"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/55040"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Cell Press"],["dc.relation.issn","0006-3495"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Analysis of FRET signals in the presence of free donors and acceptors"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","693"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Pflügers Archiv : European Journal of Physiology"],["dc.bibliographiccitation.lastpage","708"],["dc.bibliographiccitation.volume","462"],["dc.contributor.author","Keil, Vera C."],["dc.contributor.author","Funke, Frank"],["dc.contributor.author","Zeug, Andre"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Müller, Michael"],["dc.date.accessioned","2018-09-28T10:25:31Z"],["dc.date.available","2018-09-28T10:25:31Z"],["dc.date.issued","2011"],["dc.description.abstract","Using the mitochondrial potential (ΔΨ(m)) marker JC-1 (5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide) and high-resolution imaging, we functionally analyzed mitochondria in cultured rat hippocampal astrocytes. Ratiometric detection of JC-1 fluorescence identified mitochondria with high and low ΔΨ(m). Mitochondrial density was highest in the perinuclear region, whereas ΔΨ(m) tended to be higher in peripheral mitochondria. Spontaneous ΔΨ(m) fluctuations, representing episodes of increased energization, appeared in individual mitochondria or synchronized in mitochondrial clusters. They continued upon withdrawal of extracellular Ca(2+), but were antagonized by dantrolene or 2-aminoethoxydiphenylborate (2-APB). Fluo-3 imaging revealed local cytosolic Ca(2+) transients with similar kinetics that also were depressed by dantrolene and 2-APB. Massive cellular Ca(2+) load or metabolic impairment abolished ΔΨ(m) fluctuations, occasionally evoking heterogeneous mitochondrial depolarizations. The detected diversity and ΔΨ(m) heterogeneity of mitochondria confirms that even in less structurally polarized cells, such as astrocytes, specialized mitochondrial subpopulations coexist. We conclude that ΔΨ(m) fluctuations are an indication of mitochondrial viability and are triggered by local Ca(2+) release from the endoplasmic reticulum. This spatially confined organelle crosstalk contributes to the functional heterogeneity of mitochondria and may serve to adapt the metabolism of glial cells to the activity and metabolic demand of complex neuronal networks. The established ratiometric JC-1 imaging-especially combined with two-photon microscopy-enables quantitative functional analyses of individual mitochondria as well as the comparison of mitochondrial heterogeneity in different preparations and/or treatment conditions."],["dc.identifier.doi","10.1007/s00424-011-1012-8"],["dc.identifier.pmid","21881871"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7130"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/15857"],["dc.language.iso","en"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","final"],["dc.relation.eissn","1432-2013"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Ratiometric high-resolution imaging of JC-1 fluorescence reveals the subcellular heterogeneity of astrocytic mitochondria"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]