Blaschke, Sabine

[["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","Journal of Arthritis"],["dc.bibliographiccitation.volume","5"],["dc.contributor.author","Vasko, Radovan"],["dc.contributor.author","Blaschke, Sabine"],["dc.contributor.author","Streich, Jan-Hendrik"],["dc.contributor.author","Müller, Gerhard A."],["dc.contributor.author","Korsten, Peter"],["dc.contributor.author","Dihazi, Hassan"],["dc.date.accessioned","2020-05-04T07:22:22Z"],["dc.date.available","2020-05-04T07:22:22Z"],["dc.date.issued","2016"],["dc.description.abstract","Background: To identify differentially regulated serum proteins, we compared proteome profiles of sera from patients with rheumatoid arthritis (RA) and healthy controls using proteomics. Methods: Sera were collected from 43 patients with RA and 48 healthy volunteers. The samples were cleared of the most abundant major proteins by immunoaffinity chromatography. Serum protein profiles between the two groups were compared by two-dimensional differential gel electrophoresis (2D-DIGE) and differentially regulated proteins were studied using mass spectrometry. Results: We identified 26 differentially expressed serum proteins between patients with RA and healthy controls. A quantitatively significant change of protein levels was defined as at least 1.5-fold upregulation or 0.6-fold downregulation respectively. Using these criteria, patients with RA exhibited significantly higher levels of leucine-rich alpha-2-glycoprotein (p<0.01), apolipoprotein A-IV (p<0.001), clusterin (p<0.001), haptoglobin (p<0.001), Ig alpha-1 chain C region (p<0.05), retinol-binding protein 4 (p<0.001), serum amyloid A (p<0.01) and alpha-1-antichymotrypsin (p<0.01). The levels of serotransferrin were significantly decreased in RA patients (p<0.01). Conclusion: We identified eight proteins with significantly increased and one protein with significantly decreased serum levels in RA patients compared to healthy controls. Several of these proteins may be implicated in the pathogenesis of RA and may have potential in diagnostics and activity assessment of RA."],["dc.identifier.doi","10.4172/2167-7921.1000201"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/64544"],["dc.language.iso","en"],["dc.relation.issn","2167-7921"],["dc.title","Comparative Serum Proteomic Analysis of Differentially Regulated Proteins in Patients with Rheumatoid Arthritis and Healthy Volunteers"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","48"],["dc.bibliographiccitation.journal","Deutsches Ärzteblatt international"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Korsten, Peter"],["dc.contributor.author","Blaschke, Sabine"],["dc.date.accessioned","2018-11-07T10:04:38Z"],["dc.date.available","2018-11-07T10:04:38Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.3238/arztebl.2016.0824"],["dc.identifier.isi","000391131600003"],["dc.identifier.pmid","28073427"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/38740"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1866-0452"],["dc.title","A 76-Year-Old Man with COPD and Acute Dyspnea"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.issue","41"],["dc.bibliographiccitation.journal","Deutsches Ärzteblatt international"],["dc.bibliographiccitation.volume","113"],["dc.contributor.author","Korsten, Peter"],["dc.contributor.author","Blaschke, Sabine"],["dc.date.accessioned","2018-11-07T10:07:02Z"],["dc.date.available","2018-11-07T10:07:02Z"],["dc.date.issued","2016"],["dc.identifier.doi","10.3238/arztebl.2016.0691"],["dc.identifier.isi","000389535300007"],["dc.identifier.pmid","27839540"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/39206"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1866-0452"],["dc.title","Left Flank Pain in a 73-Year-Old Man"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","513"],["dc.bibliographiccitation.issue","3"],["dc.bibliographiccitation.journal","CLINICAL AND EXPERIMENTAL RHEUMATOLOGY"],["dc.bibliographiccitation.lastpage","520"],["dc.bibliographiccitation.volume","34"],["dc.contributor.author","Vasko, Radovan"],["dc.contributor.author","Streich, J.-H."],["dc.contributor.author","Blaschke, S."],["dc.contributor.author","Mueller, Georg Anton"],["dc.contributor.author","Mai, Burkhard"],["dc.contributor.author","Kostrzewa, Markus"],["dc.contributor.author","Sparbier, Katrin"],["dc.contributor.author","Korsten, Peter"],["dc.contributor.author","Bohr, Stefan"],["dc.contributor.author","Dihazi, Hassan"],["dc.date.accessioned","2018-11-07T10:14:47Z"],["dc.date.available","2018-11-07T10:14:47Z"],["dc.date.issued","2016"],["dc.description.abstract","Objective To study the protein expression differences between primary fibroblasts explanted from synovial membranes of patients with rheumatoid arthritis (RA) and osteoarthritis (OA). Methods Fibroblast cultures were obtained from 10 patients with RA and 5 patients with OA. After two-dimensional gel electrophoresis, proteins were excised and identified using peptide mass fingerprint. Expression of selected proteins was subsequently examined by immunoblot. Furthermore, we examined the cellular lysates for the presence of citrullinated proteins. Results The study was designed to compare expression changes of the common proteins detected in all studied fibroblast cultures (i.e. detected in all patients samples). We totally identified 191 shared proteins between RA and OA fibroblasts. A significant difference was defined as at least 2-fold upregulation or 0.6-fold downregulation of protein expression. The most obvious alteration observed in RA was the appearance of several vimentin fragments not present in OA. We did not detect citrullinated proteins in lysates from RA fibroblasts. This corroborates the current assumption that fibroblasts are not able to citrullinate proteins by themselves and that invading macrophages play a central role in this process. Conclusion We demonstrated that fibroblasts from patients with RA, despite being grown under identical conditions, preserve a particular feature and generate vimentin fragments not present in fibroblasts from OA. Elevated levels of different vimentin fragments have been recently reported in several rheumatic conditions. Further studies are needed to elucidate the pathogenic mechanisms induced by vimentin fragments in RA."],["dc.identifier.isi","000376977900019"],["dc.identifier.pmid","27049516"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/40684"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.issn","1593-098X"],["dc.relation.issn","0392-856X"],["dc.title","Vimentin fragments are potential markers of rheumatoid synovial fibroblasts"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]

[["dc.bibliographiccitation.firstpage","98"],["dc.bibliographiccitation.issue","2"],["dc.bibliographiccitation.journal","European Journal of Emergency Medicine"],["dc.bibliographiccitation.lastpage","104"],["dc.bibliographiccitation.volume","21"],["dc.contributor.author","Korsten, Peter"],["dc.contributor.author","Sliwa, Bodo"],["dc.contributor.author","Kuehn, Matthias"],["dc.contributor.author","Mueller, Georg Anton"],["dc.contributor.author","Blaschke, Sabine"],["dc.date.accessioned","2018-11-07T09:41:57Z"],["dc.date.available","2018-11-07T09:41:57Z"],["dc.date.issued","2014"],["dc.description.abstract","Objectives In emergency departments (EDs), overcrowding, workload complexity, and cost containment represent current operational problems. In this retrospective observational study, we analyzed the effects of a professional quality management (QM) system on patient flow, diagnostic validity, and hospital costs. Materials and methods In 2005/2006, the main ED at the University Medical Center Goettingen was reorganized. A professional QM system according to DIN EN ISO 9001:2008 was introduced in 2008. In a retrospective observational study, we compared the number of cases, the spectrum of clinical diagnoses, the validity of diagnoses, and hospital costs in the ED before (2005) and 2 years after the introduction of the QM system (2010). Results In the ED at the University Medical Center Goettingen, the number of cases increased by 22.7% between 2005 and 2010. After the introduction of the QM system, a significant reduction in patients' length of stay within the ED was achieved (P < 0.001). Furthermore, the rate of diagnostic errors for patients assigned for admission within the ED could be reduced significantly (P=0.002). A reduction of patient-related hospital costs of 8.9% was achieved by restriction of diagnostic tests according to standard operating procedures for each emergency diagnosis. Conclusion The introduction of a professional QM system in EDs improves patient flow as well as quality of medical care and results in a significant reduction in hospital costs. Further analyses should evaluate the effects of QM on quality indicators in a prospective multicenter study. Validation of results has to be performed in a dynamic model for process simulation."],["dc.identifier.doi","10.1097/MEJ.0b013e3283613e8d"],["dc.identifier.isi","000332210100004"],["dc.identifier.pmid","24573191"],["dc.identifier.uri","https://resolver.sub.uni-goettingen.de/purl?gro-2/33847"],["dc.language.iso","en"],["dc.notes.status","zu prüfen"],["dc.notes.submitter","Najko"],["dc.relation.eissn","0969-9546"],["dc.relation.issn","1473-5695"],["dc.title","Impact of professional quality management on interdisciplinary emergency care units"],["dc.type","journal_article"],["dc.type.internalPublication","yes"],["dc.type.peerReviewed","yes"],["dspace.entity.type","Publication"]]