Gwosch, Klaus C.

[["dc.bibliographiccitation.firstpage","962"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","967"],["dc.bibliographiccitation.volume","115"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Gwosch, Klaus C."],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2018-01-17T13:26:27Z"],["dc.date.available","2018-01-17T13:26:27Z"],["dc.date.issued","2018"],["dc.description.abstract","Bioluminescence imaging of single cells is often complicated by the requirement of exogenous luciferins that can be poorly cell-permeable or produce high background signal. Bacterial bioluminescence is unique in that it uses reduced flavin mononucleotide as a luciferin, which is abundant in all cells, making this system purely genetically encodable by the lux operon. Unfortunately, the use of bacterial bioluminescence has been limited by its low brightness compared with other luciferases. Here, we report the generation of an improved lux operon named ilux with an approximately sevenfold increased brightness when expressed in Escherichia coli; ilux can be used to image single E. coli cells with enhanced spatiotemporal resolution over several days. In addition, since only metabolically active cells produce bioluminescent signal, we show that ilux can be used to observe the effect of different antibiotics on cell viability on the single-cell level."],["dc.identifier.doi","10.1073/pnas.1715946115"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/11712"],["dc.language.iso","en"],["dc.notes.status","final"],["dc.title","Strongly enhanced bacterial bioluminescence with theiluxoperon for single-cell imaging"],["dc.type","journal_article"],["dc.type.internalPublication","unknown"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","26491"],["dc.bibliographiccitation.issue","52"],["dc.bibliographiccitation.journal","Proceedings of the National Academy of Sciences of the United States of America"],["dc.bibliographiccitation.lastpage","26496"],["dc.bibliographiccitation.volume","116"],["dc.contributor.author","Gregor, Carola"],["dc.contributor.author","Pape, Jasmin K."],["dc.contributor.author","Gwosch, Klaus C."],["dc.contributor.author","Gilat, Tanja"],["dc.contributor.author","Sahl, Steffen J."],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2020-04-03T13:38:03Z"],["dc.date.available","2020-04-03T13:38:03Z"],["dc.date.issued","2019-12-02"],["dc.description.abstract","Bioluminescence-based imaging of living cells has become an important tool in biological and medical research. However, many bioluminescence imaging applications are limited by the requirement of an externally provided luciferin substrate and the low bioluminescence signal which restricts the sensitivity and spatiotemporal resolution. The bacterial bioluminescence system is fully genetically encodable and hence produces autonomous bioluminescence without an external luciferin, but its brightness in cell types other than bacteria has, so far, not been sufficient for imaging single cells. We coexpressed codon-optimized forms of the bacterial luxCDABE and frp genes from multiple plasmids in different mammalian cell lines. Our approach produces high luminescence levels that are comparable to firefly luciferase, thus enabling autonomous bioluminescence microscopy of mammalian cells."],["dc.identifier.doi","10.1073/pnas.1913616116"],["dc.identifier.pmid","31792180"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/63635"],["dc.language.iso","en"],["dc.relation.eissn","1091-6490"],["dc.relation.issn","0027-8424"],["dc.relation.issn","1091-6490"],["dc.title","Autonomous bioluminescence imaging of single mammalian cells with the bacterial bioluminescence system"],["dc.type","journal_article"],["dc.type.internalPublication","no"],["dspace.entity.type","Publication"]]