Czesnik, Dirk

[["dc.bibliographiccitation.firstpage","925"],["dc.bibliographiccitation.issue","4"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","934"],["dc.bibliographiccitation.volume","26"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Heermann, Stephan"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Brase, Christoph"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Roessler, Wolfgang"],["dc.date.accessioned","2018-11-07T10:59:55Z"],["dc.date.available","2018-11-07T10:59:55Z"],["dc.date.issued","2007"],["dc.description.abstract","The sensory input layer in the olfactory bulb (OB) is typically organized into spheroidal aggregates of dense neuropil called glomeruli. This characteristic compartmentalization of the synaptic neuropil is a typical feature of primary olfactory centres in vertebrates and most advanced invertebrates. In the present work we mapped the location of presynaptic sites in glomeruli across the OB using antibodies to presynaptic vesicle proteins and presynaptic membrane proteins in combination with confocal microscopy. In addition the responses of glomeruli upon mucosal application of amino acid-odorants and forskolin were monitored using functional calcium imaging. We first describe the spatial distribution of glomeruli across the main olfactory bulb (MOB) in premetamorphic Xenopus laevis. Second, we show that the heterogeneous organization of glomeruli along the dorsoventral and mediolateral axes of the MOB is associated with a differential distribution of synaptic vesicle proteins. While antibodies to synaptophysin, syntaxin and SNAP-25 uniformly labelled glomeruli in the whole MOB, intense synaptotagmin staining was present only in glomeruli in the lateral, and to a lesser extent in the intermediate, part of the OB. Interestingly, amino acid-responsive glomeruli were always located in the lateral part of the OB, and glomeruli activated by mucosal forskolin application were exclusively located in the medial part of the OB. This correlation between odour mapping and presynaptic protein distribution is an additional hint on the existence of different subsystems within the main olfactory system in larval Xenopus laevis."],["dc.identifier.doi","10.1111/j.1460-9568.2007.05731.x"],["dc.identifier.isi","000248963400013"],["dc.identifier.pmid","17666078"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7761"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/50808"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Wiley-blackwell Publishing, Inc"],["dc.relation.issn","0953-816X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Presynaptic protein distribution and odour mapping in glomeruli of the olfactory bulb of Xenopus laevis tadpoles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","8965"],["dc.bibliographiccitation.issue","26"],["dc.bibliographiccitation.journal","Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","8973"],["dc.bibliographiccitation.volume","30"],["dc.contributor.author","Breunig, Esther"],["dc.contributor.author","Manzini, Ivan"],["dc.contributor.author","Piscitelli, Fabiana"],["dc.contributor.author","Gutermann, Benjamin"],["dc.contributor.author","Di Marzo, Vincenzo"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Czesnik, Dirk"],["dc.date.accessioned","2018-11-07T08:42:06Z"],["dc.date.available","2018-11-07T08:42:06Z"],["dc.date.issued","2010"],["dc.description.abstract","Cannabinoids modulate the activity of many neuronal cells, among them sensory neurons in the olfactory epithelium. Here we show that the endocannabinoid 2-arachidonoyl-glycerol (2-AG) is synthesized in both olfactory receptor neurons and glia-like sustentacular cells in larval Xenopus laevis. Its production in the latter depends on the hunger state of the animal. The essential effect of 2-AG in olfactory receptor neurons is the control of odorant detection thresholds via cannabinoid CB(1) receptor activation. Hunger renders olfactory neurons more sensitive. Endocannabinoid modulation in the nose may therefore substantially influence food-seeking behavior."],["dc.identifier.doi","10.1523/JNEUROSCI.4030-09.2010"],["dc.identifier.isi","000279581500028"],["dc.identifier.pmid","20592217"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/6313"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/19629"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Soc Neuroscience"],["dc.relation.issn","0270-6474"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","The Endocannabinoid 2-Arachidonoyl-Glycerol Controls Odor Sensitivity in Larvae of Xenopus laevis"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","119"],["dc.bibliographiccitation.issue","1"],["dc.bibliographiccitation.journal","European Journal of Neuroscience"],["dc.bibliographiccitation.lastpage","128"],["dc.bibliographiccitation.volume","23"],["dc.contributor.author","Czesnik, D."],["dc.contributor.author","Kuduz, Josko"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T10:33:50Z"],["dc.date.available","2018-11-07T10:33:50Z"],["dc.date.issued","2006"],["dc.description.abstract","Nucleotides and amino acids are acknowledged categories of water-borne olfactory stimuli. In previous studies it has been shown that larvae of Xenopus laevis are able to sense amino acids. Here we report on the effect of ATP in the olfactory epithelium (OE) of Xenopus laevis tadpoles. First, ATP activates a subpopulation of cells in the OE. The ATP-sensitive subset of cells is almost perfectly disjoint from the subset of amino acid-activated cells. Both responses are not mediated by the well-described cAMP transduction pathway as the two subpopulations of cells do not overlap with a third, forskolin-activated subpopulation. We further show that, in contrast to amino acids, which act exclusively as olfactory stimuli, ATP appears to feature a second role. Surprisingly it activated a large number of sustentacular supporting cells (SCs) and, to a much lower extent, olfactory receptor neurons. The cells of the amino acid- and ATP-responding subsets featured differences in shape, size and position in the OE. The latencies to activation upon stimulus application differed markedly in these subsets. To obtain these results two technical points were important. We used a novel dextran-tetramethylrhodamine-backfilled slice preparation of the OE and we found out that an antibody to calnexin, a known molecular chaperone, also labels SCs. Our findings thus show a strong effect of ATP in the OE and we discuss some of the possible physiological functions of nucleotides in the OE."],["dc.identifier.doi","10.1111/j.1460-9568.2005.04533.x"],["dc.identifier.isi","000235052400013"],["dc.identifier.pmid","16420422"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7748"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/44713"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Blackwell Publishing"],["dc.relation.issn","0953-816X"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","ATP activates both receptor and sustentacular supporting cells in the olfactory epithelium of Xenopus laevis tadpoles"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","2967"],["dc.bibliographiccitation.issue","8"],["dc.bibliographiccitation.journal","PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA"],["dc.bibliographiccitation.lastpage","2972"],["dc.bibliographiccitation.volume","104"],["dc.contributor.author","Czesnik, Dirk"],["dc.contributor.author","Schild, Detlev"],["dc.contributor.author","Kuduz, Josko"],["dc.contributor.author","Manzini, Ivan"],["dc.date.accessioned","2018-11-07T11:04:55Z"],["dc.date.available","2018-11-07T11:04:55Z"],["dc.date.issued","2007"],["dc.description.abstract","The perception of odors is influenced by a variety of neuromodulators, and there is growing evidence that modulation already takes place in the olfactory epithelium. Here we report on cannabinergic actions in the olfactory epithelium of Xenopus laevis tadpoles. First we show that CB1 receptor-specific antagonists AM251, AM281, and LY320135 modulate odor-evoked calcium changes in olfactory receptor neurons. Second, we localize CB1-like immunoreactivity on dendrites of olfactory receptor neurons. Finally, we describe the cannabinergic influence on odor-induced spike-associated currents in individual olfactory receptor neurons. Here we demonstrate that the cannabinergic system has a profound impact on peripheral odor processing and discuss its possible function."],["dc.description.sponsorship","NIDA NIH HHS [DA 11322, R01 DA011322]"],["dc.identifier.doi","10.1073/pnas.0609067104"],["dc.identifier.isi","000244511200072"],["dc.identifier.pmid","17301239"],["dc.identifier.purl","https://resolver.sub.uni-goettingen.de/purl?gs-1/7749"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/51953"],["dc.notes.intern","Merged from goescholar"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.publisher","Natl Acad Sciences"],["dc.relation.issn","0027-8424"],["dc.rights","Goescholar"],["dc.rights.uri","https://goescholar.uni-goettingen.de/licenses"],["dc.title","Cannabinoid action in the olfactory epithelium"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dc.type.status","published"],["dc.type.version","published_version"],["dspace.entity.type","Publication"]]