Moneron, Gael

[["dc.bibliographiccitation.firstpage","571"],["dc.bibliographiccitation.issue","7"],["dc.bibliographiccitation.journal","Nature Methods"],["dc.bibliographiccitation.lastpage","573"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Han, Kyu Young"],["dc.contributor.author","Westphal, Volker"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Reuss, Matthias"],["dc.contributor.author","Engelhardt, Johann"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:44:10Z"],["dc.date.available","2017-09-07T11:44:10Z"],["dc.date.issued","2011"],["dc.description.abstract","Applying pulsed excitation together with time-gated detection improves the fluorescence on-off contrast in continuous-wave stimulated emission depletion (CW-STED) microscopy, thus revealing finer details in fixed and living cells using moderate light intensities. This method also enables super-resolution fluorescence correlation spectroscopy with CW-STED beams, as demonstrated by quantifying the dynamics of labeled lipid molecules in the plasma membrane of living cells."],["dc.identifier.doi","10.1038/NMETH.1624"],["dc.identifier.gro","3142707"],["dc.identifier.isi","000292194500020"],["dc.identifier.pmid","21642963"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/141"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1548-7091"],["dc.title","Sharper low-power STED nanoscopy by time gating"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","5225"],["dc.bibliographiccitation.issue","5"],["dc.bibliographiccitation.journal","Optics Express"],["dc.bibliographiccitation.lastpage","5236"],["dc.bibliographiccitation.volume","20"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Rittweger, Eva"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:58Z"],["dc.date.available","2017-09-07T11:48:58Z"],["dc.date.issued","2012"],["dc.description.abstract","In stimulated emission depletion (STED) nanoscopy the wavelength of the STED beam is usually tuned towards the red tail of the emission maximum of the fluorophore. Shifting the STED wavelength closer to the emission peak, i.e. towards the blue region, favorably increases the stimulated emission cross-section. However, this blue-shifting also increases the probability to excite fluorophores that have remained in their ground state, compromising the image contrast. Here we present a method to exploit the higher STED efficiency of blue-shifted STED beams while maintaining the contrast in the image. The method is exemplified by imaging immunolabeled features in mammalian cells with an up to 3-fold increased STED efficiency compared to that encountered in standard STED nanoscopy implementations."],["dc.identifier.doi","10.1364/OE.20.005225"],["dc.identifier.gro","3142577"],["dc.identifier.isi","000301053200043"],["dc.identifier.pmid","22418329"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/8943"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1094-4087"],["dc.title","STED with wavelengths closer to the emission maximum"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","4477"],["dc.bibliographiccitation.issue","15"],["dc.bibliographiccitation.journal","Chemistry - A European Journal"],["dc.bibliographiccitation.lastpage","4488"],["dc.bibliographiccitation.volume","16"],["dc.contributor.author","Mitronova, Gyuzel Yu"],["dc.contributor.author","Belov, Vladimir N."],["dc.contributor.author","Bossi, Mariano L."],["dc.contributor.author","Wurm, Christian A."],["dc.contributor.author","Meyer, Lars"],["dc.contributor.author","Medda, Rebecca"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Bretschneider, Stefan"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Jakobs, Stefan"],["dc.contributor.author","Hell, Stefan W."],["dc.date.accessioned","2017-09-07T11:46:10Z"],["dc.date.available","2017-09-07T11:46:10Z"],["dc.date.issued","2010"],["dc.description.abstract","New photostable rhodamine dyes represented by the compounds 1 a-r and 3-5 are proposed as efficient fluorescent markers with unique combination of structural features. Unlike rhodamines with monoalkylated nitrogen atoms, N',N-bis(2,2,2-trifluoroethyl) derivatives 1e, 1i, 1j, 3-H and 5 were found to undergo sulfonation of the xanthene fragment at the positions 4' and 5'. Two fluorine atoms were introduced into the positions 2' and 7' of the 3',6'-diaminoxanthene fragment in compounds 1 a-d, 1 i-l and 1 m-r. The new rhodamine dyes may be excited with lambda=488 or 514 nm light; most of them emit light at lambda=512-554 nm (compounds 1q and 1r at lambda=576 and 589 nm in methanol, respectively) and have high fluorescence quantum yields in solution (up to 98%), relatively long excited-state lifetimes (>3 ns) and are resistant against photobleaching, especially at high laser intensities, as is usually applied in confocal microscopy. Sulfonation of the xanthene fragment with 30% SO(3) in H(2)SO(4) is compatible with the secondary amide bond (rhodamine-CON(Me)CH(2)CH(2)COOH) formed with MeNHCH(2)CH(2)COOCH(3) to providing the sterically unhindered carboxylic group required for further (bio)conjugation reactions. After creating the amino reactive sites, the modified derivatives may be used as fluorescent markers and labels for (bio)molecules in optical microscopy and nanoscopy with very-high light intensities. Further, the new rhodamine dyes are able to pass the plasma membrane of living cells, introducing them as potential labels for recent live-cell-tag approaches. We exemplify the excellent performance of the fluorinated rhodamines in optical microscopy by fluorescence correlation spectroscopy (FCS) and stimulated emission depletion (STED) nanoscopy experiments."],["dc.identifier.doi","10.1002/chem.200903272"],["dc.identifier.gro","3142992"],["dc.identifier.isi","000277331000009"],["dc.identifier.pmid","20309973"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/456"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10 / Funder: DFG; Max-Planck-Gesellschaft"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","0947-6539"],["dc.title","New Fluorinated Rhodamines for Optical Microscopy and Nanoscopy"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.artnumber","e54421"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","PLoS ONE"],["dc.bibliographiccitation.volume","8"],["dc.contributor.author","Vicidomini, Giuseppe"],["dc.contributor.author","Schoenle, Andreas"],["dc.contributor.author","Ta, Haisen"],["dc.contributor.author","Han, Kyu Young"],["dc.contributor.author","Moneron, Gael"],["dc.contributor.author","Eggeling, Christian"],["dc.contributor.author","Hell, Stefan"],["dc.date.accessioned","2017-09-07T11:48:18Z"],["dc.date.available","2017-09-07T11:48:18Z"],["dc.date.issued","2013"],["dc.description.abstract","In a stimulated emission depletion (STED) microscope the region in which fluorescence markers can emit spontaneously shrinks with continued STED beam action after a singular excitation event. This fact has been recently used to substantially improve the effective spatial resolution in STED nanoscopy using time-gated detection, pulsed excitation and continuous wave (CW) STED beams. We present a theoretical framework and experimental data that characterize the time evolution of the effective point-spread-function of a STED microscope and illustrate the physical basis, the benefits, and the limitations of time-gated detection both for CW and pulsed STED lasers. While gating hardly improves the effective resolution in the all-pulsed modality, in the CW-STED modality gating strongly suppresses low spatial frequencies in the image. Gated CW-STED nanoscopy is in essence limited (only) by the reduction of the signal that is associated with gating. Time-gated detection also reduces/suppresses the influence of local variations of the fluorescence lifetime on STED microscopy resolution."],["dc.identifier.doi","10.1371/journal.pone.0054421"],["dc.identifier.gro","3142402"],["dc.identifier.isi","000313872800053"],["dc.identifier.pmid","23349884"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/7885"],["dc.language.iso","en"],["dc.notes.intern","WoS Import 2017-03-10"],["dc.notes.status","final"],["dc.notes.submitter","PUB_WoS_Import"],["dc.relation.issn","1932-6203"],["dc.title","STED Nanoscopy with Time-Gated Detection: Theoretical and Experimental Aspects"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.subtype","original"],["dspace.entity.type","Publication"]]